Beet is considered a difficult breeding object due to its biological peculiarities that make it difficult to obtain hybrid seed progeny. In cross-pollinated crops, the maximum hybridization is achieved by using sterile mother plants, which are extremely rare in the nature. Inbreeding is one of the ways to isolate genotypes with cytoplasmic male sterility, which are controlled by recessive alleles of nuclear genes and S-factor of mitochondrial DNA, from a complex heterogeneous population. The main distinguishing features allowing to identify plants with male sterility are the structure and coloring of anthers, and the quality composition of pollen. The diversity of phenotypic manifestation of marker coloration in sterile anthers is determined by the ratio of different pigments. Diversity of pollen grains of fertile and sterile plants is caused by differences in their development at the late stages of androgenesis. The degree of sterilization varies in different genotypes, which is associated with complex regulatory mechanisms of interaction between the genetic apparatus of the nucleus and the cytoplasm. Along with abnormalities of pollen grain micropopulations, self-fertilization results in morphological changes in the structure of inflorescences and number of flower organs, which has a direct impact on the seed productivity of plants. The studies summarized in this publication are relevant and essential for the search of effective ways to control plant development in ontogenesis and universal selection criteria in the process of creating fundamentally new forms of plants, which is especially important for breeding.