BREEDING, SEED PRODUCTION AND PLANT BIOTECHNOLOGY
Relevance. The use of modern biotechnological methods in breeding, namely the doubled haploid technology and marker-assisted selection, can significantly reduce the time for creating pure lines with valuable properties. Fusarium wilt is one of the economically important diseases of white cabbage. Therefore, the development of a MAS method for selecting doubled haploids resistant to fusarium wilt would allow fast selection of resistant pure lines. Moreover, the resistance testing can be done at any plant developmental stage.
Materials and methods. The response of hybrids Gertsoginya F1, Poisk 2018 F1 (resistant samples, the originator – the Agrofirm “Poisk”) and Slava 1305 (susceptible sample, the originator – Federal Scientific Vegetable Center) towards Fusarium oxysporum f. sp. conglutinans infection was determined by evaluating their growth on the inoculated soil. To test the efficiency of the FocBNUf/r marker to the polymorphic region of the FocBo1 gene, the markers were tested on resistant and susceptible genotypes. Then the markers were applied to analyze 60 doubled haploids of 12 different genotypes.
Results. It was demonstrated that the FocBNUf/r marker effectively differentiated resistant and susceptible samples. Doubled haploids were obtained from 12 different breeding samples for FocBNUf/r marker-based selection of fusarium wilt resistant plants. PCR testing of doubled haploids for fusarium resistance allowed us to select 6.7-100% of plants with the fusarium resistance gene in a homozygous state in 8 genotypes. 4 genotypes of doubled haploids that did not carry the resistance gene were eliminated from the breeding program.
Conclusion. The FocBNUf/r marker effectively identified resistant and susceptible samples, as well as differentiates homozygous and heterozygous plants. FocBNUf/r marker was used to select resistant doubled haploids of white cabbage at the seedling stage. Thus, it was possible not only to accelerate the production of pure lines by obtaining doubled haploids, but also to accelerate the selection of valuable samples carrying the gene of resistance to fusarium, which makes it possible to avoid the labor-intensive selection of resistant lines on inoculated soil.
Relevance. Xanthomonas campestris pv. campestrisis a widespread bacterial pathogen that causes a black rot disease of Brassicaceae plants. It is distributed across all territories with cultivated brassica crops and has high economic significance. One of the known Xanthomonas pathogenicity factor is TAL-effectors. These proteins penetrate in plant nucleus and change expression of some host genes in order to promote infection. For some Xanthomonas species TAL-effectors are a key of bacteria success, however for Xcc their role is still remain obscure as well as TALE occurrence among Xcc belonged to different races and originated from different regions. The goal of our study was examination of TALE distribution among Xcc isolates collected in Russia.
Methods. Sample of the Xcc isolates mainly collected in Moscow region and Krasnodar krai was used for TALE search. Bacterial total DNA was isolated using CTAB method. Four primer pairs were used for TALE genes detection by amplification conservative regions.
Results. Among 50 isolates only 4 isolates possessed TALE were revealed. Low-frequency occurrence of TALE among Russian isolates can reflect Xcc population features in Moscow region and Krasnodar krai as well as genotype features of cultivated plant hosts.
Relevance. The trend of increasing the area of protected soil and introducing new technologies in the cultivation of vegetable crops of food value can be traced every year. With the development of technology, the requirements for the quality and volume of seed material of hybrids have increased. The main constraints on seed production are the requirements for additional manual labor costs and the complexity of the process, in particular, pollination elements that need to be studied and improved.
The aim studying some elements of technology, the optimal time between castration and pollination when crossing fertile tomato lines.
Material and Methods. The research was conducted in 2022 year in greenhouse at the Federal State Budgetary Institution "Federal Scientific Vegetable Center". The objects of research were different methods of applying paternal pollen to the stigma of the maternal form. The experiment was carried out between maternal and paternal mid-fruited semi-determinate tomato lines in a hybrid combination. The variants of our experience include time-varying methods of pollen application. Pollination at the same time after castration is a control option, pollination after 4, 12, 24, 48 hours. Options with additional pollination the next day: pollination after 4 (+ after 24 hours), 12 (+ after 24 hours), 24 (+ after 24 hours), 48 hours (+ after 24 hours).
Results. In the research, it was found that when pollinated with pollen of the paternal component 24 hours after castration of flowers with additional pollination the next day (24 hours later), the yield of seeds from one fruit on the average fertile maternal line of tomato exceeds 3 times compared with conventional pollination at the same time with castration.
