ovoshchiОвощи РоссииVegetable crops of Russia2072-91462618-7132Федеральный научный центр овощеводства10.18619/2072-9146-2011-1-28-31ovoshchi-1595Research ArticleСОВРЕМЕННЫЕ НАПРАВЛЕНИЯ СЕЛЕКЦИИ ОВОЩНЫХ КУЛЬТУРMODERN TRENDS IN VEGETABLE CROPS BREEDINGИНДУКЦИЯ ГИНОГЕНЕЗА В КУЛЬТУРЕ НЕОПЫЛЕННЫХ СЕМЯПОЧЕК ТЫКВЫGYNOGENESIS INDUCTION IN CULTURE OF UNPOLLINATED OVULES OF PUMPKINШмыковаН.А.ShmykovaN.A.vniissok@mail.ruШумилинаД.ВShumilinaD.V.vniissok@mail.ruКушнерёваВ.П.KushnerevaV.P.vniissok@mail.ruХимичГ.А.KhimichG.A.vniissok@mail.ruГНУ Всероссийский НИИ селекции и семеноводства овощных культур РоссельхозакадемииРоссияState Scientific Institution, All-Russian Research Institute of Vegetable Breeding and Seed Production Russian Academy of Agricultural ScienceRussian Federation201130032011012831Copyright © Шмыкова .Н., Шумилина .Д., Кушнерёва .В., Химич .Г., 20112011Шмыкова .Н., Шумилина .Д., Кушнерёва .В., Химич .Г.Шмыкова .Н., Шумилина .Д., Кушнерёва .В., Химич .Г.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vegetables.su/jour/article/view/1595

В статье показана перспектива использования культуры неопыленных семяпочек in vitro для получения удвоенных гаплоидов тыквы. Семяпочки 5 гибридов культивировали на питательной среде СВМ. Для индукции гиногенеза использовали два варианта регуляторов роста: первый 0,2 мг/л тидиазурона в сочетании с 0,0001 мкМ эпибрассинолида, второй - 2 мг/л 2,4Д. Частота образования эмбриоидов зависела от варианта сред и сортообразца. Максимальное число эмбриоидов, образующееся из неопыленных семяпочек одной завязи составляло 6-9 штук.

The perspective of utilization of in vitro culture of unpollinated ovules for development of dihaploid lines of pumpkin is described in the article. The ovules of five hybrids were cultivated on CBM medium. The following combinations of growth regulators for gynogenesis induction were used: a) 0.2 mg/L of thidiazuron and 0,0001 мкМ of epibrassinolide; b) 2 mg/l of 2,4-D. The frequency of embryo development depended on cultivation medium and genotype. The maximum amount of embryos developed from unpollinated ovules of one ovary was 6-9 ones.

culture of unpollinated ovulesgynogenesishaploid plants of pumpkinкультура неопыленных семяпочекгиногенезгаплоиды тыквыReferencesШмыкова Н.А., Супрунова Т.П. Индукция гиногенеза в культуре in vitro неопыленных семяпочек Cucumis sativus L. // Гавриш - 2009. -№4. - С.40-44.Шмыкова Н.А., Супрунова Т.П. Индукция гиногенеза в культуре in vitro неопыленных семяпочек Cucumis sativus L. // Гавриш - 2009. -№4. - С.40-44.Blakeslee A.F., Belling J. Chromosomal mutations in the Jion weed, Datura stramonium //J. Heredity. - 1924. - Vol. 15. - P. 195 - 206.Blakeslee A.F., Belling J. Chromosomal mutations in the Jion weed, Datura stramonium //J. Heredity. - 1924. - Vol. 15. - P. 195 - 206.Caglar G., Abak K., Progress in the production of haploid embryos, plant and double haploids in cucumber (C. sativus L.) by gamma irradiated pollen in Turkey. //Acta Hort., 1999. -Vol. 492.- P. 317-322.Caglar G., Abak K., Progress in the production of haploid embryos, plant and double haploids in cucumber (C. sativus L.) by gamma irradiated pollen in Turkey. //Acta Hort., 1999. -Vol. 492.- P. 317-322.Dryanovska O.A. Induced callus in vitro from ovaries and anthers of species from the Cucurbitaceae family. //C.R. Acad. Bulgar. Sci.- 1985.- 38. - P. 1243-1244.Dryanovska O.A. Induced callus in vitro from ovaries and anthers of species from the Cucurbitaceae family. //C.R. Acad. Bulgar. Sci.- 1985.