Relevance. Maintaining the phytosanitary condition of vegetable and melon crops is an important component in the technologies of cultivation of new and promising varieties and hybrids, since losses from diseases of various etiologies reach 30% or more. This determines the need for immunological studies of the resistance of vegetable and melon crops to phytopathogens of various etiologies, where the basis of the strategy for creating new varieties and hybrids is the joint work of a breeder and a phytopathologist. To do this, it is important to conduct an annual study of the species composition in order to identify new harmful objects that have a negative impact on the profitability of production, as well as to develop measures to protect vegetable crops from the most harmful and economically significant pests and diseases.

Methodology and Results. The extensive experimental network of the Federal Scientific Vegetable Center (FSBSI FSVC) in various ecological and geographical zones allows testing varieties and hybrids of vegetable and melon crops in various soil and climatic conditions, including evaluating them for the most harmful phytopathogens in these regions. This review article briefly highlights the main stages of the history of development and current trends in phytopathological research in Russia and our center. The most significant achievements of scientists of the FSBSI FSVC are presented, aimed at a comprehensive study of the current state of populations of pathogens on vegetable and melon crops, resistance factors controlling the interaction of organisms in pathosystems, including genetic ones, optimization and development of new methodological approaches in breeding for immunity and scientific justification of recommended modern protection systems.

Relevance. Pea rust regularly damages the vegetable pea crop in the Omsk region. The harmfulness of the disease has begun to intensify in recent years; in years favorable for the development of the disease, the damage to susceptible varieties can be 100%. Therefore, breeders are forced to constantly search for new sources of resistance genes due to the rapidly developing rust pathogens. The goal of our research is to screen samples of a vegetable pea collection under field conditions in the southern forest-steppe of Western Siberia and identify sources of resistance.

Material and Methodology. As an object for study, 72 samples of vegetable peas were taken from the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC) and the “Federal Research Center of the All-Russian Institute of Plant Genetic Resources named after. N.I. Vavilov" (VIR). The research was conducted over four years from 2020 to 2023. in the conditions of the southern forest-steppe of the Omsk region. The records were carried out in accordance with the “Methodological instructions for studying the collection of grain legumes”.

Results and Discussion. As a result of the studies, it was established that the resistance of vegetable peas to rust depends on both the genotype and weather conditions. Weather conditions had the greatest influence on the development of the disease (the factor’s share was 51.5%), the contribution of the genotype was also significant and amounted to 44.8%. The resistance of collection samples to rust in the conditions of the Omsk region varied from low (2.8 points) in warm and humid weather to very high (7.8 points) in dry and hot weather. The main influence on the spread of rust is exerted by the hydrothermal conditions of the “flowering – ripening” period (r=-0.98±0.11). Plant resistance to rust varies greatly depending on growing conditions (Cve=20.2-54.6%). The variety Darunok had the least variability of the trait over the years (Cve=20.2%), which will most consistently maintain resistance when the infectious load increases. As a result of screening of collection material of vegetable peas, sources of partial resistance to rust were identified for breeding in Western Siberia: varieties Darunok, Namorodok (Federal Scientific Vegetable Center (FSBSI FSVC)), Orel, Cruiser, Vityaz, Flagman-8, Nemchinovsky 46, B-579, In Memory of Khangildin (VIR collection).

Relevance. Contarinia nasturtii Keiffer is one of the most dangerous insect pests of Brassicaceae plants dramatically affecting plant yield and seed productivity.

Material and Methods. The effect of single foliar application of sodium selenate, ionic form of silicon (Siliplant fertilizer) and garlic extract on the efficiency of Raphanus sativus lobo defense against Contarinia nasturtii infestation was evaluated in condition of greenhouse. Concentrations of the applied reagents were: sodium selenate 26.4 mM; Siliplant – 1 ml/l; garlic extract – 8 g/l. Seed productivity and C. nasturtii infestation levels under Se, Si and garlic extract administration under infestation were determined along with the analysis of plant antioxidant status and sugar content.

