ovoshchiОвощи РоссииVegetable crops of Russia2072-91462618-7132Федеральный научный центр овощеводства10.18619/2072-9146-2025-1-88-91ovoshchi-2553Research ArticleСАДОВОДСТВО, ОВОЩЕВОДСТВО, ВИНОГРАДАРСТВО И ЛЕКАРСТВЕННЫЕ КУЛЬТУРЫHORTICULTURE, VEGETABLE PRODUCTION, VITICULTURE AND MEDICINAL CROPSВодный экстракт листьев Astilbe chinensis как потенциальное средство для замедления активности пищеварительных ферментовAqueous extract of Astilbe chinensis leaves as a potential medicine to slow activity of digestive enzymesСоколоваЕ. В.SokolovaE. V.

Екатерина Владимировна Соколова – кандидат биол. наук

117216, г. Москва, ул. Грина, д. 7

Ekaterina V. Sokolova – Cand. Sci. (Biology), Leading Researcher

7, Grin street, Moscow, 117216

eka9739@yandex.ruhttps://orcid.org/0000-0003-4642-651XКрольТ. А.KrolT. N.

Татьяна Анатольевна Кроль – кандидат с.-х. наук, ведущий научный сотрудник

117216, г. Москва, ул. Грина, д. 7

Tatiana A. Krol – Cand. Sci. (Agriculture), Leading Researcher

7, Grin street, Moscow, 117216

tatianakroll1@gmail.comhttps://orcid.org/0000-0002-1228-0594БалеевД. Н.BaleevD. N.

Дмитрий Николаевич Балеев – кандидат с.-х. наук, зав. лаб.

117216, г. Москва, ул. Грина, д. 7

Dmitry N. Baleev – Cand. Sci. (Agriculture)

7, Grin street, Moscow, 117216

dbaleev@gmail.comФедеральное государственное бюджетное научное учреждение «Всероссийский Институт Лекарственных и Ароматических Растений» (ФГБНУ ВИЛАР)РоссияAll-Russian scientific research Institute of medicinal and aromatic plantsRussian Federation202516012025018891Copyright © Соколова Е.В., Кроль Т.А., Балеев Д.Н., 20252025Соколова Е.В., Кроль Т.А., Балеев Д.Н.Sokolova E.V., Krol T.N., Baleev D.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vegetables.su/jour/article/view/2553Актуальность

Актуальность. Astilbe chinensis обладает клинической эффективностью в отношении многих заболеваний, что делает это растение потенциальным природным источником для лечения диабета и избыточного веса с мягким, неагрессивным воздействием. В целом, изучение биологических свойств листьев A. chinensis в настоящее время только начинается.

Целью исследования являлось расширение возможного спектра применения водного экстракта листьев A. chinensis в терапевтической практике человека.

Методы

Методы. Изучен анти-амилазный и анти-липазный потенциал водного экстракта из листьев астильбы китайской как важного представителя на роль природных комплексных веществ с терапевтическим потенциалом. Общее содержание фенольных соединений определяли методом Фолина-Чокальтеу и флавоноидов колориметрическим методом по реакции комплексообразования с хлоридом-алюминия. Влияние водных/чайных экстрактов листьев A. chinensis на панкреатические амилазу и липазу изучали в моделях in vitro в сравнении акарбозой и орлистатом, соответственно. Для этих тестов использовали искусственные субстраты сложный эфир 1,2-О-дилаурил-рац-глицеро-3-глутаровой кислоты (6-метилрезоруфин) и 2-хлор-4-нитрофенол-олигосахарид.

Результаты

Результаты. Исследование влияния на панкреатические амилазу и липазу водным экстрактом листьев A. chinensis показало сравнимый ингибирующий эффект в 18% в отношении обоих ферментов. Заключение. Ингибирущий потенциал для водного экстаракта листьев A. chinensis в отношении амилазы и липазы позволяет предположить комплексное воздействие на ключевые ферменты дисфункционирующие при ряде метаболических нарушений человека.

Relevance

Relevance. Astilbe chinensis has clinical efficacy against many diseases, making this plant a potential natural source for treating diabetes and obesity with a mild, non-aggressive effect. Overall, the study of the biological properties of A. chinensis leaves is currently in its infancy.

Aim

Aim. In this study, the anti-amylase and anti-lipase potential of aqueous extract of Astilbe chinensis leaves was investigated as an important source of natural complex substances with therapeutic potential.

