Biotechnology methods in virus-free potato seed production
https://doi.org/10.18619/2072-9146-2022-5-29-34
Abstract
Relevance. Plants of potato varieties are carriers of viral pathogens in a latent form. These viruses can be transmitted to clonal progeny of the carriers. The system of virus-free seed production facilitates the elimination of the viruses in seed potatoes and preserves the high productivity of potato varieties. The research goal was to develop a scheme for virus elimination in potato using biotechnological methods and to introduce this scheme in the production of virus-free tubers under the conditions of Primorsky krai.
Material and methods. New promising variety Moryak (breeding number Pri-08-11-1), which was created in FSBSI “FSC of Agricultural Biotechnology of the Far East named after A.K. Chaiki”, was used as the research object. The mean yield of the new genotype is 34.1 t/ha, the potential yield is 40.1 t/ha. The dry matter content is 18.13-23.85%, the starch content is 12.10-17.24%, and the content of vitamin C is 17.46-23.12 mg/100 g. This variety has a high keeping quality of tubers (92.2-94.4%) and resistance to excessive soil moisture. Tissue culture and chemotherapy in combination with ribavirin (a concentration of 0.02-0.03%) and chitosan (0.01-0.1%) were used for virus elimination. Sprouts from the original tubers and plantlets were tested by EIA and qPCR for latent infection (PVX, PVY, PVA, PVS, PVM, PLRV).
Results. A sequential increase in the concentration of ribavirin (from 0.02 to 0.03%) and chitosan (from 0.01 to 0.1%) and their alternation in different passages proved to be an effective method for virus elimination in plantlets. As the result of the research, the new scheme for the elimination of the most economically important potato viruses was developed and introduced, and virus-free seed material was obtained.
About the Authors
I. V. Kim
FSBSI “Federal Scientific Center of Agricultural Biotechnology of the Far East A.K. Chaiki”
Russian Federation
Russian Federation
Irina V. Kim – Cand. Sci. (Agriculture), Leading Researcher
30 B, Volozhenina st., Timiryazevsky stl., Ussuriysk, Primorsky krai, 692539
E. V. Shishchenko
FSBSI “Federal Scientific Center of Agricultural Biotechnology of the Far East A.K. Chaiki”
Russian Federation
Russian Federation
Elena V. Shischenko – Junior Researcher at the Laboratory of Agricultural Biotechnology
30 B, Volozhenina st., Timiryazevsky stl., Ussuriysk, Primorsky krai, 692539
P. V. Fisenko
FSBSI “Federal Scientific Center of Agricultural Biotechnology of the Far East A.K. Chaiki”
Russian Federation
Russian Federation
Petr V. Fisenko – Cand. Sci. (Biology), Leading Researcher, Acting Head of the Laboratory of Breeding and Genetic Research of Field Crops
30 B, Volozhenina st., Timiryazevsky stl., Ussuriysk, Primorsky krai, 692539
A. S. Chibizova
FSBSI “Federal Scientific Center of Agricultural Biotechnology of the Far East A.K. Chaiki”
Russian Federation
Russian Federation
Alena S. Chibizova – Junior Researcher at the Laboratory of Agricultural Biotechnology
30 B, Volozhenina st., Timiryazevsky stl., Ussuriysk, Primorsky krai, 692539
A. G. Klykov
FSBSI “Federal Scientific Center of Agricultural Biotechnology of the Far East A.K. Chaiki”
Russian Federation
Russian Federation
Alexey G. Klykov – Head of the Department of Breeding and Biotechnology of Agricultural Crops, Doctor Sci. (Biology), Academician of the Russian Academy of Sciences
30 B, Volozhenina st., Timiryazevsky stl., Ussuriysk, Primorsky krai, 692539
References
1. Ryabtseva T.V., Kulikova V.I., Khilkevich O.G. Potato health improvement by chemotherapy in in vitro culture. International Scientific Research Journal. 2015;10(41)3:66-68. (In Russ.) https://doi.org/10.18454/IRJ.2015.41.127
2. Simakov E.A., Anisimov B.V., Zhevora S.V., Mityushkin A.V., Zhuravlev A.A., Mityushkin A.V., Gaizatulin A.S. Current trends in the development of potato breeding and seed production in Russia. Potatoes and vegetables. 2020;(12):22-26. (In Russ.) https://doi.org/10.25630/PAV.2020.49.70.005
3. Kim I.V., Klykov A.G. Results and directions of research on potato growing in the Russian Far East. Achievements of science and technology of the agroindustrial complex. 2017;(10):36-39. (In Russ.)
4. Gnutova R.V. Diversity of plant viruses in the East Asian region of Russia: results of 50 years of study. Agricultural biology. 2014;(5):16-27. (In Russ.) https://doi.org/10.15389/agrobiology.2014.5.16rus
5. Volkov Y.G., Kakareka N.N., Kozlovskaya Z.N., Balabanova L.A., Sapotskij M.V. Characterization of a Novel Far Eastern Potato Virus Y. Isolates. Plant Pathology Journal. 2009;8(2):62-67.
6. Pleshakova T.I. Monitoring of potato viral infections in the south of Primorsky Krai / Proceedings of the II International Scientific Conference "Modern research in Natural Sciences", Vladivostok, August 26-28, 2015 [Electronic resource] / edited by V.A. Semal ; Electron. dan. Vladivostok: Publishing House of the Far East. 2015. pp. 186-189. ISBN 978-5-906739-79-7. (In Russ.)
7. Oves E.V., Gabitova N.A. New elements of technology for improving and obtaining basic clones of promising potato varieties and hybrids. Achievements of science and technology of the agro-industrial complex. 2016;30(11):60-62. (In Russ.)
