PERSONALITY TRACES IN HISTORY
BREEDING AND SEED PRODUCTION OF AGRICULTURAL CROPS
It is important to reveal the genetic base of breeding genetic material used for development of new breeding accessions among diverse Brassica oleracea L. (CC, 2n = 18). Traditional varieties, hybrids and new ones recently developed are the main genetic resources.
Classification of a collection with DNA markers enables to reveal valuable genotypes and establish the breeding accession pedigree that allows developing the new accessions with sustainable economically valuable traits. The use of microsatellite markers (SSR) in B.oleracea L. has shown high efficiency in discovering genetic polymorphism between varieties and within varieties as well. In this study, 16 primer pairs have been taken to amplify microsatellite loci of genomic DNA in national 24 breeding accessions of cabbage. On the basis of the data obtained the dendrogram has been constructed with use of Jaccard’s coefficient. All locistudied were high informative, where 14 out of 16 had a PIC > 0.5. As a result, the level of genetic polymorphism has reached 85.7%. The large cluster of head cabbages consists of three subclusters: mid-maturing and early-maturing accessions of white head cabbage, red head and savoy cabbages, late-maturing and midmaturing accessions of white head cabbage, respectively. Maximum genetic distance in the cluster of head cabbages was obtained between head cabbage ‘Slava 231’ and ‘Vertu 1340’ with genetic similarity 44.7%. The two varieties of red head cabbage ‘Gako 741’ and ‘Kamennaya Golovka 447’ were genetically similar at 71.1%. The relatively low genetic similarity of these varieties can be explained by that they belong to different varietal groups. The most genetically closest varieties were ‘Zimovka 1474’ and ‘Podarok‘ with genetic similarity 86,5%. Information on topologic differentiation obtained from cluster analysis can be the basis for selection of genetically valuable breeding material with the use of DNA markers (Marker Assisted Selection).
The culture of unpollinated ovules in vitro in summer squash was used to develop fully homozygous breeding lines with the aim of the speeding-up breeding program. As a result of assessment for economically valuable traits, the seven promising DH-lines obtained from summer squash accessions differed by fruit shapes and colours were selected out. All breeding lines produced showed high homogeneity that retained in following generations and also have an appropriate set of economically valuable traits. DH-lines belonging to female type have up to 96% female flowers and only 4% male flowers. It is very important for breeding when the male flowers appeared in two weeks just after the female flower began blooming. The development of morphologically abnormal female and male flowers, along with gynandromorphy flowers was noted on selected DH-lines. During vegetation period from 26 to 36 flowers appeared on the plant, where out of them 19-21 ones were normally developed female flowers, 3-5 ones were normally developed male flowers, and up to 10-11 ones showed an abnormal way of development. The percentage of abnormal flowers stayed invariable when growing in greenhouse condition with high humidity and temperature as well as in open field condition. As it was shown the development of deformed abnormal flowers inherited and manifested in the following generation after self-pollination. As a result of the study, the occurred anomalies in course of male and female flower development in summer squash (C. pepo L.,) DH-lines produced through a cultivation of unpollinated ovules in vitro were described in details for the first time.
VEGETABLE PRODUCTION
In terms of occupied areas, onion is on the third place in the world among vegetable crops, second only to tomato and watermelon. In Russia, onion is grown on an area of 88 to 96 thousand hectares of onions, and ranks third, yielding to cabbage and tomato.
The increase in the production of onions is not only due to the increase in the areas occupied by the crop, but also due to increased yields. This is achieved by introducing new highly productive varieties and hybrids into production, increasing the overall cropping culture and the level of cultivation technology. Along with the creation of heterotic hybrids at the present time, the Russian Federation also continues to select varieties that show high rates when grown in a oneyear crop. The results of production tests of a new onion variety of Forward Selected Breeding VNIIO a branch of the FGBNU FNCO has shown that it is promising because it has a high yield potential, high quality of bulbs and excellent preservation in conditions of repositories with controlled conditions. Characterized by mid-maturity maturation. The period from full shoots to mass lodging of leaves is 110 days. In relation to the longitude of the day, the forward sort refers to a group of long-day bows. The bulb is bilobate, rounded and rounded – flat, with an average mass of 90-120 g. The color of the dry covering scales is yellow-brown, the succulent scales are greenish. The taste is semi-sharp. The dry matter content in the bulb during the test years was 11.0-12.0%.
AGRICULTURALMANAGEMENT
AGROCHEMISTRY
PLANT PROTECTION
ISSN 2618-7132 (Online)