Implementation of cell technologies has essentially improved the plant breeding process in agricultural crops in the world. The production of pure lines in cultivated crops, particularly among cross-pollinated species such as cucumber (Cucumis sativus L.) requires much time, labor and expense. Thus, the use of DH-plants for production of fully homozygous lines for one year becomes a very promising method for near cucumber breeding program. The major factor limiting the wide use of DH is a lack of effective protocol for large-scale plant production. In this review the historical facts with description of three main methods of DH-plant production were presented. By now these three methods have been such as parthenogenesis in situ induced by pollination with irradiated or chemically treated pollen; androgenesis in vitro including anther and isolated microspore cultivation in vitro; gynogenesis through ovule cultivation in vitro. Comparative analysis of published data with regard to the efficiency of the technology for DH-plant production was shown as well as advantages and limitations of each technology were described.

Relevance. Sterility is a very important trait that is indispensable for hybrid production. Genetic factor underlying in plant sterility can be now identified in large plant populations by DNA markers with high effectiveness and reliability. The evaluation of such markers enables to define their current applicability in breeding program.

Methods. The markers from different publications that had been successfully used were taken to test their effectiveness on 19 accessions of bulb onion (Allium cepa L.).

Results. Mitochondrial genes 5’cob, orf725 and orfA501 and alleles of fertility restoring locus Ms were also identified. Four breeding accessions had S-cytoplasm, nine accessions were with T-cytoplasm and six shared normal cytoplasm not showing any sterility gene in the analysis. As a result of marker testing, the all compositions of the genes in cytoplasm and Ms alleles in nucleus affecting the sterility had been revealed, such as one sterility maintainer, one male sterile accession, and two fertility restorers. However, it should be noted that not all markers tested were in accordance with each other, where the markers originated from chloroplast DNA of did not confirmed the results obtained with those cytoplasm-origins. As it was shown the practical use of the set of markers makes it possible to reveal necessary accessions with required gene composition for hybrid production in bulb onion.

Relevance. Melon as melon culture is cultivated in many countries of the world and is highly valued for its nutritional and dietary qualities. However, exposure to fungal diseases dramatically reduces the yield and taste of melons. Melon is affected mainly by powdery mildew and Anthracnose. In the conditions of the Bykovskaya melon breeding experimental station, the predominant type of powdery mildew is Sphaerotheca fuliginea. Evaluation and selection of the starting material with the identification of samples with complex resistance to these diseases is an important direction in the selection process, allowing to solve the problem of improving production efficiency and product quality.

Methods. The aim of this work is to obtain melon varieties and hybrids with complex resistance to powdery mildew and Anthracnose. Complex stability was determined by artificial infection of melon plants in the phase of 2-3 real leaves by conventional methods.

Results. 8 varieties and 10 F 1 hybrids of melon were studied for complex resistance to artificial Anthracnose and powdery mildew infection. Standard grade melon autumn was amazed powdery mildew of 81.2% with score lesion of 1.8 by Anthracnose, respectively, 93.8 per cent if the score is 2.3. The resulting hybrids were superior in stability to the standard. Hybrid x Ethiopian Farmer was amazed powdery mildew on 44% with score of 1.4. The prevalence of hybrid Original x Autumn was 54.4% powdery mildew with a score of 1.4, Anthracnose – 75% with a score of 1.8 (average for three years). The hybrid is fulfilled on economically valuable signs, has stable productivity of 17.5 t/ha, the content of dry substances in fruit juice to 17.2%. The samples and promising hybrids obtained with complex stability will be used in further breeding work.

Relevance. Each sample is characterized by a number of biological features, due to the recombination of genetic material in the process of its selection. These features are manifested in the reaction of plants to soil-climatic and technological conditions, expressed in the change of the elements of the yield structure and indicators of the level of adaptability of plants to growing conditions, as well as resistance to lodging. New samples have little-known properties that need to be studied and compared in specific soil and climatic conditions, to assess them in order to decide on the possibility of their zoning or culling. The main indicators characterizing the level of agricultural production are productivity, resistance to lodging, since the shortage of grain as a result of lodging of cereals can reach 15-40%. The introduction of new high-yielding varieties on the basis of their testing and the use of the most effective technological methods of cultivation on crops will allow to receive a significant gross harvest.

