Relevance. Doubled haploids (DH-plants) are excellent material for genetic research and breeding due to their complete homozygosity. The genus Raphanus from the Brassicaceae family is the toughest to produce doubled haploid plants through isolated microspore culture in vitro (IMC). The study of the causes of disturbed root formation and the development of elements of this stage of technology will significantly increase the effectiveness of the IMC technology for European radish.

Methods. The study included three varieties from the collection of the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC): Teplichny Gribovsky, Rozovo-krasniy s belim konchikom and Rhodes. The experiments used a standard protocol for obtaining DH plants using IMC technology in a standard form and with a modification of the rooting stage. The solid MS medium (with agar 7g/L): MS without hormones, MS medium supplemented with IAA at concentrations of 0.5; 1 and 2 mg / L and liquid MSm medium supplemented with 0.1 mg / L kinetin were used for rooting of regenerated plants. All media were supplemented with 20 g/L sucrose. We used three types of techniques for transplanting plant explants onto a solid hormonefree MS medium: planting micro-shoots with their basal part immersed by 2-3 mm into the medium; planting in a well made in a nutrient medium using tweezers under sterile conditions; and landing on the surface of the medium without embedment.

Results. In this work, we studied the features of the stage of rooting of regenerated European radish plants in vitro conditions. The transplant technique has been proven to be important for the successful establishment of radish micro-shoots. Plant explants must be planted strictly on the surface of a solid hormone-free nutrient medium MS, without embedment. The use of tubes with bridges made of filter paper and MSm liquid medium with the addition of 0.1 mg/L kinetin for the induction of root formation also showed high efficiency. For plants prone to the formation of root-like structures (RLS) with secondary tumors (ST), multiple dissection of abnormal formations with successive transplants is necessary. Modification at the rooting stage of micro-shoots growing has increased the percentage of successfully adapted DH plants in vivo conditions from 0-14% to 95-98%.

Relevance. Study the selection material of cucumber on the basis of the hypocotyl length, as well as to assess the stability of this feature depending on the year of research and the timing of sowing.

Material and methods. Studies were conducted on the selection samplesof the laboratory cucumber breeding and seed production of Cucurbitaceae of FSBSI Federal Scientific Vegetable Center in 2019 and 2020 in terms of seedling branches winter greenhouse type "Richelle". Cucumber seedlings were grown without artificial illumination. Measurement of the length of the subfamily knee was performed at the stages of expanded cotyledons and the formation of the first true leaf.

Results. The length of the hypocotyl varied in the phase of expanded cotyledons – from 3 to 8 cm, in the phase of the first real leaf – from 6 to 18 cm. It was found that at the early stages of development, in the phase of expanded cotyledons, it is effective to reject plants with a hypocotyl length ≥5 cm. This will allow you to get rid of unwanted samples at the early stages of growing seedlings or to conduct selection within populations. The final assessment of the breeding material for lodging stability should be carried out in the phase of the first true leaf, since the most intensive growth of the hypocotyl was observed in the interphase period "expanded cotyledons – the first true leaf". During this period, it is possible to select non-lodging samples in a group that was characterized by an average hypocotyl (3.7-4.0 cm) for the expanded cotyledon phase. In breeding samples, depending on the year of research, the difference in the length of the hypocotyl in the phase of expanded cotyledons was 0.1-0.8 cm. All this indicates the stability of this feature and, accordingly, the possibility of selecting forms with the necessary parameters. A comparative study of cucumber seedlings of different sowing periods (1.5 months difference) revealed the samples most sensitive to low light. Promising samples were selected with a short hypocotyl in the phase of expanded cotyledons (< 5 cm), which are of interest for breeding for seedling resistance to lodging.

Relevance. High lycopene fruit content has been regarded as a very important genetic trait in tomato breeding. Use lycopene molecular markers in combination with conventional breeding techniques allowed us to create hybrids with high lycopene accumulation, excellent organoleptic qualities, high yield production and resistance to pathogens, and to effectively optimize our breeding programmes for commercial greehouses production.

