ovoshchiОвощи РоссииVegetable crops of Russia2072-91462618-7132Федеральный научный центр овощеводства10.18619/2072-9146-2022-3-33-38ovoshchi-1978Research ArticleСЕЛЕКЦИЯ, СЕМЕНОВОДСТВО И БИОТЕХНОЛОГИЯ РАСТЕНИЙBREEDING, SEED PRODUCTION AND PLANT BIOTECHNOLOGYСодержание антоцианов в пищевых растениях при тепловой обработке и храненииEffect of heat treatment and storage on anthocyanins levels in food plantshttps://orcid.org/0000-0002-6206-200XКолдаевВ. М.KoldaevV. M.

Владимир Михайлович Колдаев – доктор биологических наук, профессор, ведущий научный сотрудник лаборатории лекарственных растений. Scopus ID 57189999504

690022, г. Владивосток, проспект 100-лет Владивостока, 159/1

Vladimir M. Koldaev – Doc. Sci. (Biology), Professor, Leading Researcher of the Laboratory of Medicinal Plants. Scopus ID 57189999504

159/1, avenue of 100-years Vladivostok, Vladivostok, 690022

https://orcid.org/0000-0002-0682-2801МаняхинА. Ю.ManyakhinA. Yu.

Артем Юрьевич Маняхин – кандидат биологических наук, старший научный сотрудник лаборатории лекарственных растений. Researcher ID F-7439-2017, Scopus ID 36192233600

690022, г. Владивосток, проспект 100-лет Владивостока, 159/1

Artem Yu. Manyakhin – Cand. Sci. (Biology), Senior Researcher, Laboratory of Medicinal Plants. Researcher ID F-7439-2017, Scopus ID 36192233600

159/1, avenue of 100-years Vladivostok, Vladivostok, 690022

mau84@mail.ruФедеральный научный центр Биоразнообразия наземной биоты Восточной Азии ДВО РАНРоссияFederal Scientific Center of the East Asia Terrestrial Biodiversity FEB RASRussian Federation202223062022033338Copyright © Колдаев В.М., Маняхин А.Ю., 20222022Колдаев В.М., Маняхин А.Ю.Koldaev V.M., Manyakhin A.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vegetables.su/jour/article/view/1978Цель

Цель: Полифенольные растительные пигменты антоцианы имеют высокую антиоксидантную активность, снижают риски многих патологических состояний в организме человека. Однако широкое лечебно-профилактическое использование антоцианов ограничивается их деградацией при обработке растительного сырья. Целью работы являлось исследование устойчивости антоцианов спектрофотометрическим методом при тепловой обработке и хранении овощных и ягодных растений.

Методы

Методы: В исследованиях использовали фиолетовый картофель, баклажаны, краснокочанную капусту, фиолетовую морковь, синий лук, красную малину и жимолость голубую. Устойчивость антоцианов определяли по числовым показателям спектров поглощения экстрактов из исследуемых растений.

Результаты

Результаты: Высокие показатели устойчивости 0.623-0.986 получены для антоцианов корнеплодов фиолетовой моркови, луковиц синего лука или листьев краснокочанной капусты, в составе которых основным является антиоксидант цианидин с относительной антиоксидантной активностью, равной 3.49. Низкие показатели устойчивости 0.229-0.23 получены для антоцианов ягод красной малины и клубней фиолетового картофеля, в составе которых присутствуют пеларгонидин или мальвидин с меньшей в 2.49-3.36 раза антиоксидантной активностью по сравнению с цианидином. Установлена закономерная взаимосвязь между устойчивостью и антиоксидантной активностью антоцианов при коэффициенте ранговой корреляции 0.91 (p<0.05). Получено, что за три месяца хранения в бытовом холодильнике содержание антоцианов в фиолетовой моркови, фиолетовом картофеле снижается на 10-15% от исходного, а при хранении других исследованных растений в течение 1.5-2 месяцев это снижение в 2- раза больше.