Results. Foliar application of Siliplant singular or in combination with Se provided the highest seed productivity and mass of 1000 seeds. Plants treated with Si, garlic extract and Si+Se demonstrated lack of C. nasturtii infestation only a week after the preparation supply. Contrary, selenate provided less pronounced defense. Among biochemical parameters tested the values of the total dissolved solids were the highest in plants treated with Se and Se+Si. Changes in the intensity of plant defense due to garlic, Se and Si supply were reflected in the increase of the total phenolic content compared to the values typical for control plants and in a significant accumulation increase of disaccharides, participating in tissue recovery after herbivory attack. Silicon application also provided an increase of selenium accumulation by lobo leaves.

Relevance. Cercospora leaf spot (Сercospora beticola) is a widespread disease of table beet that reduces the yield of root crops in many beet-growing regions. Annual losses from cercosporosis amount to 30-40%, and in the years of epiphytotic diseases – 50% or more. The fungus has a complex intraspecific structure, includes many pathotypes and races, which should be taken into account when breeding for resistance. The main method of creating resistant forms of table beet to C. beticola is the selection of cercospore-resistant lines in the natural manifestation of the disease and on artificial backgrounds. In open ground conditions, it is not always possible to assess the resistance of table beet to cercosporosis, since cercosporosis is weak in some hot and dry years. Therefore, laboratory assessment methods are important to accelerate the breeding process. The purpose of the research is to carry out an immunological analysis of beet lines in the field and laboratory conditions for the selection of sustainable material.

Materials and methods. The assessment of beet variety samples for resistance to cercospora blight was carried out in open ground conditions on the basis of the All-Russian Scientific Research Institute of Vegetable Growing – branch of the FSBSI Federal Scientific Vegetable Center (Moscow region). In laboratory conditions, artificial infection of separated red beet leaves was carried out. The objects of the study were inbred lines of red beets of the 1st-6th generation.

Results and discussion. As a result of evaluation and selection, red beet lines No. 713 and 790 with increased resistance to cercospora blight were identified. It has been established that for practical breeding it is necessary to constantly and strictly select for resistance to cercospora blight. In laboratory conditions, a method of artificial infection of separated leaves has been developed, which allows for a short period of time to carry out a preliminary assessment of a large number of beetroot samples for resistance to cercospora blight.

Relevance. Eating a natural product of seedlings can completely replenish the body with missing vitamins, but there are problems in obtaining seedlings – damage to seeds by a complex of phytopathogens. Obtaining high-quality soy seedlings is an urgent task.

The purpose of the research is to identify the main problems associated with the manifestation and removal of pathogenic microbiota in the production of vegetable soybean seedlings.

Material and Methods. The seeds of domestic and foreign soybean varieties served as the material. Patent No. 2349098 of the Russian Federation. The determination of germination and infection of seeds was carried out according to GOST 12038-84 in 4 repetitions. Methods of disinfection of seeds: ultraviolet radiation and ozonation.

Results and Discussion. It was revealed that the Hidaka variety has a high seed contamination of 48.7%, and low germination. The varieties Sibiryachka and Lydia have high germination, pathogen infestation is 6 and 8%, respectively. The contamination of the seeds of the Bar variety was a minimum of 2.7%, this variety was taken into further work. Pathogens from the genera Mucor spp., Fusarium spp., Alternaria spp., Pythium spp., Penicillium have been isolated. The phases of water consumption of vegetable soybeans have been determined, which makes it possible to determine the weight of the seed in a specific period of time and use the data for pre-sowing seed treatment. Ecological methods of disinfection of seed material have been tested – treatment with ultraviolet radiation, the use of ozone washing and ozonation of water. An analysis of the percentage of the yield of healthy and affected seedlings, depending on the method of seed treatment, showed that the untreated option has the lowest number of healthy seedlings – 10.76%. UV treatment of dry seeds and treatment in an ozone sink allowed to increase the yield of healthy seedlings to 14.24 and 14.30%, respectively. UV treatment of soaked seeds allowed to increase the yield of healthy products by 62%, and the option of using an ozone sink with further use of ozonated water allowed to obtain the maximum yield of healthy seedlings – 20.04%, which is 2 times higher relative to the control.