Material and Methods. In this study tomato samples including selected lines and hybrids with various allelic combinations of genes determining carotene accumulation, and other genetic traits, such as disease resistance and yield production were tested. Introgression of spontaneous and induced mutations was used to increase carotenoid levels (og and hp) and improve fruit technological qualities (nor, alc, rin). The research material was tomato collection, mutants, breeding lines and hybrids listed in the State Register Russian Federation tomato hybrids of breeding SS Agrofirm "Ilyinichna" VNIIO branch of the All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center. DNA typing of fruit quality genes was performed at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus.

Results. New domestic hybrids for industrial greenhouses, which characterised by improved organoleptic qualities and technological traits were developed with the help of phasedcross-breeding that allowed to combine the genes nor, rin, alc, leading to an extension of the shelf life with the genes B, og, hp1, etc., contributing to an increase in carotenoid content in fruits. It was established that for targeted selection and hybridization, despite the negative influence of the nor, rin, alc genes it is possible to raise the level of carotenoids to average values. Correlation between lycopene concentration in fruits and high temperature and level of insolation was confirmed. It was shown that pink-fruited forms contain significantly more lycopenethanred-fruitedones. Different all eliccombinations of structural genes involved in carotenoids biosynthesis and regulatory genes that provided maximal accumulation of lycopene in hybrid swithred and pink fruits were revealed. Hybrids with the combination of high concentrations of sugar (° Brix), dry matter and maximal lycopene values, combined defining excellent taste were selected: Prekrasnaiya lady, Olya, Quadrille, Victoria. New F₁ hybrids one for industrial greenhouses: G950, G956, G960, Magistral and pink fruited G12897, surpassed the Dutch standard in productivity up to 21%, and in tastes/organoleptic qualities for 1-1.8 points.

Relevance. The creation of heterotic hybrids of watermelon is one of the modern breeding trends for solving the problem of providing the population of the Russian Federation with melon production. The process of creating heterosis hybrids can be accelerated by the assessment of the total combination ability (ОCS) of parental lines.

Materials and methods. The studies were carried out in the zone of the Volgograd Trans-Volga region at the Bykovskaya melon selection experimental station, in dry conditions in 2018 and 2019. The object of research was 9 paternal lines and 3 maternal lines of watermelon. The assessment of the general combining ability was carried out according to two indicators: yield and dry matter content.

Results. In the course of the research, the obtained F1 watermelon hybrids were assessed for a set of economic characteristics in comparison with the standards: Zenit, Sinchevsky and Barkhan. Based on the results of the work, F₁ hybrids were identified according to the length of the growing season, yield, and dry matter content. By selecting valuable genetic sources with a high GCS, it is possible to successfully conduct breeding to improve the economically valuable indicators of F1 watermelon hybrids. According to the results of studies on yield, both in 2018 and in 2019, heterotic hybrids were distinguished, obtained from crossing with the paternal lines Favorit, Stimul, Ikar, the average effect over two years ranged from 3.5-10.6 t/ha. Among the studied maternal lines, the line Chms-4 has a positive effect, the average effect was 1.2 t/ha. In terms of dry matter content, a positive effect was found in the paternal lines Stimul – 1.8%, Medunok – 1.6%, Favorit – 0.8%, and in the maternal line Chms – 41.8%

Conclusions. Based on the results of the analysis of the combining ability in terms of yield and dry matter content, we identified two promising paternal lines for creating heterotic watermelon hybrids: Favorite, Stimul. Also, one maternal line Chms-4 was distinguished. When using these lines in heterotic breeding of watermelon, in most crossing combinations, heterosis will be manifested in one or two characters.

Relevance. Root chicory is a valuable economic crop. However, its attractiveness for the producer is reduced due to the high labor intensity of cultivation and a high proportion of manual labor, primarily when harvesting root crops. Modern agricultural production is in urgent need of new varieties of root chicory that combine high yields and the necessary chemical and technological qualities, having the form of a root crop suitable for mechanized harvesting and adapted to cultivation in the soil and climatic conditions of the non-Chernozem zone of Russia. The purpose of this work is to test varieties of root chicory in a collection nursery in the Yaroslavl region.

