ovoshchiОвощи РоссииVegetable crops of Russia2072-91462618-7132Федеральный научный центр овощеводства10.18619/2072-9146-2024-4-23-27ovoshchi-2445Research ArticleСАДОВОДСТВО, ОВОЩЕВОДСТВО, ВИНОГРАДАРСТВО И ЛЕКАРСТВЕННЫЕ КУЛЬТУРЫAGROCHEMISTRY, SOIL SCIENCE, PLANT PROTECTION AND QUARANTINEСравнительная оценка эффективности защиты Raphanus sativus var. lobo от крестоцветной галлицы (Contarinia nasturtii), используя внекорневое внесение селената натрия, ионной формы кремния и экстракта чеснокаComparative evaluation of Raphanus sativus var. lobo defense efficiency against Contarinia nasturtii using foliar application of sodium selenate, ionic silicon form and garlic extracthttps://orcid.org/0000-0003-1803-9168ГолубкинаН. А.GolubkinaN. А.

Надежда Александровна Голубкина – доктор с.-х. наук, главный научный сотрудник лабораторно-аналитического отдела

143072, Московская область, Одинцовский район, п. ВНИИССОК, ул. Селекционная, д.14

Nadezhda A. Golubkina – Dr. Sci. (Agriculture), Chief Researcher of Laboratory-Analytical Department

14, Selectsionnaya str., VNIISSOK, Odintsovo district, Moscow region, 143072

segolubkina45@gmail.comhttps://orcid.org/0000-0001-9821-5381ЗаячковскийВ. А.ZayachkovskyV. А.

Владимир Александрович Заячковский – кандидат с.-х. наук, старший научный сотрудник лаборатория селекции и семеноводства корнеплодных культур

143072, Московская область, Одинцовский район, п. ВНИИССОК, ул. Селекционная, д.14

Vladimir A. Zayachkovsky – Cand. Sci. (Agriculture), Senior Researcher, Laboratory of Breeding and Seed Production of Root Crops

14, Selectsionnaya str., VNIISSOK, Odintsovo district, Moscow region, 143072

vladimir898542178114@mail.ruФедеральное государственное бюджетное научное учреждение "Федеральный научный центр овощеводства" (ФГБНУ ФНЦО)РоссияFederal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC)Russian Federation202409072024042327Copyright © Голубкина Н.А., Заячковский В.А., 20242024Голубкина Н.А., Заячковский В.А.Golubkina N.А., Zayachkovsky V.А.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vegetables.su/jour/article/view/2445Актуальность

Актуальность. Крестоцветная галлица Contarinia nasturtii Keiffer – один из наиболее опасных вредителей растений семейства Brassicaceae, снижающих семенную продуктивность растений.

Материал и методика

Материал и методика. Исследована эффективность однократного внекорневого внесения селената натрия, ионной формы кремния (удобрение Силиплант) и экстракта чеснока на эффективность защиты растений лобо Raphanus sativus lobo от Contarinia nasturtii в условиях закрытого грунта. Используемые концентрации: селена 26.4 мМ раствор, Силипланта – 1 мл/л, экстракта чеснока – 8 г/л. Проводили оценку семенной продуктивности и уровней инфицирования растений крестоцветной галлицей, а также определяли показатели антиоксидантного статуса растений и содержания сахаров.

Результаты

Результаты. Использование Силипланта без и совместно с селенатом натрия обеспечивало наибольшую семенную продуктивность растений лобо и наибольшую массу 1000 семян. Обработка семенных растений кремнием, экстрактом чеснока и совместное применение кремния и селена обеспечивали 100% защиту растений от C. nasturtii уже через неделю после обработки. Напротив, применение селената натрия обеспечивало лишь частичную защиту. Среди исследованных биохимических показателей уровень водорастворимых соединений (TDS) был наибольшим у растений, обработанных селеном без и на фоне использования кремния. Изменения в интенсивности защиты растений от крестоцветной галлицы благодаря использованию экстракта чеснока и растворов селената натрия и Силипланта отражались в возрастании уровней накопления полифенолов в листьях по сравнению с данными для контрольных полностью инфицированных растений и в значительном возрастании накопления дисахаридов, участвующих в восстановлении тканей после атаки вредных насекомых. Установлено также, что кремний стимулирует аккумулирование селена листьями растений как без, так и при обработке растений селенатом натрия.

