BREEDING AND SEED PRODUCTION OF AGRICULTURAL CROPS
Relevance. Modern cultivation facilities require serious costs for their construction and subsequent operation. For the construction of 1 hectare of modern greenhouses with the cultivation of vegetable plants in light culture, about 200-250 million rubles are needed. The payback period of greenhouses is 5-6 years or more, which depends on the output from a unit area of greenhouses in value terms. And the latter, in turn, depends on the crop grown, the timing of its cultivation and receipt of products, as well as yield. Of course, the most important influence on the timing of crop cultivation and cost the resulting harvest is provided by the presence of an artificial lighting system greenhouses and its power. So expensive cultivation facilities should be used as long as possible during the year. Plan most the effective use of greenhouses throughout the year is called cultural turnover. Typically, a culture rotation includes one or more cultures. Time, one culture is called turnover. Throughout the year in each greenhouse plants are grown in one or several revolutions. And to remove or to shorten the breaks between turns on the elimination of old plants, preparation of greenhouses for a new turn and their disinfection use interplanting.
Methods. The experiment was conducted in one of the greenhouses of the Moscow region in 2018 - 2019 in greenhouses equipped with artificial light of 2 hectares.
Results. For various reasons, a number of greenhouse plants refuse Interplanting. Here, from our point of view, there are both objective and subjective reasons. The features of the use of interplanting when growing in light culture will be discussed in this article.
Relevance. The breeding process in identifying new species and flexible capabilities allows the use of genetic sources for the most important economically valuable traits. Market requirements to expand the assortment of cucumber are determined both directly and new approaches to the study of the gene pool and its use. In turn, the domestic market is not always predictable and logical. Thus, the issue of a comprehensive study of the sorting of a gunshot and smooth type of fruit for growing in film unheated greenhouses in order to isolate genetically valuable material for breeding work remains relevant.
Materials and methods. The studies were carried out in 2015-2018 on the basis of a pumpkin crop laboratory of the Research Institute of Vegetable Production (NIISOK), of the Gavrish breeding station in Moscow, Krymsk (Krasnodar Territory), the Gavrish Jordanian Experimental Station. Research material – 34 different types of origin from the NIISOK collection. The work used both traditional methods of assessment and modern methods of molecular marking. The purpose of the work is the selection of breeding valuable source material for creating parthenocarpic lines of a cucumber with a smooth type of fruit, a complex of economically significant features, and resistance to disease.
Results. The results of variety testing of 20 hybrids of foreign selection on the territory of the Krasnodar Territory (Krymsk) and Jordan are presented. A comparative assessment was made of the inter-varietal and ecological variability of productivity and yield of the studied samples of cucumber culture. Based on the results of studying the modern assortment of hybrids in different regions (Krymsk, Jordan), the optimal parameters of the parthenocarpic hybrid model of a cucumber with a smooth type of fruit adapted to specific growing conditions are determined.
VEGETABLE PRODUCTION
Relevance. Vegetables are increasingly recognized as being in demand for food security. Vegetable production reduces poverty and unemployment and is a key component of farm diversification strategies. Vegetables are the most affordable source of vitamins and minerals necessary for good public health. Now is the time to give priority to investments in the production of vegetables, providing enhanced economic opportunities to small farmers and providing healthy nutrition. The purpose of the research is to analyze traditional and new technologies for the production of salad crops (bunch greens, microgreens, baby leaf), to determine the structure of production costs.
Materials and methods. The methods used in economic science were used. The information base of the study was reference materials from specialized publications on the subject under study; materials coming from open and greenhouse vegetable market participants, own research; Internet data (industry portals, sites of manufacturers of open and protected ground products, articles and reviews).
Results. The assortment of lettuce crops, technological parameters for the production of bunch greens, microgreens and seedlings (baby leaf) are presented. It is shown that with proper planning and organization of labor, the production of bunch greenery, microgreen and baby leaf can provide a stable income. The combination of traditional and new technologies for growing green vegetables in open and protected ground conditions can provide the market with high-quality products. Continuous improvement of new technologies, reducing the cost of consumables, economical use of energy, water and increasing labor productivity contribute to lower costs and product prices.