Material and methods. The research was conducted at the the Yaroslavl region. The material for research was three varieties of Dutch selection Wixor RS, Rexor RS, Luxor. The experience is based on the "Guidelines for the study and maintenance of the world's collection of root crops", recommended by VIR. Vavilov.

Results. Investigated varieties in the soil and climatic conditions of the non-black earth zone of Russia were distinguished by a short root crop (up to 25 cm long) with a large diameter (up to 9 cm). We recommend them as donors in the selection of root chicory for suitability for mechanized harvesting. From long-term observations, it follows that the varieties Wixor RS, Rexor RS and Luxor are early-maturing. The length of their vegetative period does not exceed 125 days. This group of varieties is the most valuable when breeding for early maturity. Based on the analysis of the results of economic-biological and environmental studies of Dutch varieties of root chicory, it was found that they have high average values of certain economic-valuable characteristics and stability of their manifestation over the years.

Relevance. Development of valuable sterile lines of sweet pepper using nuclear-cytoplasmic male sterility is associated with the search for stable sterility maintainers in collection material.

Material and methods. The purpose of the research is to optimize the identification of the desired genotypes and accelerate the development of sterile lines using artificial climate chambers. The research material was a sterile line msTol55, which under the conditions of a film greenhouse showed partial fertility at the beginning or at the end of the growing season. The place of cultivation is artificial climate chamber, a film spring greenhouse.50-day-old seedlings were planted in pots on 10.01, the period of growing plants in the chamber - until 20.04, then the adult plants were transplanted into a spring film greenhouse. According to the traditional technology, the cassette seedlings of the same line were planted in the greenhouse on 15.04. The number of tested plants is 16-20 pcs. Growing mode in the chamber: 12 hours were illuminated with DRLV lamps, while the temperature was at the level of 26-28°C, from 20.00 to 8.00 the lamps were not illuminated, the temperature was 14-15°C. The actual sterility / fertility of the flowers was determined by the level of contamination of the fruits at the beginning of their formation.

Results. The maintained temperature regime for growing in an artificial climate chamber made it possible to identify sterile (30%) and partially fertile plants (70%) in the first 2 weeks of flowering. When adult plants were transplanted into a spring greenhouse, the number of sterile plants increased to 56%. When growing the line msTol55 in a spring greenhouse using the usual technology, partial setting of seeded and little seeded fruits on 60% of the plants was observed from the third decade of May to the second decade of July, then all plants set only non-seeded fruits. Based on the results obtained, it is advisable to identify the breeding material for the Rf gene in the ACC, since the proposed growing regime serves as a provocative background and allows for more effective control over the manifestation of sterility.

Relevance. The article presents the results of using three different selection methods to obtain new forms of tomato that combine high productivity with resistance to high-temperature stress factor.

Methods. Eleven hybrid combinations were used as experimental material. Selections were made starting with the offspring of the F1 generation. Three different methodological approaches were used: 1 – step-by-step alternation of sporophytic-gametophytic selections under high temperature pressure; 2 – only gametophytic selection using heat-treated pollen for pollination; 3 – traditional selections based on a high value of indicators of the studied traits. In each of the variants of the experiment, we studied: the heat resistance of offspring at different stages of ontogenesis – "sporophyte-gametophyte"; the duration of the vegetation period of plants; the fruit formation; the total yield. The offspring of the most stable and productive plants isolated from different hybrid combinations were combined in the populations in each variant of the experiment and a comparative analysis and evaluation of the effectiveness of the methods used was carried out.

Results. It is shown that the combination of step-by-step sporophyte-gametophyte selections under the action of high-temperature regimes (43°C and 45°C), at early stages (F1–F3) lead to more stable, productive offspring in F5-F7 both for sporophyte and gametophyte traits, compared to using only gametophyte selections and stronger differences relative to the control variant. The gradual alternation of selections at different stages of ontogenesis also leads to the production of offspring with a shorter period of passage of the phenophases "seedling-flowering" – "floweringmaturation" and a stable manifestation of signs that determine the quality of the fruits. Using only gametophytic selections and traditional methods of selection was less effective than alternating sporophyte-gametophyte ones.