Materials and methods. The study was carried out in 2016-2018 to assess models of cultural hexaploid species of oats hulled and naked forms of resistance to lodging, yield and adaptability in the temperate continental climate of Central non-Chernozem zone. Resistance to lodging, yield variability, indices of environmental conditions and adaptability were determined. The main method of evaluation of the field resistance to lodging – visual (in points) that allows you to rank the varieties based on this characteristic. We were calculated potential productivity and adaptability of varieties by method L. A. Zhivotkov et al. [1], an indicator of the stability level of the sample yield (Puss) by the method of E. D. Nettevich et al. [2].

Results. Many varieties showed good plasticity and high productivity potential. The best in resistance to lodging, yield and adaptability among the hulled forms were varieties Sig, Mirt, Poseidon, Belinda, Rajtar, and the naked forms – Becas, Vyatsky, Korolek.

Relevance. Currently, there is an interest in this country in vegetable varieties of Vigna unguiculata subsp. sesquipedalis (L.) Verdc. However, a small number of varieties adapted to different cultivation conditions prevents a wider distribution of this crop. The present work was aimed at creating new vegetable cowpea varieties adapted to the climatic conditions of southern regions of Russia.

Materials and methods. The study was carried out in 1998-2018 in the Astrakhan Province (Astrakhan Experiment Station) and in 2012-2018 in Primorsky Territory (Far East Experiment Station). Vegetable cowpea accessions from the global VIR collection were studied.

Results. Many years of breeding have resulted in creation of such varieties as ‘Astrakhanskaya krasavitsa’, ‘Kaspiyskaya zarya’, ‘Zhemchyuzhina Kaspiya’ and ‘Lyanchihe’, which were included in the State Register of Breeding Achievements in 2019. All varieties are characterized by earliness, high productivity, excellent quality of vegetable pods, high protein content in pods in the technical ripeness phase (29.5-33.5%), and by low demand to the soil fertility. ‘Zhemchyuzhina Kaspiya’, ‘Astrakhanskaya krasavitsa’ and ‘Kaspiyskaya zarya’ are tolerant to high temperatures, air drought and soil salinity. The growing season for these varieties lasts 62-68 days in the Astrakhan Province under irrigation, while technical ripeness of pods occurs in 46-50 days. The green pod yield is 16.1–28.2 t/ha and the seed yield is 1.91-2.71 t/ha. The ‘Lyanchihe’ variety tolerates humidity well; its growing season in southern Primorsky Territory is 97-110 days, and pods reach technical ripeness in 61-70 days. The green pod yield is 21.0-24.0 t/ha, and that of seeds is 2.38-2.64 t/ha. The released varieties will broaden the range of vegetable crops cultivated in this country and improve the quality of nutrition of the population.

Relevance. Tomato is one of the most spreaded vegetable cultures in Khabarovsk Territory. Presence of the favourable weather conditions in the region, high ecological plasticity of tomato sorts promote the tomato cultivation in open and protected ground everywhere and in large areas. The main cause of crop failure or large losses of tomatoes in the Far East is a complex, unpredictable, not meeting anywhere else in Russia the monsoon climate causing a strong waterlogging observed almost every year in July-August, the period of the main tomato harvest forming. Such weather conditions promote the development of main diseases of the solanaceous cultures, and their most aggressive races often appear. Therefore a high natural infectious background and a sharply variable hydrothermal regime are the main limiting factors in the cultivation of tomatoes in the Khabarovsk Territory.

Methods. Work on the creation of a new variety with the given parameters was started in 2007 by conducting intervarietal crossings between Volgogradsky 5/95 and Zarya Vostoka varieties, followed by multiple selections against a natural infectious background. The early ripening variety of Zarya Vostoka is a local selection variety, was used in the breeding process as an early maturity donor, and Volgogradsky 5/95, a stem type of plant. The main method of breeding work to create new varieties of tomato was analytical with continuous selection. All the necessary phenological, biometric and phytopathological observations and counts according to methods generally accepted in this culture. The purpose of our research was to create a new strain tomato variety with a short period of vegetation, forming the harvest of fruit before the appearance and spread of the disease and high productive potential.