 Methods

 Methods. The total content of phenolic compounds was determined by the Folin-Ciocalteu method and flavonoids by the colorimetric method according to the complexation reaction with aluminum chloride. The effects of aqueous/tea extracts of A. chinensis leaves on pancreatic amylase and lipase were studied via in vitro models in comparison with acarbose and orlistat, respectively. For these tests, the artificial substrates 1,2-O-dilauryl-rac-glycero-3-glutaric acid ester (6-methylresorufin) and 2-chloro-4-nitrophenol oligosaccharide were used.

Results

Results. The study of the effect of aqueous extract of A. chinensis leaves on pancreatic amylase and lipase showed a comparable inhibitory effect of 18% on both enzymes.

Astilbe chinensisводный экстрактфенольные соединенияфлавоноидыамилазалипазаAstilbe chinensisводный экстрактфенольные соединенияфлавоноидыамилазалипазаРабота выполнена согласно Государственному заданию по теме FGUU 2022–0013.The work was carried out in accordance with the State Assignment on the topic FGUU 2022–0013.ReferencesSivajothi V., Dey .A, Jayakara B., Rajkapoor B. Antihyperglycemic, antihyperlipidemic and antioxidant effect of Phyllanthus rheedii on streptozotocin induced diabetic rats. Iranian Journal of Pharmaceutical Research. 2008;7(1):53-59.Sivajothi V., Dey .A, Jayakara B., Rajkapoor B. Antihyperglycemic, antihyperlipidemic and antioxidant effect of Phyllanthus rheedii on streptozotocin induced diabetic rats. Iranian Journal of Pharmaceutical Research. 2008;7(1):53-59.Kumar S., Mittal A., Babu D., Mittal A. Herbal medicines for diabetes management and its secondary complications. Current diabetes reviews. 2021;17(4):437-56. https://doi.org/10.2174/1573399816666201103143225Kumar S., Mittal A., Babu D., Mittal A. Herbal medicines for diabetes management and its secondary complications. Current diabetes reviews. 2021;17(4):437-56. https://doi.org/10.2174/1573399816666201103143225Singh J.K., Chakraborty S., Nagpal M., Aggarwal G. Herbal Approach for Diabetic Cure and Futuristic Dimension. Current Drug Research Reviews Formerly: Current Drug Abuse Reviews. 2023;15(3):207-21. https://doi.org/10.2174/2589977515666230217114449Singh J.K., Chakraborty S., Nagpal M., Aggarwal G. Herbal Approach for Diabetic Cure and Futuristic Dimension. Current Drug Research Reviews Formerly: Current Drug Abuse Reviews. 2023;15(3):207-21. https://doi.org/10.2174/2589977515666230217114449Seo C., Jeong W. Lee J.E., Kwon J.G., Kim J.K., Hong S.S. Flavonoids from the aerial parts of Astilbe rubra. Chemistry of Natural Compounds. 2019;55(6):1153-5. https://doi.org/10.1007/s10600-019-02919-wSeo C., Jeong W. Lee J.E., Kwon J.G., Kim J.K., Hong S.S. Flavonoids from the aerial parts of Astilbe rubra. Chemistry of Natural Compounds. 2019;55(6):1153-5. https://doi.org/10.1007/s10600-019-02919-wGil T.Y., Jin B.R., Hong C.H., Park J.H., An H.J. Astilbe Chinensis ethanol extract suppresses inflammation in macrophages via NF-κB pathway. BMC Complementary Medicine and Therapies. 2020;20:1-1. https://doi.org/10.1186/s12906-020-03073-5Gil T.Y., Jin B.R., Hong C.H., Park J.H., An H.J. Astilbe Chinensis ethanol extract suppresses inflammation in macrophages via NF-κB pathway. BMC Complementary Medicine and Therapies. 2020;20:1-1. https://doi.org/10.1186/s12906-020-03073-5Zhang Y., Wang J., Guo H. Ultrasound-assisted extraction of bergenin from Astilbe chinensis. Genetic resources and crop evolution. 2014;61:893-9. https://doi.org/10.1007/s10722-014-0117-2Zhang Y., Wang J., Guo H. Ultrasound-assisted extraction of bergenin from Astilbe chinensis. Genetic resources and crop evolution. 2014;61:893-9. https://doi.org/10.1007/s10722-014-0117-2Zhang X.H., Wang Z., Kang B.G., Hwang S.H., Lee J.Y., Lim S.S., Huang B. Antiobesity effect of Astilbe chinensis Franch. et savet. Extract through regulation of adipogenesis and AMP-activated protein kinase pathways in 3T3-L1 adipocyte and high-fat diet-induced C57BL/6N obese mice. Evidence - Based Complementary and Alternative Medicine. 