Relevance. The selection for productivity and stability on different stages in the breeding process includes an assessment of the adaptive capacity and genotypes ecological stability. Therefore breeding for the resistance to abiotic environmental factors is closely connected with the adaptive breeding, which has received special attention in recent years.
Methodology. The work was carried out at the “A.L. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar” in 2011-2020. Male-sterile forms of sugar beet were crossed with fertile diploid multi-seeded pollinators (ARRISBS selections) by topcross method. The resulting hybrids were assessed for yield, sugar content and sugar collection according by standard methods used at the “A.L. Mazlumov ARRISBS”. It is carried out the annual work, including selection of the best and rejection of the worst options for the studied characteristics. An assessment was made of lines and hybrid combinations of sugar beet for the resistance to a complex of unfavorable natural and climatic factors based on the characteristics of yield, sugar content and seed productivity.
Results. The results of improving selections over ten years show an increase in the weight of the root crop and the sugar content for the original single-seeded and multi-seeded materials. Single-seeded (MS-2113, MS-Perla) and multi-seeded (OP-15465, OP-15676, OP-15202) lines with high combining ability in terms of yield and sugar content of root crops were identified. An increase in the resulting parameter - sugar collection - was noted by 2020 (to 8,8-9,0 t/ha), illustrating the positive dynamics of the breeding process of sugar beet for plants of the first life year. An increase in the weight of 1000 seeds (to 13,4-13,9 g) and separate fruiting (to 98-99%) was shown during the breeding process for plants of the second life year.
Conclusion. The studied hybrid combinations have a wide reaction rate to a complex of natural climatic factors: elevated temperatures in combination with high and low humidity.
Relevance. Onion (Allium cepa L.) is a valuable and demanded vegetable crop, which occupies one of the leading places in terms of sown areas and gross yields among other vegetable crops of the genus Allium L. Onion is popular in folk and evidence-based medicine, widely used for the prevention and treatment of many diseases due to its rich biochemical composition and useful properties. As part of import substitution and achieving food security of the country, scientists of the Federal State Budgetary Scientific Institution «Federal Scientific Vegetable Center» (FSBSI FSVC) are working intensively to create new generation varieties and hybrids.
Methodology. Various resources on the topic were selected, publications in scientific and industry journals and databases were studied, a review of works on onion selection (yield, species diversity in shape and color, maturity, shelf life, biochemical parameters, commercial and seed productivity, resistance to biotic and abiotic stresses), as well as useful properties for humans and use in medicine was made.
Results. The analysis of works shows that the wide distribution in the Russian Federation have such varieties of selection of FSBSI FSVC, such as Myachkovskij 300, Cherni prince, Al'ba, Atas, Ampeks and others. In recent years, created a medium-maturing hybrid onion onion F1 Drakon with brown dry scales and a maximum yield of 391.0 c/ha, as well as transferred to the State variety trial winter variety Novator with brown dry scales. Propagation of demanded varieties and hybrids for both production cultivation and for use in household farms is underway.
Conclusion. In order to conveyor onion production, it is necessary to create varieties and hybrids of different maturity groups, as well as to work on improving marketability, seed productivity and resistance to various stresses.
HORTICULTURE, VEGETABLE PRODUCTION, VITICULTURE AND MEDICINAL CROPS
Relevance. In the modern production of greenhouse products, with hydroponic cultivation of crops, one of the safe ways to increase the quantity, quality of cucumber products and plant protection is the use of microbiological preparations.
Methodology. The purpose of our work was to assess the effect of a complex of microbiological preparations on the productivity of cucumber hybrids in winter and spring turnover. The study was conducted in industrial greenhouses during 2021-2022. Long-fruited (Lohengrin F1) medium-fruited (Mewa F1 and Svyatogor F1) and short-fruited (Valigora F1) parthenocarpic cucumber hybrids were selected as research objects. The preparations were applied five times, at 30-day intervals throughout the entire growing period, the first application when planting plants in the production department. Measurements of economically valuable and chemical indicators were carried out weekly. Descriptive statistics and ANOVA methods were used for statistical data analysis.
Results. As a result of the research, a positive stimulating effect of a complex of microbiological preparations on the accumulation of raw and dry biomass, an increase in yield and its quality in the studied cucumber hybrids was revealed. The final yield when applying microbiological preparations increased on average from 4.1 to 5.5 kg/m2 in all cucumber hybrids over the two years of study, while the yield of standard products in the total crop structure increased by 2.7% - 6.4%.