Relevance. Large differences in the climatic conditions by the region Altai by years determine the prevalence and harmfulness of diseases, therefore, monitoring by the dynamics of disease development and assessing their harmfulness are relevant and necessary measures. An important role in modern plant protection technologies against diseases play biofungicides. The main advantages of biofungicides as compared with chemical plant protection products are safety for humans and the environment, short waiting times and the possibility of use throughout the growing season, the absence by risks of the emergence of resistant pathogens, the creation of conditions for the natural self–regulation of agrocenoses.

Materials and Methods. The research was carried out in 2018-2020 on the fields of the West Siberian Vegetable Experimental Station of the branch of the West-Siberian vegetable experimental station – branch of the Federal State Budgetary Scientific Institution «Federal Scientific Vegetable Center». In the experiment was used the F1 hybrid Nezhinsky of Siberia. Accounting of the phytosanitary condition of crops were carried out in three periods with the determination by the degree of spread and the degree of development of the disease. Studied were the doses and timing of the use of biological preparations Phytolavin and Phytoplasmin in the fight against cucumber bacteriosis during the growing season. The repeatability of the experience was 4-fold, the placement of plots in 4 tiers, the area of the plot was 10 m² , the accounting area was 3 m² . The total area were 0.05 hectares.

Results. The applicationof drugs Phytolavin (2 l/ha), Phytoplasmin (3 l/ha), promotes to reduce the pace of development of development of bacterial spotting of cucumber in the open ground. The least development of the disease was noted by using the drugs Phytolavin 2 l/ha, Phytoplasmin 3 l/ha, Phytolavin 2 l/ha, with alternation and an interval of 14 days. By the influence of bactericidal treatments against bacterial spotting of cucumber, a higher weight of seed fruits was obtained in all variants compared to the control. The best result – 11.96% to the control was obtained on the variant by using the preparations Phytolavin 2 l/ha, Phytoplasmin 3 l/ha with alternation.

Relevance. Cabbage Kale (Brassica oleracea L. var. acephala) is a green, red and purple leafy vegetable cultivated in Northern and Central Europe, as well as North America. Kale deservedly occupies a high place in the list of functional products due to its high content of biologically active substances. Kale contains vitamins, minerals, antioxidant compounds, carbohydrates, unsaturated fatty acids, proteins and dietary fiber. Despite its high attractiveness as a functional food product, it is not cultivated on a massive scale in Russia.

Methodology. The purpose of this work is to study the content of phenolic compounds in two varieties of kale cabbage to substantiate recommendations for its cultivation in Russia. The variety and hybrid of kale cabbage – Dwarf Blue Scotch with green leaves and the hybrid Redbor F₁ with red leaves – were used as the object of the study. The content of soluble phenolic compounds and flavonoids, as well as the qualitative composition of phenolic compounds in kale cabbage, was studied. Determination was carried out according to generally accepted methods. Grown plants at the age of 16 weeks were used as samples for analysis. The experiment was carried out in laboratory conditions in 5 replicates.

Results. The results of the studies indicate a fairly high content of phenolic compounds and flavonoids in the studied varieties of kale cabbage. It has been demonstrated that the Redbor F₁ hybrid is characterized by a higher content of soluble phenolic compounds compared to the Dwarf Blue Scotch variety by an average of ~20%. It was concluded that this Redbor F₁ hybrid can be used to develop methods for increasing the efficiency of the synthesis of secondary metabolites that are beneficial to human health, and is recommended to farmers for cultivation in the Russian Federation.