2018;2018(1):1347612. https://doi.org/10.1155/2018/1347612Zhang X.H., Wang Z., Kang B.G., Hwang S.H., Lee J.Y., Lim S.S., Huang B. Antiobesity effect of Astilbe chinensis Franch. et savet. Extract through regulation of adipogenesis and AMP-activated protein kinase pathways in 3T3-L1 adipocyte and high-fat diet-induced C57BL/6N obese mice. Evidence - Based Complementary and Alternative Medicine. 2018;2018(1):1347612. https://doi.org/10.1155/2018/1347612Xue Y., Xu X.M., Yan J.F., Deng W.L., Liao X. Chemical constituents from Astilbe chinensis. Journal of Asian natural products research. 2011;13(02):188-91. https://doi.org/10.1080/10286020.2010.546355Xue Y., Xu X.M., Yan J.F., Deng W.L., Liao X. Chemical constituents from Astilbe chinensis. Journal of Asian natural products research. 2011;13(02):188-91. https://doi.org/10.1080/10286020.2010.546355Nho J.H., Jang J.H., Jung H.K., Lee M.J., Sim M.O., Jeong D.E., Cho H.W. Ethanol extracts from Astilbe chinensis (Maxim.) Franch. Et Savat. exhibit inhibitory activities on oxidative stress generation and viability of human colorectal cancer cells. Korean Journal of Medicinal Crop Science. 2018;26(2):141-7. https://doi.org/10.7783/KJMCS.2018.26.2.141Nho J.H., Jang J.H., Jung H.K., Lee M.J., Sim M.O., Jeong D.E., Cho H.W. Ethanol extracts from Astilbe chinensis (Maxim.) Franch. Et Savat. exhibit inhibitory activities on oxidative stress generation and viability of human colorectal cancer cells. Korean Journal of Medicinal Crop Science. 2018;26(2):141-7. https://doi.org/10.7783/KJMCS.2018.26.2.141Jeon B.R., Irfan M., Lee S.E., Lee J.H., Rhee M.H. Astilbe chinensis modulates platelet function via impaired MAPK and PLCγ2 expression. Evidence - Based Complementary and Alternative Medicine. 2018;2018(1):3835021. https://doi.org/10.1155/2018/3835021Jeon B.R., Irfan M., Lee S.E., Lee J.H., Rhee M.H. Astilbe chinensis modulates platelet function via impaired MAPK and PLCγ2 expression. Evidence - Based Complementary and Alternative Medicine. 2018;2018(1):3835021. https://doi.org/10.1155/2018/3835021Chen C., Yang M., Chen Y., Wang Y., Wang K., Li T., Hu Q., Zhang W., Xia J. Astilbin-induced inhibition of the PI3K/AKT signaling pathway decelerates the progression of osteoarthritis. Experimental and Therapeutic Medicine. 2020;20(4):3078-83. https://doi.org/10.3892/etm.2020.9048Chen C., Yang M., Chen Y., Wang Y., Wang K., Li T., Hu Q., Zhang W., Xia J. Astilbin-induced inhibition of the PI3K/AKT signaling pathway decelerates the progression of osteoarthritis. Experimental and Therapeutic Medicine. 2020;20(4):3078-83. https://doi.org/10.3892/etm.2020.9048Sancheti S., Sancheti S., Lee S.H., Lee J.E., Seo S.Y. Screening of Korean medicinal plant extracts for α-glucosidase inhibitory activities. Iranian journal of pharmaceutical research: IJPR. 2011;10(2):261. PMID: 24250352Sancheti S., Sancheti S., Lee S.H., Lee J.E., Seo S.Y. Screening of Korean medicinal plant extracts for α-glucosidase inhibitory activities. Iranian journal of pharmaceutical research: IJPR. 2011;10(2):261. PMID: 24250352Lim J.S., Kyung S.Y., Jeon Y., Kim I.S., Kwak J.H., Kim H.S. Anticancer effects of the HDAC inhibitor, 3β, 6β dihydroxyurs 12 en 27 oic acid, in MCF 7 breast cancer cells via the inhibition of Akt/mTOR pathways. Oncology Reports. 2023;49(2):1-2. https://doi.org/10.3892/or.2023.8480Lim J.S., Kyung S.Y., Jeon Y., Kim I.S., Kwak J.H., Kim H.S. Anticancer effects of the HDAC inhibitor, 3β, 6β dihydroxyurs 12 en 27 oic acid, in MCF 7 breast cancer cells via the inhibition of Akt/mTOR pathways. Oncology Reports. 