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Vegetable crops of Russia

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No 3 (2016)
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BREEDING AND SEED PRODUCTION OF AGRICULTURAL CROPS

3-11 977
Abstract
On the second of October, in 1881, Sergey Ivanovich Zhegalov, an establisher of national breeding and seed production of vegetable crops was born in a little village Vasilkovo of Vyazemskogo uezda. He was a founder and a first director of Gribovskaya Vegetable Breeding Station. This year marked by 135th anniversary from the date of birth of the outstanding scientist. All the time at All-Russian Research Institute of Vegetable Breeding and Seed Production (VNIISSOK), its scientific leader and mastermind is honored and remembered for his heritage that is still preserved and
augmented. This scientist was at the beginning of plant breeding science and became the first who brought scientifically proved methods into agricultural plant science. The process of newplant- form-producing and development of new more qualified breeding forms through distant crossing, hybridization, heterosis effect, are the problems which interested the mind of Zhegalov and always were the sense of his life. These problems still remain in these days, where his ideas are embodied in scientific program of the institute covering theoretical researches for development of innovation method needed for creation of new highly qualified breeding plant material regarded as a source for nearest breeding practice and seed production. At VNIISSOK the richest plant collection with important genes and donor genotypes of productivity, fast ripening, high quality, resistance to biotic and abiotic stresses is created in Cucurbitaceae, Solanaceae,
Alliaceae, Fabaceae, root vegetables, Brassicas, leafy crops, aromatic and medicinal crops and ornamental crops. Core plant collection is substantially extending by means of introduction of new crops and non-traditional ones as well. The specialist-breeders of VNIISSOK have developed over 800 cultivars and hybrids F1 of vegetables, melons and gourds, aromatic plants, ornamental plants, non-traditional. 546 accessions out of 118 crops have been included into State Register of Breeding Achievements of Russian Federation in 2016.
12-17 1014
Abstract
A model of late maturing F1 hybrid of white head cabbage for long-term storing and suitable for national recipe “golubci” have been developed based on requirements for varieties and F1 hybrids grown in the South in the conditions of drought, high temperatures and low air humidity. Combining ability of seven self-incompatible inbred lines of white head cabbage was studied by the system of full diallel crosses in conditions of Pridnestrovian Moldavian Republic. These lines were developed on the base of heat tolerant varieties ‘Biruchekutskaya’, ‘Volna’, ‘Lada’, ‘Moldavanka’ and ‘Kharkovskaya Zimnyaya’ bred in the south. Out of 42 hybrid combinations studied 15 significantly surpassed in productivity the variety ‘Zavadovskaya’ that is traditionally grown by local farmers for pickling and recipe “golubci”. High GCA effects for productivity showed the following breeding lines: Bu1, Ml3 and Kl5. Polygenes, controlling the high yield ability, mainly were dominant and single-directed, however the correlation between
yield ability and GCA effect was middle, r=0.63±0.35. No correlation was observed in
breeding line between GCA effect and truly heterosis effect in hybrid combinations (r=0.19). Disease resistance analysis revealed lines Bu1 and Kl5 that had shown resistance to Fusarium yellows. Two hybrid combination Kl5xBu1 and Ml3xBu1 were highly tolerant to thrips attacks according to analysis of all promising hybrid combinations, carried out in natural infection background. Weak correlation was observed between the number of damaged leaves in cabbage head and dry matter content, r=0.41±0.21, and also there is no correlation revealed with sugar contents, ascorbic acid and nitrate content. High tolerance of hybrids with participation of line Bu1 explained the partial dominance nature of the trait. Hybrid combinations Kl5xBu1 and Ml3xBu1 called ‘Batal’ and ‘Shedevr’, respectively, included in registry of Moldova and PMR.
18-23 1189
Abstract
