BREEDING, SEED PRODUCTION AND PLANT BIOTECHNOLOGY
The article reflects the issues of the current state of scientific research in the FSBSI FSVC, directions and prospects for the development of selection and seed production of vegetable crops are shown. Varieties have been created that realize the bioclimatic potential of the cultivation zone, meet market requirements, and are capable of producing high yields under the influence of biotic and abiotic stressors. Selection is carried out in a combination of classical methods with innovative technologies.
Biotechnologists have improved the basic protocol for in vitro microspore culture for the Brassicaceae, stages of technology for obtaining doubled haploids in microspore culture in vitro for the Apiaceae are being developed. For the first time, an effective protocol for obtaining double haploids of European radish has been developed. Doubled haploids of vegetable crops of the Cucurbitaceae, Amarantháceae, Amaryllidáceae were obtained in vitro in the culture of unpollinated ovules. The Center conducts research on the increased content of biologically active substances and antioxidants. An important place is occupied by immunological studies on the resistance of vegetable crops to harmful diseases. The institution develops systems of protective measures against pests, diseases and weeds for vegetable crops based on the use of new environmentally friendly preparations. On the basis of fundamental and priority applied research of previous years, the creation of 21 varieties and hybrids of cabbage, table beet, tomato, sweet pepper, onion, chives, leek, cucumber, melon, lettuce, chervil, potato, aster, lychnis has been completed. Along with the creation of new breeding achievements, technologies for their cultivation are being improved through the use of new agricultural practices, microfertilizers, biological products and humates on vegetable crops in various soil and climatic zones of Russia. New agricultural methods are being developed in the cultivation of mushrooms. In the field of seed production, methods are being developed to increase the germination of seeds, seed production, elements of the technology for the production of mother liquors and seeds of various vegetable and melon crops; zonal seed production technologies that increase their yield. In 2021, a team of scientists received an award from the Government of the Russian Federation for scientific work "Development and implementation of innovative technologies for growing vegetables and potatoes to provide the population with environmentally friendly food".
Vegetable peas are by far the most widely used among the main vegetable legumes. Due to its high nutritional value, it has an important food value and is cultivated almost everywhere. High nutritional qualities of vegetable peas are determined by the content of protein, carbohydrates, dietary fiber, vitamins, as well as macro– and microelements. Pea protein is popular due to its affordable price compared to animal protein. The value of pea protein is determined by its amino acid composition and its high balance, especially valuable amino acids that are not synthesized in animals and humans.The article discusses the nutritional value of vegetable peas (green beans; fresh, frozen and canned vegetable peas; dry seeds); the content of water-soluble protein, amino acid composition, the content of macro- and microelements in the seeds of vegetable peas of FSBSI FSVC selection varieties; the quality of green peas as raw materials for canning, depending on the type of seeds and the structure of starch grains; requirements for the quality of raw vegetable peas for canning; seedlings and microgreens as useful and nutritious products for fresh consumption; medicinal properties of vegetable peas; varieties of vegetable peas for various uses.
Introduction: Anthocyanins, the polyphenolic plant pigments, have high antioxidant activity (AOA), reduce the risks of many pathological conditions in the human body. However, the wide medical and preventive use of anthocyanins is limited by their degradation during processing of plant raw materials. The objective of the work was to study the anthocyanins’ stability by spectrophotometric method during heat treatment and storage of vegetable and berry plants.
Study objects and methods: Purple potatoes, eggplants, red cabbage, purple carrots, blue onions, red raspberries and blue honeysuckle were used in the study. Anthocyanins’ stability was determined by numerical values of extracts’ absorption spectra from the studied plants.
Results and discussion: High stability indices of 0.623–0.986 were obtained for the anthocyanins of purple carrots, blue onion bulbs or red cabbage leaves whose main component is the antioxidant cyanidin with a relative AOA equal to 3.49. Low stability indices of 0.229-0.23 were obtained for anthocyanins of red raspberry berries and purple potato tubers containing pelargonidin or malvidin with 2.49–3.36 times lower relative AOA than for cyanidin. A regular correlation between stability and AOA of anthocyanins with a rank correlation coefficient of 0.91 (p<0.05) was established.