2023;49(2):1-2. https://doi.org/10.3892/or.2023.8480Han J.M., Yun I., Yang K.M., Kim H.S., Kim Y.Y., Jeong W., Hong S.S., Hwang I. Ethanol extract from Astilbe chinensis inflorescence suppresses inflammation in macrophages and growth of oral pathogenic bacteria. Plos one. 2024;19(7):e0306543. https://doi.org/10.1371/journal.pone.0306543Han J.M., Yun I., Yang K.M., Kim H.S., Kim Y.Y., Jeong W., Hong S.S., Hwang I. Ethanol extract from Astilbe chinensis inflorescence suppresses inflammation in macrophages and growth of oral pathogenic bacteria. Plos one. 2024;19(7):e0306543. https://doi.org/10.1371/journal.pone.0306543Singleton V.L., Rossi J.A. Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. American journal of Enology and Viticulture. 1965;16(3):144-58. https://doi.org/10.5344/ajev.1965.16.3.144Singleton V.L., Rossi J.A. Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. American journal of Enology and Viticulture. 1965;16(3):144-58. https://doi.org/10.5344/ajev.1965.16.3.144Ojha S., Raj A., Roy A., Roy S. Extraction of total phenolics, flavonoids and tannins from Paederia foetida L. Leaves and their relation with antioxidant activity. Pharmacognosy Journal. 2018;10(3). https://doi.org/10.5530/pj.2018.3.88Ojha S., Raj A., Roy A., Roy S. Extraction of total phenolics, flavonoids and tannins from Paederia foetida L. Leaves and their relation with antioxidant activity. Pharmacognosy Journal. 2018;10(3). https://doi.org/10.5530/pj.2018.3.88Chang C.C., Yang M.H., Wen H.M., Chern J.C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis. 2002;10(3).Chang C.C., Yang M.H., Wen H.M., Chern J.C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis. 2002;10(3).Mammen D., Daniel M. A critical evaluation on the reliability of two aluminum chloride chelation methods for quantification of flavonoids. Food chemistry. 2012;135(3):1365-8. https://doi.org/10.1016/j.foodchem.2012.05.109Mammen D., Daniel M. A critical evaluation on the reliability of two aluminum chloride chelation methods for quantification of flavonoids. Food chemistry. 2012;135(3):1365-8. https://doi.org/10.1016/j.foodchem.2012.05.109Morishita Y., Iinuma Y., Nakashima N., Majima K., Mizuguchi K., Kawamura Y. Total and pancreatic amylase measured with 2-chloro-4-nitrophenyl-4-O-β-D-galactopyranosylmaltoside. Clinical chemistry. 2000;46(7):928-33. https://doi.org/10.1093/clinchem/46.7.928Morishita Y., Iinuma Y., Nakashima N., Majima K., Mizuguchi K., Kawamura Y. Total and pancreatic amylase measured with 2-chloro-4-nitrophenyl-4-O-β-D-galactopyranosylmaltoside. Clinical chemistry. 2000;46(7):928-33. https://doi.org/10.1093/clinchem/46.7.928Panteghini M., Bonora R., Pagani F. Measurement of pancreatic lipase activity in serum by a kinetic colorimetric assay using a new chromogenic substrate. Annals of clinical biochemistry. 2001;38(4):365-70. https://doi.org/10.1258/0004563011900876Panteghini M., Bonora R., Pagani F. Measurement of pancreatic lipase activity in serum by a kinetic colorimetric assay using a new chromogenic substrate. Annals of clinical biochemistry. 2001;38(4):365-70. https://doi.org/10.1258/0004563011900876Zhang Y., Xie J. Targeting ferroptosis regulators by natural products in colorectal cancer. Frontiers in Pharmacology. 2024;15:1374722. https://doi.org/10.3389/fphar.2024.1374722Zhang Y., Xie J. Targeting ferroptosis regulators by natural products in colorectal cancer. Frontiers in Pharmacology. 2024;15:1374722. https://doi.org/10.3389/fphar.2024.1374722Kumar D.A., Anusha S.V., Oruganti S., Deshpande M., Zehra A., Tiwari A.K. Raw versus cooked vegetable juice: Effect on parameters of glycaemic overload and oxidative stress in vitro. Nutrafoods. 2015;14:27-38. https://doi.org/10.1007/s13749-014-0066-6Kumar D.A., Anusha S.V., Oruganti S., Deshpande M., Zehra A., Tiwari A.K. Raw versus cooked vegetable juice: Effect on parameters of glycaemic overload and oxidative stress in vitro. Nutrafoods. 