The vegetable marrow and pumpkin are valuable vegetable crops thanks to high food and dietary qualities of fruits. Russia is on the third place in the world on gross yield of these crops, but on productivity takes the 11th place among the European countries. The state register of breeding achievements permitted for utilization in the territory of the Russian Federation for 2016, contains 152 varieties and a hybrids of vegetable marrows and the 138 pumpkins. But there are not enough varieties of a wide area, there are practically no ultra early varieties, there are not enough pumpkin varieties with a bush habitus of plants. The collection of pumpkins and vegetable marrows which is a source of valuable genotypes for the solution of actual tasks of breeding contains 2641 accessions from 99 countries of the world and wins the first place in the number of accessions among world genebanks. The greatest part of a collection (more than 1500 accessions) is constituted by advanced varieties, the second part of a collection (more than one thousand accessions) – local forms of pumpkins. Not large collection, about 50 accessions, but quite significant for its scientific and breeding values consists of hybrids, self-pollinated lines and donors of valuable traits. As a result of long-term evaluation of breeding accessions the germplasm collection that includes sources of valuable traits has been created. Genetic collection of pumpkin includes accessions with such traits as resistance to a virus mosaic, a naked seeds, bush habitus plants, spaghetti like fruit, parthenocarpy.
24-31 1023
Abstract
The quality of table beet roots is determined by the complex traits and properties. Modern varieties of table beet are mostly heterozygous populations showing the strong variability under the influence of environmental conditions. Ecological and geographical
study of 165 accessions of table beet was carried out and the interrelationship between traits of quality and other important biologically and economically valuable traits such as
cold resistance, early maturity, monogermicity, marketability was studied. The basic traits that characterize the quality of roots of beet, including their color and shape, and also chemical composition, marketability and storability at the long-term storage were described. The data on the inheritance of form and color of beet roots and their chemical composition were given. It was found that the reaction of different varieties and hybrids of beet to change of growing conditions was unequal that showed their genotypic differences. Early maturing varieties of table beet, characterized by the increased cold resistance, as a rule, had intensively colored flesh of roots and good
taste. Relatively stable varietal trait in beet roots is a color. There was no stable relation between the chemical composition, the intensity of the color of flesh and taste. The result of the study of collection accessions showed negative relationship between the sugar content and betanin. It was established that was no significant differences in biochemical composition between monogerm and multigerm varieties of table beet. As 
result of research, the valuable accessions of table beet of home and foreign breeding were revealed and recommended for use into modern breeding programs.
32-38 921
Abstract
Depending on the genotype of monoecious plants Cucurbitaceae family may have different gender expressions: predominantly female, mixed and predominantly
male type of flowering. However, the degree of sexual differentiation can be changed
under the influence of abiotic and endogenous factors. Among the chemicals that affect the level of female flowering in pumpkin crops, preparations based on 2-chloroethylphosphonic acid (Ethephon or Etrelle) are the most promising for hybrid seed production. Study of plant response of squash Cucurbita pepo var. giromontina with varying sex expressions on the treatments with Etrelle revealed common conformities and specificities of preparation action in the condition of Krasnodar region. It is shown the use of treatment once is not effective even if the high concentration range, 500-1100 mg/L, was taken. On gender switch was effectively influenced successive plant treatments with Etrelle at stages of 3-5 true leaves in a wide concentration range from 250 to 700 mg /L., where the restraining was that the start of male flower blossoming was 14-25 days after female flower blossoming. K69 line with predominantly female flowering was more responsive to the variation of concentration and frequency of treatments whereas the line K49 with male flowering was less responsive to the frequency of treatments. It is shown that in the range of effective concentrations, Etrelle may have phytotoxic effects on the growth and development of squash plants at the time of restraining flowering of male flowers. It is important to  ake that into account when choosing a regime of preparation treatments for chemical castration of maternal forms in hybrid seed production of this crop.
39-42 1284
Abstract
Wide use of cucumber hybrids F1 into industry is only possible at the use of lines with female type of flowering as maternal forms. There are difficulties to select such sort of lines and moreover these lines phenotypically may look entirely like female ones often showing the population nature that may be revealed by treatments with growth  substances. Many researchers came to conclusion that gender variation
expressed much in plant ontogenesis and depended on growing conditions. Consequently, to speed up development of female forms, the different growing conditions may be used as provocative background condition. At VNIISSOK in 2013-2015, some self-pollinated female lines with complex of economically valuable traits including resistance to diseases were selected. All these lines under spring greenhouse condition were shown to be entirely female type, belonging to gynoecium