It was established that during three months of storage in a domestic refrigerator, the content of anthocyanins in purple carrots and purple potatoes decreased by 10–15% of the initial one, and this decrease was 2-3 times greater when other studied plants were stored for 1.5–2 months.
Conclusion: It is advisable to use the developed spectrophotometric methods in the express-analysis at selection of perspective plants for industrial cultivation as raw material for anthocyanin-containing herbal formulation.
The priority direction in melon breeding is the creation of new competitively capable varieties that combine resistance to adverse environmental factors with valuable economic characteristics.
Materials and methods. The object of the study is melon varieties 599 and 595. The purpose of this study is to study the influence of weather conditions on the yield and biochemical composition of the studied melon varieties in the conditions of the Volgograd Volga region. The research was carried out at the Bykovskaya melon breeding experimental station for three years according to the developed methods, the Osen variety was used as a standard.
Results. The article presents the results of the influence of weather conditions on yield, fruit weight, and also presents an analysis of the biochemical composition of melon fruits. A complete description of the studied melon varieties is given. In terms of yield, the high indicators for cultivars 599 and 595 were in 2020 with the totals of average monthly active temperatures of 114°C and precipitation totals of 178 mm, and amounted to 19.0 t/ha and 21.3 t/ha. According to the content of dry matter in the fruit juice, according to the results of studies, it was seen that with the greatest amount of moisture, the indicators were higher in 2018 with a precipitation rate of 296 mm in cultivars 599 – 13.6%, in 595 – 13.2%, than in 2019 with a norm of 272.6 mm 599 – 12.0% in 595 – 11.6%. Thus, we can conclude that these melon varieties meet the modern requirements of the industrial melon industry.
HORTICULTURE, VEGETABLE PRODUCTION, VITICULTURE AND MEDICINAL CROPS
Water is one of the main factors for the production of vegetable crops due to its crucial role in the absorption and transport of nutrients, temperature regulation and some physiological processes, including photosynthesis. Considering the growing needs of a growing population for food and nutrients, a significant part of agricultural research is focused on improving the use efficiency (WUE - Water use efficiency) and saving water without reducing yields. Given the difficulty of increasing WUE through selection due to the tradeoff between photosynthesis and transpiration, agronomic strategies are needed. Due to the shallow root system and the fresh sale of vegetable products, vegetable crops are relatively more sensitive to moisture than field crops. Deficit irrigation (DI) is a direct approach to saving water by reducing irrigation to increase water productivity (WP - Water productivity). Regulated deficit irrigation (RDI) and partial root drying (PRD) are two widely used DI planning methods along with the classic DI approach. We searched the peer-reviewed literature for studies reporting yield differences in vegetable crops subjected to irrigation deficits. The search was conducted on Google Scholar and Web of Science using various combinations of the following keywords: vegetable crop yield and irrigation deficit or moisture shortage or water shortage or drought. Moderate levels of water stress (<65% FI) adversely affect vegetable yields regardless of external factors such as soil structure, climate and production system. This level of irrigation is justified for regions where water restrictions are already in place or forecast. Based on WP growth, irrigation levels as low as 35–50% FI for tomato and pepper, and 50–65% FI for onions and <35% FI for eggplant and watermelon are optimal for areas with severe water scarcity. However, a higher water deficit may also have an adverse effect on quality in terms of reduced fruit/root/bulb size.
Relevance. Currently, there is an acute problem of meeting the growing demand for berry and fruit products. One of the ways to intensify agricultural production in the field of horticulture and crop production is to increase the efficiency of environmental management through the use of resource-saving technologies. One of these technologies is drip irrigation, which improves the quality of crop production.
