Searching for sterility genes in bulb onion breeding accessions with the use of DNA markers
https://doi.org/10.18619/2072-9146-2019-5-15-19
Abstract
Relevance. Sterility is a very important trait that is indispensable for hybrid production. Genetic factor underlying in plant sterility can be now identified in large plant populations by DNA markers with high effectiveness and reliability. The evaluation of such markers enables to define their current applicability in breeding program.
Methods. The markers from different publications that had been successfully used were taken to test their effectiveness on 19 accessions of bulb onion (Allium cepa L.).
Results. Mitochondrial genes 5’cob, orf725 and orfA501 and alleles of fertility restoring locus Ms were also identified. Four breeding accessions had S-cytoplasm, nine accessions were with T-cytoplasm and six shared normal cytoplasm not showing any sterility gene in the analysis. As a result of marker testing, the all compositions of the genes in cytoplasm and Ms alleles in nucleus affecting the sterility had been revealed, such as one sterility maintainer, one male sterile accession, and two fertility restorers. However, it should be noted that not all markers tested were in accordance with each other, where the markers originated from chloroplast DNA of did not confirmed the results obtained with those cytoplasm-origins. As it was shown the practical use of the set of markers makes it possible to reveal necessary accessions with required gene composition for hybrid production in bulb onion.
About the Author
Artur S. Domblides
FSBSI Federal Scientific Vegetable Center
Russian Federation
Russian Federation
Ph.D. in Agriculture, Head of Laboratory of Genetics and Cytology
Selectionaya St. 14, VNIISSOK, Odintsovo region, Moscow oblast, 143080
References
1. Hanson M.R. Phylogenetic relationship among cultivated Allium species from restriction enzyme analysis of the chloroplast genome. Theor. Appl. Genet. 1991;81:752-757. DOI: 10.1007/BF00224985
2. Jones, H.A., and Mann, L.K. Onion and their Allies. Leonard Hill Ltd, 1963, London, pp 286.
3. Jones H.A., Clarke A.E. Inheritance of male sterility in the onion and the production of hybrid seed. Proc. Am. Soc. Hort. Sci. 1943;43:189–194.
4. Kaul, M.L.Y. Male sterility in higher plants, Monogr in Theor. Appl. Genet, 1988, Vol. 9, Springer-Verlag, Berlin, pp. 1005. DOI: 10.1007/978-3-642-83139-3
5. Berninger E. Contribution a l’etude de la sterilite de male de l’oignon (Allium cepa L.). Ann Amelior. Plant (Paris) 1965. – V.23. – P. 183-199.
6. Havey M. J. Diversity among male-sterility inducing and male-fertile cytoplasms of onion. Theor. Appl. Genet. 2000;101:778-782. DOI: 10.1007/s001220051543
7. De Courcel A.G.L., Vedel F., Boussac J. M. DNA polymorphism in Allium cepa cytoplasms and its implications concerning the origin of onions. Theor. Appl. Genet. 1989;77:793–798. DOI: 10.1007/BF00268328
8. Havey M.J. A putative donor of S-cytoplasm and its distribution among openpollinated population of onion. Theor. Appl. Genet. 1993;86:128-134. DOI: 10.1007/BF00223817
9. Holford P., Croft J.H., Newbury H.J. Differences between, and possible origins of the cytoplasms found in fertile and male-sterile onions (Allium cepa L.). Theor. Appl. Genet. 1991a;82:737–744. DOI: 10.1007/BF00227319
10. Havey M.J. Identification of cytoplasms using the polymerase chain reaction to aid in the extraction of maintainer lines from open-pollinated populations of onion. Theor. Appl. Genet. 1995;90:263–268. DOI: 10.1007/BF00222212
11. Holford P., Croft J.H., Newbury, H.J. Structural studies of microspogenesis in fertile and male sterile onions (Allium cepa L.) containing the CMS-S cytoplasm. Theor. Appl. Genet. 1996b;82:745-755. DOI: 10.1007/BF00227320
12. Sato Y. PCR amplification of CMS-specific mitochondrial nucleotide sequences to identify cytoplasmic genotypes of onion (Allium cepa L.). Theor. Appl. Genet. 1998;96:367–370. DOI: 10.1007/s00122-003-1230-3
13. Engelke T, Terefe D, Tatlioglu T. A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L.). Theor. Appl. Genet. 2003;107:162–167. DOI: 10.1007/s00122-003-1230-3