- 38. - P. 1243-1244.Gemes Juhasz, Balogh P., Ferenczy A., Kristof Z. Effect of optimal stage of female gametophyte and heat treatment on in vitro Gynogenesis induction in cucumber (Cucumis sativus L.). //Plant Cell Rep. - 2002. - Vol. 21. - P. 105-111.Gemes Juhasz, Balogh P., Ferenczy A., Kristof Z. Effect of optimal stage of female gametophyte and heat treatment on in vitro Gynogenesis induction in cucumber (Cucumis sativus L.). //Plant Cell Rep. - 2002. - Vol. 21. - P. 105-111.Ficcadenti N., Veronose P., Sesuli S. et al. Influence of genotype on induction of haploidy in Cucumis melo L. by using irradiated pollen.// J. Genet Breed.1995. - Vol. 49. - P.-359-Ficcadenti N., Veronose P., Sesuli S. et al. Influence of genotype on induction of haploidy in Cucumis melo L. by using irradiated pollen.// J. Genet Breed.1995. - Vol. 49. - P.-359-Gursoz N., Abak K., Pirat M., Rode J.C., Dumas de Vaulx R. Obtention of haploid plants induced by irradiated pollen in watermelon (Citrullus lanatus). //Cucurbit Genetic Coop. 1991.- Vol. 14. - P. 109-110.Gursoz N., Abak K., Pirat M., Rode J.C., Dumas de Vaulx R. Obtention of haploid plants induced by irradiated pollen in watermelon (Citrullus lanatus). //Cucurbit Genetic Coop. 1991.- Vol. 14. - P. 109-110.Kurtar E.S. Research on the effects of genotypes and growing seasons on in situ haploid embryo induction and in vitro plant obtention via irradiated pollen in squash. /PhD Thesis, University of Cukurova, 1999.Kurtar E.S. Research on the effects of genotypes and growing seasons on in situ haploid embryo induction and in vitro plant obtention via irradiated pollen in squash. /PhD Thesis, University of Cukurova, 1999.Kurtar E.S. Influence of gamma irradiated pollen viability, germinability and fruit and seed-set of pumpkin and winter squash. //Afr J Bio. - 2009. - 8. - P. 6918-6926.Kurtar E.S. Influence of gamma irradiated pollen viability, germinability and fruit and seed-set of pumpkin and winter squash. //Afr J Bio. - 2009. - 8. - P. 6918-6926.Kurtar E.S., Sari N., Abak K. Obtention of haploid embryo and plants through irradiated pollen technique in squash (Cucurbita pepo L.). //Euphytica, 2002. - Vol. 127.- P. 335-344.Kurtar E.S., Sari N., Abak K. Obtention of haploid embryo and plants through irradiated pollen technique in squash (Cucurbita pepo L.). //Euphytica, 2002. - Vol. 127.- P. 335-344.Kurtar E.S., Balkaya A., Ozbakir M., Ofluoglu T. Induction of haploid embryo and plant regeneration via irradiated pollen technique in pumpkin (Cucurbita moschata Duchesne ex. Poir). //Afr J Bio. - 2009. 8. - P. 5944- 5951.Kurtar E.S., Balkaya A., Ozbakir M., Ofluoglu T. Induction of haploid embryo and plant regeneration via irradiated pollen technique in pumpkin (Cucurbita moschata Duchesne ex. Poir). //Afr J Bio. - 2009. 8. - P. 5944- 5951.Kurtar E.S, Balkaya A. Production of in vitro haploid plants from in situ induced haploid embryos in winter squash (Cucurbita maxima Duchesne ex. Lam) via irradiated pollen. Plant Cell Tiss. Organ Cult. 2010. DOI 10. 1007/s11240-010-9729-1.Kurtar E.S, Balkaya A. Production of in vitro haploid plants from in situ induced haploid embryos in winter squash (Cucurbita maxima Duchesne ex. Lam) via irradiated pollen. Plant Cell Tiss. Organ Cult. 2010. DOI 10. 1007/s11240-010-9729-1.Lim W., Earle E.D. Enhanced recovery of doubled haploid lines from parthenogenetic plants of melon (Cucumis melo L.). //Plant Cell Rep.,2009. - Vol. 98. - P. 351-356.Lim W., Earle E.D. Enhanced recovery of doubled haploid lines from parthenogenetic plants of melon (Cucumis melo L.). //Plant Cell Rep.,2009. - Vol. 98. - P. 351-356.Lotfi M., Alan A.R., Henning M.J., Jahn M.M., Earle E.D. Production of haploid and doubled haploid plants of melon (Cucumis melo L.) for use in breeding for multiple virus resistance .// Plant Cell Rep., 2003. - Vol. 21.- P. 1121- 1128.Lotfi M., Alan A.R., Henning M.J., Jahn M.M., Earle E.D. Production of haploid and doubled haploid plants of melon (Cucumis melo L.) for use in breeding for multiple virus resistance .