Researches conducted on lands Nikolayev state agricultural research station IIA NAAS in 2017- 2018. Soil of an experimental plot is chernozem southern weakly eroded clay loamy on loess’s, it is noted by high contents potassium, average – phosphorus and it is not enough provided by nitrogen. Climate of region – continental, is characterized sharp and repeated by fluctuations annual and month temperature of air, greater spare of heat and aridity. Agrotechnic in experiment was generally accepted for southern Steppe of Ukraine. Scheme of experience included three factors – a sowing periods: II ten-day period of October (winter), II ten-day period of November (underwinter), I ten-day period of April (spring); variants of fertilizers: without fertilizers (control), recommended dose (N60P60) and N30Р30 broadcast + N30Р30 with irrigated water; the modes of irrigation: 80-70-70% of minimum moisture-holding capacity and 90-80-70% of minimum moisture-holding capacity. It is shown that hyssop – valuable spicy-aromatic culture, which on their own biological particularity, requirements to soil-climatic conditions can be successfully grown in southern Steppe of Ukraine, providing high harvest of floral mass for use in medical pharmacology. Most productivity variety Marquis (at a rate of 28.4-28.5 c/hа dry cheese) provided in variant, where contributed 50% dose of fertilizers broadcast and 50% with irrigated water and winter sowing period of culture. Maximum contents of essential oil has fixed in same variant at mode irrigation 80-70-70% of minimum moisture-holding capacity, where it has formed 0.85 %. Contents of ascorbic acid in plant raw material varied from 100.4 before 104.9 mg%.