Cultivation of Brassica rapa L. subsp. nipposinica plants at the multi circle hydroponic installation with help of natural immunomodulators

Relevance. Modern vegetable production in greenhouses is based on high tech-advantageous, such as vertical hydroponic technology. Increasing of vertical hydroponic market is conditioned with ensuring the food security of states in conditions instability of food deliveries due to violations of traditional logistic connections. The program of vertical tomato hydroponics was developed and used in practice at the FSBSI FSVC in 2010-2020. Productivity potential of Brassica rapa L. subsp. nipposinica cultivated at vertical hydroponic installation have been studied in connection with the spread of consumption of fresh greens and “yellow-green” vegetables.

The goal of the study was: the productivity characteristics and analysis of biochemical composition Brassica rapa L. subsp. nipposinica cultivated at the five-circle vertical hydroponic installation with help of natural immunomodulators.

Materials and methods. Materials of study: seeds and leaves (rosette of leaves) from 2 varieties of Brassica rapa L. subsp. nipposinica – Japanese variety Salad Mizuna and new variety Salut Jubileiu (originated from FSBSI FSVC); immune modulators – flavonoid glycoside linarozide and steroid glycosides moldstim and trigonellozide.

Methods. Seed treatment with 0,005%-water solutions of immune modulators; cultivation plants Brassica rapa L. subsp. nipposinica at the five-circle vertical hydroponic construction; analytical methods: determination of dry meter; determination the sum of antioxidants in leaves of Brassica rapa L. subsp. nipposinica; biometry; statistical methods: one-factorial dispersion analysis and two-factorial dispersion analysis.

Results. Possibility of Brassica rapa L. subsp. nipposinica plants cultivation at multi circle hydroponic installations was established by authors. Seed treatment with water solutions of immune modulators wasn’t influence on seedlings appearing (the quality of seeds was very high -99-100%). Height of plant (of rosette) the variety Mizuna was increased as the reaction on the seed treatment, but the weight of plants wasn’t change. Salut Jubileiu variety reacted with increasing the common weight of plants. Number of leaves was increased. The content of dry matter in leaves didn’t change, but the sum content of antioxidants is rise significantly after seed treatment of immune modulators. The conclusion has been made: applying of immune modulators from glycosides class could to increase the productivity, vitamin value and safety cultivation of Brassica rapa L. subsp. nipposinica plants at multi circle hydroponics installation. 

1. Global Industry Report, 2014-2025. April, 2017. Report ID: IVR 1-68038-797-1.

2. URL:https://toeplitz.ru/hydro/prognoz-razvitija-rynka-gidroponiki.html (дата обращения 17.11.2021)

3. Balashova I., Sirota S., Pinchuk Ye. Vertical vegetable growing: creating tomato varieties for multi-tiered hydroponic installations. International Conference on Sustainable Development for Cross-Border Regions. IOP Conference Series: Earth and Environmental Science. 2019;395(2019)012079:1-8. https://doi.org/10.1088/1755-1315-395-1-012079

4. Balashova I.T., Sirota S.M., Pinchuk E.V., Vershinina N.P .New energy saved technology of Federal State Budgetary Scientific Institution “Federal Scientific Vegetable Center”. Agrarian Science for Agriculture. Proceedings of XVII International Scientific-Practical Conference. Barnaul. 2022. Р.180-182. (In Russ.)

5. Artemieva A.M., Soloviova A.E. Genetic diversity and biochemical value of cabbage vegetable plants of the genus Brassica L. Bulletin of Novosibirsk State Agrarian University. 2018;(4):50-61. (In Russ.)

6. Pivovarov V.F., Tareeva M.M., Mukhortov V.Yu., Golubkina N.A., Zamana S.P., Kosheleva O.V. Japanese cabbage is high-value vegetable culture. Potato and vegetables. 2009;(9):13. (In Russ.)

7. Artemieva A.M. New incoming accessions of Brassica rapa L. into the VIR plant collection. Vegetable crops of Russia. 2017;(2):14-19. (In Russ.) https://doi.org/10.18619/2072-9146-2017-2-14-19

8. Bondareva L.L., Mineykina A.I., Paslova T.O., Molchanova A.V., Paslova N.O. Japanese cabbage: features of morphological and biochemical parameters of a promising sample. Vegetable crops of Russia. 2020;(6):62-66. (In Russ.) https://doi.org/10.18619/2072-9146-2020-6-62-66

9. Balashova N.N., Zhuchenko A.A., Pivovarov V.F., Balashova I.T., Kozari E.G., Bespal’ko A.V., Pishnaya O.N., Kintya P.K., Lupashku G.A., Maschenko N.E., Shvets S.A., Bobeikă V.A. Regulation of Phytopathosystem Stability With Help of Plant Secondary Metabolites. Agricultural Biology. 2004;(1):3- 16. (In Russ.)