Conclusions. The differentiating ability of the selection methods used and their influence on obtaining more resistance and productive genotypes was established. The advantage of the method of step-by-step alternation of sporophyte-gametophyte selections, which led to the production of new lines (118/1, 124/1, 133-11/1, 133-12/1, 134/1, 110/1 and 7/1) with high resistance at different stages of ontogenesis (sporophyte-gametophyte), shorter vegetation period and high fruit-forming capacity in three different years of research (2015-2017).

Relevance. In the consumer market, there is a popular pumpkin in portion sizes weighing 1.0 to 2.5 kg. To meet the needs of the population and producers in pumpkin fruits, F1 varieties and hybrids with high economically valuable traits, portion size and manufacturability in production and processing are needed.

Material and methods. The purpose of the research is to analyze, isolate and select the best breeding samples for creating parental lines of butternut pumpkin and large-fruited portion size.
The experiment was carried out at the selection plot of the FSBSI "Federal Scientific Rice Centre" from 2015 to 2019. The objects of research were cultivars, hybrid populations, cultivars and samples obtained from different sources.

Results. Lines with a number of economically valuable traits are highlighted, including portion size, productivity, biochemical parameters and commercial attractiveness of fruits. According to the length of the growing season, all inbred lines were divided into 3 groups: early maturing, mid-maturing, late maturing. In terms of productivity, the following lines stand out: KrChkh, BatS, La. The following lines were distinguished by the content of dry soluble substances (SRV, mg%): KrChkh, LaChkh, BatS, LaN. In muscat lines, the number of marketable fruits per plant varied from 3.3-5.7 pcs / plant. According to keeping quality, the test samples were divided into several groups: the least keeping quality among the studied samples was the line 15-5 (105 days); in fruits of lines 24-3, 16-5, 15-1, the safety of fruits increased to 116-126 days. The 28-2 line (162 days) was the highest keeping quality. The rest of the lines were attributed to medium bikes (133-148 days). All selected lines were characterized by a female type of flowering, multi-fruiting, short-leaved and good keeping quality of fruits. The KrChx line has a pronounced leaf aerenchyme. The organoleptic analysis showed that all the lines identified have a high score of 4.5-5. All commercially valuable traits in the lines are fixed with an inward.

Relevance. Increasing the resistance of vegetable plants to unfavorable growing conditions with the use of growth regulators that actively affect the seed quality, increase the survival rate of seedlings, stimulate the setting, formation and maturation of the crop is a significant reserve for increasing productivity with the already achieved level of intensification of their production.

Material and methods. The effect of verbascosides obtained by exhaustive extraction with 60% aqueous ethyl alcohol from the aboveground part of Verbascum densiflorum Bertol. (Scrophulariaceae) was studied on the germination, growth, development and yield of tomatoes, cucumbers, onions and white cabbage. Before sowing, the seeds were soaked in an aqueous solution of verbascosides for 15-20 minutes, followed by drying to flowability at the rate of 0.5-0.8 liters of solution per 1 kg of seeds.

Results. It is shown, that possessing phytoregulatory activity and influencing the metabolic and synthetic reactions of plants, these compounds, applied exogenously, stimulate the seed germination energy and total germination of vegetable crops, provide simultaneous appearance of seedlings and optimal plant density, accelerate the growth dynamics of plants assimilation surface and fruit mass. Reducing the consequences of stresses arising in the process of plant growth and development, they thereby contribute to increasing plant resistance, productivity and improving the commercial quality of the products. The ability of these compounds to improve the production process of vegetable crops, depending on the type and variety of vegetables was established. Their highest stimulating effect was determined on cabbage variants, where the yield exceeded the control by 34.3%. The performed studies and obtained results allow to recommend the pre-sowing seed treatment with a 0.01% solution of biological active substances from Verbascum densiflorum Bertol. as an element in technology of growing vegetable.

Relevance. Due to the wide diversity of cultivars and rootstocks, the selection of more productive, commercial and adapted graft-rootstock combinations for industrial orchards in the Orel region and the entire Central Chernozem region is relevant.