gender type which is characterized by presence only female flowers on the plant. At the same time gender type of the best maternal lines was assessed for economically valuable traits in open field condition. As a result, it was shown that not all female lines, which were entirely female ones in greenhouse condition, also had not male flowers in open field condition. It may be supposed that increase of volume of breeding material being studied and plant growing under harder condition of open field - day and night temperatures variation, lack or abundance of humidity and others - enable to carry out more precise assessment of gender type of maternal forms. According to results of analysis, the open field condition served as provocative background to reveal cucumber lines with strong expression of female flowering. It enabled to have selected eight most promising accessions, which were taken into crossing to test their combining ability.
43-45 1086
Abstract
The standard of organization for model technological process of seed production was
elaborated at VNIISSOK. Requirements established are for implementation of technological operation at radish seed production as transplantation culture as followed: seed plant growing in polyhouses to produce elite seeds, seedlings planting out, plant nursing, harvesting and postharvest processing. The standard is aimed to provide the qualified work in radish seed production through transplantation culture. Radish seed plants for elite seed production are grown on warmed soil beds in winter greenhouses with use of plastic roofing or in plastic houses supplied with heating system. Seeds with germination not less than 85 % according to GOST 32592-2013 are taken for seed production aims. Hand sowing under marker ensures the identical all-around nutrition for plants that is particularly important when growing and selecting the seed plants (1 hectare - 55-60 thousand seed plant roots). Approbation of seed plants is carried out just before harvesting. The terms of seed plant planting are the earliest. Scheme of planting is 70 x 15 - 20 (cm), 60 x 30 (cm) or 90 x 15 (cm). To protect the seed production plantation of radish against weeds, diseases and pests, the pesticides are allowed to apply in accordance with State Catalogue of Pesticides and Agrochemicals, permitted to use in the territory of Russian Federation. Postharvest desiccation of seed plants enables to yield radish by means of direct combining. Radish seeds after processing on sowing qualities have to meet all sowing requirements according to the acting standard. The standard of organization is agreed and affirmed in 2016 CTO
45727225-52-16.
46-51 1007
Abstract
This study was carried out in spring and summer crop rotation in film unheated
greenhouse in condition of Transnistria. The parameters of phenotypic variability were
determined during the continuous periods including: from shoots to plantlet maturing;
from plantlets to flowering; from flowering to ripening F1 hybrids and their parental lines. Early and middle early functionally sterile (gene ps-2) and fertile lines were taken for the experiment. It was shown that duration of vegetative period varied insignificantly in lines and hybrids. Phenotypic variation in interphase periods was also insignificant;
however, the second period was characterized by stronger variation. The lines phenotypic
variation in lines during the continuation of vegetative period and its compounded phases depended on the genotype, year of study and their interactions. On the phenotypic variability of continuation of the period from shoots to flowering sterile lines significantly was caused by the genotype, the variation of the period from flowering to
ripening was equally caused by genotype and year of the study, the variability of the
period from shoots to ripening was affected by genotype. On the phenotypic variation of
the continuation of the vegetation in fertile lines is influenced by the genotype and its
interaction with year of study, on the variability of the period from shoots to flowering
equally genotype and year of study and their interaction on the variability of the period
from to ripening the interaction of genotype and year of study. Inheritance of  continuation of vegetative period and its components, interphase periods, mainly had the character of a negative overdominance (heterosis for earliness). The nature of inheritance of the continuation of vegetation and interphase periods may vary depending on the year of the study; however, there remained its direction. A strong correlation between the continuation of the period from shoots to maturation and its components of the interphase periods. Between the continuations of individual interphase periods from shoots to flowering and from flowering to ripening correlation was insignificant, which indicated the independent nature of inheritance of these periods.