Relevance. Rhodiola rosea L. is a perennial herbaceous plant from the Crassulaceae family. The roots and rhizomes of R. rosea contain a range of biologically active compounds. This species is included in the State Pharmacopoeia of the Russian Federation. Medicinal preparations derived from them are widely used as adaptogens, general tonic and anti-stress agents. While the roots have been extensively studied, less attention has been given to the biologically active compounds found in the leaves of R. rosea. An introduced population of R. rosea grows on the territory of the botanical garden of medicinal plants of the All-Russian scientific research Institute of medicinal and aromatic plants (VILAR). The possibility of cultivating this species in the conditions of the Moscow region is being studied.

Material and Methodology. The objective of this study was to investigate the composition of phenolic compounds in R. rosea leaves from VILAR biocollection using ultra-efficient liquid chromatography combined with mass spectrometry. Extraction was carried out with 80% acetone. An ultra-high performance liquid chromatographic system with a photodiode array detector and mass spectrometer was used for the analysis of phenolic compounds. Identification was carried out using UV- and mass spectrometry data.

Results. As a result of UPLC-DD-MS analysis, peaks of 20 phenolic compounds were detected in R. rosea leaves, of which 19 compounds were tentatively identified. The leaf extract contained eight phenolic compounds from the group of galloyl-glucoses (three isomers of monogalloyl-glucose, trigalloyl-glucose, two isomers of tetragalloyl-glucose, two isomers of pentagalloyl-glucose), two gallotannins (isomers of hexagalloyl-glucose), five flavonoids (four herbacetin derivatives and one gossypetin), phenolic acids (caffeoyl-malic and ferulic acids), arbutin and esculetin. It has been shown that the biologically active substances of R. rosea provide prospects for the use of leaves as raw materials for the development of herbal preparations with anti-inflammatory, antiviral and antitumor effects.

Relevance.Medicinal sage is a pharmacopoeial species, a valuable essential oil plant. The method of plantation establishment is predominantly seed-based, and the seed material can retain pathogenic microbiota, so the purpose of the study was to identify mycosis pathogens in the seeds of medicinal sage.

Materials and methods. The study was carried out on samples from the biological collection of Salvia officinalis seeds (as part of the Unique Scientific Installation “Biological Collections of the All-Russian Research Institute of Medicinal and Aromatic Plants”). Seeds of the 1^st–5^th and 9th years of storage were collected from the nurseries of the Experimental Field of the All-Russian Research Institute of Medicinal and Aromatic Plants. Research to identify mycoses of sage seeds was carried out at the All-Russian Research Institute of Phytopathology in the innovative laboratory of microbiology. To study the phytopathogens of Salvia officinalis seeds, the method of artificial nutrient media was used.

Results. From the study conducted, the effect of predominant pathogen was established. Fungi of the genus Alternaria, when seeds are stored in conditions with a temperature regime of +20...+22℃, have a negative effect on the sowing quality of seeds. Laboratory germination and germination energy of seeds stored in the cold room (0...+5℃) were affected by yeast, a significant decrease in sowing qualities was observed. When seeds were stored in -18℃, the inhibitory effect of temperature on pathogens was found. The dependence of total pathogen infestation on the conditions and term of seed storage was established. The highest percentage of infestation in seeds stored at room temperature was observed in the first 2 years from the time of collection. The development of pathogenic fungi of Alternaria, Phoma and Rhizopus genus was significantly reduced in seeds stored for 5 to 9 years with temperature conditions of 0...+5℃ and -18℃. Rhizopus, Cladosporium cladosporioides and Clonostachys rosea were found in the sample stored for 38 years in the freezer. The dependence of the set of pathogenic microbiota of seeds on the predecessor in the crop rotation was established. The plots from which seeds were collected, except for the sample from the Crimean zonal experimental station of VILR, have different predecessors. The seeds from the first plot showed the highest percentage of fungal infestation with similar pathogen composition. Total infestation and pathogen composition decreased in the second plot. The sample from the third plot was stored for 5 years in the freezer and no seed mycoses were detected in it.