Relevance. Crop productivity is determined by genotype, environmental effects and their interactions. Comprehensive evaluation of crop varieties based on their adaptability, plasticity and stability makes it possible to select the most promising varieties, high-yielding and adapted to many environmental conditions to improve productivity.
Materials and Methods. Fourteen common bean varieties were tested in a randomized complete block design with three replications during two years (2022 and 2023) in the experimental field “Michurintsev Garden” of Novosibirsk State Agrarian University. Productivity traits were taken into account: number and weight of beans per plant, weight of 1000 seeds, yield. The purpose of the work was to evaluate bean samples for adaptive capacity and ecological stability in the conditions of the forest-steppe Priobie.
Results. The article provides an assessment of the adaptability and stability of bean varieties in order to identify varieties with high productivity and adapted to climatic and farming conditions in the Siberian region. The results of the tests showed that the highest average net yields of Rubin, Krasnopestraya, Zolotistaya and Muhranula varieties were in the range of 0.41-0.64 kg/m2. Analysis of genotype-environment interaction (GxE) according to the mathematical model of Eberhart and Russel shows that varieties Rubin, Krasnopestraya, Zolotistaya and Muhranula have high indices of general adaptability and breeding value of genotype, Among them, varieties Rubin and Zolotistaya have stable productivity and high adaptability to environmental conditions.
Relevance. To increase the yield of aubergine, a number of agrotechnical methods have been studied. In the conditions of the Astrakhan region, one of the methods is the cultivation of aubergine without seedlings, in which it is very important to select varieties adapted to these conditions, suitable for the duration of the growing season. Cultivation of aubergine without seedling method, in comparison with seedling method, allows to exclude material and technical costs for growing seedlings. To increase the productivity of selected varieties, an important agronomic technique is the use of plant growth regulators that increase plant resistance to unfavourable environmental factors and have a stimulating effect on plant growth and development.
Material and methodology. In conditions of Astrakhan region on alluvial-meadow, medium-loamy soils with granulometric composition were carried out studies to identify varieties that are best adapted to these natural-climatic conditions and give the highest yields in seedless cultivation. In the second experiment on seedless aubergine the effect of growth regulators on plant productivity was studied using preparations - Melafen, BP, Gibbersib, P, Biodux, Zh, Epin extra, R.
Results. On drip irrigation in conditions of Astrakhan region under cultivation by seedless method were studied varietal characteristics of 6 varieties of aubergine with fruits of cylindrical shape, fruit index 4,3-4,4. Among the varieties studied, Almaz and Alekseevsky stood out by the main productivity indicators, forming 6.2-6.6 pieces of fruit per plant with an average weight of 220.7-223.5 g, which provided the formation of yield 42.3-45.8 tonnes/ha. The use of plant growth regulators Melafen, BP and Epin Extra, P on the variety Alexeevsky at the seedless method of cultivation promoted the friendly appearance of shoots, activated growth processes; the number of lateral shoots increased by 1.3-1.5 pieces, the raw above-ground weight of plants was greater by 29.1-34.0 g, the total yield increased by 27.1-34.5%, compared with the control. The varieties most suitable for growing by seedless method and showing the highest yield were identified - Alekseevsky, Almaz. It was found that the use of growth regulators accelerated plant development, increased the height of the main stem and the number of lateral shoots, weight and number of fruits on the plant, which was reflected in plant productivity. Application of growth regulator Epin extra, P allowed to obtain a yield of 52.2 t/ha, which was 34.5% higher than the yield of the control.
Relevance. Improving zonal agricultural technologies for the cultivation and seed production of vegetable crops is due to the need to increase agroeconomic and technological efficiency, production profitability and competitiveness of domestic products in a market economy and Western sanctions in order to ensure the country's food security and import substitution.
Methodology. Improving the elements of zonal agricultural technology for cultivating the new variety of sweet pepper Zarnitsa to increase the productivity and quality of seeds and reduce the costs of its seed production in a seedless culture through the use of micro fertilizers (Ultramag Boron) and plant growth regulators (Zircon R) is the goal of the ongoing research.
The objective of the research is to study and evaluate the agroeconomic efficiency of using the Ultramag Bor micro fertilizer and the Zircon, P plant growth regulator and their impact on the dynamics of plant growth and development, ripening time, yield and quality of sweet pepper seeds in the experiment.