Заключение

Заключение: Разработанные спектрофотометрические методы целесообразно применять в экспресс-анализе при отборе перспективных растений для промышленного культивирования как сырья для антоциансодержащих фитопрепаратов.

Introduction

Introduction: Anthocyanins, the polyphenolic plant pigments, have high antioxidant activity (AOA), reduce the risks of many pathological conditions in the human body. However, the wide medical and preventive use of anthocyanins is limited by their degradation during processing of plant raw materials. The objective of the work was to study the anthocyanins’ stability by spectrophotometric method during heat treatment and storage of vegetable and berry plants.

Study objects and methods: Purple potatoes, eggplants, red cabbage, purple carrots, blue onions, red raspberries and blue honeysuckle were used in the study. Anthocyanins’ stability was determined by numerical values of extracts’ absorption spectra from the studied plants.

Results and discussion

Results and discussion: High stability indices of 0.623–0.986 were obtained for the anthocyanins of purple carrots, blue onion bulbs or red cabbage leaves whose main component is the antioxidant cyanidin with a relative AOA equal to 3.49. Low stability indices of 0.229-0.23 were obtained for anthocyanins of red raspberry berries and purple potato tubers containing pelargonidin or malvidin with 2.49–3.36 times lower relative AOA than for cyanidin. A regular correlation between stability and AOA of anthocyanins with a rank correlation coefficient of 0.91 (p<0.05) was established.

It was established that during three months of storage in a domestic refrigerator, the content of anthocyanins in purple carrots and purple potatoes decreased by 10–15% of the initial one, and this decrease was 2-3 times greater when other studied plants were stored for 1.5–2 months.

Conclusion

Conclusion: It is advisable to use the developed spectrophotometric methods in the express-analysis at selection of perspective plants for industrial cultivation as raw material for anthocyanin-containing herbal formulation.