Relevance

Relevance. Contarinia nasturtii Keiffer is one of the most dangerous insect pests of Brassicaceae plants dramatically affecting plant yield and seed productivity.

Material and Methods

Material and Methods. The effect of single foliar application of sodium selenate, ionic form of silicon (Siliplant fertilizer) and garlic extract on the efficiency of Raphanus sativus lobo defense against Contarinia nasturtii infestation was evaluated in condition of greenhouse. Concentrations of the applied reagents were: sodium selenate 26.4 mM; Siliplant – 1 ml/l; garlic extract – 8 g/l. Seed productivity and C. nasturtii infestation levels under Se, Si and garlic extract administration under infestation were determined along with the analysis of plant antioxidant status and sugar content.

Results

Results. Foliar application of Siliplant singular or in combination with Se provided the highest seed productivity and mass of 1000 seeds. Plants treated with Si, garlic extract and Si+Se demonstrated lack of C. nasturtii infestation only a week after the preparation supply. Contrary, selenate provided less pronounced defense. Among biochemical parameters tested the values of the total dissolved solids were the highest in plants treated with Se and Se+Si. Changes in the intensity of plant defense due to garlic, Se and Si supply were reflected in the increase of the total phenolic content compared to the values typical for control plants and in a significant accumulation increase of disaccharides, participating in tissue recovery after herbivory attack. Silicon application also provided an increase of selenium accumulation by lobo leaves.