The practical significance of the research lies in the possibility of widespread use in the real sector of the economy of plant growth regulators and micro fertilizers in non-seedling seed production of sweet pepper to improve productivity, quality and economic indicators of its cultivation in irrigated agriculture in the south of Russia.
Results. It was found that sweet pepper plants grown without seedlings using micro fertilizers and plant growth regulators during the fruiting phase had advantages over the control: in plant height by 6.1; 12.2 and 14.5%, the number of side shoots by 20; 36 and 40%, leaf weight by 5.9; 14.2 and 17.5%, average fetal weight by 3.8; 11.7 and 13.5%. The highest yield of sweet pepper in the experiment (33.6 t/ha), which exceeded the control by 3.2 t/ha (10.5%), and the highest content of vitamin C in the fruits (164 mg%), which is 8 mg% higher than in the control were noted in the variant with the combined use of micro fertilizer and plant growth regulator. Sweet pepper seeds in all experimental variants met the standard requirements (germination rate - 93-96%, germination energy - 93-95%). The highest level of profitability (109.3%) was observed in the variant with the combined use of micro fertilizer and plant growth regulator, exceeding the control by
Relevance. Modern globalization, internationalization, regional competitive differentiation actualize geoeconomic modeling methods for geopolitical planning, management and control. The novelty of the proposed solutions is the proposed concept, based on the translation of the ideas of theoretical mechanics into economics.
Methodology. Considering the annual values of statistical indicators of territorial concentrates as a system of geographically distributed objects (subjects of the Russian Federation), it is possible to determine the geographic barycenter. Knowing the values of the geocoordinates of the barycenter, it is possible to determine the tendencies and trend of the barycenter movement as an indicator of the dynamics of the system under consideration through the analysis of the dynamics of these coordinates. Based on Russtat data on the annual gross harvest of agricultural crops (vegetables), population by constituent entities of the Russian Federation for the period 1991-2021, data on the coordinates of the administrative centers of the constituent entities of the Russian Federation, the values of the geographic coordinates of the barycenter of vegetable growing in Russia were calculated.
Results. For 2021, the geographic coordinates of the barycenter of vegetable growing in Russia are 50.30° N and 49.49° E with an average regional value with an average regional annual vegetable har-vest of 1722.52 million centners/year and fluctuations in the coordinates of the barycenter in the zone of 3-4 geographic degrees or three hundred territorial kilometers with a pronounced south-west trend Analysis of the dynamics of the barycenter for the period 1991-2021. will allow to reveal a slight shift of the vegetable growing barycenter to the south by 3.5 degrees with an increase in the average regional vegetable harvest by 124.11 million centners per year for each degree (or the all-Russian by more than 9,500 million centners per year for each degree to the south), a slight shift of the vegetable growing barycenter to the west by 4.5 degrees with an increase in the average regional vegetable har-vest by 86.5 million centners per year per degree (or the All-Russian by more than 6,660 million cent-ners per year for each degree to the west). For the period 1991-2021, two opposite trends in the devel-opment of vegetable growing are distinguished. In the period from 1991 to 2000, the movement of the coordinate of the vegetable growing barycenter went in the direction of the northeast of Russia, in the period 2000 to 2021 -- on the contrary, to the southwest.
The conclusion is made about the existence of a relationship between the development of vegetable growing and the geopolitics of Russia in the period under review. The obtained method and results can be used in strategic state management of agricultural production in Russia.
Relevance. Garlic is one of the most popular agricultural crops characterized by high nutritional value and unique chemical composition providing significant beneficial effect on human health.
Methods. The review summarizes the most significant publications on garlic biochemical composition, utilization in medicine, human nutrition and agriculture for a period from 1988 to 2024 with the predominant utilization of Scopus and WoS publications.
Results. The diversity of garlic processing products (fresh, dry powder, ‘Green' garlic, Laba garlic, garlic essential oil macerate, ethanolic extract (‘aged garlic extract' AGE) and black garlic) provides the diversity of biochemical composition and the direction of biological effect. The possibility to utilize both garlic bulbs, leaves, stems and peel in medicine and agriculture (for yield and stress resistance improvement) are the most important factors for successful waste free production and wide utilization of garlic.
Relevance. Mushroom farming has the potential to be a very profitable agribusiness venture that addresses several contemporary issues such as resource utilization, circular economy. Increasing mushroom production will increase its availability across the country, which will further help reduce food insecurity and also increase the income of small and marginal producers.
