BREEDING AND SEED PRODUCTION OF AGRICULTURAL CROPS
On April 18th, a doctor of agricultural sciences, professor, a member of Russian Academy of Sciences, academician, Honored Research Worker in Science and Technology, State Laureate of the Russian Federation Government Prize in Science and Technology, a director of Federal State Budgetary Research Institution ‘All-Russian Research Institute of Vegetable Breeding and Seed Production’, Victor Fedorovich Pivovarov celebrates his 75th birth anniversary.
The current state of seed market of vegetable and gourds in Russian Federation in the frame of national food safety program is given in the article. Russia as a country with well-developed seed production in the last century has now ceded its position, and according to last experts’ association estimations the 80% of required volume of seeds of vegetables and gourds, amounting from 8 to 12 thousand per year is imported. Not less than 15 thousands of hectares are needed to be necessary for demand of Russian seed production sector, however, presently only 2 thousands of hectares is a total certified land used for seed production. Moreover, the seed production sector of some countries that export seeds rises becoming a profitable branch of agriculture, and providing local employment. The lack of competitiveness in Russia causes that many foreign companies increase annually purchasing prices for seeds and their production services. Therefore, now the total volume of seeds imported in Russian Federation has nearly been twice less for the last years than in 2012, but in currency earnings in exporting countries the insignificant changes can be seen, where deviation is only 19-25% from average annual value, that means 1675 thousand dollars per year. Besides, for the Russian budget in the ruble currency the total cost of imported seeds has become 2.5 times more expensive since 2012, but the increase of combined cost of marketable vegetables has been over 2.7 billion of rubles. The main idea that the seed production is a process requiring the participation of breeders, seed producers and seed companies is main factor to succeed in recovering seed production sector. Exception of any participant or ignoring his interests may destroy all process. For instance, there is a chronic problem of plagiarism and royalty nonpayment causes the break between the business and breeder, affecting on the final result. It is evident that the development of National Associations for Professional Unions of breeders, seed growers and businessmen with active participation of the governments in building regulatory and legal base, rules and mechanisms of relationships for all partners in seed market enables to develop the effective and stable national system of seed production.
The crops of Brassica rapa L. species are varieties such as pak-choi, tatsoi, wutacai, tsoisum, mizuna, mibuna, brokkoletto and Japanese leafy turnips Komatsuna, Kurona, Hiroshimana, Shirona, Mana, which are all characterized by early-ripening, high productivity, presence of valuable biochemical compounds and relatively simple growing requirements. The nappa cabbage is widespread and cultivated everywhere in the world as open field and greenhouse crop. Other varieties are grown locally, namely brokkoletto is grown in Italy, whereas other mentioned varieties are mostly cultivated in the Southeast Asian countries, where population uses them for different processing technologies. There is the nappa cabbage that is mostly cultivated, particularly for industrial production in Russia. The Chinese cabbage (pak choi), Japanese cabbage, Japanese mustard spinach or Komatsuna are not very widespread and practically unknown crop. However, vegetable growers are interested in using new cole crops, and gardeners know about values of related varieties of nappa cabbage in the group of Asian cole crops. The analysis of incoming genetic accessions of Brassica rapa L. crops that have been included into the VIR plant collection is given. All botanical subspecies and varieties of leafy varieties have been taken for the study. The detailed description of new for Russia varieties, such as purple, brokkoletto, rosette pakchoi as well as well as types of cultivars that haven’t still included into State Register of Breeding Achievements of Russian Federation are given. According to research results obtained at Pushkin VIR laboratories (Saint-Petersburg) the initial breeding accessions have been selected to be sources of such characteristics as productivity, early-ripening, disease resistance and valuable biochemical compounds.