// Plant Cell Rep., 2003. - Vol. 21.- P. 1121- 1128.Metwally E. A., Moustafa S.A., El-Sawy B.I., Haroun S.A., Shalaby T.A. Production of haploid from in vitro culture of unpollinated ovules of Cucurbita pepo. //Plant Cell Tiss. Organ Cult. 1998a. -Vol 52. - P. 117-121.Metwally E. A., Moustafa S.A., El-Sawy B.I., Haroun S.A., Shalaby T.A. Production of haploid from in vitro culture of unpollinated ovules of Cucurbita pepo. //Plant Cell Tiss. Organ Cult. 1998a. -Vol 52. - P. 117-121.Metwally E. A., Moustafa S.A., El-Sawy B.I., Haroun S.A., Shalaby T.A. Haploid plantlets derived by anther culture of Cucurbita pepo. //Plant Cell Tiss. Organ Cult., 1998b. - Vol 52.-P. 171-176.Metwally E. A., Moustafa S.A., El-Sawy B.I., Haroun S.A., Shalaby T.A. Haploid plantlets derived by anther culture of Cucurbita pepo. //Plant Cell Tiss. Organ Cult., 1998b. - Vol 52.-P. 171-176.Mohamed M.F., Refaei E.F.S. Enhanced haploid regeneration in anther culture of summer squash Cucurbita pepo L., Cucurbit Genetics Cooperative Report. /2004. - Vol. 27. - P.57-60Mohamed M.F., Refaei E.F.S. Enhanced haploid regeneration in anther culture of summer squash Cucurbita pepo L., Cucurbit Genetics Cooperative Report. /2004. - Vol. 27. - P.57-60Murashige I., Skoog F.A. A reuised medium for rapid growth and bioassays with tabacco tissue cultures // Physiologia plantarum., 1962. - Vol.15. - N 3. - P. 473-497.Murashige I., Skoog F.A. A reuised medium for rapid growth and bioassays with tabacco tissue cultures // Physiologia plantarum., 1962. - Vol.15. - N 3. - P. 473-497.Sary N., Abak K., Pirat M. Comparison ploidy level screening methods in watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai). /Scientia Hort .,1999. - Vol. 82.- P. 265-277.Sary N., Abak K., Pirat M. Comparison ploidy level screening methods in watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai). /Scientia Hort .,1999. - Vol. 82.- P. 265-277.Sauton A. Effect of season and genotype on gynogenetic haploid production in muskmelon, Cucumis melo L. //Sci Hort., 1988. - V.35. - P. 71-75.Sauton A. Effect of season and genotype on gynogenetic haploid production in muskmelon, Cucumis melo L. //Sci Hort., 1988. - V.35. - P. 71-75.Sauton A. Haploid gynogenesis in Cucumis sativus induced by irradiated pollen. /Cucurbit Genetics Cooperative Report, 1989.- Vol. 12. - P. 22-23.Sauton A. Haploid gynogenesis in Cucumis sativus induced by irradiated pollen. /Cucurbit Genetics Cooperative Report, 1989.- Vol. 12. - P. 22-23.Sun Y., Mei S., Peng J.et al. Induced haploid plants after pollination by irradiated pollen in Cucumis melo L. /Hubei Agr Sci., 2006. - Vol. 4. - P. 98-100.Sun Y., Mei S., Peng J.et al. Induced haploid plants after pollination by irradiated pollen in Cucumis melo L. /Hubei Agr Sci., 2006. - Vol. 4. - P. 98-100.Taner K.Y., Yanmaz R., Kunter B. The effects of irradiation dose and harvest period on haploid plant formation via irradiated pollen in snake cucumber ( Cucumber melo var flexuosus Naud.) /IIIrd National Vegetable Culture Symp. Isparta-Turkey, 2000.- P. 177-181.Taner K.Y., Yanmaz R., Kunter B. The effects of irradiation dose and harvest period on haploid plant formation via irradiated pollen in snake cucumber ( Cucumber melo var flexuosus Naud.) /IIIrd National Vegetable Culture Symp. Isparta-Turkey, 2000.- P. 177-181.Xie M., Zhao J., He H., Wu A., Cai R. Induced haploid plants after pollination by irradiated pollen in Cucumis sativus L. //J. Shanghai Jiaotong Univ (Agr sci)., 2005.- N2. - P.45-49.Xie M., Zhao J., He H., Wu A., Cai R. Induced haploid plants after pollination by irradiated pollen in Cucumis sativus L. //J. Shanghai Jiaotong Univ (Agr sci)., 2005.- N2. - P.45-49.

The authors declare that there are no conflicts of interest present.