спектрофотометрияустойчивость антоциановантоциансодержащие препаратыфиолетовая морковьфиолетовый картофельspectrophotometryanthocyanin resistanceanthocyanin-containing specimenspurple carrotpurple potatoReferencesSmeriglio A., Barreca D., Bellocco E., Trombetta D. Chemistry, pharmacology and health benefits of anthocyanins. Phytotherapy research. 2016;30(8):1265–1286. https://doi.org/10.1002/ptr.5642Smeriglio A., Barreca D., Bellocco E., Trombetta D. Chemistry, pharmacology and health benefits of anthocyanins. Phytotherapy research. 2016;30(8):1265–1286. https://doi.org/10.1002/ptr.5642Azevedo J., Fernandes I., Faria A., Oliveira J., Fernandes A., Freitas V., et al. Antioxidant properties of anthocyanidins, anthocyanidin-3-glucosides and respective portisins. Food Chemistry. 2010;119(2):518-523. https://doi.org/10.1016/j.foodchem.2009.06.050Azevedo J., Fernandes I., Faria A., Oliveira J., Fernandes A., Freitas V., et al. Antioxidant properties of anthocyanidins, anthocyanidin-3-glucosides and respective portisins. Food Chemistry. 2010;119(2):518-523. https://doi.org/10.1016/j.foodchem.2009.06.050Martín J., Kuskoski E.M., Navas M.J., Asuero A.G. Antioxidant capacity of anthocyanin pigments. Flavonoids from biosynthesis to human health. 2017; 205-255. https://doi.org/10.5772/67718Martín J., Kuskoski E.M., Navas M.J., Asuero A.G. Antioxidant capacity of anthocyanin pigments. Flavonoids from biosynthesis to human health. 2017; 205-255. https://doi.org/10.5772/67718Speer H., D’Cunha N.M., Alexopoulos N.I., McKune A.J., Naumovski N. Anthocyanins and human health a focus on oxidative stress, inflammation and disease. Antioxidants. 2020;9(5),366:1-13. https://doi.org/10.3390/antiox9050366Speer H., D’Cunha N.M., Alexopoulos N.I., McKune A.J., Naumovski N. Anthocyanins and human health a focus on oxidative stress, inflammation and disease. Antioxidants. 2020;9(5),366:1-13. https://doi.org/10.3390/antiox9050366Kelsey N., Hulick W., Winter A., Ross E., Linseman D. Neuroprotective effects of anthocyanins on apoptosis induced by mitochondrial oxidative stress. Nutritional Neuroscience. 2011;14(6),249–259. https://doi.org/10.1179/1476830511y.0000000020Kelsey N., Hulick W., Winter A., Ross E., Linseman D. Neuroprotective effects of anthocyanins on apoptosis induced by mitochondrial oxidative stress. Nutritional Neuroscience. 2011;14(6),249–259. https://doi.org/10.1179/1476830511y.0000000020Koldaev V.M., Kropotov A.V. Anthocyanins in practical medicine. Pacific Medical Journal. 2021;3:24-28. (In Russ.).https://doi.org/10.34215/1609-1175-2021-3-24-28Koldaev V.M., Kropotov A.V. Anthocyanins in practical medicine. Pacific Medical Journal. 2021;3:24-28. (In Russ.).https://doi.org/10.34215/1609-1175-2021-3-24-28Li D., Wang P., Luo Y., Zhao M., Chen F. Health benefits of anthocyanins and molecular mechanisms: update from recent decade. Critical Reviews in Food Science and Nutrition. 2015;57(8):1729-1741. https://doi.org/10.1080/10408398.2015.1030064Li D., Wang P., Luo Y., Zhao M., Chen F. Health benefits of anthocyanins and molecular mechanisms: update from recent decade. Critical Reviews in Food Science and Nutrition. 2015;57(8):1729-1741. https://doi.org/10.1080/10408398.2015.1030064Cerletti C., Curtis A., Bracone F., Digesù C., Morganti A.G., Iacoviello L., Gaetano G., Donati M.B. Dietary anthocyanins and health: data from Flora and Athena EU projects. British Journal Clinical Pharmacology. 2016;83(1):103-106. https://doi.org/10.1111/bcp.12943Cerletti C., Curtis A., Bracone F., Digesù C., Morganti A.G., Iacoviello L., Gaetano G., Donati M.B. Dietary anthocyanins and health: data from Flora and Athena EU projects. British Journal Clinical Pharmacology. 