The main problem of agroindustrial sector of national economy is an absence of sufficient production of plant proteins that are used for food production as well as a forage production. New cultivars of legumes, namely in soybean have to be developed to resolve this task. Soybean seeds contain much protein up to 50%, fat up to 28 %, along with vitamins, phospholipids, isoflavones and ferments. Soybean is a short-day crop. The main reason that limits the spread of soybean is the absence of new early-ripening cultivars. Folk cultivars and varieties native to China produce the grain yield at the sum of temperatures over 3500oC. At the beginning of the last century, there were bred the varieties that produced the grains at the sum of temperatures below 3000oC. Only after that the soybean as a crop is started to be introduced in different countries. The study on association among quantitative traits is needed to select necessary accessions for breeding program. In this research estimation of correlationship based on data of structure analysis was carried out within two-year period. The correlationship of economically valuable traits, such as 1000 seed grain weight, number of grains in bean (pod) and other traits such as plant height, stem thickness, the height of pod attachment, a number of branches, number of pods on the plant, number of productive nodes, average number of pods at node, average number of grains (beans) per plant in field experiment in 2015-2016, Central Zone of Non-chernozem Zone of Russia. It was noticed the difference in trait correlationship depended on a year, and it needs to be taken into account for realization of breeding program in soybean.
Recently, the particularities in carrot trait variation, namely root color and shape; carotene content; duration of vegetative period; resistance to diseases and pests have been established. But such traits as yield capacity, root marketability, root quality, long-storability as well as their genetic determination haven’t been well studied. Consequently, thorough study of genetically diverse accessions and the search for phenotypically well-identified traits are very actual tasks. The ecological and geographical study of carrot enables to reveal polymorphisms among cultivars, in order to use it in the breeding program. The study of collection accessions of local and foreign origin in contrasted conditions varying in temperature and water regimes revealed the significant variation of main biological and economically valuable traits, including root yield and quality. The root yield was substantially higher in Pushkin (Leningrad oblast) than in Vologda oblast. Comparatively stable yield and quality were shown in local cultivars: ‘Nanatskay Krasnaya’, ‘Princess’ and ‘Gribovskaya’. Very important requirement for highly marketable products is the cultivar resistance to disease and pests. There are no completely resistant genotypes to carrot psylla among accessions studied. Carrot accessions bearing resistance to the pest and also showing high productivity and quality were ‘Losinoostrovskaya 13’ (Russia), ‘Gavrilovskaya’ (Ukraine), ‘Feonia’ (Denmark), ‘New Model’ (UK), ‘Betina’ (the Netherlands) that can be used for breeding program. ‘Krasnaya Dlinnaya’ (Russia) and ‘Chantenay а Coeur Rouge’ (France) were comparatively resistant to powdery mildew.
Tree-shaped plants of tomato have many useful traits, which gain them advantages over common tomato forms. In world plant collection there are over 200 accessions, cultivars and hybrids of tree-shaped tomato. The aim of the study was to develop new initial tomato accessions for breeding program for tree-shaped cultivars and hybrids that are distinguished by a shape, fruit weight and color, improved by interspecific hybridization chemical composition and resistance to the major diseases. The tree-shaped breeding lines: ‘196/12’, ‘374/08’, ‘909/14’, ‘911/14’ and other were observed with the use of interspecific hybridization and selection in the population F2-F4. The selected lines passed the trial in artificially infected condition with Alternaria, viral diseases (MToV, TSWV) and phytoplasmas. Among breeding lines assessed the ‘911/14’, ‘374/08’ and‘40/11’ were less affected by complex of pathogens. The lines ‘196/12’ and ‘909/14’ passed an assessment in the nursery for variety trial in naturally and provocatively infected conditions with major pathogens. Both breeding lines had an advantage over standard accession ‘Laguna’ and ‘Maraphon’ for total yield capacity and standard fruit harvest; it was more by 9.6% and 52.2% and 9.5% and 53.4% respectively. Fruits of lines 196/12 and 909/14 had good taste quality with high content of dry matter (5.8% and 6.8%), sugars (3.3% and 3.1%), vitamin C (22.2 and 24.8 mg/100 grams). The selected tree-shaped accessions have been used to develop heterotic hybrids and to be sources of economically valuable traits.
