An integrated approach to the analysis of promising potato breeding material in the conditions of the Omsk region

Relevance. Integrated methods of field analysis and PCR diagnostics of potato varieties allow us to evaluate genotypes suitable for breeding in the Western Siberia zone. The paper presents the results of the study carried out at the Omsk Agrarian Scientific Center in 2021- 2023. The purpose of the study is to conduct an integrated assessment using laboratory and field diagnostics to identify potato varieties with a set of economically useful traits.

Material and Methods. A collection of 23 potato samples was studied in the experiments. The cultivation technology is adopted for the zone. The predecessor is spring wheat. Observations and records were carried out in accordance with the "Methodological recommendations of the All-Russian Research Institute of Potato Growing", "Guidelines for specialized assessment of potato varieties" and the "Modern methods of pathogen diagnostics.". Statistical data processing was carried out according to the method of B.A. Dospekhov. Determination of starch content was carried out by specific gravity in combination with molecular diagnostics, including a primer for determining the starch content gene, and a polymerase chain reaction was also carried out using SSR markers that determine resistance to viruses X, Y, S, L and late blight.

Results. As a result of the combination of approaches, samples with a complex of features were isolated, namely, Khozayushka – yield – 24.3 t / ha, a complex of genes for resistance to viruses X / L / S, starch content – 18.6%, no wateriness, flouriness and high taste. Vecherniy Omsk – yield – 24.7 t/ha, presence of genes for resistance to late blight. Spectr – yield – 24.4 t/ha, complex of genes for resistance to X/L/S viruses, starch content – 17.3%. Sample 52-17 – yield – 23.6 t/ha, presence of genes for resistance to late blight, complex of genes for resistance to Y/L/S viruses. Gala – yield – 25.3 t/ha, presence of genes for resistance to late blight, no darkening of cooked pulp.

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