THE INFLUENCE OF METHOD STORAGE ON FUNCTIONAL PARAMETERS OF POLLEN OF TABLE BEET
https://doi.org/10.18619/2072-9146-2018-4-51-54
Abstract
When working with pollen, it is important to keep its functional parameters for some time. To do this, you need to choose the best conditions to preserve pollen germination. The influence of different method of pollen storage of inbred plants of beetroot on its functional characteristics, at germination on artificial nutrient medium under conditions in vitro. When storing pollen in eppendorf tube for the second-third day, the pollen germination decreased by 3-10 times compared to the initial value, after a week of storage, pollen was in conglomerates, a lot of burst pollen. When stored on the sprigs for the seventh to eighth days, the decrease in pollen germination was no more than 30% of the control, and the growth rate of the pollen tube in most samples remained approximately at the control level. For a comparative analysis of the germination of pollen of inbred plants beetroot when evaluating a large set of samples, it is recommended to store flowering twigs in paper bags at a reduced temperature (10-12°C). This is important when working with a large set of samples where it is not possible to compare them at the same time.
About the Authors
E. G. Kozar
FSBSI Federal Scientific Vegetable Center
Russian Federation
Russian Federation
PhD in agriculture, leading researcher of the laboratory of new technologies,
Selectionnaya str., 14, p. VNIISSOK, Odintsovo district, Moscow region, 143072
S. A. Vetrova
FSBSI Federal Scientific Vegetable Center
Russian Federation
Russian Federation
PhD in agriculture, senior researcher of the laboratory of breeding and seed production of table root crops,
Selectionnaya str., 14, p. VNIISSOK, Odintsovo district, Moscow region, 143072
M. I. Fedorova
FSBSI Federal Scientific Vegetable Center
Russian Federation
Russian Federation
PhD in agriculture, professor, Principal Scientist of the laboratory of breeding and seed production of table root crops,
Selectionnaya str., 14, p. VNIISSOK, Odintsovo district, Moscow region, 143072
References
1. Hormaza J.I., Herrero M. Pollen selection // Theor. Appl Genet., 1992. Vol 83. P.663-672.
2. Ottaviano E., Mulcahy D.L. Genetics of angiosperm pollen // Advances in genetics, 1989. Vol.2. P. 61-64.
3. Vetrova S.A. Source material for breeding for heterosis beetroot / Avtoref. Diss.... kand. of agricultural Sciences: 06.01.05. Moscow, 2011. 158 p.
4. Bunin M.S., Shmykova N.A., Bocharnikova N.I., Pyshnaya O.N., Dzhos Y.A. Methodical recommendations on determination of germination pollen of the Capsikum annuum L. Moscow. 2004. P.9.
5. Methodological guidelines for gametes breeding of agricultural plants (methodology, results and perspectives) / ed. Pivovarov V.F. M.2001. 390p.
6. Kozar E.G., Bespalko L.V.; Balashova N.N., Balashova I.T., Pyshnaya O.N., Engalycheva I.A. Influence of storage conditions on germination of pollen of Capsikum annuum L. // Materials of international scientific-practical conference "Innovative technologies in plant breeding and seed growing of agricultural cultures" devoted to 125th anniversary of S.I. Zhegalov. M: VNIISSOK. 2006. T.2. P.139-145.
7. Zhuzhzhalova T.P. Embryology of sugar beet. The development of microgametophyte / Encyclopedia of the genus Beta: biology, genetics, breeding beets /resp. edited by Maletskii S.I. –Novosibirsk. 2010. P.87-93.
8. Pausheva Z.P. Practicum on plant Cytology. Moscow: Agropromizdat, 1988. 271 p.
9. Danvell D.M. Culture of haploid cells // Plant Biotechnology: cell culture / English. edited By R. G. Butenko. Moscow: Agropromizdat, 1989. P.33-51.
10. Dospehov B.A. Methodology of field experience (with the basics of statistical processing of research results). M: Agropromizdat, 1985. 351 p.
11. Kozar E.G., Zayachkovskiy V.A., Fedorova M.I., Balashova I.T., The Influence of temperature on the germination of pollen beetroot in vitro // Science almanac: agricultural science. Tambov. 2015. No.11(4/13). P.322-327.
12. Brewbaker J.L., Kwack B.H. The essential role of calcium ion in pollen germination and pollen tube grоwth //American Journal of Botany. 1963. V.50. P.239-263.
Review
For citations:
Kozar E.G., Vetrova S.A., Fedorova M.I. THE INFLUENCE OF METHOD STORAGE ON FUNCTIONAL PARAMETERS OF POLLEN OF TABLE BEET. Vegetable crops of Russia. 2018;(4):51-54. (In Russ.) https://doi.org/10.18619/2072-9146-2018-4-51-54
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