Results. This publication presents the information about the new local tomato sort Galant. This sort was received by inter-sort hybridization between the stem sort Volgogradsky 5/95 and local early ripening sort Zarya Vostoka. The sort was selected as a result long-term selection on the natural infectious background with the assessment by progeny of promising lines for a complex of morpho-biological and economically valuable traits in monsoon climate conditions of Priamurye. The new sort is different from other studied non-district and local sorts by fastripeness, high productivity, resistance to main diseases and also by stem type of plant.

Relevance. Melon is one of the most common cultures among melons. Bykovskaya for melon breeding research station scouting for new varieties of melon. When breeding melons, it is necessary to pay attention to such economic characteristics of melons as precocity, productivity, high quality products, resistance to disease, cold resistance.

Methods. Studies were carried out in 2017-2018 at the Bikovskaya experimental station, in rainfed conditions. Object of study – varieties and hybrid populations of melon.

Results. As a result of selection work the new variety sample of melon of Syngent x Dune is received. In 2017, this population was first included in the station variety testing. According to the research results, the new hybrid population Matures before The standard of the autumn variety for 6 days. The excess in yield over the standard is 6.6 t / ha. The taste of the new variety sample is at the level of standard varieties Osen. Thus, the variety sample of Syngent x Dune has a beautiful appearance, high yield of marketable products, good taste of the fruit, resistance to sunburn, good transportability, which will diversify the range of melon varieties grown in Russia.

Relevance. The main producers of cabbage in Dagestan are personal subsidiary farms of the Mountain subprovince. An additional reserve of production is the Foothill subprovince (in particular buinak district), soil and climatic conditions which are favorable for growing cabbage. In this regard, studies aimed at studying the adaptive potential of cabbage varieties in this subprovince are relevant.

Material and methods. The aim of the work is to develop elements of technology for growing varieties of cabbage in relation to the Foothill sub-province of the Republic of Dagestan. The results of three-year studies on the adaptive potential of cabbage varieties in irrigation conditions of the Foothill sub-province of the Republic of Dagestan are presented.

Results. As a result of the conducted researches it is revealed that the variety Nadezhda has formed the greatest productivity on all variants on a mode of irrigation. Quite high data are also noted in the variety Samur 2. Comparative analysis of variants of the experiment the total water consumption showed that, on average, for the studied varieties of cabbage most economical use observed in the irrigation regime providing for irrigation when pre-irrigation moisture threshold 75-85-75% NV. On average, the studied varieties and hybrids of cabbage, the most economical expenditure was observed in the irrigation regime, which provides for the appointment of watering at the pre-watering threshold 7585-75% NV. Compared with the first option (70-80-70% NV) saving irrigation water was 150 m³/ha, and compared with the third option (80-90-80% NV) – 178 m³/ha. On the same option was formed quite high productivity of varieties of cabbage.

Relevance. Widely used traditional wine varieties of European-Asian grapes are characterized by a low level of resistance to disease and require multiple treatments with pesticides to preserve the crop and plants. Obtaining and introduction of sustainable varieties into production is one of the urgent problems of modern viticulture in the world and in our country, especially in the aspect of the ever wider dissemination of the principles of organic production. This is especially true for vineyards located in the coastal areas of the South of Russia, where the use of methods of chemical protection of vineyards from pests and diseases are limited to the proximity to the sea coast and the status of the resort region.

Material and methods. The paper presents the results of the study of white wine grapes of the new generation with a high potential for resistance to environmental conditions and diseases. Purpose: research and development of recommendations on the use of white wine grapes of the new generation, characterized by resistance to adverse environmental conditions.