2016;83(1):103-106. https://doi.org/10.1111/bcp.12943Cassidy A. Berry anthocyanin intake and cardiovascular health. Molecular Aspects of Medicine. 2018; 61:76-82. https://doi.org/10.1016/j.mam.2017.05.002Cassidy A. Berry anthocyanin intake and cardiovascular health. Molecular Aspects of Medicine. 2018; 61:76-82. https://doi.org/10.1016/j.mam.2017.05.002Patras A., Brunton N.P., O'Donnell C., Tiwari B.K. Effect of thermal processing on anthocyanin stability in foods; mechanisms and kinetics of degradation. Trends in Food Science and Technology. 2010;21(1):3-11. https://doi.org/10.1016/j.tifs.2009.07.004Patras A., Brunton N.P., O'Donnell C., Tiwari B.K. Effect of thermal processing on anthocyanin stability in foods; mechanisms and kinetics of degradation. Trends in Food Science and Technology. 2010;21(1):3-11. https://doi.org/10.1016/j.tifs.2009.07.004Remini H., Dahmoune F., Sahraoui Y., Madani K., Kapranov V.N., Kiselev E.F. Recent advances on stability of athocyfnins. RUDN Journal of Agronomy and Animal Industries. 2018;13(4):257-286. https://doi.org/10.22363/2312-797X-2018-13-4-257-286Remini H., Dahmoune F., Sahraoui Y., Madani K., Kapranov V.N., Kiselev E.F. Recent advances on stability of athocyfnins. RUDN Journal of Agronomy and Animal Industries. 2018;13(4):257-286. https://doi.org/10.22363/2312-797X-2018-13-4-257-286Bueno J.M., Sáez-Plaza P., Ramos-Escudero F., Jimenez A.M., Fett R., Asuero A.G. Analysis and antioxidant capacity of anthocyanin pigments. Part II: Chemical structure, color and intake anthocyanins. Critical Reviews in Analytical Chemistry. 2012;42(2):126-151. https://doi.org/10.1080/10408347.2011.632314Bueno J.M., Sáez-Plaza P., Ramos-Escudero F., Jimenez A.M., Fett R., Asuero A.G. Analysis and antioxidant capacity of anthocyanin pigments. Part II: Chemical structure, color and intake anthocyanins. Critical Reviews in Analytical Chemistry. 2012;42(2):126-151. https://doi.org/10.1080/10408347.2011.632314Aprelev V.A., Davydova E.V., Smirnov V.A., Panasyuk A.L. Antotsiany. Anthocyanins. Methods for determination of anthocyanins. Science and World. 2018;1(55):32–39. (In Russ.)Aprelev V.A., Davydova E.V., Smirnov V.A., Panasyuk A.L. Antotsiany. Anthocyanins. Methods for determination of anthocyanins. Science and World. 2018;1(55):32–39. (In Russ.)Koldaev V.M. Numerical indicators of absorption spectra in plant extract analysis. Moscow: LENAND; 2021. 160 p. (In Russ.)Koldaev V.M. Numerical indicators of absorption spectra in plant extract analysis. Moscow: LENAND; 2021. 160 p. (In Russ.)Composite Simpson’s rule. URL: https://en.m.wikipedia.org/wiki/Simpson’s_27s_rule Date accessed: 16.02.2022Composite Simpson’s rule. URL: https://en.m.wikipedia.org/wiki/Simpson’s_27s_rule Date accessed: 16.02.2022Urbakh V.Y. Biometric methods. Moscow: Nauka; 1964. 416 p. (In Russ.). URL: https://www.studmed.ru/urbah-vyu-biometricheskie-metody_5ad673186ad.html Date accessed: 25.12.2021Urbakh V.Y. Biometric methods. Moscow: Nauka; 1964. 416 p. (In Russ.). URL: https://www.studmed.ru/urbah-vyu-biometricheskie-metody_5ad673186ad.html Date accessed: 25.12.2021Kusznierewicz B., Piekarska A., Mrugalska B., Konieczka P., Namieśnik J., Bartoszek A.. Phenolic composition and antioxidant properties of polish Blue-Berried Honeysuckle genotypes by HPLC-DAD-MS, HPLC postcolumn derivatization with ABTS or FC, and TLC with DPPH visualization. Journal of Agricultural and Food Chemistry. 2012;60(7):1755–1763. https://doi.org/10.1021/jf2039839Kusznierewicz B., Piekarska A., Mrugalska B., Konieczka P., Namieśnik J., Bartoszek A.. Phenolic composition and antioxidant properties of polish Blue-Berried Honeysuckle genotypes by HPLC-DAD-MS, HPLC postcolumn derivatization with ABTS or FC, and TLC with DPPH visualization. Journal of Agricultural and Food Chemistry. 