Relevance. A priority in the selection of watermelon is the creation of new competitive varieties that combine resistance to adverse stressors, high yield and excellent fruit quality. The purpose of this study is to show the influence of weather conditions on the growing season, yield and quality of watermelon fruits and the influence of growing conditions on the growth and development of watermelon plants.
Materials and methods. The study used 5 varieties of watermelon included in the State Register and 2 new varieties, ready for transfer to the State Variety Test. During the research, all observations and counts were carried out in accordance with the State Variety Testing Methodology and the Field Experience Methodology.
Results. In the process of research, it was found that the length of the growing season largely depends on the amount of precipitation and the sum of active temperatures, as well as on their distribution in interphase periods. It was noted that in the year with the highest rainfall, the growing season was the longest. Productivity for the analyzed period varies from 14.2 t / ha to 20.7 t / ha in the early group, from 19.4 t / ha to 24.9 t / ha in the average. The highest yields were recorded in 2017 with the least rainfall. Growing conditions had a significant impact on the quality of watermelon fruit: the solids content ranged from 9.4% to 12.8%, total sugar - from 7.70% to 11.35%.
Relevance. The annual increase in the area under the eggplant culture in small farms and the individual sector is explained by high nutritional, dietary, taste, and also the multi-purpose use of fruits. For optimal growth and development, eggplant needs a high amount of active temperature, which greatly limits the cultivation of crops in many regions of Russia. However, the egg-plant growing area is expanded through the use of industrial and film unheated greenhouses. The development of technology elements for growing eggplant in film greenhouses is relevant, as it contributes to an increase in the area of farms.
Materials and methods. The aim of the research was to substantiate the elements of the technology for growing eggplant in film unheated soil greenhouses. The studies were carried out in 2018-2019 at the base of the Educational and Scientific Production Center “Vegetable Experimental Station named after IN AND. Edelstein» RGAU-Moscow Agricultural Academy named after K.A. Timiryazev in the summer-autumn turnover in a film unheated soil greenhouse. The objects of research were varieties and F1 eggplant hybrids: F1 Borovichok, F1 Bagira, F1 Tender, F1 Patio Trio, as a control they used a hybrid F1 Pelican; eggplant varieties: Mushroom pleasure, Black handsome, Don Quixote, they used the Frant variety as a control.
Results. In general, based on the results of studying the influence of the formation of two and three stalked eggplant varieties and hybrids on the total yield in the conditions of summer-autumn turnover in film soil greenhouses, we recommend that all studied hybrids and varieties be grown in three shoots.
MEADOW AND MEDICINAL ESSENTIAL OIL CROPS
Relevance. Common thyme or garden thyme Thymus vulgaris L. (Lamiaceae L.) is the medicinal and aromatic plant containing essential oil which is the source of thymol and other phenol derivatives. This species is characterized by significant morphological and chemical polymorphism. Thymus vulgaris L. has numerous varieties and subspecies which are complicates for the identification of raw materials and makes its quality unstable. The study of intraspecific variability, not only by phenotype but also by biochemical parameters, is an urgent task in the search for varieties and samples of Thýmus vulgáris L. that are promising for the medical industry.
Materials and methods. The research material was obtained from botanical institutions and firms in Russia, the Czech Republic and Germany and introduced on the experimental field of Vegetable Growing Department of The Russian State Agricultural University – Moscow Timiryazev Agricultural Academy. Studies were conducted from 2014 to 2019. Sowing the seeds was carried out in the 3rd decade of March in cassettes in a winter greenhouse. Seedlings planted in the field at the end of MayField experiments were laid at the «Vegetable Experimental Station named V.I. Edelstein » in accordance with generally accepted methods of field experiments. A comprehensive comparative assessment of the studied samples was carried out according to a number of criteria, in accordance with the recommendations of the State Register of Selection Achievements and the matrix of the morphological description of plants of the Lamiaceae family developed at the Leibniz-Institut for Pflanzengenetik und Kulturpflanzenforschung (IPK, Germany). Harvesting of raw materials and crop yield was carried out in the phase of mass flowering. Laboratory studies were carried out in the laboratories of the Vegetable Growing Department, Department of Botany, Selection and Seed Production of Garden Plants, Russian State Autonomous University – Moscow Agricultural Academy named after K.A. Timiryazev. The quantitative determination of essential oil was carried out according to the GF RF XIV. Statistical analysis was performed using Microsoft Excel.