Results. Mushrooms have enormous potential for medicinal and nutraceutical purposes. Over the past 20 years, global mushroom production has grown at a CAGR of 8.26%. This average annual growth rate is the highest for the Asian continent. Mainly six species of mushrooms dominate the global production and market, namely shiitake (26%), oyster mushroom (21%), black ear mushroom (21%), button mushroom (11%), flammulina (7%), straw mushroom (1%) and other types (13%). In the Russian Federation, industrial mushroom growing is actively developing. In recent years, 85 enterprises have been opened. The average annual growth rate of fresh mushroom production was 11.7%. Champignons and oyster mushrooms are mainly grown in Russia. Many obstacles faced by mushroom producers, including lack of technical knowledge, inadequate infrastructure, unorganized market, expensive inputs, etc., still need to be addressed through sufficient research and additional legislative solutions tailored to the requirements of Russian mushroom producers. Growing investments in smart automation to improve the efficiency and productivity of cultivated mushroom production involve advanced technologies such as robotics, artificial intelligence and the Internet of Things (IoT).
Relevance. Using artificial intelligence (AI) systems is of particular importance in the transformation of modern Russian vegetable growing.
Methods. Using the monographic method, the world and Russian practice of developing and using artificial intelligence systems is considered using the example of: CropX, John Deere, IBM Watson, AgEagle Aerial Systems, Blue River Technology, Farmwise, Taranis, Naiad Irrigation, Sustainable Agriculture Technology (SAT), Leader Technology, AgroCalypso, AgroVzglyad group of companies, Russian technologies and systems, Rostec Artificial Intelligence Competence Center, AgroBot, Kaluga Astra, Agrosystems, RosAgro, SAFMAR, AgriCo management company, Sadko agrofirm, AgroEco, AgroInvest and others.
Results, A list of problems with the use of AI in vegetable growing is identified, such as: high initial costs of implementing AI; lack of qualified personnel with the necessary knowledge in the field of IT and agronomy; high probability of technical failures, leading to losses in productivity and increased costs; difficulty of integration with existing automation systems; information security of databases; difficulty of data preparation; legal and ethical risks; lack of necessary infrastructure; unfavorable climatic conditions for the operation of AI; resistance to innovations on the part of personnel. Despite the identified problems, the prospects for using AI in vegetable growing in Russia will allow: optimizing agronomic processes; improving the quality of forecasting and monitoring; increasing the level of automation; improving the quality of data processing; improving resource manageability; increasing the level of adaptation of production to market needs; increasing adaptation to storage conditions and supply chain logistics, increasing the level of information content of technological processes.
Conclusion. Expanding the practice of using AI will increase the efficiency and sustainability of vegetable growing in the strategic perspective.
Relevance. It is necessary to search for methods for obtaining environmentally friendly products and reducing the pesticide load on agrobiocenoses. Biostimulants can influence the increase in the yield of vegetable crops and improve their quality, which is confirmed by numerous studies. Stimulating effects depend on a combination of various biotic and abiotic factors. The aim of the study was to assess the effect of biostimulants on the yield and quality of Brassica Oleracea in the conditions of the Central Non-Chernozem zone on heavy loamy sod-podzolic soils.
Methodology. A field experiment was carried out in the conditions of the Non-Chernozem zone of the Russian Federation on sod-podzolic soil in 2019-2021. In an experiment with two hybrids of Brassica Oleracea: F1 Zarnitsa (mid-early) and F1 Mechta (late-ripening), biostimulants Agrofil and MBK-BIS were studied for soaking seeds, seedlings and foliar treatment. The control variants were without fertilizer (absolute control) and the N90P120K150 background.
Results. The effect of mineral fertilizers on both hybrids was characterized by an increase in the weight of heads of cabbage by 11.7 and 23.5%, respectively. When treating seeds, the weight of heads of cabbage increased by 15-18% compared to the background. Additional treatment with stimulants for soaking seedlings in Agrofil and foliar treatment with the MBK-BIS preparation increased the weight of heads of cabbage in comparison with the background in the F1 Zarnitsa hybrid by 21.4 and 23.3%, F1 Mechta - by 21.4 and 25.4%. The contribution of biostimulants to the yield of the used cabbage hybrids is more pronounced in the late-ripening hybrid, which directly depends on the amount of precipitation during the growing season. The studied biostimulants contribute to an increase in the content of vitamin C, fiber, and the amount of sugars in cabbage heads.
