Selection of Cucumis sativus L. for resistance to fusarium wilt using filtrate of the culture fluid of the fungus Fusarium oxysporum Schlectend
Alexey V. Soldatenko,
Anna A. Egorova,
Olga V. Baklanova,
Alexander N. Hovrin,
Lyubov A. Chistyakovа,
Oleg A. Razin https://doi.org/10.18619/2072-9146-2019-4-50-53
Abstract
Relevance
Traditional breeding methods are based on crossing and selection of genotypes among hybrid offspring. In recent decades, along with traditional methods, more and more attention is paid to alternative methods of selection, based on biotechnological manipulations with plants. One of the most important methods of biotechnology is the method of cell selection, which is based on the replacement of the whole plant, as a unit of selection, on its cell. Applying biotechnology techniques from a single plant can produce millions of cells, which increases the chances of finding, eliminating the need for areas for the cultivation of tested plants. As well as accelerating the selection process due to the possibility to carry out the study in the offseason.
Methods
The studies used the linear material of C. sativus hybrids of All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center and Agroholding "Poisk". Plants were cultivated in laboratory room conditions. As explants used hypocotyl 0.5-1 cm segments isolated from young plants.
Results
To obtain Cucumis sativus plants with increased resistance to Fusarium by cell selection method, it is recommended to alternate culturing of callus on a non – selective medium containing sucrose in a concentration of 30 g/l, agar – 7 g/l, 0.1 mg/l, NUC – 0.5 mg/l and the filter of the cultural fluid of the fungus in a concentration of 10% within 3 passages.
About the Authors
Alexey V. Soldatenko
FSBSI Federal Scientific Vegetable Center
Russian Federation
Russian Federation
doctor of agricultural Sciences, Professor of the Russian Academy of Sciences, chief researcher
Selectionnaya str., 14, p. VNIISSOK, Odintsovo district, Moscow region, 143072
Anna A. Egorova
All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center
Russian Federation
Russian Federation
PhD, senior researcher of Solanaceae crops Immunity and Breeding
Vereya, Ramenskoye district, Moscow region, 140153
Olga V. Baklanova
All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center
Russian Federation
Russian Federation
PhD, leading researcher of breeding of cucurbitaceous crops laboratory
Vereya, Ramenskoye district, Moscow region, 140153
Alexander N. Hovrin
All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center
Russian Federation
Russian Federation
PhD, associate professor, head of department of breeding and seed growing
Vereya, Ramenskoye district, Moscow region, 140153
Lyubov A. Chistyakovа
All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center
Russian Federation
Russian Federation
PhD, senior researcher of breeding of cucurbitaceous crops laboratory
Vereya, Ramenskoye district, Moscow region, 140153
Oleg A. Razin
FSBSI Federal Scientific Vegetable Center
Russian Federation
Russian Federation
candidate of agricultural Sciences, senior researcher
Selectionnaya str., 14, p. VNIISSOK, Odintsovo district, Moscow region, 143072
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Review
For citations:
Soldatenko A.V., Egorova A.A., Baklanova O.V., Hovrin A.N., Chistyakovа L.A., Razin O.A. Selection of Cucumis sativus L. for resistance to fusarium wilt using filtrate of the culture fluid of the fungus Fusarium oxysporum Schlectend. Vegetable crops of Russia. 2019;(4):50-53. (In Russ.) https://doi.org/10.18619/2072-9146-2019-4-50-53
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