Application of multifunctional compositions based on Rheum rhaponticum L. extracts for the elaboration of plant protection products
https://doi.org/10.18619/2072-9146-2020-4-98-101
Abstract
Relevance.Extract-based compositions, combining various types of biological activity were created and tested, to reveal the potential of genus Rheum (Polygonaceae)plants bioactive substances. The aim of the work was to determine the composition of bioactive substances in R. rhaponticum L. plants extracts and assess the possibility of their use as a means of protecting vegetable plants from powdery mildew.
Materials and methods. The methods of cultivation and research of R.rhaponticum plant materials was carried out at the Institute of Genetics, Physiology and Plant Protection, Chisinau, Republic of Moldova (2012-2019). The subject of our study the bioactive substances of rhubarb roots and leaves were used.
Results.The localization zones of anthracene derivatives were identified: in the main parenchyma of the secondary cortex and the parenchyma of the core rays of the rhubarb root. The amount of flavonoids and phenols in the root extract is 2 times more than in the leaf extract. To study the fungicidal and stimulating properties of rhubarb root extract in the control of powdery mildew, we conducted studies in a greenhouse on vegetable plants using artificial infection of seedlings. It was found that the chlorophyll transmittance index of leaves treated with rhubarb root extract increases until the concentration of the extract (R) reaches 1.5% (157.3 rel. units)(In the control – 119.2 rel. units). With increasing concentration of the extract to 2%, the chlorophyll index falls 1.2 times lower than the control values. The highest immunostimulating and fungicidal activity, intensifying with the addition of rhubarb leaf extract, was shown by rhubarb root extract. Its biological effectiveness was high at any intervals between treatment and infection (4 and 72 hours), and was directly proportional to concentration. Adding rhubarb leaf extract to the rhubarb root extract enhances the activity of the composition, and the risk of developing pesticide resistance among plant pathogens decreased.
About the Author
A. A. GladcaiaMoldova, Republic of
Alla A. Gladcaia – Cand.Sci. (Biology), Senior Researcher
Chisinau
References
1. Ui-jin Baea et al. Emodin isolated from Rheum palmatum prevents cytokine-induced β-cell damage and the development of type 1 diabetes. Journal of Functional Foods. 2015;(16):9-19. ISSN 1756-4646.
2. Bavaresco L. Role of viticultural factors on stilbene concentrations of grapes and wine. Drugs Under Experimental and Clinical Research. 2003;(29):181-187. ISSN 0378-6501.
3. Delmas D. et al. Resveratrol as a chemo preventive agent: a promising molecule for fighting cancer. Current Cancer Drug Targets. 2006;(7):423442. ISSN 15680096.
4. Das D., Maulik N. Resveratrol in cardioprotection: a therapeutic promise of alternative. Molecular Interventions. 2006;6(1):36-47. ISSN 15340384.
5. Matsuda H. Anti-allergic activity of stilbenes from Korean rhubarb (Rheum undulatum L.): structure requirements for inhibition of antigeninduced degranulation and their effects on the release of TNF-alpha and IL-4 in RBL-2H3 cells. Bioorganic & Medicinal Chemistry. 2004;12(18):4871-4876. ISSN 14643391.
6. Филонова О.В. Технология комплексной переработки ревеня. Известия вузов. Пищевая технология. 2005;(5-6):67-69. ISSN 05793009. [Filonova O. V. Technology of complex processing of rhubarb. University proceedings, Food technology. 2005;(5-6):67-69. ISSN 05793009. (In Russ.)]
7. Кацерикова Н.В. Научные и практические основы технологии натуральных продуктов питания с использованием красящих экстрактов из растительного сырья. Автореферат диссертации на соискание ученой степени доктора технических наук. Москва. 2003: 403 р. [Katserikova N.V. Scientific and practical principles of the technology of natural food products using coloring extracts from plant materials. Abstract of dissertation for the degree of Doctor of Technical Sciences. Moscow. 2003: 403. (In Russ.)]
8. Kobe organic and Kobe non-organic: 10% Rheum officinale Baill plant extract: Efficacy on the tomato powdery mildew Oidium lycopersici - Semifield test. 2011: 14. (просмотрено 03.11.2015). Available online: https://www.kobe12sl.com/wp-content/uploads/2014/10/Efficacy-Report2.pdf
9. Rajkovic S. et al. VEgard – botanical fungicide. IV International Symposium „Agrosym“. 2013: 511-516. ISBN 9789995575137.
10. Зайцева Н.В. Фармакогностическое исследование и стандартизация корней щавеля конского (Rúmex confértus Willd.). Диссертация на соискание ученой степени кандидата фармацевтических наук. Самара. 2014: 140. [Zaitseva N.V. Pharmacognostic research and standardization of the roots of horse sorrel (Rúmex confértus Willd.). The dissertation for the degree of candidate of pharmaceutical sciences. Samara. 2014: 140. (In Russ.)]