Raphanus sativus var. loboContarinia nasturtiiселенкремнийэкстракт чесноказащита от вредных насекомыхRaphanus sativus var. loboContarinia nasturtiiseleniumsilicongarlic extractsprotection against pestsReferencesDarvas B., Skuhrava M., Andersen A. Agricultural Dipteran pests of the Palearctic region. In: Papp, L. and Darvas, B. (eds) Contributions to a Manual of Palearctic Diptera, with Special Reference to Flies of Economic Importance. Science Herald, Budapest, Hungary, 2000.Darvas B., Skuhrava M., Andersen A. Agricultural Dipteran pests of the Palearctic region. In: Papp, L. and Darvas, B. (eds) Contributions to a Manual of Palearctic Diptera, with Special Reference to Flies of Economic Importance. Science Herald, Budapest, Hungary, 2000.Hallett R.H., Heal J.D. First Nearctic record of the swede midge (Diptera: Cecidomyiidae), a pest of cruciferous crops from Europe. Can. Entomol. 2001;(133):713–715 https://doi.org/10.4039/ent133713-5Hallett R.H., Heal J.D. First Nearctic record of the swede midge (Diptera: Cecidomyiidae), a pest of cruciferous crops from Europe. Can. Entomol. 2001;(133):713–715 https://doi.org/10.4039/ent133713-5Wu Q.-J., Zhao J.-Z., Taylor A.G., Shelton A.M. Evaluation of insecticides and application methods against Contarinia nasturtii (Diptera: Cecidomyiidae), a new invasive insect pest in the United States. J. Economic Entomol. 2006;(99):117–122.Wu Q.-J., Zhao J.-Z., Taylor A.G., Shelton A.M. Evaluation of insecticides and application methods against Contarinia nasturtii (Diptera: Cecidomyiidae), a new invasive insect pest in the United States. J. Economic Entomol. 2006;(99):117–122.Chen M., Zhao J.-Z., Shelton A.M. Control of Contarinia nasturtii Kieffer (Diptera: Cecidomyiidea) by foliar sprays of acetamiprid on cauliflower transplants. Crop Protection. 2007;(26):1574–1578.Chen M., Zhao J.-Z., Shelton A.M. Control of Contarinia nasturtii Kieffer (Diptera: Cecidomyiidea) by foliar sprays of acetamiprid on cauliflower transplants. Crop Protection. 2007;(26):1574–1578.Hallett R.H., Chen M., Sears M.K., Shelton A.M. Insecticide management strategies for control of swede midge (Diptera: Cecidomyiidae) on cole crops. J. Economic Entomol. 2009;(102):2241–2254.Hallett R.H., Chen M., Sears M.K., Shelton A.M. Insecticide management strategies for control of swede midge (Diptera: Cecidomyiidae) on cole crops. J. Economic Entomol. 2009;(102):2241–2254.Abram P.K., Boivin G., Haye T., Mason P.G. Contarinia nasturtii Kieffer, Swede Midge (Diptera: Cecidomyiidae) Chapter 18 CAB International 2013. Biological Control Programs in Canada 2001–2012 (eds P.G. Mason and D.R. Gillespie).Abram P.K., Boivin G., Haye T., Mason P.G. Contarinia nasturtii Kieffer, Swede Midge (Diptera: Cecidomyiidae) Chapter 18 CAB International 2013. Biological Control Programs in Canada 2001–2012 (eds P.G. Mason and D.R. Gillespie).Rinaldi S., Casorri L., Masciarelli E., Ficociello B., Visconti U., Papetti P., Neri U., Beni C. Prospects of using garlic extracts for pest control in sustainable agriculture. Fresenius Environ. Bull. 2019;28(2):535-540.Rinaldi S., Casorri L., Masciarelli E., Ficociello B., Visconti U., Papetti P., Neri U., Beni C. Prospects of using garlic extracts for pest control in sustainable agriculture. Fresenius Environ. Bull. 2019;28(2):535-540.Golubkina N., Zayachkovsky V., Sheshnitsan S., Skrypnik L., Antoshkina M., Smirnova A., Fedotov M., Caruso G. Prospects of the application of garlic extracts and selenium and silicon compounds for plant protection against herbivorous pests: a review. Agriculture. 