Results. The possibility of growing samples of Thymus vulgaris L. of different geographical origin under the conditions of introduction in the Moscow region is shown. A comparative study of the main morphological characteristics of plant samples of Thymus vulgaris L. revealed significant variability in the form of inflorescence, leaf index, the presence or absence of pubescence, anthocyanin staining, torsion of the leaf, color of corollas and leaves, plant height, yield, quantitative content of essential oil and flavonoids. At the same time, the height of the plants was relatively stable every year and between varieties. According to a set of indicators, the most productive and stable samples was “Di Roma”, which is characterized by a high yield of aerial mass (104 g / plant), a consistently high content of essential oil (1.04-1.8%) with a predominant thymol component (39-80%), high flavonoid content (1,47-2,26%). Varieties "Medoc" and "Deutsche Winter", which at a lower yield are characterized by a high content of essential oil and flavonoids.
Relevance. The degree of variability of the linear parameters of the morphological elements of the seeds of the anise (variety Vityaz) and cumin (variety Peresvet).
Materials and methods. 1) Plants: Monarda fistulosa L. (the breeding sample №5 U.P.), Monarda citriodora Cerv. ex Lag. (Simka variety), Melissa officinalis L. (Zhemchuzhina variety). 2) secondary metabolites: flavonoid glycoside linarozid and steroid glycoside moldstim.
Methods. The studies were carried out in VNIIO – a branch of the FSBSI Federal Scientific Vegetable Center. The measurement of the length of the seed and endosperm was carried out using a caliper (GOST 166-89). The embryo length was determined using a Levenhuk 670T microscope and a DCM 300 MD video eyepiece at Ч 40 magnification using Scope Photo (Image Software V. 3.1.386). The lengths of each seed, endosperm (longitudinal section) and embryo (after isolation) were sequentially analyzed. The indices IE/C, IZ/E, IZ/C were calculated as the corresponding ratios of the length of the seed, endosperm and embryo.
Results. The maximum length of the seed (4.76±0.10 mm) and of the endosperm (4.08 ± 0.08 mm) and the length of the embryo (1.40±0.02 mm) were noted in cumin seeds. The average values of the coefficient of variation (V), on the contrary, were minimal for cumin seeds, varying from 7.8% for the length of the embryo to 11.5% for the length of the seed. The average values of the coefficient of variation (V) for anise varied from 9.3% for the length of the embryo to 14.7% for the length of the seed. The maximum level of variability in both cultures was noted for seed size. Correlation analysis showed that a close relationship was observed in anise (r=0.912) and cumin seeds (r=0.876) only between the length of the seed and the length of the endosperm. The value of the correlation coefficient (r) between the linear sizes of the seed and the embryo was 0.195 for anise and 0.229 for cumin seeds, and between the length of the endosperm and the length of the embryo, 0.237 and 0.214, respectively. The correlation coefficient (r) between the index IZ/E and the linear sizes of the seed, endosperm and embryo had low negative values from -0.221 to - 0.345.
RECOVERY, RECULTIVATION AND PROTECTION OF LANDS
Relevance. The Volgograd region is one of the major producing regions of table carrots with a difficult climate, therefore, regional adaptation of technology parameters and optimization of reclamation regimes under drip irrigation is necessary. According to most researchers, it is important to take into account the weak development of the root system of carrots in the initial periods of plant growth and development, so the differentiation of the moistened soil layer will allow more rational and productive use of soil moisture.