8. Zhevora S.V., Anisimov B.V., Simakov E.A., Oves E.V., Zebrin S.N. Potatoes: problems and prospects. Potatoes and vegetables. 2019;(7):2-7. (In Russ.)
9. Oves E.V., Gaitova N.A., Shishkina O.A., Fenina N.A.Results of selection of basic potato clones in the conditions of the European North and the highlands of the North Caucasus. Agriculture. 2020;(4):29-32. (In Russ.)
10. Yang L., Nie B., Liu J., Song B. A Reexamination of the effectiveness of ribavirin on eradication of viruses in potato plantlets in vitro using ELISA and quantitative RTPCR. Am. J. Potato Res. 8 p. (published online 15 November 2013).
11. Gong H., Igiraneza C., Dusengemungu L. Major In Vitro Techniques for Potato Virus Elimination and Post Eradication Detection Methods. A Review. Am. J. Potato Res. 2019;(96):379–389. https://doi.org/10.1007/s12230-019-09720-
12. Yalovik, A.V., Fedorova Yu. N., Yalovik L. I. The effectiveness of chemotherapy on potato plants under in vitro conditions. Bulletin of the Altai State Agrarian University. 2019;10(180):28-33. EDN SWVPLP. (In Russ.)
13. Bespalova E.S., Agakhanov M.M., Arkhimandritova S.B., Erastenkova M.V., Uhatova Yu.V. Sanitization of potato varieties from the VIR collection against viruses. Proceedings on applied botany, genetics and breeding. 2020;181(4):164-172. (In Russ.) https://doi.org/10.30901/2227-8834-2020-4-164-172
14. Cassells A.C., Long R.D. The elimination of potato viruses X, Y, S and M in meristem and explant cultures of potato in the presence of Virazole. Potato Res. 1982;(25):165-173.
15. Vlasov Yu.I., Larina E.I., Truskinov E.V. Agricultural phytovirusology St. Petersburg- Pushkin: VIZR, 2016. 236 p. (In Russ.)
16. Lapshinov N.A., Kulikova V.I., Ryabtseva T.V., etc. Technology of improvement and accelerated reproduction of potatoes (methodical manual). FGBNU "Kemerovo Research Institute". Kemerovo, 2014. 44 p. (In Russ.)
17. Oves E.V., Gabitova N.A. New elements of technology for improving and obtaining basic clones of promising potato varieties and hybrids. Achievements of science and technology of the agro-industrial complex. 2016;30(11):60-62. (In Russ.)
18. Mahmoud S.Y.M., Hosseny M.H., Abdel-Ghaffar M.H. Evaluation of some therapies to eliminate Potato Y potivirus from potato plants. Int. J. Virol. 2009;5(2):64-76. https://doi.org/10.3923/ijv.2009.64.76
19. Bădărău C.L., Chiru N. Effect of some therapies on potato plantlets infected with potato virus X (PVX). Journal of EcoAgriTourism. 2014;10(1/28):11-17.
20. The system of conducting agro-industrial production of Primorsky Krai / RASKHN, DV NMC, PRIMNIISKH. Novosibirsk. 2001. 364 p. (In Russ.)
21. GOST 33996-2016 Seed potatoes. Technical conditions and methods for determining quality. Introduction. 2018.01-01. Moscow: Standartinform. 2017. 41 p. (In Russ.)
22. Diagnostics of viruses and bacterioses in the process of improvement and reproduction of seed potatoes in the original seed production : method. recommendations. Comp. V.I. Kulikova et al. ; Kemerovo Research Institute. Kemerovo, 2008. 36 p. (In Russ.)
23. Bekesiova I., Nap J.-P., Mlynarova L. Isolation of high quality DNA and RNA from leaves of the carnivorous plant Drosera rotundifolia. Plant Mol. Biol. Rep. 1999;(17):269-277.
24. Barsukova E.N., Chekushkina T.N., Kim I.V. Improvement and micro-reproduction in vitro of potato varieties for virus-free seed production. Far East. agrarian. vestn. 2018;4(48):20-26. (In Russ.)
25. Yuryeva N.O., Voronkova E.V., Tereshonok D.V. et al. Introduction to the aseptic culture of potato digaploids using adventitious shoots and chemotherapy. Potato protection. 2017;(2):23-27. (In Russ.)
26. Ryabtseva T.V., Kulikova V.I., Khodaeva V.P. Evaluation of nutrient media during propagation of potato varieties in culture in vitro. International Research Journal. 2018;12(66)3:134-137. (In Russ.) https://doi.org/10.23670/IRJ.2017.66.099
27. Dhital S.P., Sakha B.M., Lim H.T. Utilization of shoot cuttings for elimination of PLRV and PVY by thermotherapy and chemotherapy from potato (Solanum tuberosum L.). Nepal J. Sci. Technol. 2006;(7):1-6.
28. Bespalova E.S., Agakhanov M.M., Arkhimandritova S.B., Erastenkova M.V., Uhatova Yu.V. Sanitization of potato varieties from the VIR collection against viruses. Proceedings on applied botany, genetics and breeding. 2020;181(4):164-172. (In Russ.) https://doi.org/10.30901/2227-8834-2020-4-164-172
Review
For citations:
Kim I.V., Shishchenko E.V., Fisenko P.V., Chibizova A.S., Klykov A.G. Biotechnology methods in virus-free potato seed production. Vegetable crops of Russia. 2022;(5):29-34. (In Russ.) https://doi.org/10.18619/2072-9146-2022-5-29-34
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