2022;(12):64. https://doi.org/10.3390/agriculture12010064Golubkina N., Zayachkovsky V., Sheshnitsan S., Skrypnik L., Antoshkina M., Smirnova A., Fedotov M., Caruso G. Prospects of the application of garlic extracts and selenium and silicon compounds for plant protection against herbivorous pests: a review. Agriculture. 2022;(12):64. https://doi.org/10.3390/agriculture12010064Li Q., Xian L., Yuan L., Lin Z., Chen X., Wang J., Li T. The use of selenium for controlling plant fungal diseases and insect pests. Front. Plant Sci. 2023;(14):1102594. https://doi.org/10.3389/fpls.2023.1102594.Li Q., Xian L., Yuan L., Lin Z., Chen X., Wang J., Li T. The use of selenium for controlling plant fungal diseases and insect pests. Front. Plant Sci. 2023;(14):1102594. https://doi.org/10.3389/fpls.2023.1102594.Kalleshwaraswamy C.M., Kannan M., Prakash N.B. Chapter 16 - Silicon as a natural plant guard against insect pests, Editor(s): H. Etesami, A.H. Al Saeedi, H. El-Ramady, M. Fujita, M. Pessarakli, M.A. Hossain, Silicon and Nano-silicon in Environmental Stress Management and Crop Quality Improvement, Academic Press, 2022. P.219-227. https://doi.org/10.1016/B978-0-323-91225-9.00004-2Kalleshwaraswamy C.M., Kannan M., Prakash N.B. Chapter 16 - Silicon as a natural plant guard against insect pests, Editor(s): H. Etesami, A.H. Al Saeedi, H. El-Ramady, M. Fujita, M. Pessarakli, M.A. Hossain, Silicon and Nano-silicon in Environmental Stress Management and Crop Quality Improvement, Academic Press, 2022. P.219-227. https://doi.org/10.1016/B978-0-323-91225-9.00004-2Hayat S.H., Ali A.M., Hayat K., Khan M.A., Cheng Z. Aqueous Garlic Extract as a Plant Biostimulant Enhances Physiology, Improves Crop Quality and Metabolite Abundance, and Primes the Defense Responses of Receiver Plants. Appl. Sci. 2018;(8):1505. https://doi.org/10.3390/app8091505Hayat S.H., Ali A.M., Hayat K., Khan M.A., Cheng Z. Aqueous Garlic Extract as a Plant Biostimulant Enhances Physiology, Improves Crop Quality and Metabolite Abundance, and Primes the Defense Responses of Receiver Plants. Appl. Sci. 2018;(8):1505. https://doi.org/10.3390/app8091505Alfthan G.V. A micromethod for the determination of selenium in tissues and biological fluids by single-test-tube fluorimetry. Anal. Chim. Acta. 1984;(165):187–194. https://doi.org/10.1016/s0003-2670(00)85199-5Alfthan G.V. A micromethod for the determination of selenium in tissues and biological fluids by single-test-tube fluorimetry. Anal. Chim. Acta. 1984;(165):187–194. https://doi.org/10.1016/s0003-2670(00)85199-5Golubkina N.A., Kekina H.G., Molchanova A.V., Antoshkina M.S., Nadezhkin S.M., Soldatenko A.V. Plants antioxidants and methods of their determination. Infra M: Moscow, Russia,2020. 181 p. ISBN 978-5-16- 015666-8. https://doi.org/10.12737/1045420. https://elibrary.ru/vtgigm (In Russ.)Golubkina N.A., Kekina H.G., Molchanova A.V., Antoshkina M.S., Nadezhkin S.M., Soldatenko A.V. Plants antioxidants and methods of their determination. Infra M: Moscow, Russia,2020. 181 p. ISBN 978-5-16- 015666-8. https://doi.org/10.12737/1045420. https://elibrary.ru/vtgigm (In Russ.)AOAC Association Official Analytical Chemists. The Official Methods of Analysis of AOAC International; 22 ‘Vitamin C’.; AOAC: Rockville, MD, USA, 2012.AOAC Association Official Analytical Chemists. The Official Methods of Analysis of AOAC International; 22 ‘Vitamin C’.; AOAC: Rockville, MD, USA, 2012.Swamy P.M. Laboratory manual on biotechnology. 2008, Rastogi Publications: Meerut, India, 617.Swamy P.M. Laboratory manual on biotechnology. 