ovoshchiОвощи РоссииVegetable crops of Russia2072-91462618-7132Федеральный научный центр овощеводства10.18619/2072-9146-2011-4-20-21ovoshchi-1641Research ArticleСОВРЕМЕННЫЕ НАПРАВЛЕНИЯ СЕЛЕКЦИИ ОВОЩНЫХ И КУЛЬТУРADVANCED DIRECTIONS IN VEGETABLE PLANTS BREEDINGОПРЕДЕЛЕНИЕ ТИПА ЦИТОПЛАЗМАТИЧЕСКОЙ МУЖСКОЙ СТЕРИЛЬНОСТИ ЛУКА РЕПЧАТОГО ( ALLIUM CEPA L.) СЕЛЕКЦИИ ВНИИССОК С ПОМОЩЬЮ МОЛЕКУЛЯРНЫХ МАРКЕРОВDETERMINATION OF CYTOPLASMIC MALE STERILE FACTORS IN ONION PLANTS ( ALLIUM CEPA L.) OF VNIISSOK'S BREEDINGСупруноваТ.П.SuprunovaT.P.vniissok@mail.ruЛогуновА.Н.LogunovA.N.noemail@neicon.ruЛогуноваВ.В.LogunovaV.V.noemail@neicon.ruАгафоновА.Ф.AgafonovA.F.noemail@neicon.ruГНУ Всероссийский НИИ селекции и семеноводства овощных культур РоссельхозакадемииРоссияState Institution, All-Russian Research Institute of Vegetable Breeding and Seed Production, RAASRussian Federation201130122011042021Copyright © Супрунова .Т., Логунов .А., Логунова .В., Агафонов .А., 20112011Супрунова .Т., Логунов .А., Логунова .В., Агафонов .А.Супрунова .Т., Логунов .А., Логунова .В., Агафонов .А.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vegetables.su/jour/article/view/1641

Впервые проведена оценка отечественных сортообразцов лука репчатого с помощью молекулярных маркеров на признак ЦМС. Выявлен полиморфизм по митохондриальным генам orfА501 и cob. Определены типы цитоплазмы у всех изученных образцов, что в дальнейшем позволит селекционерам сократить время, необходимое для отбора генотипов с определенным типом цитоплазмы и проведения скрещиваний.

Molecular markers were used for screening of the onion plants of VNIISSOK's breeding for cytoplasmic male sterility. Allelic variations of the mitochondrial genes orfА501 and cob were reveled among studied onion samples. Use of the PCR-markers for these genes allowed identifying the cytoplasmic types of 14 onion genotypes. Application of these molecular markers in breeding program could help to select genotypes wit certain type of cytoplasm and may be useful for high throughput identification of cytoplasmic male sterile factors in large onion breeding populations.

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The authors declare that there are no conflicts of interest present.