Comparative evaluation of several Dynamics of accumulation of lactic and acetic acids in the process of directed fermentation of white cabbage of the Parus variety

Relevance and methodology. Today, white cabbage is the most popular type of vegetable raw materials used for fermentation not only in Russia, but also in other countries. To obtain a variety of flavors and shades of flavor, various vegetables (carrots, sweet peppers), fruit (apples, cranberries) and spicy-aromatic additives (cumin, dill seed, etc.) are added to the cabbage during fermentation. During fermentation, the product contains not only the initial components of the raw material (vitamin C, macro-and micronutrients), but also significantly increases the number of lactic acid microorganisms – the main "participants" in the process.
One of the main products of the metabolism of lactic acid microorganisms is lactic acid. At a concentration of 0.5%, it begins to inhibit the development of many microorganisms. When reaching a concentration of 1-2% microbiological enzymatic hydrolysis, as a rule, ceases. The accumulation of acetic acid allows us to judge the intensity of the heterofermentative stage of fermentation using strains of lactic acid microorganisms.

Results. The L. plantarum + L. casei consortium has shown a synergistic interaction in the accumulation of lactic acid, since the amount of acid produced by the consortium significantly exceeds the amount of acid produced by each strain separately. This point is also noted for the accumulation of acetic acid. In the L. brevis + L. casei consortium, the opposite situation is observed: when using cultures separately, the accumulation of lactic acid occurs to a greater extent than when using two cultures simultaneously. There are no obvious differences between monocultures and the consortium in terms of acetic acid accumulation. In the case of the L. plantarum + L. brevis consortium, we observe a synergistic interaction, with the dominant role in this consortium played by L. plantarum, and the contribution of L. brevis to the accumulation of lactic acid is insignificant. At the same time, the total resulting accumulation of acetic acid is significantly less than the result of each strain separately, which may indicate a negative mutual effect of the participants in the process. Acid degradation of the polysaccharide matrix of cell walls does not occur during the entire fermentation process.

1. Biotechnology of plant products: a textbook. Saint Petersburg: LAN publishing house, 2019. 232 p. (In Russ.)

2. Guizani N., Mothershaw A. Fermentation. In: Handbook of Food Science, Technology and Engineering. Hui Y H, editor. 2006;(2). Boca Raton: CRC Press. P. 63. (In Russ.)

3. Lactic acid fermentation. Wikipedia. [2007—2019]. Updated: 26.05.2019. URL: https://ru.wikipedia.org/?oldid=100051390 (accessed 26.11.2019). (In Russ.)

4. Kuranova N.G., Kupatadze G.A. Microbiology. Part 2. The metabolism of prokaryotes. M., 2017. 100 p. (In Russ.)

5. Saravacos G., Kostaropoulos A.E. Design of food processes and food processing plants. In: Handbook of Food Processing Equipment. Springer, Boston, MA. 2016:1-50. DOI: https://doi.org/10.1007/978-3-319-25020-5 (In Russ.)

6. Osipyan B. A. the Effectiveness of the use of biologics based on heterofermentative lactic acid bacteria in the silage of corn and herbs / abstract of the dissertation for the degree of candidate of agricultural Sciences. Moscow, 2018. 23 p. (In Russ.)

7. Caplice E., Fitzgerald G.F. Food fermentations: role of microorganisms in food production and preservation. Int J Food Microbiol. 1999;50(1-2):131-149. DOI: http://dx.doi.org/10.1016/s0168-1605(99)00082-3 (In Russ.)

8. Passport for the strain Leuconostoc mesenteroides subsp mesenteroides 37Y issued by the all-Russian collection Of industrial Microorganisms FSUE Gosniigenetika, date of issue 01 12 2016. (In Russ.)

9. Passport for the Lactobacillus brevis B-1309 strain, obtained at the allRussian research Institute of food biotechnology a branch of the Federal state budgetary institution of science of the Federal research center for nutrition, biotechnology and food safety , date of issue 28 05 2019 (In Russ.)

10. Passport for the Lactobacillus casei 536/17 strain obtained at the allRussian research Institute of food biotechnology – branch of the Federal state budgetary institution "FITZ nutrition and biotechnology", date of issue 08 04 2019 (In Russ.)

11. Passport for the strain of Lactobacillus plantarum 578/26, obtained at the all-Russian research Institute of food biotechnology – branch of the Federal state budgetary INSTITUTION "FITZ nutrition and biotechnology", date of issue 08 04 2019 (In Russ.)

12. Method of LLC "Lumex" M 04-47-2012 "method of measuring the mass concentration of organic acids and their salts". (In Russ.)