2008, Rastogi Publications: Meerut, India, 617.Application Bulletin 121/2 e Metrohm International Headquarters Determination of nitrate with an ion-selective electrode; 5–10.Application Bulletin 121/2 e Metrohm International Headquarters Determination of nitrate with an ion-selective electrode; 5–10.Chase S. Repelling Contarinia Nasturtii (diptera: Cecidomyiidae), A Brassica Specialist, Using Non-Host Essential Oils 2019. Graduate College Dissertations and Theses. 2018. https://scholarworks.uvm.edu/graddis/1018.Chase S. Repelling Contarinia Nasturtii (diptera: Cecidomyiidae), A Brassica Specialist, Using Non-Host Essential Oils 2019. Graduate College Dissertations and Theses. 2018. https://scholarworks.uvm.edu/graddis/1018.Golubkina N., Sheshnitsan S., Kapitalchuk M. Ecological Importance of Insects in Selenium Biogenic Cycling. Int. J. Ecol. 2014;(2014):835636. https://doi.org/10.1155/2014/835636.Golubkina N., Sheshnitsan S., Kapitalchuk M. Ecological Importance of Insects in Selenium Biogenic Cycling. Int. J. Ecol. 2014;(2014):835636. https://doi.org/10.1155/2014/835636.Golubkina N., Skryabin K. Anomalous accumulation of selenium by genetically modified potato, stable to Colorado beetle. J. Food Comp. Anal. 2010;(23):190–193. https://doi.org/10.1016/j.jfca.2009.08.011Golubkina N., Skryabin K. Anomalous accumulation of selenium by genetically modified potato, stable to Colorado beetle. J. Food Comp. Anal. 2010;(23):190–193. https://doi.org/10.1016/j.jfca.2009.08.011Golubkina N., Kharchenko V., Moldovan A., Antoshkina M., Ushakova O., Sękara A., Stoleru V., Murariu O.C., Tallarita A.V., Sannino M. Effect of Selenium and Garlic Extract Treatments of Seed-Addressed Lettuce Plants on Biofortification Level, Seed Productivity and Mature Plant Yield and Quality. Plants. 2024;(13):1190. https://doi.org/10.3390/plants13091190Golubkina N., Kharchenko V., Moldovan A., Antoshkina M., Ushakova O., Sękara A., Stoleru V., Murariu O.C., Tallarita A.V., Sannino M. Effect of Selenium and Garlic Extract Treatments of Seed-Addressed Lettuce Plants on Biofortification Level, Seed Productivity and Mature Plant Yield and Quality. Plants. 2024;(13):1190. https://doi.org/10.3390/plants13091190War A.R., Paulraj M.G., Ahmad T., Buhroo A.A., Hussain B., Ignacimuthu S., Sharma H.C. Mechanisms of plant defense against insect herbivores. Plant Signal Behav. 2012;7(10)1306-20. https://doi.org/10.4161/psb.21663.War A.R., Paulraj M.G., Ahmad T., Buhroo A.A., Hussain B., Ignacimuthu S., Sharma H.C. Mechanisms of plant defense against insect herbivores. Plant Signal Behav. 2012;7(10)1306-20. https://doi.org/10.4161/psb.21663.Golubkina N., Kharchenko V., Moldovan A., Zayachkovsky V., Stepanov V., Pivovarov V., Sekara A., Tallarita A., Caruso G. Nutritional Value of Apiaceae Seeds as Affected by 11 Species and 43 Cultivars. Horticulturae. 2021;(7):57. https://doi.org/10.3390/horticulturae 7030057.Golubkina N., Kharchenko V., Moldovan A., Zayachkovsky V., Stepanov V., Pivovarov V., Sekara A., Tallarita A., Caruso G. Nutritional Value of Apiaceae Seeds as Affected by 11 Species and 43 Cultivars. Horticulturae. 2021;(7):57. https://doi.org/10.3390/horticulturae 7030057.de Souza Vieira T.M.F., Shimano M.Y.H., da Silva Lima R., de Camargo A.C. Optimization of extraction of antioxidants from aromatic herbs and their synergistic effects in a lipid model system. J. Food Bioact. 2019;(7):63-72. https://doi.org/10.31665/JFB.2019.7200.de Souza Vieira T.M.F., Shimano M.Y.H., da Silva Lima R., de Camargo A.C. Optimization of extraction of antioxidants from aromatic herbs and their synergistic effects in a lipid model system. J. Food Bioact. 