Materials and methods. The working hypothesis of the studies was the assumption that it is possible to intensify the production process of carrots when managing water and mineral nutrition by drip irrigation against the background of high-quality soil preparation with the formation of ridges and the use of modern sowing units. In accordance with the hypothesis put forward, the research program is based on the assessment of threshold values of the conditions for the appointment of vegetation irrigation with drip irrigation and doses of mineral fertilizers.
Results. The determination coefficient of the estimated dependences of the carrot water consumption coefficient on the duration of maintaining the starting irrigation regime and mineral nutrition conditions is 0.87-0.94, which characterizes a strong relationship between the indicators included in the equation. it was beneficial for Cordoba F1 hydride to maintain the starting regime of carrot irrigation, oriented to moisten the calculated 0.2-soil layer, to the stage of formation of the 2nd leaf, as well as to apply mineral fertilizers with a dose of N240P90K315 of the planned yield level, 120 t/ha was not received. The productivity of other hybrids studied in the experiment did not exceed 100 t/ha; therefore, it is advisable to introduce mineral fertilizers with a dose of N190P75K245, calculated specifically for this level of planned yield.
AGROCHEMISTRY
Relevance and methods. Methodological approaches to assessing the residual amounts of copper-containing pesticides in plant products against the background of natural copper content are presented. The conditions for obtaining reliable results when conducting a hygienic assessment of the content of residual amounts of the active substance are determined. The developed scheme for determining the residual amounts of copper-containing pesticides includes the homogenization of the analyzed material by shredding with dry ice and microwave mineralization of samples with subsequent flameless atomic absorption determination of copper.
Results. Data on the levels of natural copper content in samples of potatoes, onions, cucumbers, tomatoes and grapes of various varieties and growing areas are presented. The possibility of applying the developed approaches to study the dynamics of accumulation of active substances during the growing season is shown.
Relevance. Biofortification of agricultural crops with selenium is considered to be the most promising method for the human selenium status optimization.
Methods. Effect of foliar biofortification of cauliflower, Polyarnaya Zvezda cv, with sodium selenate of different concentrations on yield, selenium content and biochemical characteristics of plants were investigated.
Results. Enrichment of plants with selenium increased yield by 1.23-1.31 times, sugar content – 1.6 times, ascorbic acid concentration – 1.52-2 times. On the contrary, the treatment did not affect pholyphenol content and antioxidant activity of ethanolic extracts of plants. Selenium accumulation levels decreased according to inflorescences > leaves > roots. Sodium selenate solution at 75 mg/L concentration increased mass of cauliflower leaves 1.9 times and roots – 1.5 times. Consumption of 100 g of cauliflower fortified with 50 mg/L sodium selenate solution provided 100% of the daily adequate selenium consumption level. Utilization of higher sodium selenate concentrations ensured 127% and 418% of the daily adequate consumption level in case of 75 mg/L and 100 mg/L concentrations respectively. Taking into account insignificant differences between sugar content and antioxidants in cauliflower inflorescences fortified with different doses of selenium the most suitable concentration to be used was 50 mg/L.
Relevance. This article presents the results of a study of the process of sucrose inversion in vegetable and fruit monocomponent purees with and without sucrose under the influence of ultrasonic action on them.
Methods. For the study, experimental samples of carrot, pumpkin and Apple puree of two types were made – a monocomponent natural puree (without sugar) and puree with the addition of 5% sucrose (by weight). The main part of the study was to study the depth of sucrose inversion in experimental samples, depending on the specified parameters of ultrasound exposure at a frequency of 21.6 kHz for 30 minutes on a laboratory cavitation unit in a flow-through cyclic mode.
