ovoshchiОвощи РоссииVegetable crops of Russia2072-91462618-7132Федеральный научный центр овощеводства10.18619/2072-9146-2021-1-125-129ovoshchi-1239Research ArticleЗАЩИТА РАСТЕНИЙPLANT PROTECTIONОценка защитных реакций различных генотипов томата при инфицировании вирусными агентамиAssessment of the response reactions of tomatoes depending on the phytosanitary status in condition of infection with viral agentshttps://orcid.org/0000-0003-3702-3583МэрыйЛ. И.MariiL. I.

Лилиана Ивановна Мэрый – кандидат биологических наук., зав. лабораторией

г. Кишинэу, ул. Пэдурий 20, MD 2002

Liliana I. Marii – Cand. Sci. (Biology), Head of the laboratory

Chisinau, 20 Padurii St., MD 2002

institut.gfpp@gmail.comАндроникЛ. И.AndronicL. I.

Лариса Исааковна Андроник – кандидат биологических наук., директор института

г. Кишинэу, ул. Пэдурий 20, MD 2002

Larisa I. Andronic – Cand. Sci. (Biology), Director of the institute

Chisinau, 20 Padurii St., MD 2002

institut.gfpp@gmail.comСмеряС. В.SmereaS. V.

Светлана Васильевна Смеря –кандидат биологических наук., заместитель директора

г. Кишинэу, ул. Пэдурий 20, MD 2002

Svetlana V. Smerea – Cand. Sci. (Biology), Deputy Director

Chisinau, 20 Padurii St., MD 2002

institut.gfpp@gmail.comЕрханИ. Ф.ErhanI. F.

Ирина Федоровна Ерхан – младший начный сотрудник

г. Кишинэу, ул. Пэдурий 20, MD 2002

Irina F. Erhan – Junior Researcher

Chisinau, 20 Padurii St., MD 2002

institut.gfpp@gmail.comИнститут генетики, физиологии и защиты растенийМолдоваInstitute of Genetics, Physiology and Plant Protection Republic of MoldovaMoldova, Republic of20210503202101125129Copyright © Мэрый Л.И., Андроник Л.И., Смеря С.В., Ерхан И.Ф., 20212021Мэрый Л.И., Андроник Л.И., Смеря С.В., Ерхан И.Ф.Marii L.I., Andronic L.I., Smerea S.V., Erhan I.F.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vegetables.su/jour/article/view/1239Актуальность

Актуальность. Окислительные реакции (на основе активности пероксидаз, РОХ; полифенолоксидазы, РРО и содержания перекиси водорода), возникающие в ответ на инфицирование или реинфицирование потомства зараженных растений, зависят от типа взаимодействия хозяин-вирус (чувствительный, толерантный, устойчивый) и характера взаимодействия с вирусной инфекцией (первичное инфицирование, реинфицирование второго и третьего поколений).

Результаты

Результаты. Реинфицирование вирусом аспермии томата (ВАТ) или вирусом табачной мозаики (ВТМ) потомства третьего поколения зараженных растений вызвало дифференциальные флуктуации активности POX по сравнению с потомствами, зараженными во втором поколении для генотипов Craigella (Tm-22/Tm-22) и S.pimpinellifolium, или к увеличению для генотипов Rufina и Craigella (Tm-1/Tm-1). У генотипов, содержащие гены устойчивости, Rufina и Craigella, при первичном инфицировании ВТМ различия по отношению к контролю по PPO не наблюдали, тогда как при заражении ВАТ в системах, характеризующихся восприимчивостью, установили достоверные отличия. В результате инфицирования ВТМ у чувствительного генотипа (Elvira) и толерантного (S. pimpinellifolium) отмечено увеличение значений PPO по сравнению с контролем. Одновременно у большинства генотипов, первично/вторично зараженных ВАТ или ВТМ, выявлены статистически достоверные различия по активности РОХ или РРО, и также накоплению перекиси водорода в листьях растений, что свидетельствует о специфических защитных реакциях генотипов.

Relevance

Relevance. Oxidative reactions (based on peroxidase activity, POX and polyphenol oxidase, PPO; hydrogen peroxide content) that occur in response to infection or reinfection of the off-spring of infected plants depend on the type of host-virus interaction (sensitive, tolerant, stable) and the nature of the interaction with viral infection (primary infection, reinfection of second and third generations).