Conclusion. Biostimulants MBK-BIS and Agrofil are effective for increasing the yield and improving the biochemical composition of the studied Brassica oleracea hybrids in the conditions of the Non-Chernozem zone of Russia.
Relevance. A crucial condition for maintaining the vital potential of plants is their ability to with-stand adverse environmental conditions. The aim of the work was to study the effect of the drug "Epin-extra" on strengthening the resistance of Fragaria х ananassa Duch. plants for spring frosts.
Methods. The objects: strawberry garden Queen and Harvest CGL. With the onset of active growth in the spring period non-root treatments of strawberry plants with were carried out with a solution of the Epin-extra growth regulator containing 24-epibrassinolide. During budding phase, the plants were exposed to negative temperatures in a controlled environment chamber (-3oC) "Espec" PSL-2KPH (Japan). The functioning of the antioxidant defense system and the activity of lipid peroxidation processes in membranes lipids (POL) based on the accumulation of malondialdehyde (MDA) were carried out in the leaves of strawberry varieties.
Results. It was shown that the non-root treatment of strawberry varieties with the Epin-extra preparation contributed to a decrease in the processes of peroxidation of cell membranes. The content of malondialdehyde (MDA) was 10,2% lower by the Tsaritsa variety, and the variety the Urozhainaya CGL by 30,1% compared to the control. The amount of the amino acid proline in the Tsaritsa variety was 13,6 % higher compained to the control, and in the Urozhainaya CGL variety by 12,4%. Treatment with Epin-extra contributed to an increase in the amount of sugars in the leaf tissue of both varieties.
AGROCHEMISTRY, SOIL SCIENCE, PLANT PROTECTION AND QUARANTINE
Relevance. Under conditions of prolonged storage of fungi on a nutrient agar medium, their pathogenic properties are gradually lost. Preservation of collection material with unchanged pathogenic properties is necessary for correct research, including the development of biological protection methods, monitoring of pathogen development dynamics, assessment of their virulence and aggressiveness, choice of selection material.
Materials and Methods. The ability to the resumption of full-fledged species characteristics on the culture nutrient agar medium after long-term storage of the biomaterial in the form of a lyophilizate under conditions of low positive +4°C and ultra-low temperatures of -80°C of Alternaria alternata (Fr.) Keissl., Bipolaris sorokiniana Shoemaker and Fusarium solani (Mart.) Sacc. strains stored in the State Collection of Phytopathogenic Microorganisms of the All-Russian Research Institute of Phytopathology (ARRIP) was studied. The viability of fungi was judged by their ability to form spore-forming colonies on a nutrient medium, pathogenicity and phytotoxicity by the reaction of test culture seedlings to the effects of spore suspensions and culture fluid filtrates.
Results. Long-term storage of Alternaria alternata, Bipolaris sorokiniana and Fusarium solani strains in a freeze-dried state, both at low and ultra-low temperatures, did not adversely affect their viability. On the 14th day of culture on a nutrient medium, fungal strains formed sporulating colonies comparable to the control variants. Comparison of pathogenicity signs in fungal strains before the experiment and after storage did not reveal significant differences between the variants. When studying the effect of the culture liquid filtrates of fungal strains on the development of the test culture, a differentiating effect of the storage regime at +4oC was established, while under ultra-low temperatures the results in the variants practically did not differ.
Conclusion. The results of the study were showed that the lyophilization of biomaterial,including imperfect species of fungi for long-term storage at low positive and ultra-low temperatures is relevant and in demand in many scientific and industrial institutions.
Relevance. An increase in the spread of bacteriosis on beetroot in the conditions of the Moscow region carries an epidemiological danger, which actualizes research on the development of a method-ology for evaluating table beet plants for resistance to bacteriosis in the early stages of ontogenesis. The aim of the research. To identify the nature of the relationship between the reaction of sporophyte and microgametophyte to infection with Pseudomonas syringae pv. aptata (Psa) bacterium of beet-root varieties with different resistance of root crops to bacteriosis.
Materials and methods. Objects of research: beetroot plants of the Marusya and Krasny Barhat varietal populations, collection strain Psa 1-21. Using phytopathological methods and methods of gamete breeding, plant resistance was assessed in various variants of infection of sporophyte and gametophyte with suspension and culture filtrate of Psa.
