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Получение удвоенных гаплоидов Cucurbita pepo L.

https://doi.org/10.18619/2072-9146-2021-4-11-26

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Аннотация

Удвоенные гаплоиды уже около 100 лет используются во всем мире в селекционных программах и фундаментальных исследованиях как ценный гомозиготный материал. Вид Cucurbita pepo L. представлен огромным многообразием форм, включает высокопродуктивные овощные культуры и имеет широкое распространение в мире. Несмотря на большую экономическую значимость, создание эффективных протоколов, обеспечивающих стабильное получение удвоенных гаплоидов у этого вида остается актуальной задачей. Получение DH-растений представляет интерес не только по причине ускорения селекционного процесса, но и за счет реализации огромного потенциала гаметоклональной изменчивости, заложенной у этого высоко полиморфного вида. В обзоре рассмотрены основные использующиеся технологии получения удвоенных гаплоидов у овощных культур C. pepo: партеногенез in situ стимулированный обработанной/облученной пыльцой, гиногенез in vitro (культура неопыленных семяпочек in vitro) и андрогенез in vitro (культура пыльников/микроспор in vitro). Представлен анализ исследований, проведенных с начала открытия гаплоидных растений до современных достижений и оценки перспектив в области получения DH растений. Рассмотрены основные критические факторы, влияющие на эффективность каждой из технологий и ее отдельных этапов. Представлена разработанная технология получения удвоенных гаплоидов кабачка с использованием культуры неопыленных семяпочек in vitro, позволяющая получать до 55 эмбриоидов на 1 культивируемую завязь (28 эмбриоидов/100 культивируемых семяпочек). Для внедрения гаплоидных технологий в селекционный процесс необходимо полученные растения-регенеранты оценивать на уровень плоидности. Использование прямого подсчета хромосом в апикальных клетках может представлять определенную сложность у этого вида ввиду большого их количества (2n=40) и их малого размера. В зависимости от уровня оснащения лаборатории определение плоидности с использованием проточной цитометрии клеточных ядер и подсчета количества хлоропластов в замыкающих клетках устьиц в эпителии абаксиальной стороны листа, может быть более удобными методами. В обзоре рассмотрены перспективы использования молекулярных маркеров для оценки на гомозиготность при DH-технологиях, в том числе и у C. pepo.

Об авторах

Е. А. Домблидес
Федеральное государственное бюджетное научное учреждение "Федеральный научный центр овощеводства" (ФГБНУ ФНЦО)
Россия

Елена Алексеевна Домблидес – кандидат с.-х. наук, зав. лаб. репродуктивной биотехнологии в селекции с.-х. растений

143072, Россия, Московская область, Одинцовский район, п. ВНИИССОК, ул. Селекционная, д. 14



Е. А. Ермолаев
Федеральное государственное бюджетное научное учреждение "Федеральный научный центр овощеводства" (ФГБНУ ФНЦО)
Россия

Алексей Станиславович Ермолаев - младший научный сотрудник лаборатории репродуктивной биотехнологии в селекции сельскохозяйственных растений

143072, Россия, Московская область, Одинцовский район, п. ВНИИССОК, ул. Селекционная, д.14



С. Н. Белов
Федеральное государственное бюджетное научное учреждение "Федеральный научный центр овощеводства" (ФГБНУ ФНЦО)
Россия

Сергей Николаевич Белов - младший научный сотрудник лаборатории репродуктивной биотехнологии в селекции сельскохозяйственных растений

143072, Россия, Московская область, Одинцовский район, п. ВНИИССОК, ул. Селекционная, д.14



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Рецензия

Для цитирования:


Домблидес Е.А., Ермолаев Е.А., Белов С.Н. Получение удвоенных гаплоидов Cucurbita pepo L. Овощи России. 2021;(4):11-26. https://doi.org/10.18619/2072-9146-2021-4-11-26

For citation:


Domblides E.A., Ermolaev A.S., Belov S.N. Obtaining doubled haploids of Cucurbita pepo L. Vegetable crops of Russia. 2021;(4):11-26. (In Russ.) https://doi.org/10.18619/2072-9146-2021-4-11-26

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