2015;14:27-38. https://doi.org/10.1007/s13749-014-0066-6Tiwari A.K., Jyothi A.L., Tejeswini V.B., Madhusudana K., Kumar D.A., Zehra A., Agawane S.B. Mitigation of starch and glucose-induced postprandial glycemic excursion in rats by antioxidant-rich green-leafy vegetables’ juice. Pharmacognosy Magazine. 2013;9(Suppl1):S66. https://doi.org/10.4103/0973-1296.117872Tiwari A.K., Jyothi A.L., Tejeswini V.B., Madhusudana K., Kumar D.A., Zehra A., Agawane S.B. Mitigation of starch and glucose-induced postprandial glycemic excursion in rats by antioxidant-rich green-leafy vegetables’ juice. Pharmacognosy Magazine. 2013;9(Suppl1):S66. https://doi.org/10.4103/0973-1296.117872Aghajanyan A., Nikoyan A., Trchounian A. Biochemical activity and hypoglycemic effects of Rumex obtusifolius L. seeds used in Armenian traditional medicine. BioMed Research International. 2018;2018(1):4526352. https://doi.org/10.1155/2018/4526352Aghajanyan A., Nikoyan A., Trchounian A. Biochemical activity and hypoglycemic effects of Rumex obtusifolius L. seeds used in Armenian traditional medicine. BioMed Research International. 2018;2018(1):4526352. https://doi.org/10.1155/2018/4526352Podsedek A., Majewska I., Kucharska A.Z. Inhibitory potential of red cabbage against digestive enzymes linked to obesity and type 2 diabetes. Journal of Agricultural and Food Chemistry. 2017;65(33):7192-9. https://doi.org/10.1021/acs.jafc.7b02499Podsedek A., Majewska I., Kucharska A.Z. Inhibitory potential of red cabbage against digestive enzymes linked to obesity and type 2 diabetes. Journal of Agricultural and Food Chemistry. 2017;65(33):7192-9. https://doi.org/10.1021/acs.jafc.7b02499Savran A., Zengin G., Aktumsek A., Mocan A., Glamoćlija J., Ćirić A., Soković M. Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits. Food & function. 2016;7(7):3252-62. https://doi.org/10.1039/C6FO00695GSavran A., Zengin G., Aktumsek A., Mocan A., Glamoćlija J., Ćirić A., Soković M. Phenolic compounds and biological effects of edible Rumex scutatus and Pseudosempervivum sempervivum: potential sources of natural agents with health benefits. Food & function. 2016;7(7):3252-62. https://doi.org/10.1039/C6FO00695GSpínola V., Llorent-Martínez E.J., Castilho P.C. Inhibition of α-amylase, α-glucosidase and pancreatic lipase by phenolic compounds of Rumex maderensis (Madeira sorrel). Influence of simulated gastrointestinal digestion on hyperglycaemia-related damage linked with aldose reductase activity and protein glycation. Lwt. 2020;118:108727. https://doi.org/10.1016/j.lwt.2019.108727Spínola V., Llorent-Martínez E.J., Castilho P.C. Inhibition of α-amylase, α-glucosidase and pancreatic lipase by phenolic compounds of Rumex maderensis (Madeira sorrel). Influence of simulated gastrointestinal digestion on hyperglycaemia-related damage linked with aldose reductase activity and protein glycation. Lwt. 2020;118:108727. https://doi.org/10.1016/j.lwt.2019.108727Kim H.Y., Lim S.H., Park Y.H., Ham H.J., Lee K.J., Park D.S., Kim K.H., Kim S.M. Screening of α-amylase, α-glucosidase and lipase inhibitory activity with Gangwon-do wild plants extracts. Journal of the Korean Society of Food Science and Nutrition. 2011;40(2):308-15. https://doi.org/10.3746/jkfn.2011.40.2.308Kim H.Y., Lim S.H., Park Y.H., Ham H.J., Lee K.J., Park D.S., Kim K.H., Kim S.M. Screening of α-amylase, α-glucosidase and lipase inhibitory activity with Gangwon-do wild plants extracts. Journal of the Korean Society of Food Science and Nutrition. 2011;40(2):308-15. https://doi.org/10.3746/jkfn.2011.40.2.308Kumar A., Chauhan S.. Pancreatic lipase inhibitors: The road voyaged and successes. Life Sciences. 2021;271:119115. https://doi.org/10.1016/j.lfs.2021.119115Kumar A., Chauhan S.. Pancreatic lipase inhibitors: The road voyaged and successes. Life Sciences. 2021;271:119115. https://doi.org/10.1016/j.lfs.2021.119115

The authors declare that there are no conflicts of interest present.