Preview

Vegetable crops of Russia

Advanced search

Marker-assisted breeding of onion (A. cepa L.) maintainer line resistant to Downy mildew

https://doi.org/10.18619/2072-9146-2021-3-34-39

Abstract

Relevance. F1 hybrids breeding programs of onion are based on the use of nuclear cytoplasmic male sterility (NCMS). Breeding and seed production based on NCMS suggest a development of a female sterile maternal line, a maintainer line and a paternal component. The development of an isogenic pair of sterile line – maintainer line is one of the most labor-, time-consuming and intellectually-intensive stages of a breeding program, the implementation of which becomes more complicated with an increase in the number of traits / genes for which selection is carried out.

Methods and results. This paper presents a genetic scheme for the development of a Downy mildewresistant (pathogen P. destructor) maintainer line of onion using marker-assisted selection (MAS). F2 populations were obtained from the cross of the female maintainer inbred line, Bn1- (13) (genotype – cytN msms pdpd) and male inbred line resistant to Downy mildew, No. 136 (genotype – cytT MsMs PdPd). DNA marker DMR1 were used for Downy mildew resistance gene Pd, jnurf13 – for maintainer gene ms and marker system 5`cob: orfA501 – for N, S and T cytoplasms. It was shown that the MAS application allow to develop a stable Downy mildew resistant maintainer line cytN msms PdPd in two generations.

About the Authors

Ya. T. Eidlin
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy (RSAU-MTAA)
Russian Federation

Yakov T. Eidlin – PhD-student, Department Botany, Plant Breeding and Seed Technology

49, Timiryazevskaya st., Moscow, 127550



G. F. Monakhos
Limited company «Breeding station after N. N. Timofeev»
Russian Federation

Grigory F. Monakhos – Cand. Sci. (Agriculture), General Director

5, Pasechnaya st., Moscow, 127550



S. G. Monakhos
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy (RSAU-MTAA)
Russian Federation

Sokrat G. Monakhos – Dr. Sci. (Agriculture), Head of the Department Botany, Plant Breeding and Seed Technology

49, Timiryazevskaya st., Moscow, 127550



References

1. Collard B. C. Y., Mackill D. J. Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Philosophical Transactions of the Royal Society B: Biological Sciences. 2008.;363(1491):557-572. https://doi.org/10.1098/rstb.2007.2170

2. Narasimhulu R. et al. Genetic variability and association studies for yield attributes in mungbean (Vigna radiata L. Wilczek). Indian J. Plant Sci. 2013;2(3):82-86.

3. Brumlop S., Finckh M.R. Applications and potentials of marker assisted selection (MAS) in plant breeding. Final report of the F+ E project “Applications and Potentials of Smart Breeding” (FKZ 350 889 0020) On behalf of the Federal Agency for Nature Conservation December. 2010.

4. Murovec N., Erker R.S., Prodan I. Determinants of environmental investments: testing the structural model. Journal of Cleaner Production. 2012;(37):265-277. https://doi.org/10.1016/j.jclepro.2012.07.024

5. Clarke, A.E., Jones H.A., Little T.M. Inheritance of bulb color in onion. Genetics. 1944;(29):569–575.

6. Berninger E. Contribution a l'etude de la sterilite male de l'oignon (Allium cepa L.). Ann. Amelior. Plant (Paris). 1965;(23):183–199.

7. Engelke T., Terefe D., Tatlioglu T. A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L.). Theoretical and Applied Genetics. 2003;107(1):162-167. https://doi.org/10.1007/s00122-003-1230-3

8. Havey M.J. Seed yield, floral morphology, and lack of male-fertility restoration of male-sterile onion (Allium cepa) populations possessing the cytoplasm of Allium galanthum. Journal of the American Society for Horticultural Science. 1999;124(6):626-629.

9. Yarwood C. E. et al. Onion downy mildew. Hilgardia. 1943;14(11).

10. Jesperson G.D., Sutton J.C. Evaluation of a forecaster for downy mildew of onion (Allium cepa L.). Crop protection. 1987;6(2):95-103.

11. Demidov E.S. Methods of protecting onions from false powdery mildew (Peronospora destructor Berk.). Tiraspol: "Tipar", 2004. 92 p.

12. Jones H.A., Mann L.K. Onions and their allies. Soil Science. 1964;98(1):68.

13. Kofoet A. et al. Inheritance of resistance to downy mildew (Peronospora destructor [Berk.] Casp.) from Allium roylei Stearn in the backcross Allium cepa L.×(A. roylei × A. cepa). Plant Breeding. 1990;105(2):144-149.

14. Khrustaleva L. et al. The power of genomic in situ hybridization (GISH) in interspecific breeding of bulb onion (Allium cepa L.) resistant to downy mildew (Peronospora destructor [Berk.] Casp.). Plants. 2019;8(2):36. https://doi.org/10.3390/plants8020036

15. Scholten O.E., Van Heusden A.W., Khrustaleva L.I., Burger-Meijer K., Mank R.A., Antonise R.G.C., Harrewijn J.L., Van haecke W., Oocst E.H., Peters R.J. The long and winding road leading to the successful introgression of downy mildew resistance into onion. Euphytica 2007;(156):345–353. https://doi.org/10.1007/s10681-007-9383-9

16. Kim S. et al. Development of a simple PCR marker tagging the Allium roylei fragment harboring resistance to downy mildew (Peronospora destructor) in onion (Allium cepa L.). Euphytica. 2016;208(3):561-569. https://doi.org/10.1007/s10681-015-1601-2

17. Kim S. A codominant molecular marker in linkage disequilibrium with a restorer-of-fertility gene (Ms) and its application in reevaluation of inheritance of fertility restoration in onions. Mol. Breeding. 2014;(34):769-778. https://doi.org/10.1007/s11032-014-0073-8

18. Rogers S.O., Bendich A.J. Extraction of DNA from plant tissues. Plant molecular biology manual. Springer, Dordrecht, 1989. P.73-83.


Review

For citations:


Eidlin Ya.T., Monakhos G.F., Monakhos S.G. Marker-assisted breeding of onion (A. cepa L.) maintainer line resistant to Downy mildew. Vegetable crops of Russia. 2021;(3):34-39. (In Russ.) https://doi.org/10.18619/2072-9146-2021-3-34-39

Views: 1021


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2072-9146 (Print)
ISSN 2618-7132 (Online)