Results. It is revealed that the experimental white wine varieties are characterized by a high level of adaptive capacity. In terms of productivity, new varieties are not inferior to the Sauvignon variety. Wine materials made from new resistant varieties are characterized by a high content of extractive and phenolic compounds. According to the organoleptic characteristics of wine materials from new resistant white grape varieties are at the level of the control variety. It is established that the level of stability of new grape varieties can be excluded from the technology of care of the vineyard measures to protect against major diseases, which significantly reduces the cost of production and allows you to get environmentally safe and organic products.

Relevance. Currently, in many countries of the world, the production of non-season raspberry berry products has become widespread. Recently, interest in this technology has arisen in Russia, which has great prospects for the development of industrial gardening. In our opinion, it is promising to develop elements of technology for the non-seasonal production of red raspberries, propagated by the method of clonal micropropagation with a traditional and remontant type of fruiting in the conditions of winter heated greenhouses.

Material and methods. The experiments were carried out in the laboratory of clonal micropropagation of garden plants in the fruit growing laboratory of RGAU-MSHA named after K.A. Timiryazev. The objects of research were varieties of red raspberries with a traditional (variety Volnitsa) and remontant (varieties Orangevoe Chudo and Bryanskoe Divo) type of fruiting. The experimental plants were propagated by the method of clonal micropropagation and grown before distillation in open and protected ground; plants propagated by root offspring served as control. Experimental plants were planted in open ground for growing in mid-May, in mid-October they were transplanted into 10 liter containers and transferred to protected ground conditions. Then put in the refrigerator compartment with a temperature of + 1 ... + 5°C. For distillation, the raspberry repairing plants were exposed in the winter heated greenhouse on January 20, while the shoots of replacing the aboveground system were normalized: without normalization, 3 shoots per plant, complete pruning of the aboveground system. Raspberries with a traditional type of fruiting were exposed in a winter heated greenhouse in three periods on January 20, February 10, March 2. Accounting for the passage of the phenological phases of development and yield was made for 3 months every 5 days.

Results. In the conditions of winter heated greenhouses, efficiency has been shown and elements of technology for non-season production of raspberry berries remontant and berries with a traditional type of fruiting, propagated in vitro and grown before open field distillation are developed. It was revealed that it is necessary to normalize the shoots before distillation of raspberry remontant, and the optimal timing for the start of distillation for raspberries with a traditional type of fruiting has been established.

Relevance. One of the priority trends in berry crop breeding is to create highly adaptive and resistant to biotic and abiotic environmental factors varieties with high commodity and consumer quality of berries, not inferior to the best Western European varieties.

Material and methods. The research was conducted in 2017-2018 at VNIISPK (Orel) on the selection plot of red currants, planted in 2012, with a planting scheme 2.8 x 0.8 m. The research object was the selection red currant family 2466 (♀Belaya Potapenko x ♂SS 1426-21-80) of VNIISPK breeding in the amount of 66 hybrid seedlings. The parent forms of this family were selected as sources of valuable economical and breeding characters. The variety Belaya Potapenko has a dessert taste of berries, and the selected seedling 1426-21-80 was taken due to its productivity and long racemes. The economical and biological characters of the hybrid seedlings were studied according to the “Program and methods of fruit, berry and nut crops variety investigation”, section “Currants, gooseberry and their hybrids” (1999).

Results. The progeny of the crossing combination Belaya Potapenko x SS 1426-21-80 is characterized by high adaptability to soil and climatic conditions of cultivation that provides good development of the seedlings and their high productivity. When crossing white-fruit Belaya Potapenko with redfruit SS 1426-21-80, seedlings with white berries are absent in the offspring, which indicates that the paternal original form is homozygous for the dominant gene that controls the red color of the berries. Hybridological analysis showed that with medium weight of berries of parental forms, the manifestation of this trait in the offspring increased, the share of large-fruited seedlings was 18-37%, depending on the year of the study. The taste estimation of the off-spring of this family showed that a great amount of the seedlings had an intermediate sweet and sour taste, part of hybrids inherited sour taste from the paternal original parent. The hybrid family is valuable for the output of highly productive large-fruited seedlings with long racemes. Re-hybridization of the best seedlings with sources of high taste of berries is necessary to improve the taste.