Results of estimation of general and specific combining ability of male and female parental tomato forms were given for complex of traits. The estimation was carried out according to incomplete diallel crosses 15x15. Such varities as ‘Trapeza’, Rosovaya kapelka’, ‘Seniorita’, ‘Ocharovanie’, ‘Tigris’ ‘Vishnya Zheltaya’ (Gavrish), ‘Denezhnoye Derevo’ (national breeding), and lines: ‘46/06’, ‘49/09’, ‘295/09’, ‘336/11’, ‘354/11’, ‘357/11’, ‘388/09’ (nor), ‘498’ (selection of TARI) were used as intial breeding accessions, differing in bush type (determinate and indeterminate), duration of vegetative phase (ultraearly, early, medium early, middle-ripening), fruit shape (rounded, oval), fruit color (red, pink, black, orange, tiger and with the gene nor), fruit weight (10 g. and more), brush structure (dense, friable). As a results, ‘Trapeza’, ‘Vishnya Zheltaya’, ‘Ocharovaniye’, ‘Seniorita’, and lines: ‘295/10’, ‘49/09’, ‘498’, ‘357/11’, ‘354/11’, ‘388/09’ (nor) were selected and recommended to be used in breeding program for development of heterotic hybrids with high fruit setting and generative bush type. The promising hybrids F1 with high constants of specific combining ability for a complex of economically valuable traits have been observed. Because of different lines and accessions were used in crossings, these hybrids varied in internode length of cluster type, classical or shorten; rounded or oval fruit shape; fruit color, red (F1 combinations ‘354/11’ х ‘Seniorita’, ‘Trapeza’ х ‘L.49/09’, L. ‘49/09’ х L.354/11), pink (F1 combination ‘Rosovaya Kapelka’ х ‘L.354/11’), yellow (F1 combination ‘Ocharovaniye’ х ‘Vishiya Zheltaya’), deep brown (F1 combination ‘L.357/11’ х ‘L.354/11’).
Presently the X-ray separation is used not only for research program, but it is also elaborated and applied for different sectors of economy. The seeds as biological objects that possess the complicated microstructure are very difficult to be exanimated by x-ray technology. The application of x-rays and further elaboration of optical separators, principle of action, basic specifications, way of their use and their efficiency was shown in the article. The x-ray separator may distinguish all hidden seed defects as it was described by a programmer, where owing to the use of the optical separating block in visual range it is possible to add some more details as a shape, brightness and a color of object surface being exanimated. The elaboration of such separation equipment is scientifically hard work requiring time and expenses. Last year researchers of ‘LETI’ developed the working model of industrial x-ray separator for examination of grains and nuts in different crops. This model was made on the basis of photoseparator F-5 manufactured at OAO ‘Voronezhselmash’. The instrument state and its mechanism operation are highlighted on monitor. In the regime of processing (separation and examination) of each controlled batch, the passport is produced with following information on identification code, time of material receiving, time of test passed, number of grains or seeds tested. The code of receiver of material is given to each of established characteristics when working the regime of separation, determination of number of objects with characteristics tested and number of unidentified objects. The application of x-ray separators constructed on the basis of photoseparator F-5 enables to carry out the complex estimation on seed quality and separation in only instrument with the development of electronic protocol with many characteristics.
VEGETABLE PRODUCTION
Brussels sprout (Brassica oleracea convar oleracea L. var. gemmifera DC) is a highly valuable cole vegetable crop, which is grown widespread in Europe and cultivated in gardens in Russia. The breeding program for this crop has been carried out at VNIISSOK. The biological features, useful properties of Brussels sprout along with agrotechnical instructions for cultivation were given in the article. The modern trends in breeding program for the crop were shown. The description of cultivar ‘Hercules’ and heterotic hybrid F1 ‘Sozvezdie’ bred at VNIISSOK was given.
Napa cabbage is well spread in Japan, China, Korea, but it is not so well known in Far-Eastern regions of Russia. Since 2012 the collection of hybrid accessions has been evaluated at agrobiological station of Blagoveschensk State Pedagogical University located in western part of Blagoveschensk. Twenty local and foreign hybrids have been studied. As a result, seven highly-yielding accessions of different terms of maturity have been selected and included into State Register of Breeding Achievements of Russian Federation. The complex assessment of most promising hybrids for uniform heads and excellent consumer’s characteristics has been performed under the condition of south zone of Amur oblast.
