DEVELOPMENT OF ABNORMAL FLOWERS IN GENERATION OF DOUBLED HAPLOIDS OF SUMMER SQUASH (CUCURBITA PEPO L.)
https://doi.org/10.18619/2072-9146-2018-5-13-17
Abstract
The culture of unpollinated ovules in vitro in summer squash was used to develop fully homozygous breeding lines with the aim of the speeding-up breeding program. As a result of assessment for economically valuable traits, the seven promising DH-lines obtained from summer squash accessions differed by fruit shapes and colours were selected out. All breeding lines produced showed high homogeneity that retained in following generations and also have an appropriate set of economically valuable traits. DH-lines belonging to female type have up to 96% female flowers and only 4% male flowers. It is very important for breeding when the male flowers appeared in two weeks just after the female flower began blooming. The development of morphologically abnormal female and male flowers, along with gynandromorphy flowers was noted on selected DH-lines. During vegetation period from 26 to 36 flowers appeared on the plant, where out of them 19-21 ones were normally developed female flowers, 3-5 ones were normally developed male flowers, and up to 10-11 ones showed an abnormal way of development. The percentage of abnormal flowers stayed invariable when growing in greenhouse condition with high humidity and temperature as well as in open field condition. As it was shown the development of deformed abnormal flowers inherited and manifested in the following generation after self-pollination. As a result of the study, the occurred anomalies in course of male and female flower development in summer squash (C. pepo L.,) DH-lines produced through a cultivation of unpollinated ovules in vitro were described in details for the first time.
About the Authors
E. A. DomblidesRussian Federation
Ph.D. in Agriculture, Head of Laboratory of Biotechnology
Selectionaya St. 14, VNIISSOK, Odintsovo region, Moscow oblast, 143072, Russia
N. A. Shmykova
Russian Federation
Director of OFR Department, Doctor of Sciences, Professor in Biotechnology
Elisarovykh St. 79/4, Tomsk, 634021, Russia
G. A. Khimich
Russian Federation
Senior Researcher, Laboratory Cucurbits Crop Breeding and Seed Production
Selectionaya St. 14, VNIISSOK, Odintsovo region, Moscow oblast, 143072, Russia
I. B. Korotseva
Russian Federation
Ph.D. in Agriculture, Head of Laboratory Cucurbits Crop Breeding and Seed Production
Selectionaya St. 14, VNIISSOK, Odintsovo region, Moscow oblast, 143072, Russia
A. S. Domblides
Russian Federation
Ph.D. in Agriculture, Head of Laboratory of Genetics and Cytology
Selectionaya St. 14, VNIISSOK, Odintsovo region, Moscow oblast, 143072, Russia
References
1. Kordum E.L., Glushenko G.I. (1976). Cytoembriological aspects and problems of gender in angiosperms. Kiev: Naukova Dumka. P.200.
2. Pangalo K.I. (1943). Gender and flowering in cultivated Cucurbitaceae. Botanical Journal, USSR. V.28. P.10-23.
3. García А., Manzano S., Aguado E., Megнas Z., Martнnez C., Garrido D., Jamilena M. Isolation and Characterization of Three Recessive Andromonoecious Mutants of Cucurbita pepo. Proceedings of Cucurbitaceae 2016, the XIth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae. July 24-28, 2016, Warsaw, Poland. P.45-49.
4. Jeffrey C. (1980). A review of the Cucurbitaceae. Botanical Journal of the Linnean Society, 81(3), 233–247. doi:10.1111/j.1095-8339.1980.tb01676.
5. Manzano S., Megías Z., Martínez C., Jamilena M. (2012) Generation and evaluation of a preliminary EMS mutant library in zucchini squash. In: Sari N., Solmaz I., Aras V. (eds) Cucurbitaceae 2012. Adana: Cukurova University, pp 669–673.
6. Metwally EI., Moustafa S.A., El-Sawy B.I. & Shalaby T.A. (1998) Haploid plantlets derived by another culture of Cucurbita pepo. Plant Cell, Tissue and Organ Culture 52: 171–176, 1998.
7. Murashige T., Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 1962, 15: 473-497 (doi: 10.1111/j.1399- 3054.1962.tb08052.x).
8. Whitaker, T.W. (1931) Sex ratio and sex expression in the cultivated Cucurbits. Amer.J.Bot., 1931, 18, p.359.
9. Wang Y.H., Behera T.K., Kole C., editors. (eds.). (2011). Genetics, Genomics and Breeding of Cucurbits. CRC Press.
Review
For citations:
Domblides E.A., Shmykova N.A., Khimich G.A., Korotseva I.B., Domblides A.S. DEVELOPMENT OF ABNORMAL FLOWERS IN GENERATION OF DOUBLED HAPLOIDS OF SUMMER SQUASH (CUCURBITA PEPO L.). Vegetable crops of Russia. 2018;(5):13-17. (In Russ.) https://doi.org/10.18619/2072-9146-2018-5-13-17