Relevance. A wide range of pharmacological effects of tansy and Echinacea purpurea determine the continuing interest in the study of these cultures. Medicinal crops contain not only a large number of biologically active substances, but also trace elements necessary for normal growth and development. Agronomic bio-enrichment of medicinal raw materials allows you to increase productivity, manage product quality in the direction of enhancing the pharmacological action. Thus, the aim of the work was to study the effect of various doses of zinc acetate on the productivity of two medicinal crops of tansy and Echinacea purpurea.

Material and methods. Studies were conducted from 2012 to 2018 on meadow-chernozem soil of the southern forest-steppe of Western Siberia. As objects of research, perennial medicinal crops were chosen – Tanacetum vulgare L. and Echinacea purpurea L. Zinc fertilizers were applied in the background (N₁₂₅, N₁₃₅P₄₅K₄₅) used in acetate form.

Results. The introduction of zinc fertilizers into the soil contributed to an increase in the yield of medicinal crops in the conditions of the south of Western Siberia. It was revealed that the maximum yield was observed on the variants of 0.75 MAC Zn (Tanacetum vulgare L.) and 0.5 MAC Zn (Echinacea purpurea). Thus, tansy was more responsive to the use of micronutrient fertilizers. The studies established a high correlation relationship (r = 0.88–0.99) between yield indicators and doses of zinc added. The obtained optimal doses of zinc fertilizers (60 and 21.4 kg / ha) can be recommended for growing these crops in order to increase their productivity.

Relevance. Healthy eating is one of the many steps that lead to a healthy nation. It implies the provision of the human body with all the necessary elements for its normal functioning. Within the framework of import substitution, it is extremely important to identify and popularize valuable crops that are suitable for cultivation in the territory of the Russian Federation. One of these rarely studied and insufficiently studied cultures is the leaf variety of beet –chard (Beta vulgaris L. ssp. сicla). Man uses in the food the fleshy petioles and leaves of this plant.

Material and methods. The purpose of the research was to identify the characteristics of the biochemical composition of chard and compare them with the widespread red beet. The object of the study was 44 accessions of red beet (Beta vulgaris L. ssp. vulgaris) and 32 accessions of chard from the VIR collection, differing in phenotype and origin. The accessions were cultivated in the vegetable crop rotation in the Research and production base “Pushkin and Pavlovsk laboratories of VIR” (Pushkin, Leningrad region) for two years (2014 and 2015). Biochemical analysis was performed using standard methods. The quantitative and qualitative composition of the metabolites was assessed using GС-МS (Gas Chromatography-Mass Spectrometry).

Results. The results of the study revealed significant variability of indicators depending on the specific genotype. The indicators of the content of ascorbic acid, protein and pigments: betanin in beetroot and β -carotene in leafy can be attributed to slightly varying. A detailed comparative description of the content of carbohydrate profile metabolites is given. It is shown that chard is better balanced in terms of monosaccharides content, differs in low sucrose content. The amino acid composition of chard is richer and more diverse than that of table beet, it is significantly ahead of table beet in essential amino acids for humans. Selected accessions of chard are recommended for use in infant, dietary and diabetic nutrition. Due to the high protein content (83% higher than that of red beet), the culture is recommended as an ingredient for feed production. Leaf beet has a number of valuable advantages: unpretentious in cultivation, decorative and, most importantly, has great potential for use in human nutrition and fodder production.

Relevance. Carbohydrates are integral components of the cells and tissues of all living organisms of the plant and animal world; they are important components of the plant cell walls, as well as the extracellular matrix of animal and human tissues. The type of monosaccharide composition of polysaccharides determines their biological functions for the organism. However, it is difficult to establish the relationship between the structure of the carbohydrate molecule and its biological function.