Materials and methods. Field studies were carried out on the territory of the educational and experimental farm of the laboratory "Michurinsky Garden" of the Russian State Agrarian University – Moscow Timiryazev Agricultural Academy. The experiment was established in the fall of 2018 and is a two-factor study of various levels of moisture on the growth and development of raspberry seedlings. The first factor included options for maintaining soil moisture in the range: 1) control (without irrigation); 2) not less than 60% of the lowest moisture capacity; 3) not less than 70% of the lowest moisture capacity; 4) not less than 80% of the lowest moisture capacity. The second factor was the raspberry varieties Solnyshko and Nagrada.
Results. Constructed moisture contours according to the study options showed that drip irrigation contributes to the optimum moisture concentration in the soil for seedlings. The most developed root system in comparison with the control was obtained on irrigated variants with maintaining a moisture content of at least 70 and 80% of the lowest moisture capacity. Here, the maximum values of the volume of the root system, the number of roots, and the average length of the root were obtained. It was revealed that in variants with irrigation, the root system of seedlings spreads in the upper layer (mainly 5-15 cm).
Common chicory (Cichorium intybus L.) is widely known in traditional medicine of many countries, but in official medicine its use is limited as a part of biologically active additives. Despite the available information about pharmacological activity of leaf extracts, their use as a medicinal herbal raw material is almost not considered. The purpose of the work is to study the accumulation dynamics of phenolic compounds (PC) in the leaves of cultivated chicory to optimize the time of harvesting the above-ground mass. The article presents data on the accumulation dynamics of PC in the leaves of two varieties of cultivated chicory, and also compares the obtained data with similars of wild-growing chicory.
Results. It was noted that the content of phenolic compounds in the leaves of cultivated chicory is 1,5-2 times higher in comparison with the raw materials of wild plants. The studied varieties differ in the content of PC by 8% in average. It has been established that the main phenolic compounds in the leaves of cultivated chicory are chicoric, chlorogenic and cafftaric acids; their total content in the obtained extracts is 55 %. It was noted that the period of maximum accumulation of PC in leaves falls on the end of september – beginning of october. The established chemical composition, a higher content of phenolic compounds compared to wild plants, as well as the coincidence of the maximum accumulation of PC in the leaves with the approximate date of harvesting chicory roots allows us to consider its cultivation in culture as a promising direction with the aim of the integrated use of the obtained raw materials for both food and pharmaceutical industry.
The aim of the study was to determine the growth, development and yield of sweet corn varieties and hybrids as vegetables during the soil-climatic conditions of Karakalpakstan.
Methods. The 8 cultivars and 8 hybrids studied were evaluated for growth, development and yield in Karakalpakstan in 2017-2019. Selection work was carried out on varieties and hybrids. Varieties and hybrids with a positive result based on the selection results were recommended for use in subsequent experiments.
Results. After sowing the seeds of the studied 8 varieties and 8 hybrids of vegetable (sweet) corn, the duration of the germination period was 7-9 days. Relatively early germination between cultivars and hybrids Zamin, Can. Pedro 2 Inta, Berys varieties, relatively late germination was recorded in 9 days in SPV 1022, Leonard's Early, Honey Bontam F1, Spirit F1, Megaton F1, Baron F1, Soyan F1, Hybrid F1. It has been established that the period from germination to the stage of leaf formation (7 leaves) is 13-16 days, the period from the stage of leaf formation (7 leaves)to the panicle formation is 23-33 days, the period from the panicle formation to flowering is 4-6 days, the period from flowering to the cob formation is 9-15 days. In the studied varieties and hybrids, the period from the cob formation to milky ripeness was 13-18 days, from milky ripeness to waxy ripeness lasted up to 4-8 days.The study of the duration of the development phases of varieties and hybrids showed that the growth period from germination to milky-wax ripeness is 75-85 days. Early yield was observed in hybrids Baron F1, Sweet star F1, Spirit F1, Soyan F1, vegetation period 73-77 days, relatively early yield was observed in varieties and hybrids Zamin, Sentinel F1, SPV 1022, Osnova 209, Hybrid F1, Can Pedro 2 Inta, Clx3349ys clause, growing season 79-81 days.The selection of varieties and hybrids most appropriate to the soil and climatic conditions of Karakalpakstan was carried out and recommended for cultivation by farmers and dekhkan farms.