Materials and methods. The studies were carried out at the Russian Research Institute of Fruit Crop Breeding (VNIISPK) on the bases of the laboratory of cultivar study and cultivar agrotechnics of pome crops and the laboratory of physiology of fruit plant resistance. Winter apple cultivars of VNIISPK breeding ‘Sinap Orlovsky’, ‘Veteran’, ‘Orlik’, ‘Rozhdestvenskoye’, ‘Svezhest’, ‘Bolotovskoye’, ‘Veniaminovskoye’ and control cultivar ‘Antonovka Obyknovennaya’ on the vegetatively propagated semi-dwarf rootstock 54-118 were taken as objects of the studies. The orchard was planted in autumn 2013, the planting scheme was 6 mx3m.

Results. The studied apple cultivars have good compatibility with 54-118 rootstock, provide good tree development and low-sized crown. The highest yield on average for three years was in scab-immune apple cultivars ‘Bolotovskoye’ (4.6 t/ha), ‘Rozhdestvenskoye’ (3.4 t/ha) and ‘Venyaminovskoye’ (3.2 t/ha). The highest indicators for the load of the crop on the crown volume, crown projection area, and cross-sectional area of the stem were for ‘Svezhest’ and ‘Bolotovskoye’. As a result of determining the frost resistance of apple cultivars in mid-January to temperatures up to -38...-40°C, it was revealed that in the hardened state, the cultivars showed frost resistance of buds and tissues of annual shoots with reversible damage at -38°C in the middle of winter. A decrease in temperature to -40°C in January increased damage to buds and wood in the studied varieties, while the bark was characterized by greater frost resistance. According to the degree of damage to the cortex in January, when the temperature dropped to -40°C, ‘Rozhdestvenskoye’, ‘Svezhest’, and ‘Sinap Orlovsky’ were at the control level.

Relevance. The study of the resistance of garden cultures to unfavorable abiotic factors of the winter period is a priority direction of scientific studies, because the low winter hardiness can annul advantages the varieties on other signs. Concerning in the middle zone of Russia, studies on the resistance of cherry to frost during the thaw period remain relevant.

Methods. The studies were carried out in the laboratory of physiology of fruit plant resistance at VNI-ISPKin2017-2018. The object of research was the Turgenevka cherry variety on new clonal root-stock of the Institute breeding were studied. Control - Turgenevka on the Rubin stock. For artificial freezing in early December the material was prepared for III and IV components of frost hardiness.

Results. As a result of artificial freezing, the Turgenevka cherry variety was characterized by frost resistance of vegetative buds and tissues of annual shoots during a three-day winter thaw of + 2°C with a decrease in temperature of -25°C (III component of frost resistance). Inthiscase, thehighest frost resistance of the generative buds of the Turgenevka variety on rootstock 74340, 82987 is shown. After a three-day thaw of +2°C and repeated hardening with a subsequent decrease in temperature to -30°C in March (IV frost resistance component), the Turgenevka variety on the rootstock 74332, 74363, 82987 was characterized by frost resistance of vegetative buds, bark and wood of annualshoots. Moreover, in the studied scion-rootstock combinations, low frost resistance of the generative kidneys was revealed. Only in the studied variety on the rootstock 82987 was the largest percentage of living flower primordia revealed. The conducted studies revealed the Turgenevka variety in a rootstock of 82987 with the greatest potential for frost resistance during the winter thaws.

Relevance. In modern climatic conditions, the suppression of production process occurs against the background of short-term or long-term exposure to unfavorable environmental factors on agricultural plants, and, therefore, yield decreases. In this regard, there is a need for the use of growth regulators with protective and stimulating effects on many physiological processes.

The aim of the work was to study the influence of the element selenium and 6-benzylaminopurine on the production process of red currant.

Methods. Foliar treatments with solutions of sodium selenite and 6-benzylaminopurine (6-BAP) were made after flowering plants of the Dana and Asya varieties of the VNIISPK. The currant leaves were taken from annual shoots for determine the content of abscisic acid (ABA), the amount of pigments, and the rate of light reactions of photosynthesis. The parameters of the fruit brush of plants were recorded.

