Effect of alanine on accumulation of phenolic compounds in the leaves of chicory (Cichorium intybus L.)
https://doi.org/10.18619/2072-9146-2023-5-62-67
Abstract
Common chicory (Cichorium intybus L.) is a promising source of raw materials for both food (roots) and pharmaceutical industries (leaves). One of the ways to increase the biologically active compounds content in medicinal plant materials is the use of various growth stimulants, which include amino acids. The article presents the results of an experimental study conducted in 2021-2022 which study the effect of of L-alanine and a racemic mixture of alanine (DL) on the accumulation and qualitative composition of phenolic compounds (PC) in the leaves of chicory.
Results. It was found that the use of alanine in different concentrations did not have a significant effect on the increase of phenolic compounds content, and also did not affect the on their accumulation dynamics. The use of alanine enantiomers did not affect the qualitative composition of phenolic compounds, but led to a change in the ratio of hydroxycinnamic acids to each other. It has been established that during the growing season the percentage ratio between the main phenolic compounds in chicory leaves changes - the content of chlorogenic acid increases in proportion to the decrease in chicory acid. Treatment with L-alanine at a concentration of 25 mg/l during the study period in both varieties led to the largest reduction in the content of chicory acid compared to others. It was noted that the total content of PC is higher in a year with warmer and more arid weather, it was higher on average by 0,7 %.
About the Authors
P. O. Mavrina
All-Russian scientific research Institute of medicinal and aromatic plants
Russian Federation
Russian Federation
Polina O. Mavrina – Junior Researcher Department of Chemistry of Natural Compounds
7, Grina street, Moscow,117216
G. V. Adamov
All-Russian scientific research Institute of medicinal and aromatic plants
Russian Federation
Russian Federation
Grigoriy V. Adamov – Leading Researcher laboratories of atomic and molecular bioregulation and selection
7, Grina street, Moscow,117216
E. L. Malankina
All-Russian scientific research Institute of medicinal and aromatic plants
Russian Federation
Russian Federation
Elena L. Malankina – Doc. Sci. (Agriculture), Professor, Chief Researcher Laboratories Botanical Garden
7, Grina street, Moscow,117216
References
1. Abdelhamid M.T., Sadak M., Schmidhalter U. Effect of foliar application of aminoacids on plant yield and physiological parameters in bean plants irrigated with seawater. Acta Biológica Colombiana, 2015;20(1):140-152. DOI: 10.15446/abc.v20n1.42865.
2. Samad A., Shaukat K., Ansari M., Nizar M., Zahra N. et al. Role of foliar spray of plant growth regulators in improving photosynthetic pigments and metabolites in Plantago ovata (Psyllium) under salt stress – A field appraisa. BIOCELL. 2023;47(3):523–532. DOI: 10.32604/biocell.2023.023704.
3. Petukhov D.V., Izmest’ev E.S., Sazanov A.V., Zaitsev M.A., Tovstik E.V. The use of amino acids and their chelate complexes with trace elements in plant nutrition (review). Theoretical and Applied Ecology. 2022;(1):167- 174. DOI: 10.25750/1995-4301-2022-1-167-174. EDN TGQXRF. (In Russ.)
4. Haydon M.J., Cobbett C.S. Transporters of ligands for essential metal ions in plants. The New Phytologist. 2007;174(3):499-506. DOI: 10.1111/j.1469-8137.2007.02051.x.
5. Malankina E.L., Terechova V.I., Zujkova E.Yu. [Peppermint propagation technology development for organic culture]. Bulliten KSAU. 2022;3(180):10-16. DOI: 10.36718/1819-4036-2022-3-10-16. EDN SZQRVJ. (In Russ.)
6. Khalesi A., Mousavi Mirkalaeia S.A., Modarres Sanavy S.A.M. et al. Effect of foliar application of amino acids on grain yield and physiological traits of chickpea under drought stress. Gesunde Pflanzen. 2023. DOI: 10.1007/s10343-022-00821-0.