10. Kintya P.K., Lazurievskyi G.V., Balashova N.N., Balashova I.T., Surudgiu A.I., Liakh V.A. Structure and biological activity steroid glycosides of furostane and spirostane- rows. Chişinău:ştiinţa. 1987. 141 p. (In Russ.)

11. Lutsenko A.K., Doroshenko I.V. Influence of biologically active compounds on physiological characteristics of cucumber plants and their sensitivity to CMV. Inductive resistance of agricultural crops to pathogens. Rostovon-Don. 1989. Р.25-27. (In Russ.)

12. Maschenko N.E., Borovskaya A.D., Gumaniuk A.V., Balashova I.T., Cozar E.G. Efficiency of natural growth regulators in carrot production. Vegetable crops of Russia. 2018;(1):74-78. (In Russ.) https://doi.org/10.18619/2072-9146-2018-1-74-78

13. Dospekhov B.A. Methods for field experiments. Moscow: Agropromizdat, 1985. 351 p. (In Russ.)

14. Sirota S.M., Balashova I.T., Kozar E.G., Mitrofanova O.A., Autko A.A., Dolbik M.A. First Results of Breeding Tomato Varieties and Hybrids For Multicircle Hydroponic. Greenhouses of Russia.2014;(3):58-62. (In Russ.)

15. Kidin V.V. Workshop on Agricultural Chemistry. Moscow:Kolos. 2008. 215 p. (In Russ.)

16. Golubkina N.A., Kekina E.G., Molchanova A.V., Antoshkina M.S., Nadezhkin S.M., Soldatenko A.V. Plant antioxidants and methods for their determination. Moscow: FSBSI FSVC; 2018. 66 p. (In Russ.)

17. Fedotov S.V. Essential oils and their influence on the higher nervous activity of a man.Proceedings of the Great Nikitskyi Botanical Garden. Jalta, Crimea. 2015;(141):131-147. (In Russ.)

18. Novak J. Optimizing plant secondary metabolite production. Plant Breeding: the Art of Brining Science to Life. Abstracts of the 20th EUCARPIA General Congress. Zurich, Switzerland.2016:77. ISBN 978-3-906804-22-4.

19. Koutouan C., Yovanopoulos C., Piquet M., Hugueney P., Halter D., Claudel P., Baltenweck R., Hamama L., Suel A., Huet S., Peltier D., Briard M., Le Clerc V. Can the variation of secondary metabolite contents be part of carrot resistance to Alternaria dauci? Plant Breeding: the Art of Brining Science to Life. Abstracts of the 20th EUCARPIA General Congress. Zurich, Switzerland.2016. С.71. ISBN 978-3-906804-22-4.

20. Balashova I.T., Verderevskaya T.D., Kintya P.K. Antiviral activity of steroid glycosides at the TMV-model. Agricultural biology.1984;(4):64-68. (In Russ.)

21. Zhuchenko A.A., Kintya P.K., Balashova N.N. Method of tomato resistance evaluation to PhytophthorainfestanceDB. Author patent №895365. USSR Bulletin of inventions and discoveries. 1982, №1. (In Russ.)

22. Lahmatova I.T., Kintia P.K., Verderevskaya T.D., Juravel A.M., Ostapenko A.B. One Biochemical Marker of Plum Resistance to PPV. Proceedings of the Middle European Meeting’96 on Plum Pox.Budapest.1997. Р.33-36.

23. Kozar E.G., Engalycheva I.A., Antoshkin A.A., Mashcenco N.E. Screening of biological activity of phytopreparations based on secondary metabolites of plants on the culture of Phaseolus vulgaris. Vegetable crops of Russia. 2021;(5):89-97. (In Russ.) https://doi.org/10.18619/2072-9146-2021-5-89-97

24. Lupashku G.A. Immunological bases of triticale resistance to Fusarium graminearum Link. Doctor habilitate in plant biology thesis. Chişinău. 1999. Р.249-266. (In Russ.)

25. Balashova I.T. Using of Steroid Glycosides (Saponins) in Agriculture. New Trends in Saponins. Book of Abstracts International Conference on Saponins. France. Nancy Université. 2009. Р.14.

26. Balashova I.T., Balashova N.N., Kintia P.K. Steroidal Glycosides as Plant Resistance Inductors. Acta Agronomica Hungarica. 1990;39(1-2):183-191.