Results. The average volume of the affected area of the Psa root crops of the Marusya variety was 3.5 times greater than that of the Krasny Barhat variety (Vp = 53 mm3), which is characterized by alignment and absence of susceptible forms (Vp >300 mm3). The Marusya variety has a wide intrapopulation polymorphism in the stability of root crops and leaves, where most genotypes showed medium or high susceptibility to the pathogen. Both varieties showed an increase of 10-30% relative to the control of pollen viability at high (CFU 12*108 cl/ml) and low (CFU 2.4*108 cl/ml) concentrations of the pathogen. The positive effect of Psaon the growth of pollen tubes was noted: in the Marusya variety, as the concentration increased, the stimulating effect increased, in the Krasny Barhat variety, it gradually decreased. When adding a 2.5-fold diluted Psa culture filtrate, the susceptible Marusya variety showed an increase in pollen viability (by 3%), and the resistant Krasny Barhat variety showed a 24% decrease relative to the control. According to the growth rate of the pollen tube, the 4:6 dilution was also differentiating, where the average length of the tubes of the susceptible variety exceeded the control by 10%, and in the stable variety it was 18% lower than the control variant.
Conclusion. An inverse relationship was revealed between the resistance of sporophyte to Psa and changes in the functional parameters of the microgametophyte of beetroot varieties Marusya and Krasny Barhat under the influence of a phytopathogen. The results obtained indicate the prospects for the development of a methodology for the selection of bacteriosis-resistant genotypes of beetroot by the reaction of microgametophyte.
IRRIGATION ENGINEERING, WATER MANAGEMENT AND AGROPHYSICS
Relevance. Irrigation of agricultural crops plays a key role not only in increasing the yield of these crops, but also in preserving it, especially under stressful temperature conditions. One of the effective methods of water conservation during irrigation is drip irrigation. Drip irrigation technology allows you to accurately dose water, taking into account the needs of specific crops and maintaining an optimal level of soil moisture. It is important to note that this technology helps save water and reduce the risk of soil overmoistening. In addition to drip irrigation, there is also aerosol irrigation. This irrigation technology allows more efficient use of water resources and provides optimal conditions for the ground air layer for the growth and development of plants under stressful temperature conditions.
Methods. The objectives of the study are to study the technologies and technical means of irrigation of a young cherry orchard to maintain optimal modes through the use of drip irrigation with aerosol irrigation in the Central part of the Non-Chernozem zone of Russia. During the research, two factors were analyzed: studying the impact of various irrigation methods and moistening volumes on the formation of trees of the Gostinets and Zhukovskaya varieties of sweet cherry.
Results. The field experiment is conducted on the territory of «Kolomenskaya Yagoda» LLC in the Moscow Region. The conducted studies have shown that the selected irrigation method using a combined irrigation system on sod-podzolic soils in the central part of the Non-Chernozem zone of Russia for growing a young cherry orchard of the Gostinets and Zhukovskaya cherry varieties meets the criteria of environmental safety and economic efficiency.
Conclusion. From the point of view of profitability, it is most effective to grow a young cherry orchard by maintaining humidity in the root zone of the soil within 80-100% of the lowest moisture capacity and relative humidity of the ground air layer in the range of 55-70%.
Relevance. Lake Ebeyty is the largest salt lake in the Omsk region, located in the south-west of the region, at the junction of the borders of three regions: Poltava, Moskalensky and Isilkulsky, and has huge reserves of medicinal mud. The purpose of the research is to study the characteristics of the chemical composition of Lake Ebeyty water over the years of research (data from 2011, 2019, 2022 and 2023) and soil cover.
Material and Methodology. The paper presents the results of studies of the chemical composition of the water of Lake Ebeyty in the period 2011-2019-2022-2023. In this work, satellite data analysis methods were used to identify relief features and changes in the area of the water surface (archive of satellite images for 10 years) of the study area in the QGIS software product. Water samples were taken during the daytime for chemical analysis using generally accepted methods.
Results. An assessment of the ecological state of the lake was carried out. As a result of the research, it was established: an increase in oxidation from 53.04 mgO2/l in 2011 to 208.0 mgO2/l in 2022, in 2023 the figure decreases to 150.4 mgO2/l, which indicates a deterioration in the ecological situation of the water body. Mineralization increases over the years of research from 128054 to 268691 mg/l (respectively). The soil cover is rep-resented by soils of the saline series - solonchaks and solonetzes.
ISSN 2618-7132 (Online)