Results. It was found that in all experimental samples, the amount of total reducing sugars increased significantly compared to the values obtained in control samples that were not subjected to ultrasonic treatment. So, in natural apple puree (without adding sucrose) after ultrasound exposure, an increase in total reducing sugars was detected by 9.3%, and in experimental samples of apple puree with added sucrose – by 10.9%, in relation to control samples, which is 1.2 times higher than in natural puree. In samples of carrot puree (without adding sucrose), the increase in total reducing sugars was 6.7%, and in puree with added sucrose, the increase was 12.2%, which is 1.8 times higher than the inversion in puree without sugar. In samples of pumpkin puree without sugar, the increase in reducing sugars was 4.2%, and in samples with added sucrose – 9.9%, i.e. 2.4 times higher than in samples without sugar.
These data allow us to conclude that ultrasound exposure can intensify the inversion of sucrose with an increase in the number of reducing sugars, and this process takes place more deeply in products containing added sucrose.
PLANT PROTECTION
Relevance. The use of chemical fungicides in protecting plants from pathogens is the most common way, despite the proven danger of pesticides. The use of biofungicides based on essential oils of medicinal plants, which also have phytoncidal properties, can be a suitable alternative to chemical preparations. In the years favorable for the development of alternariosis or cladosporiosis, spots appear on the plants, leading to necrosis and significant loss of seed productivity. Therefore, the search for new effective and safe means of combating fungal phytopathogens becomes relevant.
Methods. Alternaria alternata (2018-1.1) and Cladosporium cladosporioides (2019-11.4) strains were isolated from leaves with signs of alternariosis and cladosporiosis, on which the essential oils Abies alba, Artemisia vulgaris, Caryophyllus aromaticus, Coriandrum sativum, Lavandula officinalis, Ocimum basilicum, Pimpinella anisum, Rosmarinus officinalis, Salvia sclarea, Thymus vulgaris. The antifungal activity of essential oils was evaluated by comparing the diameter of the experimental (with the addition of essential oil to the Petri dish) and control colonies using the author’s scale.
Results. All studied essential oils had antifungal properties, but species-specificity was observed. Essential oils of basil, coriander and thyme showed a fungicidal effect on C. cladosporioides and A. alternata. Lavender oil proved to be a fungicide for A. alternata and a strong fungistatic for C. cladosporioides. To create biological products for plant protection against alternariosis, sage essential oil (moderate fungistatic effect) may also be of interest, and cladosporiosis - anise and rosemary oils.
Relevance. Late blight (Phytophthora infestans Mont. de Bary) is one of the most harmful, rapidly spreading potato fungal diseases, which dramatically reduces its yield and affects tubers during storage. The quantitative manifestation of partial resistance within the same potato variety depends on meteorological conditions and the infectious load. Therefore, its objective assessment is obtained when testing varieties in regions that are stably favorable for the development of pathogens (Sakhalin Island and central Mexico), or in standard laboratory conditions using climatic chambers and molecular genetic methods. The aim of this work was a laboratory assessment of the potato varieties and hybrids resistance to late blight.
Methods. Data on laboratory assessment of the potato varieties and hybrids resistance to late blight are presented. The experiment was carried out using generally accepted methods for the study and assessment of the potato varieties resistance to phytopathogens with minor modifications.
Results. It was established that the varieties Smak, Yantar, Dachny and Kazachok are medium susceptible to late blight, as shown in the Catalog of varieties. In the variety specimen Pri-12-35-4, with a low degree of necrosis and sporulation development, slices showed damage to 50% of the tubers surface with active sporulation and maturation of double-flagellated zoospores. Hybrids Pri-11-12-5, Pri-12-18-7 showed a high score for the development of necrosis and sporulation with a slight excess to these indicators for tubers (1.17-1.05 times). Among hybrids and varieties of potatoes, a sample of Pri-11-31-24 stood out, with an average score for sporulation and slice necrosis of 1.44 and 1,42, respectively, for tuber damage - 1,72.
Relevance. It was to study the variability of the degree of defeat of promising varieties of table carrots by diseases of various etiologies during storage for several years, to determine the level of their polymorphism based on resistance to various types of pathogens and the effectiveness of selection based on an individual immunological assessment of uterine root crops.
