Studying the morphological structures of starch granules in potato cells (Solanum tuberosum L.) using the method of confocal laser scanning microscopy
https://doi.org/10.18619/2072-9146-2025-1-102-107
Abstract
Relevance. Potato are one of the main sources of starch. Potato starch is widely used in human economic activities. The starch content in tubers, including the number of starch granules and their size and shape, are varietal characteristics. For this reason, it is important to identify potato varieties with starch grains suitable for starch production already at the early stages of breeding process.
Materials and Methods. The Aim was to study the morphological structure of starch granules in cells of potato tubers belonging to varieties from different maturity groups. Eighteen potato varieties (Solanum tuberosum L.) of various breeding origin and from different maturity groups were used as the research object. The granulometric composition was determined by the method of fluorescent microscopy. The research was conducted using a CLSM 800 confocal laser scanning microscope (Zeiss, Germany) with a laser wavelength of 488 nm.
Results. The research allowed us to obtain data on the morphology of variously-shaped starch granules in cells: round, oval, elongated, and irregular. The research established a direct correlation between the number of large starch grains in a cell and the time required for a given potato genotype to mature. Mid-season and medium late potato varieties were observed to have a higher starch content than varieties from the groups with earlier maturity. On average, the difference in the starch content between these groups was 1.97%. The highest number of starch grains was found in 40-70 μm cells and larger. The research identified Solanum tuberosum L. genotypes with large and small starch granules. These genotypes might be recommended for further use in the practical breeding to create high-quality varieties.
About the Authors
I. V. Kim
Federal Scientific Center of Agricultural Biotechnology of the Far East named after A.K. Chaiki
Russian Federation
Russian Federation
Irina V. Kim – Chief Researcher, Laboratory for Diagnostics of Potato Diseases
30, Volozhenina st., Timiryazevsky stl., Ussuriysk, Primorsky kray, 692539
D. I. Volkov
Federal Scientific Center of Agricultural Biotechnology of the Far East named after A.K. Chaiki
Russian Federation
Russian Federation
Dmitry I. Volkov – Head of the Potato and Vegetable Growing Department
30, Volozhenina st., Timiryazevsky stl., Ussuriysk, Primorsky kray, 692539
A. G. Klykov
Federal Scientific Center of Agricultural Biotechnology of the Far East named after A.K. Chaiki
Russian Federation
Russian Federation
Aleksei G. Klykov – Head of Department, Department of Selection and Biotechnology Crops
30, Volozhenina st., Timiryazevsky stl., Ussuriysk, Primorsky kray, 692539
References
1. Tetlow I.J. Starch Biosynthesis in Crop Plants. Agronomy. 2018;(8):81. https://doi.org/10.3390/agronomy8060081
2. Bertoft E. Understanding Starch Structure: Recent Progress. Agronomy. 2017;7(3):56. https://doi.org/10.3390/agronomy7030056
3. Ruskina A.A., Popova N.V., Naumenko N.V. et al. Analysis of Contemporary Methods of Modification of Starch as an Instrument of Enhancing its Technological Properties. Bulletin of the South Ural State University. Ser. Food and Biotechnology. 2017;5(3):12–20. https://doi.org/10.14529/food170302
4. Lizarazo H.S.P., Hurtado R.G.G., Rodríguez C.L.F. Physicochemical and morphological characterization of potato starch (Solanum tuberosum L.) as raw material for the purpose of obtaining bioethanol. Agronomía Colombiana. 2015;33(2):244-252. https://doi.org/10.15446/agron.colomb.v33n2.47239
5. Shirani-Bidabadi M., Nazarian-Firouzabadi F., Sorkheh K. et al. Transcriptomic analysis of potato (Solanum tuberosum L.) tuber development reveals new insights into starch biosynthesis. PLoS One. 2024;19(4). https://doi.org/10.1371/journal.pone.0297334