AGROCHEMISTRY, SOIL SCIENCE, PLANT PROTECTION AND QUARANTINE
Relevance. The 28-spotted potato ladybird beetle, Henosepilachna vigintioctomaculata, causes severe damage to plants of the Solanaceae family in the south of the Russian Far East. Today the application of chemicals is the main method for protecting crops against the potato ladybird beetle. This leads not only to the eradication of the pest, but also to the pollution of agricultural ecosystems and the emergence of potato ladybird beetle populations that are resistant to pesticides. A study on the seasonal cycles of the development of the potato ladybird beetle may help to devise new methods for controlling this pest.
Methods. We conducted laboratory experiments to study the developmental timing ofa potato ladybird beetlepopulation. The number of eggs was counted, and then the eggs were placed in Petri dishes. The number of emerged larvae was recorded on a daily basis. The hatched larvae were transferred to glass containers (hereafter rearing cages) in batches of 10. We recorded the dates of the transition from one immature developmental stage to another notingthe simultaneity of these transitions. At the onset of the pupal stage, the date was recorded and food was withdrawn from the rearing cages. Scientific observations were carried out on the emergence ofyoung beetles. Field research on the phenology of the potato ladybird beetle was conducted at afield site of 40 m2. The timing of the following events was recorded: the emergence of the adult beetles from diapause, the colonization of the potato field, the beginning and the end of oviposition, the emergence of the larvae and the pupae, the flight of the new insect generation.
Results and conclusion. Our laboratory experiment on the immature developmental stages of the potato ladybird beetle revealed that the egg stage was 4-5 days in duration, the larval stage was 16-17 days and the pupal stage was 4-5 daysunder optimal conditions. We also observed deviations from the mean values, which could be conditioned by external factors. For instance, the duration of embryonic development depended either on humidity or on the time range of hatching from one egg mass. The observed deviations of the developmental timing of the larvae and the pupae were most probably due to the quantity and quality of the available food, and the presence of secondary metabolites and glycoalkaloids in it. The field research on thephenology of the potato ladybird beetle showed that there was only one generation in 2020, but two generations in 2021. After comparing climatic conditions in 2020 and 2021, we concluded that Henosepilachna vigintioctomaculata can produce two generations during dry and hot years.
AGRICULTURE AND PLANT PRODUCTION
Relevance. The use of non-invasive photometric methods for assessing the stress states of plants makes it possible to assess the physiological state and resistance of plants.
Material and methods. In small-field and vegetation experiments, we studied the photometric indicators of the physiological response of potato varieties (reflection coefficients in different ranges) to nitrogen stress created by five doses of fertilizers using contact (N-tester) and remote (N-sensor) methods.
Results. Potato varieties released for the conditions of the North-Western region of the Russian Federation were studied for their ability to withstand stress provided by low and high levels of nitrogen nutrition of plants and different weights of planting tubers. The resistance of plants of different genotypes to external and internal stressors was studied by the reflection coefficients measured in 4 channels, which characterized the differences in the physiological state of plants. Ensuring the potential genotypic resistance of the organism in the form of a decrease in FIS F1,3 corresponds to the inclusion of various mechanisms of plant adaptation to external or internal influences, depending on the genotype. In the vegetative experiment, of the studied photo-optical reflection coefficients, the most informative were the FIS F1,3 indicators, which showed that the overall viability of such varieties as Peterburgsky, Charodey and Kholmogorsky turned out to be increased. The reaction type of the Kholmogorsky variety is the most promising for growing plants under stress conditions.
Relevance. Currently, there is an increasing interest in nutritional supplements of natural origin. One of the crops that can be used for this is sweet potato. The inclusion of flour from dried sweet potato tubers with colored pulp in the recipe of wheat bread can contribute to its enrichment with vitamins and antioxidants. Therefore, the study of the suitability of sweet potato tubers obtained in the conditions of the Non-Chernozem Zone for use as an additive in the production of bread is an urgent task.
