Preview

Vegetable crops of Russia

Advanced search

DETERMINATION OF CYTOPLASMIC MALE STERILE FACTORS IN ONION PLANTS ( ALLIUM CEPA L.) OF VNIISSOK'S BREEDING

https://doi.org/10.18619/2072-9146-2011-4-20-21

Abstract

Molecular markers were used for screening of the onion plants of VNIISSOK's breeding for cytoplasmic male sterility. Allelic variations of the mitochondrial genes orfА501 and cob were reveled among studied onion samples. Use of the PCR-markers for these genes allowed identifying the cytoplasmic types of 14 onion genotypes. Application of these molecular markers in breeding program could help to select genotypes wit certain type of cytoplasm and may be useful for high throughput identification of cytoplasmic male sterile factors in large onion breeding populations.

About the Authors

T.P. Suprunova
State Institution, All-Russian Research Institute of Vegetable Breeding and Seed Production, RAAS
Russian Federation


A.N. Logunov
State Institution, All-Russian Research Institute of Vegetable Breeding and Seed Production, RAAS
Russian Federation


V.V. Logunova
State Institution, All-Russian Research Institute of Vegetable Breeding and Seed Production, RAAS
Russian Federation


A.F. Agafonov
State Institution, All-Russian Research Institute of Vegetable Breeding and Seed Production, RAAS
Russian Federation


References

1. Berninger E. Contribution a l'?dute de la sterililite' male de l'oignon (Allium cepa L.). // Ann. Amelior. Plant. - 1965. - V. 15. - P. 183-199.

2. Cho K-S., Yang T-J., Hong S-Y., Kwon Y-S., Woo J-G., Park H-G. Determination of cytoplasmic mail sterile factors in onion plants (Allium cepa L.) using PCR-RFLP and SNP markers. // Mol.Cells. - 2006.- V. 21.- No.3. - P. 411-417.

3. Engelke T., Terefe D., Tatlioglu T. A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L.). // Theor. Appl. Genet. - 2003. - V.107. P. 162-167.

4. Edwards K., Johnstone C., and Thompson C. A simple and rapid method for the preparation of genomic plant DNA for pCr analysis. // Nucleic Acids Res. - 1991. - V. 19. - P. 1349.

5. Havey M.J. Diversity among male-sterility-inducing and male-fertile cytoplasms of onion. // Theor. Appl. Genet. - 2000. - V. 101. - P. 778-782.

6. Jones H.A., Emsweller S.L. A male-sterile onion. // Proc.Amer.Soc.Hort.Sci.-1963. - V.34. - P. 582-585.

7. Jones H.A. and Clarke A. Inheritance of male sterility in the onion and the production of hybrid seed. // Proc.Amer.Soc.Hort.Sci. - 1943. - V. 43. - P. 189-194.

8. Kim S., Lee E.T., Cho D.Y., Han T., Bang H., Patil B.S., Ahn Y.K., Yoon M.K. Identification of a novel chimeric gene, orf725, and its use in development of a molecular marker for distinguishing among three cytoplasm types in onion (Allium cepa L). // Theor. Appl. Genet. - 2009. - V. 118. - P. 433-441.

9. Sato Y. PCR amplification of CMS-specific mitochondrial nucleotide sequences to identify cytoplasmic genotypes of onion (Allium cepa L.). // Theor. Appl. Genet. - 1998. - V. 96. - P. 367-370.

10. Yamashita K., Tsukazaki H., Kojima A., Ohara T., Wako T. Inheritance mode of male sterility in bunching onion (Allium fistulosum L.) accessions. // Euphytica. -2010. - V. 173. - P. 357-367.


Review

For citations:


Suprunova T., Logunov A., Logunova V., Agafonov A. DETERMINATION OF CYTOPLASMIC MALE STERILE FACTORS IN ONION PLANTS ( ALLIUM CEPA L.) OF VNIISSOK'S BREEDING. Vegetable crops of Russia. 2011;(4):20-21. (In Russ.) https://doi.org/10.18619/2072-9146-2011-4-20-21

Views: 399


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2072-9146 (Print)
ISSN 2618-7132 (Online)