Results. It was shown that treatments with sodium selenite and 6-benzylaminopurine contribute to a decrease of 2.1-3.5 times in the leave tissues of the endogenous ABA level, increase the chlorophyll biosynthesis by 27.5-48.0% and accelerate by 19.3-45.8% electron transfer rate on the level of light photosynthesis reactions. Treatment with selenium and 6-BAP of Dana plants increased the average mass of the fruit brush by 19.30% and 24.5%, respectively, Asya varieties by 20.7 and 35.8%. The increase in the average mass of the fruit brush occurred due to the enlargement of the average mass of one berry in it. It was shown that selenium and 6-BAP in the Dana variety increased the weight of the berry by 27.3% and 47.3%, in Asya by 33.3% and 45.8%. The treatment did not have a significant effect on the amount of sugars. Thus, for increase the production process, it is advisable to make foliar treatments with sodium selenite and 6-BAP during the growing season on red currant plants.

Relevance. Unfortunately, there are no intensive pear plantations in the central regions of Russia due to the absence of intensive dwarf rootstocks. Therefore, the research aimed at obtaining winter-hardy rootstocks that provides early industrial fruiting of gardens, limits plant growth and gives a quick return on investment, is very important and can be applied in many different ways.

Materials and methods. The research was carried out in the laboratory of selection and variety study of pears and non-traditional pome crops of the Russian Research Institute of Fruit Crop Breeding (FSBSI VNIISPK). The object of the research was the common quince selected by FSBSI VNIISPK. In 2008-2010 and 2012-2014 we studied the economic and biological characteristics of the common quince as clonal pear rootstocks when propagated by green cuttings.

Results. The research results show high winter hardiness of the common quince plants in Oryol region, as well as their high regenerative capacity in case of damage caused by adverse winter conditions. It was determined that some types of common quince can be reproduced by green cuttings and provide a high yield of uniform planting material. The common quince has a high seed productivity that allows to get seedling rootstocks of high quality. Some pear cultivars demonstrate a good compatibility with the common quince, moderate growth and high early maturity in comparison with the pear rootstock; there are also preliminary data on compatibility of 11 pear cultivars in a nursery, and 4 cultivars that are incompatible with the common quince.

Relevance. A rare representative of the Apiaceae family the garden chervil is widely used abroad in nutrition and in folk and traditional medicine.

Methods. Evaluation of nutritional potential of Anthriscus cerefolium (L.) Hoffm and Anthriscus sylvestris Hoffm grown in Moscow region is achieved using the parameters of ascorbic acid, polyphenols, flavonoids, total antioxidant activity of ethanolic extracts.

Results. Garden chervil (Anthriscus cerefolium) was characterized by lower concentrations of ascorbic acid (1.6-1.8 times), phenolics (2.2-2.5 times), flavonoids (1.8-2.2 times), total antioxidant activity (1-1.54 times) and dry matter content (2.4-4.9 times) than wild chervil (Anthriscus sylvestris) but demonstrated significantly higher levels of total dissolved solids. Significantly higher content of dry matter in leaves and especially stems of wild chervil correlates with low nutritional value of stems despite large total mass of plants compared to garden chervil. Contrary to wild chervil, Anthriscus cerefolium recorded unusually high concentrations of carotene in leaves and significantly lower levels of chlorophyll b. Seeds of garden chervil were also characterized by low antioxidant status contrary to wild chervil and seeds of curly parsley. High yield and significant nutritional value of garden chervil indicate important prospects of its wide-scale cultivation in Russia while Anthriscus sylvestris plants seem to be important in breeding processes.

Relevance and methodology. Today, white cabbage is the most popular type of vegetable raw materials used for fermentation not only in Russia, but also in other countries. To obtain a variety of flavors and shades of flavor, various vegetables (carrots, sweet peppers), fruit (apples, cranberries) and spicy-aromatic additives (cumin, dill seed, etc.) are added to the cabbage during fermentation. During fermentation, the product contains not only the initial components of the raw material (vitamin C, macro-and micronutrients), but also significantly increases the number of lactic acid microorganisms – the main "participants" in the process.
One of the main products of the metabolism of lactic acid microorganisms is lactic acid. At a concentration of 0.5%, it begins to inhibit the development of many microorganisms. When reaching a concentration of 1-2% microbiological enzymatic hydrolysis, as a rule, ceases. The accumulation of acetic acid allows us to judge the intensity of the heterofermentative stage of fermentation using strains of lactic acid microorganisms.

