ovoshchiОвощи РоссииVegetable crops of Russia2072-91462618-7132Федеральный научный центр овощеводства10.18619/2072-9146-2019-5-15-19ovoshchi-880Research ArticleСЕЛЕКЦИЯ И СЕМЕНОВОДСТВО СЕЛЬСКОХОЗЯЙСТВЕННЫХ РАСТЕНИЙBREEDING AND SEED PRODUCTION OF AGRICULTURAL CROPSПоиск генисточников признака стерильности у образцов лука репчатого с использованием ДНК маркеровSearching for sterility genes in bulb onion breeding accessions with the use of DNA markershttps://orcid.org/0000-0002-5617-9498ДомблидесА. С.DomblidesArtur S.

кандидат cс.-х. наук, зав. лабораторией генетики и цитологии

143072, Московская обл., Одинцовский р-н, п. ВНИИССОК, ул. Селекционная, д.14

Ph.D. in Agriculture, Head of Laboratory of Genetics and Cytology

Selectionaya St. 14, VNIISSOK, Odintsovo region, Moscow oblast, 143080

arthurdom@inbox.ruФГБНУ «Федеральный научный центр овощеводства» (ФГБНУ ФНЦО)РоссияFSBSI Federal Scientific Vegetable CenterRussian Federation201907112019051519Copyright © Домблидес А.С., 20192019Домблидес А.С.Domblides A.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vegetables.su/jour/article/view/880Актуальность

Актуальность. Стерильность является важнейшим качественным признаком, который крайне необходим при получении коммерческих гибридов. Генетические факторы, отвечающие за проявление стерильности, сегодня могут быть определены с использованием ДНК маркеров, где при оценке больших популяций необходима их надежность и эффективность. Оценка существующих систем маркирования позволит определить их возможности при оценке селекционного материала.

Материал и методы

Материал и методы. Из литературных источников были взяты наиболее используемые маркеры для определения стерильности и проанализирована их эффективность на 19 селекционных образцах лука репчатого (Allium cepa L.).

Результаты

Результаты. Гены цитоплазмы 5’cob, orf725 and orfA501, влияющие на проявление признака стерильности были идентифицированы у ряда образцов. Аллели локуса восстановителя фертильности Ms были также обнаружены. Четыре селекционных образца имели S тип цитоплазмы, девять образцов показали Т тип и шесть образцов были с нормальной цитоплазмой по причине отсутствия генов стерильности. В результате использования набора этих маркеров были выявлены все варианты сочетания аллелей цитоплазмы и ядра: один закрепитель стерильности, стерильная форма, и два восстановителя фертильности. Однако необходимо отметить, что не все маркеры были удобными для поиска генов стерильности. Так, маркеры, разработанные на основе полиморфизма хлоропластной ДНК стерильных растений не дали сходных результатов с анализом митохондриальных генов стерильности. В итоге, показана практическая возможность оценки селекционного материала на наличие генов, отвечающих за проявление признака стерильности с целью поиска линий необходимых для получения гибридов у лука репчатого.

Relevance

Relevance. Sterility is a very important trait that is indispensable for hybrid production. Genetic factor underlying in plant sterility can be now identified in large plant populations by DNA markers with high effectiveness and reliability. The evaluation of such markers enables to define their current applicability in breeding program.

Methods

Methods. The markers from different publications that had been successfully used were taken to test their effectiveness on 19 accessions of bulb onion (Allium cepa L.).

Results

Results. Mitochondrial genes 5’cob, orf725 and orfA501 and alleles of fertility restoring locus Ms were also identified. Four breeding accessions had S-cytoplasm, nine accessions were with T-cytoplasm and six shared normal cytoplasm not showing any sterility gene in the analysis. As a result of marker testing, the all compositions of the genes in cytoplasm and Ms alleles in nucleus affecting the sterility had been revealed, such as one sterility maintainer, one male sterile accession, and two fertility restorers. However, it should be noted that not all markers tested were in accordance with each other, where the markers originated from chloroplast DNA of did not confirmed the results obtained with those cytoplasm-origins. As it was shown the practical use of the set of markers makes it possible to reveal necessary accessions with required gene composition for hybrid production in bulb onion.

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The authors declare that there are no conflicts of interest present.