Results

Results. Reinfection with tomato aspermy virus (TАV) or tobacco mosaic virus (TMV) of the off-spring of third infected tomato generation caused a significant decrease in POX activity compared to successive generations of infected plants (G2) for Craigella (Tm-22/Tm- 22) and S. pimpinellifolium genotypes or an increase for Rufina and Craigella (Tm-1/Tm-1) ones. In genotypes containing resistance genes, Rufina and Craigella, no differences were observed in PPO of TMV primary infection and control, while significant differences were found in case of TAV (susceptibility). As a result of TMV infection, the sensitive (Elvira) and tolerant (S. pimpinellifolium) genotypes showed an increase in PPO values compared to the control. At the same time, in most genotypes primarily / secondarily infected with TAV or TMV, statistically significant differences were revealed in the activity of POX or PPO, as well as the accumulation of hydrogen peroxide in plant leaves, which indicates specific protective reactions of the genotypes.

вирусы растенийантиоксидантные ферментыпероксидазаполифенолоксидазаперекись водородаplant virusesantioxidant enzymesperoxidasepolyphenol oxidasehydrogen peroxideReferencesArnholdt-Schmitt B. Stress-Induced Cell Reprogramming. Role for Global Genome Regulation? Plant Physiology. 2004;(136):2579–2586.Arnholdt-Schmitt B. Stress-Induced Cell Reprogramming. Role for Global Genome Regulation? Plant Physiology. 2004;(136):2579–2586.Nath M., Bhatt D., Prasad R. et al. Reactive oxygen species generation-scavenging and signaling during plant-arbuscular Mycorrhizal and Piriformospora indica interaction under stress condition. Front Plant Sci. 2016;(7):1574-1584.Nath M., Bhatt D., Prasad R. et al. Reactive oxygen species generation-scavenging and signaling during plant-arbuscular Mycorrhizal and Piriformospora indica interaction under stress condition. Front Plant Sci. 2016;(7):1574-1584.Taranto F., Pasqualone A., Mangini G. et al. Polyphenol oxidases in crops: Biochemical, physiological and genetic aspects. Int. J. Mol. Sci. 2017;18(2):377-393.Taranto F., Pasqualone A., Mangini G. et al. Polyphenol oxidases in crops: Biochemical, physiological and genetic aspects. Int. J. Mol. Sci. 2017;18(2):377-393.Almagro L., Gómez Ros L., Belchi-Navarro S. et al. Class III peroxidases in plant defense reactions. Journal of Experimental Botany, 2009;60(2):377–390.Almagro L., Gómez Ros L., Belchi-Navarro S. et al. Class III peroxidases in plant defense reactions. Journal of Experimental Botany, 2009;60(2):377–390.Saijo Y., Loo E. Plant immunity in signal integration between biotic and abiotic stress responses. New Phytologist, 2020;(225):87–104.Saijo Y., Loo E. Plant immunity in signal integration between biotic and abiotic stress responses. New Phytologist, 2020;(225):87–104.Kathiria P., Sidler C., Golubov A. et al. Tobacco mosaic virus infection results in an increase in recombination frequency and resistance to viral, bacterial, and fungal pathogens in the progeny of infected tobacco plants. Plant Physiol. 2010;153(4):1859-1870.Kathiria P., Sidler C., Golubov A. et al. Tobacco mosaic virus infection results in an increase in recombination frequency and resistance to viral, bacterial, and fungal pathogens in the progeny of infected tobacco plants. Plant Physiol. 2010;153(4):1859-1870.Boyko A., Kovalchuk I. Genetic and epigenetic effects of plant–pathogen interactions: An evolutionary perspective. Molecular Plant, 2011;4(6):1014–1023.Boyko A., Kovalchuk I. Genetic and epigenetic effects of plant–pathogen interactions: An evolutionary perspective. Molecular Plant, 2011;4(6):1014–1023.Ермаков А.И., Арасимович В.В., Ярош Н.П. и др. Методы биохимического исследования растений. Л.: Агропромиздат. 1987. 430 с.Ермаков А.И., Арасимович В.В., Ярош Н.П. и др. Методы биохимического исследования растений. Л.: Агропромиздат. 1987. 430 с.Hitchborn J.H., Hills G.I. The use of negative staining in the electron microscopic examination of plant viruses in crude extracts. Virology. 1965;(27):528-540.Hitchborn J.H., Hills G.I. The use of negative staining in the electron microscopic examination of plant viruses in crude extracts. Virology. 1965;(27):528-540.Thordal-Christensen H., Zhang Z., Wei Y. and Collinge, D. B. Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction. Plant J. 1997;(11):1187-1194.Thordal-Christensen H., Zhang Z., Wei Y. and Collinge, D. B. Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction. Plant J. 1997;(11):1187-1194.Matkovics B., Szabo L., Varga S. I. Study of host-parasite interaction in tomato plants. Acta Biologica Szeged. 1981;27(1-4):17-23.Matkovics B., Szabo L., Varga S. I. Study of host-parasite interaction in tomato plants. Acta Biologica Szeged. 1981;27(1-4):17-23.Boyko A.,Kathiria P., Zemp F. et al. Transgenerational changes in the genome stability and methylation in pathogen-infected plants: (virus-induced plant genome instability). Nucleic Acids Res. 2007;35(5):1714-1725.Boyko A.,Kathiria P., Zemp F. et al. Transgenerational changes in the genome stability and methylation in pathogen-infected plants: (virus-induced plant genome instability). Nucleic Acids Res. 2007;35(5):1714-1725.Kalischuk M., Johnson D., Kawchuk L. Priming with a double-stranded DNA virus alters Brassica rapa seed architecture and facilitates a defense response. Gene. 2015;557(2):130-137.Kalischuk M., Johnson D., Kawchuk L. Priming with a double-stranded DNA virus alters Brassica rapa seed architecture and facilitates a defense response. Gene. 2015;557(2):130-137.Mandal R., P., Psychogios N. et al. Progeny of tobacco mosaic virus-infected Nicotiana tabacum plants exhibit trans-generational changes in metabolic profiles. Biocatalysis and Agricultural Biotechnology. 2012;1(2):115-123.Mandal R., P., Psychogios N. et al. Progeny of tobacco mosaic virus-infected Nicotiana tabacum plants exhibit trans-generational changes in metabolic profiles. Biocatalysis and Agricultural Biotechnology. 2012;1(2):115-123.Balmer A., Pastor V., Gamir J. et al. The 'prime-one': towards a holistic approach to priming. Trends Plant Sci. 2015;20(7):443-452.Balmer A., Pastor V., Gamir J. et al. The 'prime-one': towards a holistic approach to priming. Trends Plant Sci. 2015;20(7):443-452.Hernández J., Gábor G.M., Clemente-Moreno J. et al. Oxidative stress and antioxidative responses in plant–virus interactions. Physiological and Molecular Plant Pathology. 2016;(94):134-148.Hernández J., Gábor G.M., Clemente-Moreno J. et al. Oxidative stress and antioxidative responses in plant–virus interactions. Physiological and Molecular Plant Pathology. 2016;(94):134-148.Clarke S.F., Guy P.L., Burritt D.J. and Jameson P.E. Changes in the activities of antioxidant enzymes in response to virus infection and hormone treatment. Physiol Plant. 2002;(114):157–164.Clarke S.F., Guy P.L., Burritt D.J. and Jameson P.E. Changes in the activities of antioxidant enzymes in response to virus infection and hormone treatment. Physiol Plant. 2002;(114):157–164.Glingston R. S., Deb R., Kumar S., Nagotu S. Organelle dynamics and viral infections: at cross roads. Microbes and Infection. 2019;21(1):20-32.Glingston R. S., Deb R., Kumar S., Nagotu S. Organelle dynamics and viral infections: at cross roads. Microbes and Infection. 2019;21(1):20-32.Bacsó R., Hafez Y. M., Kiraly Z. and Kiraly L. Inhibition of virus replication and symptom expression by reactive oxygen species in tobacco infected with Tobacco mosaic virus. Acta Phytopathology Entomol Hung. 2011;(46):1–10.Bacsó R., Hafez Y. M., Kiraly Z. and Kiraly L. Inhibition of virus replication and symptom expression by reactive oxygen species in tobacco infected with Tobacco mosaic virus. Acta Phytopathology Entomol Hung. 2011;(46):1–10.Hakmaoui A., Pérez-Bueno M.L., García-Fontana B. et al. Analysis of the antioxidant response of Nicotiana benthamiana to infection with two strains of Pepper mild mottle virus. J. Exp. Bot. 2012;(63):5487-5496.Hakmaoui A., Pérez-Bueno M.L., García-Fontana B. et al. Analysis of the antioxidant response of Nicotiana benthamiana to infection with two strains of Pepper mild mottle virus. J. Exp. Bot. 2012;(63):5487-5496.Bilichak A., Ilnytskyy Y., Woycicki R. et al. The elucidation of stress memory inheritance in Brassica rapa plants. Front Plant Sci. 2015;(6):5-23.Bilichak A., Ilnytskyy Y., Woycicki R. et al. The elucidation of stress memory inheritance in Brassica rapa plants. Front Plant Sci. 2015;(6):5-23.

The authors declare that there are no conflicts of interest present.