In 2014-2016 the assessment of kidney bean cultivars bred at Omsk State Agrarian University on yield capacity, quality, and biochemical chemical compounds in green beans, such as proteins, zinc, iodine, iron and sucrose was carried out in the south forest-steppe of Western Siberia. The technological parameters of green beans: the shape of cross-section area; pulpiness, fibrous or non-fibrous, and bean thickness were estimated. Threeyear study showed that all tested cultivars bred at Omsk State Agrarian University surpassed control cultivar in yield capacity, chemical composition and technological parameters of green beans. The cultivar ‘Pamiyaty Rizhovoy’ gave a highest yield: 563.4 g/m2 in 2014; 622.8 g/m2 in 2015; and 620.4 g/m2 in 2016. It is worth noticing that this cultivar is also distinguished by contents of micro and macroelements: 21.20-28.68 mg/kg of zinc; 0.012-0.018 mg/kg of iodine; and 1.8-3.2 mg/kg of iron. In the course of the study, it was noticed the dependence of yield capacity on hydrothermal coefficient (HC). In 2014-2015 the yield capacity was a lowest when the moisturization was insufficient at 0.7 HC. With increase of HC the yield was higher. With sufficient moisturization at 1.0 HC the highest yield of green beans was observed in 2016. The estimation of kidney bean cultivars bred at Omsk State Agrarian University showed that all beans were distinguished by high quality parameters in the phase of technical maturity, such as pulpiness, nonfibrous, thickness 0.5 to 1.0 cm, and green and yellow colors. The highest protein content comparing to the control cultivar ‘Zolushka’ was detected in ‘Marusya’ 23.60% in 2014, 20.94% in 2015; and ‘Zoloto Sibiry’ 19.79% in 2016. The observed results confirmed that contents change of proteins, sucrose, and even micro and macroelements depended on the climatic conditions. In the hot and dry summer the protein content, depending on cultivar increased 18.31% to 23.60%, in 2014, and 17.81 to 20.94% in 2015. The same occurred with zinc 19.63 to 28.68 mg/kg in 2014, and 20.14 to 27.54 to 2015; and iron 2.0 to 3.2 mg/kg in 2014 and 1.6-1.9 mg/kg in 2015.
The squash is one of the most ancient plants, bearing numerous qualities. The squash gave high yield and is unpretentious plant, the fruit of which is long-stored and can be used as boiled, stewed, baked, fried product, and be added to bread, mush and pudding, and be prepared as puree, jam, candied fruit and other meals. Owing to useful characteristics the squash is a valuable source or raw material for food canning industry, for health-care and functional foodstuff production. Its best cultivar accessions contain to 30% of dry matter, to 12% of sugars and to 36% of carotene. The species of squash includes 4 subspecies and 20 varieties. One accession that came from Spain in 1937 served as an initial breeding accession for many other table cultivars for long time. For 80 years of national breeding program the many cultivars for table use have been released. There are the classic cultivars, such as ‘Mramornaya’, ‘Stolovaya Zimnaya’, Gribovskaya Zimnaya’, ‘Donskaya Sladkaya’, ‘Michurinskaya’ that are selected or developed from first Spanish accession. This richness and originality of cultivar squash accessions should be carefully preserved. There are the new generation cultivars, such as ‘Kroshka’, ‘Malishka’, ‘Rossiyanka’, ‘Konfetka’, ‘Moskvichka’, ‘Vnuchka’ and many others, which are short-tendrils and suitable for modern mechanized cultivation technology. Moreover the development of new cultivars, bearing resistance to biotic and abiotic stress is the main goal of nearest breeding program.
The potato is grown in all natural environment and climatic zones, along from high-mountain slope lands, situated up to 2500 meters above sea level to Pricaspian plains, situated under sea level. The total area of planted potato in the Republic is 22 thousand hectares, where potato producing takes more than half of mountain region. The mountain area occupies 2.04 million hectares, which is 38.3% of total area of Dagestan with highest peaks over 1000 meters above the sea. There are some methods of potato growing that are nearly effective in all climatic conditions, and improve yield and tuber quality. The proposed resource-saving technology enables to reduce expenses by 50 % for ploughing, soil preparation after ploughing, pre-planting soil preparation, preserving soil fertility and decreasing erosion processes. The results based on experimental data presented were to show the influence of resource-saving technology on growth, development and yield augmentation when growing on slope lands of mountain province of Dagestan. Advantages of this resource-saving technology and its economic efficiency were shown as well.