Results. The L. plantarum + L. casei consortium has shown a synergistic interaction in the accumulation of lactic acid, since the amount of acid produced by the consortium significantly exceeds the amount of acid produced by each strain separately. This point is also noted for the accumulation of acetic acid. In the L. brevis + L. casei consortium, the opposite situation is observed: when using cultures separately, the accumulation of lactic acid occurs to a greater extent than when using two cultures simultaneously. There are no obvious differences between monocultures and the consortium in terms of acetic acid accumulation. In the case of the L. plantarum + L. brevis consortium, we observe a synergistic interaction, with the dominant role in this consortium played by L. plantarum, and the contribution of L. brevis to the accumulation of lactic acid is insignificant. At the same time, the total resulting accumulation of acetic acid is significantly less than the result of each strain separately, which may indicate a negative mutual effect of the participants in the process. Acid degradation of the polysaccharide matrix of cell walls does not occur during the entire fermentation process.

Relevance. The article presents the results of a study of the increase in the amount of reducing sugars in the inversion of sucrose due only to thermal effects during ultrasonic processing of vegetable and fruit monocomponent purees (with or without added sucrose). The relevance of the study is to identify the quantitative thermal component of a separate process in the total inversion of sucrose at the specified parameters of ultrasonic action on the product.

Material and methods. to perform this task, experimental samples of carrot, pumpkin and Apple puree with the addition of sucrose in an amount of 5% (by weight) and without it were previously made, which were then subjected to heat treatment at the same temperature and time exposure (at a temperature of 50 °C for 30 minutes). To study the dependence of the degree of sucrose inversion on the component composition of the processed product, the mass fraction of reducing sugars – fructose and glucosewas determined in the manufactured experimental samples of natural fruit and vegetable purees (without added sucrose) and in mixtures of purees with sucrose (according to a given recipe) before and after heat treatment at the specified parameters, depending on the recipe composition and pH of the medium.

Results. The obtained results of the study of the carbohydrate composition before and after thermal exposure show that the amount of reducing sugars in products subjected to heat treatment has significantly increased, compared to the initial content. Thus, in Apple puree with added sucrose, the mass fraction of fructose after heat treatment of the mixture increased by 197 mg per 100 g, i.e. by 3.8%, and glucose – by 192 mg per 100 g, i.e. by 6.7%. The total invert sugar content of the Apple mixture increased by 4.8 %. In the carrot mixture of puree with sugar, the content of total reducing sugars after thermal exposure increased by 5.4%. At the same time, in carrot natural puree, the increase in reducing sugars was only 3.6%, which is 1.5 times lower. In the pumpkin puree mixture with sugar, the total content of reducing sugars increased by 3.9%, and in the natural puree, the amount of invert sugars increased by only 2.7%, which is 1.4 times lower than puree with added sugar.

Relevance. Tomato root rot is a complex disease caused by a variety of soil-borne fungal pathogens, among which Fusarium oxysporum, F. solani and F. gibbosum occur with the greatest frequency in the conditions of the Republic of Moldova. The originality of the work consists in identifying the differentiated influence of pathogens of Fusarium root rot and temperature on the variability and heritability of the organs of growth and development of tomato plants.

Material and methods. Two varieties and 3 lines of tomatoes with good economically valuable characteristics were used as material for the study. To test the response of plants to pathogens of fusarium root rot, we used culture filtrates of 6 – F. oxysporum isolates, 6 – F. solani, and 4 – F. gibbosum, prepared in a standard liquid Czapek medium. The plants were grown at the optimum temperature (23...24°C) and with alternating temperatures of 23...24°C and 10...11°C for two days each. Seed germination and root length were used as indicators of growth and development. The genetic parameters of the variability and heritability of the studied traits were studied.