Relevance. In the transition to highly productive and environmentally friendly, in particular, organic agriculture and production of safe and high-quality products, which is a priority direction of strategies of scientific and technological development of the Russian Federation, one of the necessary links is the creation and control of optimal conditions of plant nutrition, i.e. provision of plants with nutrients in the right combination throughout the vegetation. Achievement of such conditions is possible through soil and plant nutrition diagnostics, which in turn allows to establish the ability of crops to assimilate nutrients from the soil and adjust the doses of mineral fertilisers as top dressings.

Material and Methods. On alluvial meadow soil in the conditions of the non-Black Earth region of Russia, the effectiveness of root feeding with macronutrients (NPK) was studied according to the results of plant and soil diagnostics of table root crops nutrition when grown on organic and mineral fertiliser systems. Plant and soil diagnostics of mineral nutrition was carried out according to the methods of Zerling V.V. (1990) and Magnitsky K.P. (1972). Two crops were tested during the experiment: red beet variety Mulatka and garden carrot variety Losinoostrovskaya 13.

Results and Discussion. Root fertilizers on the basis of nutrition diagnostics on the basis of soil and leaf petiole analysis made significant changes in the dynamics of basic nutrients in the soil, there was an increase in nitrate nitrogen and mobile potassium in comparison with the control variant, with natural soil supply of mobile phosphorus (more than 250 mg/kg), which had a positive effect on the root nutrition of plants. At the main application of mineral and organic (biocompost BIUD) fertilizers, root fertilizers according to nutrition diagnostics the carrot yield 51,2-63,9 t/ha with standard 87,4- 94,6%, red beet 41,7-54,2 t/ha with standard 80,7-92,3% were obtained. Root feeding of carrots by soil diagnostics N₅₂K₁₄ on the background of biocompost gave 24.9% increase to the control, by plant diagnostics N₁₇ gave 16.6%; red beet by soil diagnostics N₆₅K₄₅ – 30% increase, by plant diagnostics N₅₄K₃₀ – 23.7%

Relevance. Due to climate change on the planet, it is becoming increasingly difficult to obtain high yields of melons and melons in rain-fed conditions. Therefore, it is necessary to develop modern methods of growing them. Accordingly, the use of new water-soluble fertilizers for the cultivation of melon in hot climates is a relevant work at the moment.

Material and methodology. Objects of research: medium-ripening melon variety GP 599f and watersoluble fertilizers Lignohumate, Energen Extra, Zinc Sulfate. We have carefully studied options using these fertilizers for double foliar treatment of plants during the growing season.

Results. As a result of studying new types of water-soluble fertilizers on increasing the vegetative mass of plants, yield and biochemical parameters of the variety sample, a positive effect was revealed. As a result of a comparative analysis of data on plant development after double treatment with the studied preparations, an increase in canes in all variants was noted in relation to the control by 4.3-18.7%. When calculating the yield in all studied options, an increase in these indicators was noted in relation to the option without treatments. After treating the plants with new types of fertilizers, the yield was 13.4-15.9 t/ha. The average fetal weight varied from 1.7 kg. up to 1.8 kg with maximum values in the Lignohumate and Energen Extra options. An analysis of the comparison of the biochemical composition of fruits showed that new types of fertilizers did not affect the taste and purity of the products. The dry matter content was noted to be 0.8-1% higher than the control variant. Nitrate levels in fruits did not exceed the maximum permissible concentration (90 mg/kg) and amounted to 24-28 mg/kg.

Relevance. Grain of vegetable pea varieties is processed at canning factories, frozen, dried, and consumed fresh. Industrial cultivation of peas can be made more environmentally friendly by using biological preparations that contain growth-stimulating bacteria, for example Rhizobium species. With additional inoculation with rhizobacteria, legumes have higher values of traits characterizing vegetative development, biochemical parameters and yield; their immunity and resistance to abiotic stress increases as well.