Materials and methods. The experimental part of the work was performed in 2017-2020 at the FSBSI "Federal Scientific Vegetable Center" with carrot varietal populations of Margosha and Minor. The identification of the species composition of pathogens, the degree of damage, and the level of resistance of variety specimens were carried out in accordance with appropriate methods and determinants. For the immunological assessment of the resistance of carrot cultivars in vitro, the most virulent isolates of different types of fungi were used from the collection of the Laboratory of Immunity and Plant Protection of the Federal Center for Scientific Research.
Results. It was established that in the populations of Margosha and Minor under natural storage conditions, the ratio and degree of spread of economically significant diseases differ significantly in different years. Based on an in vitro immunological assessment, a high polymorphism of the individual resistance of genotypes within both populations to the majority of micromycetes was revealed and the most resistant forms to the most dangerous types of pathogens were identified. Immunological analysis of the resulting offspring confirmed the high efficiency of group selection based on a comprehensive assessment of the stability of individual uterine root crops in vivo and in vitro.
AGRICULTURE
Relevance. The aim was to study the reaction of new potato varieties early ripening group Zabava and middle-ripening group Smolyanochka on the timing of planting and doses of mineral fertilizers for obtaining maximum yield of seed potatoes. As mineral fertilizers used azophoska, superphosphate and potassium chloride.
Results. It was found that the variety Zabava compared to the variety Smolyanochka, had a higher stem-forming ability and exceeded the parameters of the stem in all variants of the experiment. This is due to the biological characteristics of the studied varieties, since the Zabava variety, unlike the Smolyanochka variety, had more multiple eyes on the surface of the tuber. Against the background of applying mineral fertilizers N60P72K90, the density of the stem was higher, compared with the background of applying N32P32K32 in both the Zabava variety and the Smolyanochka variety. It should also be noted that there was a tendency to reduce the stem at a later date of planting in all variants of the experiment. The percentage of viral diseases affected increased with later planting dates and was highest with the planting dates of 14 and 20 may. The highest yield of tubers of the seed fraction was obtained in the variant with a planting date of may 14 against the background of balanced doses of mineral fertilizers (N60P72K90) and amounted to 13.3 t/ha in the Zabava variety, 13.0 t/ha in the Smolyanochka variety. It should be noted that different backgrounds of mineral fertilizers did not have a significant impact on the percentage of potato tubers affected by diseases. At the same time, the incidence of tubers with diseases did not exceed the regulatory requirements of GOST R 53136 – 2008 "Seed potatoes. Technical conditions».
Relevance. In the difficult natural and climatic conditions of the Far Eastern region of the Russian Federation, there is a need for a comprehensive study of the source material to create modern competitive varieties with ontogenetic adaptation that can adapt to external conditions. The relevance of research is to mobilize the potato gene pool and study promising source material for potato breeding.
Materials and methods. The goal of research is to identify sources of economically useful traits based on a comprehensive study of the source material to create high-yielding varieties of potatoes resistant to negative bio- and abiotic environmental factors. The object of research is domestic and foreign varieties of the collection nursery of potato of the All-Russian Scientific Research Institute of Soybean. The studies were carried out in the natural and climatic conditions of the Amur region on meadow chernozem soil of the experimental field of FSBSI ARSRI of Soybean in 2015-2019 years in accordance with the methods generally accepted for potato culture.
Results. Varieties Fermer, Yubilyar, Zhukovskiy ranniy, Krinitsa, Rodrigo, Natasha, Delphin and Zolskiy showed stable productivity by years of research and the variation coefficient of the trait was 10.8...18.1%. Stable high tuberous productivity of plantings over the years was identified in varieties Zolskiy, Rodrigo and Natasha – 27.0-28.8 t/ha, its variation coefficient was from 15.4 to 18.1%. Varieties Delphin, Impala and Vulkan were characterized by high (over 98%) marketability of tubers. The use of the specified collection samples as parent forms when crossing in various hybrid combinations will make it possible to obtain highly productive offspring for further use in potato breeding.
ISSN 2618-7132 (Online)