Technology of intense light-culture has been developed to grow different crops under controlled environmental conditions with the use of specialized vegetation apparatus (VSU). In the specialized vegetation apparatus, designed to grow plants of cucumber, tomato and leafy crops, the formation of optimal light condition is constructively made with application of separate powerful lamps that have the same or different spectral quality irradiance, providing the high illuminance, 60-90 W/m2 PAR on cultivated plants. The vertical vegetation apparatus were used to develop technology of intense light-culture in cucumber. Three modifications of vertical vegetation apparatus differing in soil blocks and the way of nutrient supply to root habitation area were elaborated to grow the plants of cucumber. The development of cucumber plants in three apparatus modifications was carried out by one stem, where the growth was limited by the sizes of apparatus to 1.8 meter. The result of development of small-volume and non-soil technology of intense lightculture in cucumber was presented in the article. This technology provides high productivity of plant being grown and reduces the ecological problems related to waste treatment. The application of technology of intense light-culture in cucumber assists the plants to realize a biological potential and to reach the highest productivity. The observed plant products are of high quality without pesticides and other harmful contaminants. The nitrate content in young fruits was significantly lower the threshold allowable concentration (TAC). The basic principles of intense technology of light-culture can be used for economically profitable enterprises for production of raw material needs for pharmaceutical and perfumery industries, and to produce vitamin products in small volumes for kindergardens, hospitals, schools and private housekeeping.
The description information on new high yield and large fruit tomato hybrids F1 ‘Altadena’ and ‘Bolena’ for prolonged rotation in glasshouses is given. Technology for their cultivation is presented in the article.
Humic compounds occupy the important place in the chain plant – water. Their application in technologies for vegetable plant growing with the use of drip irrigation enables to increase the coefficient of nutrient compound assimilation from soil with applied fertilizers. The preparation ‘Lighohumate’ presented in the article is integrated fertilizer, possessing the properties of anti-stress, immunomodulator and sticking agent. This preparation has the highest content of humic acids to 20% for liquid and to 90% for dried, consequently it has a powerful influence on plant organism, opening hidden biological reserves, and improving the crop productivity. The experimental tests showed that ‘Lighohumate’ application trough drip irrigation system together with foliage fertilizers had given significant yield augmentation; 5-20% depending on medium and technology in vegetable crops cultivated with different agricultural techniques. The permanent speeding-up from 3 to 12 days was noticed in the course of plant development with increase of early yield outcome. ‘Lighohumate’ can be used as seed treatment agent as well as foliage fertilizer and already applied giving a result in many vegetable production enterprises.
PLANTS PHYSIOLOGY AND PHYTOCHEMISTRY
Physalis (Physalis L.) is grown worldwide. P. pubescens L. is most widespread in Russia. The analysis of development of biochemical properties in fruits of P. pubescens L. in temperate climate was presented in the article. The berries of physalis have acid reaction and high nutrient value due to polyphenols, carotenoids that give orange color, also contain moderate quantity of vitamin C. Moreover in the berry juice there are significant quantities of potassium, phosphorus, calcium, magnesium, zinc, copper, iron, manganese and boron. P. pubescens L. is a promising exotic crop that can be used for production of functional foodstuffs. Physalis berries are usually used as fresh vegetables for salad recipes, providing the sweet and sour balanced taste. The whole fruit berries are also processed into syrups or dried, so that they are regarded as ‘elegant raisins’. Our study showed that the dry matter content was 15.5% in cultivar ‘Zolotaya Rossiyp’ in temperate climate, while the ascorbic acid content was 9.9 mg per 100 g. of wet weight. In temperate climate 318 mg GAE/100 g. polyphenols were accumulated in the berry of P. pubescens L. The titrated acidity of ‘Zolotaya Rossiyp’ berries was 0.90%, at pH 4.72. The level higher than 4 confirms the low acidity. This can be explained by the fact of presence of organic acids as a free forms in the berry juice.