14. Kim S., Lee E., Cho D. Y., Han T., Bang H., Patil B. S., Ahn Y. K., and Yoon M. Identification of a novel chimeric gene, orf725, and its use in development of a molecular marker for distinguishing three cytoplasm types in onion (Allium cepa L.). Theor. Appl. Genet. 2009;118:433-441. DOI: 10.1007/s00122-008-0909-x
15. Gökҫe A.F., Havey M.J. Linkage equilibrium among tightly linked RFLPs and the Ms locus in open-pollinated onion populations. J. Am. Soc. Hortic. Sci. 2002;127:944–946. DOI: 10.21273/JASHS.127.6.944
16. Martin W.J., McCallum J., Shigyo M., Jakse J., Kuhl J.C, Yamane N., Pither-Joyce M., Gökçe A.F., Sink K.C., Town C.D., Havey M.J. Genetic mapping of expressed sequences in onion and in silico comparisons with rice show scant colinearity. Mol. Genet. Genomics. 2005;274:197–204. DOI: 10.1007/s00438-005-0007
17. Bang H., Cho D.Y., Yoo K.S, Yoon M.K., Patil B.S., Kim S. Development of simple PCR-based markers linked to the Ms locus, a restorer-of-fertility gene in onion (Allium cepa L.). Euphytica 2011a;179:439–449. DOI: 10.1007/s10681-010-0342-5
18. Bang H., Kim S., Park S.O., Yoo K.S., Patil B.S. Development of a codominant CAPS marker linked to the Ms locus controlling fertility restoration in onion (Allium cepa L.). Sci.Hortic. 2013;153:42–49. DOI: 10.1016/j.scienta.2013.01.020
19. Park J., Bang H., Cho D., Yoon M-K., Patil B., Kim S. Construction of high-resolution linkage map of the Ms locus, a restorer-of-fertility gene in onion (Allium cepa L.). Euphytica. 2013;192:267–278. DOI: 10.1007/s10681-012-0851-5
20. Huo Y.M., Miao J., Liu B.J., Yang Y.Y., Zhang Y.H., Wu X. The expression of pectin methylesterase in onion flower buds is associated with the dominant male-fertility restoration allele. Plant. Breed. 2012;131:211–216. DOI: 10.1111/j.1439-0523.2011.01907.x
21. Yang Y.Y., Huo Y.M., Miao J., Liu B.J., Kong S.P., Gao L.M., Liu C., Wang Z.B., Tahara Y., Kitano H., Wu X. Identification of two SCAR markers co-segregated with the dominant Ms and recessive ms alleles in onion (Allium cepa L.). Euphytica. 2013;190:267–277. DOI: 10.1007/s10681-012-0842-6.
22. Kim S. A codominant molecular marker in linkage disequilibrium with a restorer-of-fertility gene (Ms) and its application in reevaluation of inheritance of fertility restoration in onions. Mol. Breed. 2014;34:769–778. DOI: 10.1007/s11032-014-0073-8
23. Huo, Y.M., Liu, B.J., Yang, Y.Y., Miao J., Gao L.M., Kong S.P., Wang Z.B., Kitano H., Wu X. AcSKP1, a multiplex PCR-based co-dominant marker in complete linkage disequilibrium with the male-fertility restoration (Ms) locus, and its application in open-pollinated populations of onion. Euphytica. 2015;204:711. DOI: 10.1007/s10681-015-1374-7
24. Suprunova T.P., Logunov A.N., Logunova V.V., Agafonov A.F. Determination of cytoplasmic male sterile factors in onion plants (Allium cepa L.) of VNIISSOK's breeding. Vegetable crops of Russia, 2011;(4):20-21. (In Russ.)]
25. Pavlova I.V., Kupreenko N.P., Bulahova A.S. DNA markers in onion (Allium cepa L.) cytoplasmic male sterility study. Vegetable crops of Russia. 2018;(4):16-19. (In Russ.) DOI: 10.18619/2072-9146-2018-4-16-19
26. Kim, B., Yang, TJ. & Kim, S. Identification of a gene responsible for cytoplasmic male-sterility in onions (Allium cepa L.) using comparative analysis of mitochondrial genome sequences of two recently diverged cytoplasms. Theor. Appl. Genet. 2019;132:313. DOI: 10.1007/s00122-018-3218-z
27. Von Kohn C., Kiełkowska A., Havey M.J. Sequencing and annotation of the chloroplast DNAs and identification of polymorphisms distinguishing normal male-fertile and male-sterile cytoplasms of onion. Genome. 2013;56:737–742. DOI: 10.1139/gen-2013-0182
28. Khar A., Saini N. Limitations of PCR-based molecular markers to identify male-sterile and maintainer plants from Indian onion (Allium cepa L.) populations. Plant. Breed. 2016;135(4):519-524. DOI: 10.1111/pbr.12373
Review
For citations:
Domblides A.S. Searching for sterility genes in bulb onion breeding accessions with the use of DNA markers. Vegetable crops of Russia. 2019;(5):15-19. (In Russ.) https://doi.org/10.18619/2072-9146-2019-5-15-19
Views:
743
Email this article 