2012;60(7):1755–1763. https://doi.org/10.1021/jf2039839Pantelidis G.E., Vasilakakis M., Manganaris G.A., Diamantidis G. Antioxidant capacity, phenol, anthocyanin and ascorbic acid contents in raspberries, blackberries, red currants, gooseberries and Cornelian cherries. Food chemistry. 2007;102(3):777-783. https://doi.org/10.1016/j.foodchem.2006.06.021Pantelidis G.E., Vasilakakis M., Manganaris G.A., Diamantidis G. Antioxidant capacity, phenol, anthocyanin and ascorbic acid contents in raspberries, blackberries, red currants, gooseberries and Cornelian cherries. Food chemistry. 2007;102(3):777-783. https://doi.org/10.1016/j.foodchem.2006.06.021Sharma M., Kaushik P. Biochemical composition of eggplant fruits: A Review. Applied sciences. 2021;11(15):7078. https://doi.org/10.3390/app11157078Sharma M., Kaushik P. Biochemical composition of eggplant fruits: A Review. Applied sciences. 2021;11(15):7078. https://doi.org/10.3390/app11157078Draghici G.A., Lupu M.A., Borozan A.B., Nica D., Alda S., Alda L., et al. Red cabbage, millennium’s functional food. Journal of Horticulture, Forestry, and Biotechnology. 2013;17(4):52-55.Draghici G.A., Lupu M.A., Borozan A.B., Nica D., Alda S., Alda L., et al. Red cabbage, millennium’s functional food. Journal of Horticulture, Forestry, and Biotechnology. 2013;17(4):52-55.He Q., Zhang Z., Zhang L. Anthocyanin accumulation, antioxidant ability and stability, and a transcriptional analysis of anthocyanin biosynthesis in purple heading Chinese cabbage (Brassica rapa L. ssp. pekinensis). Journal Agricultural and Food Chemistry. 2016;64(1):132-145. https://doi.org/10.1021/acs.jafc.5b04674He Q., Zhang Z., Zhang L. Anthocyanin accumulation, antioxidant ability and stability, and a transcriptional analysis of anthocyanin biosynthesis in purple heading Chinese cabbage (Brassica rapa L. ssp. pekinensis). Journal Agricultural and Food Chemistry. 2016;64(1):132-145. https://doi.org/10.1021/acs.jafc.5b04674Kita A., Bakowska-Barczak A., Hamouz K., Kułakowska K., Lisińska G. The effect of frying on anthocyanin stability and antioxidant activity of crisps from red- and purple-fleshed potatoes (Solanum tuberosum L.). Journal of food composition and analysis. 2013;(32):169-175. http://dx.doi.org/10.1016/j.jfca.2013.09.006Kita A., Bakowska-Barczak A., Hamouz K., Kułakowska K., Lisińska G. The effect of frying on anthocyanin stability and antioxidant activity of crisps from red- and purple-fleshed potatoes (Solanum tuberosum L.). Journal of food composition and analysis. 2013;(32):169-175. http://dx.doi.org/10.1016/j.jfca.2013.09.006Berno N.D., Tezotto-Uliana J.V., dos Santos Dias C.T., Kluge R.A. Storage temperature and type of cut affect the biochemical and physiological characteristics of fresh-cut purple onions. Postharvest Biology and Technology. 2014;(93):91-96. https://doi.org/10.1016/j.postharvbio.2014.02.012Berno N.D., Tezotto-Uliana J.V., dos Santos Dias C.T., Kluge R.A. Storage temperature and type of cut affect the biochemical and physiological characteristics of fresh-cut purple onions. Postharvest Biology and Technology. 2014;(93):91-96. https://doi.org/10.1016/j.postharvbio.2014.02.012Zhang S.l., Deng P., Xu Y..C, Lü S.W., Wang J.J. Quantification and analysis of anthocyanin and flavonoids compositions, and antioxidant activities in onions with three different colors. Journal of Integrative Agriculture. 2016;15(9):2175-2181. https://doi.org/10.1016/S2095-3119(16)61385-0Zhang S.l., Deng P., Xu Y..C, Lü S.W., Wang J.J. Quantification and analysis of anthocyanin and flavonoids compositions, and antioxidant activities in onions with three different colors. Journal of Integrative Agriculture. 