Selenium deficiency is widespread among the human populations of different countries of the world and associating with cardiovascular diseases and cancer that determines the necessity to develop new technology needs to increase selenium accumulation levels by agricultural crops. Besides that, the utilization of selenium containing food supplements, soil and foliar application of selenium salts are the most popular approach to resolve the selenium deficiency problem. At the same time there is a certain drawback of selenium plant biofortification, which is a low accumulation of the element, and as a result, the possibility of environmental pollution caused the element. The review is devoted to a new direction in improving plant selenium accumulation via utilization of rhizobacteria; their participation in selenium cycle; mechanism of bacteria-selenium-plant interaction; the role of volatile compounds, released by rhizobacteria, on selenium accumulation. The questions that were separately considered were regarding rhizobacteria Microbacterium oxydans extracted from Cardamine hupingshanesis that had an exceptionally high resistance to selenium impact and ability to synthesize selenocystin.
In recent years much attention is given to the search for new medicinal plants and an elaboration of preparation from plant raw material that is used in ethnomedicine and for production of biologically active compounds (BAC). The forest herbaceous plants from Raifskiy district in Volgo-Kama National Park, and group of weed plants from urban and suburb places were exanimated for BAC content. In the group of herbaceous plants the flavonoid content varied from 1.10 to 15.85% in dry matter; the sum of chlorophylls content varied from 1.13 to 3.59 mg/g of dry matter. Among plant species exanimated: stone bramble, European wild ginger, eagle fern and white goosefoot were not regarded as pharmacopoeial plants, but they were characterized by significantly high content of flavonoids 2.74-4.26%. Flavonoid and chlorophyll contents in the tissues of observed plants changed during of process of ontogenesis in most species and were highest in the phase at the beginning of flowering and during flowering time. It was shown that antioxidant and flavonoids contents increased while manmade load rose as noticed in birdweed (Polygonum aviculare L.). Thus, the examined different species of herbaceous plants can be regarded as promising sources of BAC.
ECONOMICS AND MANAGEMENT OF NATIONAL ECONOMY (AGRICULTURE)
Economical efficiency of agricultural industry is a major characteristic of the level of development of an enterprise. A profit from product sale depends on volume and structure of product sales, self-cost as well as the level of sales price. Thus, the gross revenue from cultivated crops and vegetable cultivars can be observed at the time of fruit harvesting. The total sum of the gross earnings from the harvest, determining the efficiency of enterprise can be calculated as a product of values of daily price and mass of total harvest. There are no challenging points in the condition of permanent price and vegetable harvest. Even the registration of average-sales prices for vegetable doesn’t make the production difficult. But real market situation essentially differs from accepted practice to register average-sales prices with relatively permanent vegetable harvest. The price indexes of sales for tomato and cucumber produced in greenhouse in Udmurt Republic with showing the dynamic of retail price for vegetables were presented in the article. It was shown that prices of tomatoes and cucumbers had the seasonal factor that meant weekly price wavering. The temporal row harvest of cucumber ‘Tseres F1’ and tomato ‘Admiro F1’ produced at Zaviyalovskiy greenhouse enterprise was described. The average derivation between nearest and last harvest was in the gap 23% to 29% in cucumber and reached up to 70% in tomato. The values of such derivations were occasionally provoked and determined by some inter-enterprise factors. The construction of trend lines for such series and further line evaluation with the aid of determination coefficient R2 showed extremely low quality of model of the kind y=a•x+b. The value of R2 cubic polynominals was in the limits R2=0.32-0.46. Essentially, plans for vegetable production, constructed on the basis of this linear model should be regarded as unsatisfied. According to the analysis presented above, it is worth concluding that the principles to plan the production process in greenhouse enterprises have to be changed. As an example, the use of tk value as a shift of harvest time for kcrop related to price maximum for the crop used can be very effective.
ISSN 2618-7132 (Online)