2019;(7):63-72. https://doi.org/10.31665/JFB.2019.7200.Keunen E., Peshev D., Vangronsveld J., Van Den Ende W., Cuypers A. Plant sugars are crucial players in the oxidative challenge during abiotic stress: Extending the traditional concept. Plant Cell Environ. 2013;(36):1242–1255. https://doi.org/10.1111/pce.12061Keunen E., Peshev D., Vangronsveld J., Van Den Ende W., Cuypers A. Plant sugars are crucial players in the oxidative challenge during abiotic stress: Extending the traditional concept. Plant Cell Environ. 2013;(36):1242–1255. https://doi.org/10.1111/pce.12061Chang Y.-A., Dai N.-C., Chen H.-J., Tseng C.-H., Huang S.-T., Wang S.- J. Regulation of rice sucrose transporter 4 gene expression in response to insect herbivore chewing. J. Plant Interactions. 2019;14(1):525–532. https://doi.org/10.1080/17429145.2019.1662099Chang Y.-A., Dai N.-C., Chen H.-J., Tseng C.-H., Huang S.-T., Wang S.- J. Regulation of rice sucrose transporter 4 gene expression in response to insect herbivore chewing. J. Plant Interactions. 2019;14(1):525–532. https://doi.org/10.1080/17429145.2019.1662099War A.R., Paulraj M.G., Ahmad T., Buhroo A.A., Hussain B., Ignacimuthu S., Sharma H.C. Mechanisms of plant defense against insect herbivores. Plant Signal Behav. 2012;(7):1306–1320.War A.R., Paulraj M.G., Ahmad T., Buhroo A.A., Hussain B., Ignacimuthu S., Sharma H.C. Mechanisms of plant defense against insect herbivores. Plant Signal Behav. 2012;(7):1306–1320.Mithöfer A., Maffei M.E.. General mechanisms of plant defense and plant toxins. In: Gopalakrishnakone P., Carlini C., Ligabue-Braun R., editors. Plant toxins. Toxicology. 2017, Dordrecht: Springer. P. 3–24.Mithöfer A., Maffei M.E.. General mechanisms of plant defense and plant toxins. In: Gopalakrishnakone P., Carlini C., Ligabue-Braun R., editors. Plant toxins. Toxicology. 2017, Dordrecht: Springer. P. 3–24.Rolland F., Baena-Gonzalez E., Sheen J. Sugar sensing and signaling in plants: conserved and novel mechanisms. Ann. Rev. Plant Biol. 2006;(57):675–709. https://doi.org/10.1146/annurev.arplant.57.032905.105441Rolland F., Baena-Gonzalez E., Sheen J. Sugar sensing and signaling in plants: conserved and novel mechanisms. Ann. Rev. Plant Biol. 2006;(57):675–709. https://doi.org/10.1146/annurev.arplant.57.032905.105441Schwachtje J., Minchin P.E.H., Jahnke S., van Dongen J.T., Schittko U., Baldwin I.T. SNF1-related kinases allow plants to tolerate herbivory by allocating carbon to roots. Proc. Natl. Acad. Sci. USA. 2006;(103):12935–12940.Schwachtje J., Minchin P.E.H., Jahnke S., van Dongen J.T., Schittko U., Baldwin I.T. SNF1-related kinases allow plants to tolerate herbivory by allocating carbon to roots. Proc. Natl. Acad. Sci. USA. 2006;(103):12935–12940.Schwachtje J., Baldwin I.T. Why Does Herbivore Attack Reconfigure Primary Metabolism? Plant Physiol. 2008;146(3):845–851. https://doi.org/10.1104/pp.107.112490Schwachtje J., Baldwin I.T. Why Does Herbivore Attack Reconfigure Primary Metabolism? Plant Physiol. 2008;146(3):845–851. https://doi.org/10.1104/pp.107.112490Golubkina N., Moldovan A., Fedotov M., Kekina H., Kharchenko V., Folmanis G., Alpatov A., Caruso G. Iodine and selenium biofortification of chervil plants treated with silicon nanoparticles. Plants. 2021;(10):2528. https://doi.org/10.3390/plants10112528Golubkina N., Moldovan A., Fedotov M., Kekina H., Kharchenko V., Folmanis G., Alpatov A., Caruso G. Iodine and selenium biofortification of chervil plants treated with silicon nanoparticles. Plants. 2021;(10):2528. https://doi.org/10.3390/plants10112528

The authors declare that there are no conflicts of interest present.