VEGETABLE PRODUCTION

52-53 809
Abstract
Biodegradable ecovio® plastic films for mulching is a solution of BASF engineering.
It offers the excellent mechanical properties, and it is completely destructed
as polymer is to be fully composted. Without recycling this covering plastic film
is entirely digested by soil microorganisms. The only thing is to do after harvesting
is to bury scraps of plastics into the soil to start the process of biodigestion. Development of ecovio® solution opens new page for wide use of this technology in agriculture. The usage of biodegradable plastic films has shown the good results in plant nursery gardens, vegetable and grape growing.
54-61 1111
Abstract
On the base of comparative assessment and average data estimation from 2010 to 2015, the characteristics of 11 cultivars bred at VNIISSOK were described regarding yield capacity and its components, variation, interdependencies at two sowing terms winter,
spring time, direction of selection and cultivar uses at cultivar maintaining breeding program. Cultivar 'Koroleva Margo' is rather used in breeding program for yield capacity and taste qualities. Cultivar 'Sonata' is a source of such features as early maturity, valuable biochemical content and low nitrate accumulation. Cultivar 'Sofit' is known to bear early maturity and simultaneous seed maturation. 'Ariya' is a source of cylindrical root shape and early maturation. Cultivar 'Teplichnyi Gribovskyi' is used in breeding program for early maturation and simultaneous seed maturation. Cultivar 'Feya' bears
resistance to bolting and valuable biochemical substances. Cultivar 'Variant' has such features as root density, ability to grow under low lighting, short-term storability.  Purple-red with white tips is a newmade cultivar 'Mavr' suitable for breeding program for valuable biochemical content, unusual root color, also bearing male sterile forms (MS) for heterosis hybrid breeding. Out of all cultivars regarded, 'Mokhovskiy' is only a genetic source to breed radish forms with edible leaves. The positive weak dependence was revealed between cultivar biochemical characteristics, root weight, dry matter  content, and nitrate content; whereas the positive middle dependence is revealed with sugar contents and negative dependence with ascorbic acid content. According to  iochemical characteristics, the best cultivar population as 'Ariya', 'Sonata' (ascorbic acid); Mavr, Sonata, Variant (dry matter, sugars) have been chosen. Cultivars 'Koroleva Margo', Pheya, Sonata, Sophit, Mokhovskiy are the initial breeding accessions for selection of new cultivar population, MS and MF lines needed for heterosis.
62-63 799
Abstract
Purple horsemint (Monarda citriodora L.) is a perennial plant belonging to Lamiaceae family and being cultivated as aromatic and ornamental crop in many countries of Europe and America. Last years this plant actively gains popularity among gardeners. To elaborate the standard of organization with title 'Seeds of Monarda citriodora L. Sowing qualities. Technical conditions' the basis data has been collected from laboratory of leafy, aromatic, scented and ornamental plant breeding, laboratory of stardatization, rationing and metrology at VNIISSOK. The standard for seeds of Monarda citriodora L.
includes the following sections: field of utilization; normative references; terms, definition, abbreviation, classification; technical requirements; order for receiving and sample piking;
method of control; transportation and storage; security requirements.

НАУКОМЕТРИЯ

64-67 764
Abstract
The article presents data on quantitative and classifying distribution of research workers at
VNIISSOK from 2011 to 2015. The scientific contribution of research divisions in different fields of knowledge was shown according to bibliometric indicators such as publishing activity, citation ratio and Hirsch index.