Results. An analysis of the reaction based on seeds germination and root growth showed its significant differentiation in the tomato genotype x type of fungus x isolate of the fungus x temperature system. A factor analysis of the interaction of the tomato genotype x Fusarium fungus revealed a significant role of the plant genotype in response to the pathogens. A significant effect of Fusarium fungi and temperature on the genetic and phenotypic variance of signs of growth and development of tomato plants, heritability in a broad sense, genotypic and phenotypic coefficient of variation, and genetic progress have been established. It is concluded that it is advisable to use the reaction of signs of growth and development of tomato to Fusarium pathogens in order to identify genotypes with good inheritance of the resistance trait.

Relevance. Garden strawberry (Fragaria x ananassa Duch.) is one of the most popular and widespread berry crops in the world. One of the reasons for the strawberries low yields is the loss of production from fungal diseases. Especially harmful is pathogens complex affect. One of the fusariosis distinctive features is a specific etiology – the participation of a different types complex of the genus Fusarium fungi in the pathogenic process, which differ in biological properties and are adapted to certain environmental conditions. The pathogen of Botrytis cinerea gray rot parasitizes the culture year-round. Various forms of strawberry root rot caused by pathogens of the genus Phytophthora are poorly studied diseases. Oomycetes of the genus Phytophthora are sources of transient forms of apparently healthy plants withering, associated with the appearance of horns necrosis. These processes occurrence is primarily due to the high harmfulness of these pathogens, that can cause death from 40 to 78% of strawberry plants during the growing season. The object of this work was to establish the species composition of the garden strawberries diseases in the Primorsky Territory and to develop recommendations on measures to control them.

Methods. The determination of pathogens was carried out by microscopy and passage of pure culture on differential media (Hella medium, carrot-sucrose agar) according to E. Blagoveshchenskaya (2015).

Results. It has been established that in the Primorsky Territory, gray rot, late blight and fusariosis received the highest prevalence among diseases of under coverage strawberry. The causative agent of late blight was previously defined as Phythophtora cactorum (Lebert &. Cohn) Schröd, of the gray rot as Botrytis cinerea, of the fusariosis as Fusarium oxisporum Schlecht. ex Fr. f. sp. fragariae Winks et Williams. Gumat K, Agat-25K, Switch 62.5 WG, Teldor 50 WG are effective drugs to contain its harmfulness and spread. As a result of the measures taken, the spread of the disease decreased to 40%, productivity increased by 1.5 times.

Relevance and methods. The structure of the food market is represented by various types of products, of which the largest share is in the fruit and vegetable sector. Given the tense political situation in the world and the establishment of barriers in foreign trade, agriculture is a strategically important sector of the Russian economy. Today, the fruit and vegetable branch of the Russian economy does not fully satisfy the population’s needs for fruits and vegetables. This is evidenced by the fact that the domestic market does not provide the population with high-quality vegetables and fruits, creates favorable conditions for filling it with foreign goods, which make up about 35% of the consumer basket of Russians. All this leads to the need for additional analysis to identify trends in the Russian market of fruits and vegetables and identify priority areas for its development. The purpose of the article is to identify the features of the functioning of the fruit and vegetable market in Russia. The study was based on statistics from the customs authorities of the Russian Federation on the import and export of fruits and vegetables, as well as the Federal State Statistics Service on production, consumption of fruits and vegetables in Russia. The article discusses the features of the functioning of the fruit and vegetable market in Russia. The key factors that influence the development of the market of fruits and vegetables at the present stage are determined.

Results. As a result of analysis of the state of the market of fruits and vegetables in Russia in 2011-2020. it can be stated that the fruit and vegetable market is experiencing difficult times due to the sanctions policy of the EU and the USA and the low level of material, technical and logistics base in Russia. The advantages of the market of fruits and vegetables during the period of antiRussian sanctions are reflected. Groups of problems of functioning of the market of fruits and vegetables are revealed. Based on the identified problems of the fruit and vegetable market, methods for solving them are proposed based on the best practices of the European Union. The article focuses on the development of the market for fruits and vegetables in Russia. The proposed solutions and development directions are substantiated by specific figures of economic efficiency.