Methods. In this article, the author provides an overview of methods for identifying the monosaccharide composition of plant polymers by capillary electrophoresis, in order to optimize the sample preparation procedure and the conditions of analysis. A scheme for the stepwise release of polysaccharides from raw materials is given: the isolation of soluble monosaccharides and polysaccharides, the extraction of the pectin fraction, the extraction of cellulose and hemicelluloses, in order to study the composition of each fraction. A procedure for acid hydrolysis of polymers to monosaccharides using oxidizing agents such as sulfuric and trifluoroacetic acid is described. In the final part of the article, three different schemes for the identification of the monosaccharide composition were analyzed, namely, separation of the components under strongly alkaline conditions, preliminary derivatization with 1-phenyl-3-methyl-5-pyrazolone, preliminary preparation of (S) (-) α-methylbenzylamine S-PEA) derivatives.

Results or Findings. An analysis of the literature shows that the composition of polysaccharides is widely studied by various methods. At the same time, a number of problems remain associated with the implementation of these methods in terms of sample preparation and identification of all monosaccharides characteristic of the studied raw materials. The method of capillary electrophoresis can solve some of these problems; however, little has been studied. The study of carbohydrates in plant objects begins with the procedure for extracting carbohydrates (polysaccharides and monosaccharides) from the plant matrix, hydrolysis of polysaccharides, and subsequent identification using the CE method. The optimization of this scheme of the CE method for determining the composition of polysaccharides is an urgent scientific task.

Relevance. Leuconostoc mesenteroides, Lactobacillus plantarum and Leuconostoc fallaxare the three main lactic acid bacteria developing in the production of sauerkraut. The process of production of fermented foods, as well as their characteristics are determined by the fermenting activity of microorganisms used. Many food products (matured cheeses, sauerkraut, etc.) are canned products: for them, the term is significantly increased compared to the shelf life of the raw material from which these products are made. Their characteristic feature is not only high stability during storage, but also a pronounced flavor bouquet, due to the direct or indirect action of fermenting microorganisms.

Material and methods. Microbiological methods of preserving fruits and vegetables are based on the formation of natural preservatives – lactic acid and alcohol, which accumulate as a result of fermentation of sugars by lactic acid bacteria or yeast. The intensity of lactic acid fermentation and the amount of accumulated lactic acid depend on several conditions: the presence of lactic acid bacteria, the content of sugars and other chemicals in the raw material, the addition of spice-aromatic plants, anaerobic conditions, temperature. Due to the accumulation of lactic acid, the development of other microorganisms, and then the lactic acid bacteria themselves, is suspended. The dynamics of development of lactic acid microorganisms and their consortia on the model media, pretreated by the culture of Leuconostoc mesenteroides species, at the main stage of step fermentation of white cabbage of the "Parus"variety are investigated.

Results. Dynamics of development of monocultures of L. brevis, L. casei and L. plantarum and their paired consortia at the main technological stage at two stages of fermentation are investigated. It is established that a preliminary fermentation of a model medium with a culture of Leuconostoc mesenteroides generates adverse conditions for the development of L. brevis and L. casei in the monoculture (pseudotensorial) and in consortium with each other, but favorable conditions for the development of L. plantarum in pseudotensorial and in pairwise combinations with L. brevis and L. casei.

Relevance. In many ways, a successful purchase and sale transaction is determined by the time it takes to complete it. The search for the best deal time is linked to the optimization of the time lag. The purpose of the study is to optimize the time lag of the agricultural market.

Methods. This article considered a role of time in business processes for Russian potato market of producer into period 1998-2018.

Results. There was trend of annual reduction about 365 thousand tons (1.2...1.7% of total) per year supply of potatoes on Russian domestic market of producers. Some priority of quantity vs price of producer been revealed. There was inverse proportional function between producers` prices and quantity of potatoes. That is than worse harvest that higher producer`s price. Reducing a supply of potatoes 1 million tons from agricultural market perhaps increase in producer prices by $ 8.5 / t (4...5% on average). Offered concept and hypothesis about linearization of equilibrium of market processes into conditions of time shifts of dynamics their parameters were confirmed. There verified assumption about existence of inter-interval time optimum which this linearization maximal business processes. In course of correlation analysis of dynamics of annual indicators of potato market, it revealed month May of current period as optimal time of producer's offers with price restrictions and diminishing returns, and month September with quantity restrictions and increasing returns. If fixation of producer`s prices situation of deals May more likely, in case of crop failure (limited quantity) in September.