PLANTS PHYSIOLOGY AND PHYTOCHEMISTRY

68-70 1012
Abstract
Results summarize literature and experimental data on the content of glucose and fructose of different varieties in grapes belonging to different botanical species of Vitis. The ratio of glucose and fructose indicator can be used for fermentation control and prevention of under fermentation in the production of dry wines, as well as an identification parameter to assess the authenticity of grape juice and concentrated
must. The object of the study were grapes of red and white winemaking European and autochthonous varieties, belonging to Vitis, as well as varieties of new selection (Aligote, Albilio, Verdelho, Sersial, Rkatsiteli, White Muscat, Cabernet-Sauvignon, Bastardo of Magarach, Kephesiya, Ekim kara, Golubok). Sugar content in grape samples was in
the range of 180-260 g/l. Total hexoses were determined by HPLC method according to a modified methodology developed by the Department of Chemistry and Biochemistry of Wine of "FSBSI "Magarach ". It was established that the value range of the glucose-fructose index in the grapes cultivated in different viniviticultural regions of the world makes 0.74-1.19. It has been revealed that the glucose-fructose index decreases with the ripening of berries. Low index values are characteristic for the grape that ripens at high temperatures and was cultivated in regions with hot climate. High index values
are characteristic of table grapes and winemaking grape varieties of the species Vitis labrusca, Vitis amurensis and interspecific hybrids. Within the botanical species we can
identify varieties that tend to accumulate higher volumes of either glucose or fructose. These patterns are equally characteristic of white and red grape varieties. The analytical analyzes of the Crimean winemaking grape varieties resulted in the establishment of the glucose-fructose index for the first time, varying within the range of 0.9-1.06.
71-75 808
Abstract
The objective of the research was to study the process of directed fermentation of white
head cabbage variety ‘Slava’, using strains of lactic acid bacteria and their consortium with the degree of their mutual influence. As strains of lactic acid bacteria, we have chosen the following: VCR 536 Lactobacillus casei, Lactobacillus plantarum VKM V-578. To obtain comparable results, all experiments were performed on model mediums. For the first time we studied the dynamics of changes in quality indicators at the process of directed fermentation using strains of lactic acid bacteria (LAB) including their consortiums. The mathematical model developed adequately describes the degree of destruction of glucose and fructose in the fermentation process. The raw material was undergone to homogenization and sterilization with the aim to create optimal conditions
for the development of the target microorganisms and to detect the degree of  restruction of fructose and glucose by different strains of microorganisms. The mathematical model developed adequately described the degree of destruction of fructose and glucose in the treatment process. The use of a consortium of lactic acid bacteria (L. plantarum+L. casei) to this culture medium is shown to be impractical. The addition of fructose in quantity 0.5% to weight of the model medium enabled to intensify significantly the process of white cabbage fermentation.
76-77 811
Abstract
The process of the directed fermentation of cucumber ‘Vodoley’is studied with strains of lactic acid bacteria to improve the final product quality and reducing losses during storage. The objective of our studies was to investigate the dynamics of fructose destruction at the process of directed fermentation of cucumber using strains of lactic acid bacteria (microorganisms) and their choosing for this process. The following strains of lactic bacteria (LAB) were used: Lactobacillus brevis In the VCR-1309, VCR 536 Lactobacillus casei, Lactobacillus plantarum VKM V-578. To obtain comparable results, all experiments were performed on simulated medium. The raw material was homogenized and sterilized to make optimal condition for development of the target microorganism. The different strains of microorganisms were tested to detect the  degree of fructose destruction. The developed mathematical model adequately described the degree of fructose destruction at the process of treatment. Mathematical data processing on fructose destruction at the process of directed fermentation was performed using Microsoft Excel and SYSTAT TableCurve 2D. It is established that use of strain L. brevis was the most effective providing maximal efficiency according to the  criterion of intensity of fructose destruction at process of fermentation in cucumber where the maximal acceptable duration is 11-14 days to reach 92% of fructose destruction to asymptotic value. The strain L. brevis enabled to achieve the highest degree of fructose destruction, which, has presumably indicated the potential feasibility of using these lactic acid microorganisms.
79-83 1088
Abstract
At present there is numerous evidence of the antioxidant positive role in the defensive reaction that is capable to protect not only plants, but also humans against oxidative stress. Plant pigments such as natural dyes from leaves, flowers and fruits are known to have high antioxidant activity. Amaranth species A. tricolor L. cultivar ‘Early Splendor’ is a convenient model for the comparative studying of the formation processes of differently colored pigment composition in leaf tissues that differs in the ability to photosynthesize. Leaves of amaranth cultivar ‘Valentina’ were as a standard. The aim of the experiment was a comparative studying of the pigments content: amaranthine, chlorophyll a and b, carotenoids in the cauline leaves of amaranth cultivars ‘Valentina’ and ‘Early Splendor’, as well as in the red and green areas of the leaves. Analysis of the aqueous extract of red Early Splendor amaranth apical leaves showed the presence of betacyanin pigment - amaranthine, in the absorption spectrum in which peak was seen in the green region at 540 nm. In addition to the antioxidant amaranthine there are  also antioxidants which might be phenolic glycosides, and ascorbic acid in the extract, the total content of which is almost twice as small as in the leaves of amaranth cauline of this cultivar. Yellow fraction was found in the ethanolic extract of red leaves. Its absorption spectrum had peaks in the blue region at 445 nm and 472 nm and a shoulder at 422 nm that indicated the presence of betaxanthin, betalamic acid or
carotenoids. Water-soluble antioxidants - amaranthine and ascorbic acid were found in  auline leaves of studied species. Their content in the leaves of Valentina cultivar was higher than in the leaves of cultivar ‘Early Splendor’, and the maximum level of photosynthetic pigments was found in ‘Early Splendor’ leaves. The obtained results showed that the amaranth is a promising source of pigments with the antioxidant  activity that can be used for production of food dyes.