6. Ulbrich M., Salazar M. L., Flöter E. Separation and molecular characterization of the amylose- and amylopectin-fraction from native and partially hydrolyzed potato starch. Starch - Stärke. Vol. 69. Issue 7-8. https://doi.org/10.1002/star.201600228
7. Litvyak V.V., Zabolotets A.A., Simakov E.A., Mitushkin A.V., Zhuravlev A.A., Kostenko V.G.Features of the morphological structure of starch granules in various potato varieties. Achievements of science and technology in agro-industrial complex. 2019;33(11):55-59. (In Russ.) https://doi.org/10.24411/0235-2451-2019-11112 https://www.elibrary.ru/thpmqh
8. Brust H., Orzechowski S., Fettke J. Starch and Glycogen Analyses: Methods and Techniques // Biomolecules. 2020;10(7):1020. https://doi.org/10.3390/biom10071020
9. Khlestkin V.K., Peltek S.E., Kolchanov N.A. Target genes for development of potato (Solanum tuberosum L.) cultivars with desired starch properties (review). Agricultural biology. 2017;52(1):25-36. (In Russ.) https://doi.org/10.15389/agrobiology.2017.1.25rus https://www.elibrary.ru/yfqfch
10. Andersson M., Turesson H., Nicolia A. et al. Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts. Plant Cell Rep. 2017;(36):117-128. https://doi.org/10.1007/s00299-016-2062-3
11. Hou J., Liu T., S. Reid et al. Silencing of α-amylase StAmy23 in potato tuber leads to delayed sprouting. Plant Physiology and Biochemistry. 2019;(139):411-418. https://doi.org/10.1016/j.plaphy.2019.03.044
12. Schonhals E.M., Ortega F., Barandalla L. et al. Identification and reproducibility of diagnostic DNA markers for tuber starch and yield optimization in a novel association mapping population of potato (Solanum tuberosum L.). Theor. Appl. Genet. 2016;(129):767-785. https://doi.org/10.1007/s00122-016-2665-7
13. Khlestkin V.K., Erst T.V., Rozanova I.V. et al. Genetic loci determining potato starch yield and granule morphology revealed by genome-wide association study (GWAS). PeerJ. 2020;(8):e10286. doi: 10.7717/peerj.10286
14. Khlestkin V.K., Rozanova I.V., Efimov, V.M. et al. Starch phosphorylation associated SNPs found by genome-wide association studies in the potato (Solanum tuberosum L.). BMC Genet. 2019;(20):29. https://doi.org/10.1186/s12863-019-0729-9
15. Larder C., Baeghbali V., Pilon C. et al. Effect of Non-Conventional Drying Methods on In Vitro Starch Digestibility Assessment of Cooked Potato Genotypes. Foods. 2019;8(9):1-14. https://doi.org/10.3390/foods8090382
16. Zhao F., Jing L., Wang D. et al. Grain and starch granule morphology in superior and inferior kernels of maize in response to nitrogen. Sci Rep. 2018;(8):6343. https://doi.org/10.1038/s41598-018-23977-0
17. Litvyak V.. Size and morphological features of native starch granules of different botanical origin. Ukrainian Food Journal. 2018;7(4):563-576. https://doi.org/10.24263/2304-974X-2018-7-4-3
18. Ilchuk R.V. Productivity and starchiness of potato tubers depending on the maturity group of the variety and weather and climatic conditions during the growing season. Bulletin of the Belarusian State Agricultural Academy. 2014;(2):81-84. (In Russ.) https://www.elibrary.ru/zcrjjh
19. Zhang X., Cheng Y., Jia X. et al. Effects of Extraction Methods on Physicochemical and Structural Properties of Common Vetch Starch. Foods. 2022;(11):2920. doi.org/10.3390/foods11182920
20. Li K., Zhang T., Zhao W. et al. Characterization of starch extracted from seeds of Cycas revolute. Front Nutr. 2023;(10):1159554. https://doi.org/10.3389/fnut.2023.1159554
Review
For citations:
Kim I.V., Volkov D.I., Klykov A.G. Studying the morphological structures of starch granules in potato cells (Solanum tuberosum L.) using the method of confocal laser scanning microscopy. Vegetable crops of Russia. 2025;(1):102-107. (In Russ.) https://doi.org/10.18619/2072-9146-2025-1-102-107
Views:
192
Email this article 