DNA technologies (molecular marking) in tomato breeding for resistance to Tobacco Mosaic Virus
https://doi.org/10.18619/2072-9146-2021-4-34-41
Abstract
Relevance. The purpose of this work is to create new discrete competitive tomato genotypes based on modern biotechnological approaches with increased resistance to the tobacco mosaic virus, economically valuable traits and adapted to the soil and climatic conditions of cultivation in the southern regions of the Russian Federation.
Methods. At the first stage of the study, SSR markers taken from literature sources were tested [1;2] and PCR conditions were optimized for the identification of target Tm genes (Tm2a, Tm22, providing resistance to tobacco mosaic virus at the tissue level) in the breeding material available in the department of vegetable and potato growing.
Results. Two informative molecular markers Tms 37 and UMD 2060 were selected, which reveal the allelic difference between resistant and susceptible samples. Hybridization of FMS tomato lines with samples having the desired genes of interest in the genotype for obtaining tomato lines resistant to TMV was carried out. Seeds of paternal forms - donors of target genes were obtained for their further use in breeding work. The scientific novelty lies in the study of the discrete genetic material of Solanumlycopersicum, used in the breeding process, and preserving its functions in a new genetic environment.
About the Authors
O. L. Gorun
Federal State Budgetary Institution "Federal Research Center of Rice"
Russian Federation
Russian Federation
Olesya L. Gorun – Junior Researcher of the Laboratory of Information, Digital and Biotechnologies
Federal State Budgetary Institution "Federal Research Center of Rice"
E. V. Dubina
Federal State Budgetary Institution "Federal Research Center of Rice"
Russian Federation
Russian Federation
Elena V. Dubina – Doc. Sci. (Biology), Head of the Laboratory of Information, Digital and Biotechnologies
3, Belozerny, Krasnodar
I. V. Kozlova
Federal State Budgetary Institution "Federal Research Center of Rice"3, Belozerny, Krasnodar
Russian Federation
Russian Federation
Irina V. Kozlova – Researcher of the Department of vegetable and potato growing
3, Belozerny, Krasnodar
I. V. Balyasny
Federal State Budgetary Institution "Federal Research Center of Rice"3, Belozerny, Krasnodar
Russian Federation
Russian Federation
Ivan V. Balyasny – Deputy Director
3, Belozerny, Krasnodar
S. V. Garkusha
Federal State Budgetary Institution "Federal Research Center of Rice"3, Belozerny, Krasnodar
Russian Federation
Russian Federation
Sergey V. Garkusha – Doc. Sci. (Agriculture), Professor, Acting Director
3, Belozerny, Krasnodar
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Review
For citations:
Gorun O.L., Dubina E.V., Kozlova I.V., Balyasny I.V., Garkusha S.V. DNA technologies (molecular marking) in tomato breeding for resistance to Tobacco Mosaic Virus. Vegetable crops of Russia. 2021;(4):34-41. (In Russ.) https://doi.org/10.18619/2072-9146-2021-4-34-41
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