PLANT PROTECTION

84-91 883
Abstract
Selection of infected material for research was carried out in experimental carrot plots at GNU VNIIO (Moscow region), Voronezh OOS (Voronezh oblast), Baraccudas OOS (Rostov
region) and Israel. The first year plants of carrot, the roots, were used for the study. There are many scientific papers cited where many different methods of artificial infection of plants with fungal disease pathogens were given to control the stability of carrot genotypes in various phases of plant development. One of the ways to lead the purposed breeding program for resistance is the extraction of pathogens isolates and the method of fast assessment on the basis of determining the aggressiveness of new strains and their use in breeding work. This article presents a method of inoculation of cut-discs of root. A great advantage of the method is in its efficiency and the current results can be obtained within 2 weeks from the moment of infection. As a result, the work on the isolation of fungi from carrot plants, the pure culture collection of strains: Alternaria radicina and Fusarium avenaceum was obtained. The characteristics and
morphology description of colonies of isolates of RR. Alternaria and Fusarium found in carrot plants taken from different ecological and geographical zones were given. The most aggressive isolates of the following ecological and geographical zones as Alternaria at Moscow and Rostov regions, Fusarium at Voronezh and Moscow regions were revealed. These isolates will be used as standards of aggressiveness for the test of newly isolated strains regarded as inoculum to conduct immunological experiments
in carrot breeding for resistance to Fusarium and Alternaria.

AGRICULTURALMANAGEMENT

92-97 1162
Abstract
The goal of the present paper is to identify the optimal strategy of development of the Russian olericulture in order to substitute imported products and to build up logistic and transport infrastructure. Existing problems of the Russian olericulture are described. It is demonstrated that these problems can be solved on the basis of big integrated structures. Formation of these structures can be based on hierarchical (vertical  integration) or networking (agricultural filieres or clusters) models. A comparative analysis of these models of development of olericulture is made. Advantages and inconveniences of each model are described. It is demonstrated that sustainable development of the Russian olericulture can be insured only by a combination of hierarchical and networking tools. Vertical integration will help to reach quick increase of production, while networking models are necessary for inclusion of small producers into production chains, development of product range and development of supporting industries. Networking models are also necessary for social tasks. It means that the optimal strategy of development of the  Russian olericulture should be based on a combination of networking and hierarchical tools. This combination is necessary for agricultural corporation as well as for the Russian olericulture in general.


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ISSN 2072-9146 (Print)
ISSN 2618-7132 (Online)