Methodology. The aim of our study was to evaluate the effect of additional inoculation of Rhizobium leguminosarum strain 245a on biometric and physiological parameters and yield of vegetable pea varieties. The experiment was carried out at Krymsk Experiment Breeding Station – a branch of VIR (Krasnodar Region, Krymsk). We studied six commercial cultivar: Prima, Mayak, Alpha 2, Vesta, Parus (leafless morphotype) and Patriot. The seeds were treated the day before sowing with an aqueous solution of the biopreparation Rhizotorfin (produced by ECOS BIOPREPARATIONS, St.Petersburg) and with water in the control variant.

Results. The use of the preparation had a growth-stimulating effect on vegetable pea plants. An increase in the length of plants, the height of attachment of the lower pod and the number of completed pods on the plant was found. An increase in the content of dry matter in the total biomass of plants, stems with leaves and pods was noted when using Rhizotorfin. Also, inoculation with rhizobacteria contributed to an increase in yield. The effectiveness of the biopreparation depended on the conditions of the year. The maximum difference between the values of the studied traits for the control and treatment variants was in 2023 - with a uniform distribution of precipitation and optimal temperatures during the growing season. A genotype-dependent reaction of vegetable pea plants to seed treatment with bacteria Rhizobium leguminosarum strain 245a was revealed. The most responsive to additional inoculation is the variety Parus of the leafless morphotype. The values of most biometric and physiological traits and yield in this genotype were significantly higher in the variant with the use of the Rhizotorfin biopreparation. Based on the results obtained, we recommend using the Rhizotorfin biopreparation when growing the variety Parus for commercial production.

Relevance. Oenothera tetragona is an ornamental plant used in folk medicine. Until now, studies of the biological characteristics of the Oenothera tetragona have not been carried out on the territory of the NonChernozem zone of the Russian Federation.

The purpose of the work is the introduction and biological study of the Oenothera tetragona in the NonChernozem zone of Russia in the conditions of the Moscow region.

Methodology. The experiment was based on two-month-old seedlings according to a 60x30 cm pattern. Phenological observations and biometric measurements (during the mass flowering phase) were carried out according to the methods adopted at VILAR (All-Russian scientific research Institute of medicinal and aromatic plants). Experimental data were processed according to B.A. Dospehov. Weather conditions were checked on the Weather and Climate website. Microspecimens were prepared according to methods for light microscopy.

Results. In the conditions of the Moscow region the period from the beginning of the growing season to mass fruiting in Oenothera tetragona Roth. averages about 110-115 days. The plant is well adapted: it goes through the entire seasonal cycle of growth and development. The population includes Oenothera tetragona Roth. includes many variable biotypes, which indicates the possibility of targeted selection of forms with useful traits. The main assessment of breeding material can be carried out, starting from the second year of plant life, based on a set of interrelated quantitative traits (height, number of generative shoots, etc.). Some anatomical features of the epidermis of the Oenothera tetragona leaf have been studied for the first time; the number of covering trichomes and the number of stomata per 1 mm² were determined. As a result of microscopic examination of the epidermis of leaf plates of O. tetragona Roth.: the presence of three groups was revealed, which differed in microscopic characteristics (shape and number of stomata, pubescence) in the absence of a noticeable correlation with the main phenotypic characteristics.

Relevance. Vegetable soybean is a special variety with high added value, consumed fresh and harvested at the stage technical ripeness (R6). Accession-A is one of the promising vegetable soybean materials, studied and identified as a result of work that was carried out jointly with the Federal Scientific Vegetable Center in the Moscow Region (FSVC) and the Agrobiotechnology Department of the Agricultural Technological Institute (ATI) of the RUDN University named after Patrice Lumumba. The use of microorganisms to promote atmospheric nitrogen fixation is a common practice in soybean production.

Methodology. The purpose of this study was to evaluate the effect of two microbiological preparations based on Sinorhizobium fredii and Bradyrhizobium jaроniсum in order to identify which microbiological preparation gives the best results in terms of morphological indicators and vegetable soybean yield under the conditions region of the Non-Chernozem zone of the Russian Federation. The object of the study was accession-A of vegetable soybean. Two types of treatment were carried out: B1 – treatment with a preparation with Bradyrhizobium jaronicum (1 l/t of seeds), B2 – treatment with a preparation with Sinorhizobium fredii (1 l/t of seeds). Control without treatment (K).