11. Шмыгарева А.А. Экспериментально-теоретическое обоснование подходов к стандартизации сырья и лекарственных препаратов фармакопейных растений, содержащих антрагликозиды. Диссертация на соискание ученой степени доктора фармацевтических наук, Самарский Государственный Медицинский Университет. Самара. 2017: 149-153. [Shmygareva A.A. Experimental and theoretical substantiation of approaches to standardization of raw materials and pharmaceutical preparations of pharmacopeia plants containing anthraglycosides. Thesis for the degree of Doctor of Pharmaceutical Sciences, Samara State Medical University. 2017: 149-153. (In Russ.)]
12. European Pharmacopoeia. 7th edition. Strasbourg: EDQM. 2011. (про
13. смотрено 23.10.2017). Available online: https://archive.org/stream/EuropeanPharmacopoeia80/EuropeanPharmacopoeia-8-0_djvu.txt
14. European Pharmacopoeia. 8th edition. Strasbourg: EDQM. 2013. (просмотрено 23.10.2017). Available online: http://www.fptl.ru/biblioteka/farmakopei/evropeyskaya _farmakopeya_8_vol-1.pdf
15. Государственная фармакопея Российской Федерации XIII издание. Москва. 2015. Федеральная электронная медицинская библиотека. [Электронный ресурс]. Режим доступа: http://www.femb.ru/feml (просмотрено 04.05.2017)
16. Бобейкэ В. Фармакогнозия. Анализ лекарственного растительного сырья. Учебно-методическое пособие. Кишинэу, CEP USM. 2007: 100. [Bobeike V. Pharmacognosy. Analysis of medicinal plant materials. Teaching aid. Chisinau, CEP USM, 2007: 100. (In Russ.)]
17. Hai S., Marrone P. Plant pathogen inhibitor combinations and methods of use, Patent number: 8889197. 2014. Marrone Bio Innovations Incorporation. (просмотрено 03.01.2016). Available online: http://patents.justia.com/patent/8889197
18. HAI S., MARRONE P. Plant pathogen inhibitor combinations and methods of use, Patent number: 8883227. 2014. Marrone Bio Innovations Incorporation (просмотрено 05.12.2017). Available online: http://patents.justia.com/assignee/marrone-bio-innovations?page=2
19. Торопова Е.Ю., Кириченко А.А. Фитосанитарный экологический мониторинг: Методические указания. Новосибирск. 2012: 38. ISBN 978-5-94477-115-5. [Toropova E.Y., Kirichenko A.A. Phytosanitary environmental monitoring: guidelines. Novosibirsk. 2012: 38. ISBN 978-594477-115-5. (In Russ.)]
20. Койшыбаев М., Муминджанов Х. Методические указания по мониторингу болезней, вредителей и сорных растений на посевах зерновых культур. Анкара. 2016: 28. ISBN 978-92-5-409180-4. [Koyshybayev M., Muminjanov H. Methodological guidelines for monitoring diseases, pests and weeds in crops of grain crops. Ankara. 2016: 28. ISBN 978-925-409180-4. (In Russ.)]
21. Îndrumări metodice pentru testarea produselor chimice şi biologice de protecţie a plantelor de dăunători, boli şi buruieni în Republica Moldova” Sub redacţia generală a doctorului în ştiinţe Ion Lazari. Chişinău. 2002: 286. ISBN 9975-9597-3-3. [Methodical guidelines for testing chemical and biological products for the protection of plants from pests, diseases and weeds in the Republic of Moldova” Edited by Dr. Ion Lazari. Chisinau, 2002: 286. ISBN 9975-9597-3-3. (In Mold.)]
22. Доспехов Б.А. Методика полевого опыта (с основами статистической обработки результатов исследований). Москва: Агропромиздат. 1985: 351. ISBN 978-5-458-23540-2. [Dospekhov B.A. Methods of field experience (with the basics of statistical processing of research results). Moscow: Agropromizdat. 1985: 351. ISBN 978-5-458-23540-2. (In Russ.)]
23. Рязанова Л.Г. Основы статистического анализа результатов исследований в садоводстве. Учебно-методическое пособие. Краснодар: КубГАУ. 2013: 61. [Ryazanova L.G. Fundamentals of statistical analysis of research results in gardening. Teaching aid. Krasnodar: KubSAU. 2013: 61. (In Russ.)]
24. Kiefer I., Slusarenko A. The pattern of systemic acquired resistance induction within the Arabidopsis rosette in relation to the pattern of translocation. Plant Physiology. 2003;132(2):840-847. ISSN 0066-4294.
25. Konstantinidou-Doltsinis et al., Efficacy of Milsana, a formulated plant extract from Reynoutria sachalinensis, against powdery mildew of tomato (Leveillula taurica). Department of Pesticides Control and Phytopharmacy. Greece. 2005;(21):1-2.
Review
For citations:
Gladcaia A.A. Application of multifunctional compositions based on Rheum rhaponticum L. extracts for the elaboration of plant protection products. Vegetable crops of Russia. 2020;(4):97-101. (In Russ.) https://doi.org/10.18619/2072-9146-2020-4-98-101