7. Berry Z.C., Emery N.C., Gotsch S.G., Goldsmith G.R. Foliar water uptake: Processes, pathways, and integration into plant water budgets. Plant. Cell & Environment. 2019;42(2):410–423. DOI: 10.1111/pce.13439.
8. Malankina E., Potschuev P., Malankin G., Zaitchik B., Ruzhitskiy A. Amino Acids for Medicinal Plants of the Umbellifer Family (Apiaceae). Zeitschrift fur Arznei - und Gewurzpflanzen. 2022.
9. Mavrina P.O., Saybel O.L., Malankina E.L. Possibilities of using leaves cultivated chicory (Cichorium intybus L.) as a medicinal plant material (review). Vegetable crops of Russia. 2021;(4):105-110. (In Russ.) https://doi.org/10.18619/2072-9146-2021-4-105-110. EDN AQTNMH.
10. Saybel O.L., Radimich A.I., Dargaeva T.D., Mavrina P.O. [Secondary raw materials of chicory and topinambur - a source of phenolic compounds]. Phenolic compounds: fundamental and applied aspects. 2022. (In Russ.).
11. Saibel O.L., Dargaeva T.D., Tsitsilin A.N., Dul V.N. [Development of a method of quantitative analysis of biologically active substances and assessment of accumulation dynamics depending on vegetation phase of common chicory (Cichorium intybus L.)]. Problems of biological, medical and pharmaceutical chemistry. 2016;19(6):20-24. URL: https://bmpcjournal.ru/sites/default/files/private/bmfc-2016-06-05.pdf (In Russ.)
12. Saybel O.L., Radimich A.I., Adamov G.V., Dargaeva T.D., Fadeev N.B., Zelenkov V.N., Lapin A.A. The chemical composition of the fractions of the aerial part of cultivated chicory and their anti-oxidant activity. Khimiya Rastitel'nogo Syr'ya. 2021;(4):165-173. DOI: 10.14258/jcprm.2021049316. EDN JZOTGD. (in Russ.)
13. Tsai K.L., Kao C.L., Hung C.H., Cheng Y.H., Lin H.C., Chu P.M. Chicoric acid is a potent anti-atherosclerotic ingredient by anti-oxidant action and anti-inflammation capacity. Oncotarget, 2017;8(18):29600–29612. DOI: 10.18632/oncotarget.16768.
14. Adem Ş., Eyupoglu V., Sarfraz I., Rasul A., Zahoor A.F., Ali M., Abdalla M., Ibrahim I.M., Elfiky A.A. Caffeic acid derivatives (CAFDs) as inhibitors of SARS-CoV-2: CAFDs-based functional foods as a potential alternative approach to combat COVID-19. Phytomedicine. 2020. DOI: 10.1016/j.phymed.2020.153310.
15. Saybel O.L., Dargaeva T.D., Pupykina K.A. The study of choleretic and hepatoprotective activity of Cichórium íntybus L. Medical Bulletin of Bashkortostan. 2015;10(5):70-73. EDN VRFLLJ. (In Russ.)
16. Saybel O.L., Dargaeva T.D., Pupykina K.A., Petrova I.V., Farkhutdinov R.R. Assessment of antioxidant activity of witloof chicory herb (Cichorium intybus L.). Acta Biomedica Scientifica (East Siberian Biomedical Journal). 2017; Т.2,2(114):85-88. DOI 10.12737/article_59a614fcd18c42.95236968. EDN YMRKIN. (In Russ.)
17. Yang M., Wu C., Zhang T., Shi L., Li J., Liang H., Lv X., Jing F., Qin L., Zhao T.,Wang C., Liu G., Feng S., Li F. Chicoric Acid: Natural Occurrence, Chemical Synthesis, Biosynthesis, and Their Bioactive Effects. Frontiers in Chemistry. 2022. DOI: 10.3389/fchem.2022.888673
Review
For citations:
Mavrina P.O., Adamov G.V., Malankina E.L. Effect of alanine on accumulation of phenolic compounds in the leaves of chicory (Cichorium intybus L.). Vegetable crops of Russia. 2023;(5):62-67. (In Russ.) https://doi.org/10.18619/2072-9146-2023-5-62-67
Views:
337
Email this article 