Results. According to statistical analysis of biometric indicators, it was found that treatment with S. fredii (B2) had the highest average values for indicators of stem diameter (11.50 mm), lower pod attachment height (11.19 cm), number of branches (3.40 pcs.) and the number of productive nodes (27.48 pcs.). Preparation B2 improved the structure of the crop, influencing the obtainment of the highest indicators of plant weight (30.47 g), seed weight per plant (15.33 g), weight of 1000 seeds (171.25 g), number of seeds per plant (98.50 pcs.), number of seeds in a bean (2.89 pcs.) and seed yield (3.11 t/ha). The use of microbiological preparations based on B. japonicum and S. fredii gave better results compared to the control treatment.

Relevance. For the production of pharmaceutical products from raw materials of Echinacea purpurea L., it is necessary to have a sufficient amount of high-quality seeds when planting production areas with high yields of aboveground mass.

Methods. On Echinacea purpurea of the second year of vegetation in order to increase herbage yield and seed productivity, tests of organomineral fertilizer EcoFus (1.5 l/ha) with microfertilizers (Siliplant (0.5 l/ha) or Cytovit (1.0 l/ha). As a standard, we used double foliar feeding with Siliplant (0.5 l/ha). The above-ground mass was mowed in the third ten days of August. On seed plantations we tested: in the early growth phase EcoFus (1.5 l/ha), in the stemming phase – growth regulators Epin-extra (0.06 l/ha), Agat-25K (0.04 l/ha) and their tank mixtures. Seed collection was carried out in mid-September.

Results. Double spraying with complex – EcoFus with microfertilizers, promoted activation of growth processes, and it resulted in 22-26% increase of above-ground mass yield, 5-7% increase of phenylpropanoids in it, their collection per hectare increased within 28-35%. A more effective complex was revealed: EcoFus (1.5 l/ha) with Siliplant (0.5 l/ha), which allowed to increase yield and collection of active substances by 26 and 35% in comparison with the control, and in comparison with the reference (Siliplant 0.5 l/ha) – by 11 and 15%, respectively. In order to increase seed productivity of Echinacea purpurea, spraying with EcoFus at the beginning of vegetation of plants and EcoFus complex with bioregulators Agat and Epin-extra in the phase of stemming was applied. Foliar fertilization with these tank mixtures resulted in earlier flowering of plants (by 2-4 days), increased the number of inflorescences by 9-10%, inflorescences with seeds by 14-15% and seed weight of one inflorescence by 12- 14%. Activation of generative processes of Echinacea purpurea had a positive effect on seed yield and their quality: yield increased by 21-25%, weight of 1000 pieces by 7-8% and their germination by 2-3%.

Relevance. Agroforestry systems are believed to provide a multitude of ecological services. It is thought that agroforestry enhances resilience to the impacts of climate change and aids in adaptation by supporting diverse land use practices, sustainable lifestyles, and income streams, as well as increasing productivity in both forests and agriculture, and reducing weather-related losses in production.

Results and Discussion. The aim of this review was to present genuine evidence on the role of agroforestry in ecosystem conservation and mitigation of climate change impacts. Compared to monocropping and open cereal-based agriculture, agroforestry has made a more significant contribution to ecosystem conservation and in reducing carbon dioxide emissions. However, it has been found that agroforestry contributes less to carbon sequestration than natural forests. Carbon sequestration through above-ground and underground biomass, carbon emission reduction from deforestation, and microclimate adjustment are key measures for mitigating climate change. Agroforestry systems provide essential ecosystem services, such as food, fuel wood, fodder, income, and improved soil production, which enable communities to cope better with the impacts of climate change. Therefore, agroforestry must be given significant attention if it is to play a crucial role in ecosystem management.