Material and methods. The article presents the results of a comparative study of the appearance and taste of bread with the addition of various amounts of powder from root tubers with colored pulp of two varieties of sweet potato (Purple 1 - anthocyanin and Beauregard – carotenoids).
Results. It has been established that sweet potato root tubers grown in the conditions of the Central region of the Non-Chernozem zone are suitable for use as a natural food additive to wheat bread. The positive effect of preliminary steaming of tubers before drying on the volumetric yield of bread and the color of its crumb was revealed. The studied varieties of sweet potato differed significantly in the yield of air-dry powder - the Purple 1 variety had an advantage over the Beauregard variety. Bread with additions of 5% and 10% of Purple 1 tuber powder was characterized by the best appearance and taste. The Beauregard variety had a high sugar content, which negatively affected the consumer advantages of bread with its additives.
The results of evaluating the effectiveness of the plant growth regulator Atonic Plus on potatoes as a stimulator of development, immunomodulator and protectant of the negative effects of pesticides in the crop protection system in the agro-climatic conditions of the Central region of the Russian Federation (Moscow region) are presented.In the extreme weather conditions of the growing season of 2010, the growth regulator Atonic Plus effectively demonstrated its qualities in the form of the formation of adaptability and anti-stress resistance in potato plants, providing an increase in yield relative to control by 1.8-7.1% and the quality of tubers is not only compared to the control, but also relative to the reference version. The combined use of a growth regulator with a fungicide contributed to an increase in the yield of standard potatoes in early and mid-late potato varieties by 2-2.5 times compared with the control.
Relevance. The results of a study on the use of organomineral fertilizers obtained using biocatalytic processes for detoxification of poultry waste and their influence on the growth and development of plants during the growing season are presented.
Methodology. For poultry farms, it is necessary to create and introduce waste-free technologies for the preparation of fertilizers. To increase the efficiency of the fertilizer as a biological catalyst, we used a bacterial growth stimulator sodium humate, obtained by us according to a previously developed technology from brown coal from the Maikubenskoye deposit in the Republic of Kazakhstan («Method for producing sodium humate» Patent No. 4600 RK), as well as a sorbent based on sodium humate (a by-product of obtaining sodium humate), which was used to detoxify the feedstock and the resulting product from heavy metals and their salts due to its absorption-adsorption and complexing properties. For the developed technology for producing organic fertilizers, an application has been submitted for obtaining a Patent for the invention of the Republic of Kazakhstan «Method for producing organic fertilizers» (priority No. 2021-22818, dated July 13, 2021). Studies to determine the effectiveness of the resulting fertilizer were carried out on the experimental plots of the peasant farm «Flame», Pavlodar district of Pavlodar region of the Republic of Kazakhstan. In the soil intended for growing seedlings of tomatoes of the «Pepper-shaped Orange» variety and peppers of the «Bogatyr» variety, the resulting fertilizer was applied in an amount of 1 kg per 1 m2 of soil, which was dug to a depth of 8-10 cm and used for growing seedlings in greenhouses and in subsequent for planting it in open ground. xperimental and control studies were carried out under equivalent climatic conditions, scheme and technology of watering plants. In total, 100 bushes of each plant species were used in the experiment.
Results. The use of the obtained organomineral fertilizer makes it possible to increase the yield of vegetable crops (tomatoes, peppers) by 20-25% in comparison with the control. In addition, in the experimental samples, an increase in the number of fruits on the bush was noted with an increase in their size, a decrease in the growing season and the number of damaged fruits.
IRRIGATION ENGINEERING, WATER MANAGEMENT AND AGROPHYSICS
The article is devoted to the principles of optimizing the composition of the complex of machines for cleaning the reclamation channels of the drainage network The fundamental initial documents in determining the composition of the complex of machines for the production of operational, repair and restoration works are technological maps for carrying out repair measures to restore the reclamation channels of the drainage system. Technological maps are formed taking into account the requirements for the elements of the drainage system, due to the specific conditions of the zone of economic regions, the natural and production characteristics of reclamation facilities.
ISSN 2618-7132 (Online)