2016;15(9):2175-2181. https://doi.org/10.1016/S2095-3119(16)61385-0Lee E.J., Yoo K.S., Patil B.S. Total carotenoid, anthocyanin, and sugar contents in sliced or whole purple (cv. Betasweet) and orange carrots during 4-week cold storage. Horticulture, Environment and Biotechnology. 2011;(52): Article number: 402. https://doi.org/10.1007/s13580-011-0227-0Lee E.J., Yoo K.S., Patil B.S. Total carotenoid, anthocyanin, and sugar contents in sliced or whole purple (cv. Betasweet) and orange carrots during 4-week cold storage. Horticulture, Environment and Biotechnology. 2011;(52): Article number: 402. https://doi.org/10.1007/s13580-011-0227-0Wang H., Cao G., Prior R.L. Oxygen radical absorption capacity of anthocyanins. Journal of Agricultural and Food Chemistry. 1997;45(2):304-309. https://doi.org/10.1021/jf960421tWang H., Cao G., Prior R.L. Oxygen radical absorption capacity of anthocyanins. Journal of Agricultural and Food Chemistry. 1997;45(2):304-309. https://doi.org/10.1021/jf960421tMacura R., Michalczyk M., Fiutak G., Maciejaszek I. Effect of freeze-drying and air-drying on the content of carotenoids and anthocyanins in stored purple carrot. Acta Scientiarum Polonorum, Technologia Alimentaria. 2019;18(2):135-142. http://dx.doi.org/10.17306/J.AFS.2019.0637Macura R., Michalczyk M., Fiutak G., Maciejaszek I. Effect of freeze-drying and air-drying on the content of carotenoids and anthocyanins in stored purple carrot. Acta Scientiarum Polonorum, Technologia Alimentaria. 2019;18(2):135-142. http://dx.doi.org/10.17306/J.AFS.2019.0637Ahmadiani N., Robbins R.J., Collins T.M., Giusti M.M. Molar absorptivity (ε) and spectral characteristics of cyanidin-based anthocyanins from red cabbage. Food chemistry. 2016;197(A):900-906. https://doi.org/10.1016/j.foodchem.2015.11.032Ahmadiani N., Robbins R.J., Collins T.M., Giusti M.M. Molar absorptivity (ε) and spectral characteristics of cyanidin-based anthocyanins from red cabbage. Food chemistry. 2016;197(A):900-906. https://doi.org/10.1016/j.foodchem.2015.11.032Chaovanalikit A., Thompson M.M., Ronald E., Wrolstad R.E. Characterization and quantification of anthocyanins and polyphenolics in blue honeysuckle (Lonicera caerulea L.). Journal of Agricultural and Food Chemistry. 2004;52(4):848–852. https://doi.org/10.1021/jf030509oChaovanalikit A., Thompson M.M., Ronald E., Wrolstad R.E. Characterization and quantification of anthocyanins and polyphenolics in blue honeysuckle (Lonicera caerulea L.). Journal of Agricultural and Food Chemistry. 2004;52(4):848–852. https://doi.org/10.1021/jf030509oBowen-Forbes СS, ZhangY, Nair MG. Anthocyanin content, antioxidant, antiinflammatory and anticancer properties of blackberry and raspberry fruits. Journal of Food Composition and Analysis. 2010; 23(6): 554-560. https://doi.org/10.1016/j.jfca.2009.08.012Bowen-Forbes СS, ZhangY, Nair MG. Anthocyanin content, antioxidant, antiinflammatory and anticancer properties of blackberry and raspberry fruits. Journal of Food Composition and Analysis. 2010; 23(6): 554-560. https://doi.org/10.1016/j.jfca.2009.08.012Zhang Y., Sun Y., Zhang H., Mai Q., Zhang B., Li H., Deng Z. The degradation rules of anthocyanins from eggplant peel and antioxidant capacity in fortified model food system during the thermal treatments. Food Bioscience. 2020;(38):100701. https://doi.org/10.1016/j.fbio.2020.100701Zhang Y., Sun Y., Zhang H., Mai Q., Zhang B., Li H., Deng Z. The degradation rules of anthocyanins from eggplant peel and antioxidant capacity in fortified model food system during the thermal treatments. Food Bioscience. 2020;(38):100701. https://doi.org/10.1016/j.fbio.2020.100701Zhushman AI. Modified starches. Moscow: Pishchepromizdat. 2007. 234 p. (In Russ.)Zhushman AI. Modified starches. Moscow: Pishchepromizdat. 2007. 234 p. (In Russ.)

The authors declare that there are no conflicts of interest present.