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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">ovoshchi</journal-id><journal-title-group><journal-title xml:lang="ru">Овощи России</journal-title><trans-title-group xml:lang="en"><trans-title>Vegetable crops of Russia</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2072-9146</issn><issn pub-type="epub">2618-7132</issn><publisher><publisher-name>Федеральный научный центр овощеводства</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.18619/2072-9146-2020-5-22-28</article-id><article-id custom-type="elpub" pub-id-type="custom">ovoshchi-1135</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>СЕЛЕКЦИЯ И СЕМЕНОВОДСТВО СЕЛЬСКОХОЗЯЙСТВЕННЫХ РАСТЕНИЙ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>BREEDING AND SEED PRODUCTION OF AGRICULTURAL CROPS</subject></subj-group></article-categories><title-group><article-title>Создание высоколикопиновых гибридов томата для теплиц с использованием традиционных методов селекции и молекулярных маркеров</article-title><trans-title-group xml:lang="en"><trans-title>Development of high-lycopene tomato hybrids using conventional breeding techniques and molecular markers</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3743-9426</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Игнатова</surname><given-names>С. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Ignatova</surname><given-names>S. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Игнатова Светлана Ильинична – главный научный сотрудник, доктор с.-х. наук, профессор</p><p>140153, Московская область, Раменский р-н, дер. Верея, стр. 500</p></bio><bio xml:lang="en"><p>Svetlana I. Ignatova – Dr. Sc. (Agriculture), Chief Researcher, professor</p><p>500, Vereya, Ramensky district, Moscow region</p></bio><email xlink:type="simple">svil@bk.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1087-9472</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Бабак</surname><given-names>О. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Babak</surname><given-names>O. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Бабак Ольга Геннадьевна – ведущий научный сотрудник, кандидат биол. наук, доцент</p><p>220072, Минск, ул. Академическая, 27</p></bio><bio xml:lang="en"><p>Olga G. Babak – Cand. Sc. (Biology), Leading Researcher, associated professor</p><p>27 Akademicheskaya Rd, Minsk, 220072</p></bio><email xlink:type="simple">babak_olga@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-0552-3340</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Багирова</surname><given-names>С. Ф.</given-names></name><name name-style="western" xml:lang="en"><surname>Bagirova</surname><given-names>S. F.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Багирова Светлана Фекретовна – руководитель лаборатории, кандидат биол. наук</p><p>7а, ул. Счин, Лондон</p></bio><bio xml:lang="en"><p>Svetlana F. Bagirova – Cand. Sc. (Biology), head of the laboratory</p><p>7a Sheen Rd, London</p></bio><email xlink:type="simple">svetlana.bagirova@yahoo.co.uk</email><xref ref-type="aff" rid="aff-3"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ВНИИ овощеводства – филиал ФГБНУ «Федеральный научный центр овощеводства»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Институт генетики и цитологии НАН Беларуси</institution><country>Беларусь</country></aff><aff xml:lang="en"><institution>Institute of Genetics and Cytology at National Academy of Sciences of Belarus</institution><country>Belarus</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Лаборатория Меморум</institution><country>Великобритания</country></aff><aff xml:lang="en"><institution>Meliorem</institution><country>United Kingdom</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2020</year></pub-date><pub-date pub-type="epub"><day>30</day><month>10</month><year>2020</year></pub-date><volume>0</volume><issue>5</issue><fpage>22</fpage><lpage>28</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Игнатова С.И., Бабак О.Г., Багирова С.Ф., 2020</copyright-statement><copyright-year>2020</copyright-year><copyright-holder xml:lang="ru">Игнатова С.И., Бабак О.Г., Багирова С.Ф.</copyright-holder><copyright-holder xml:lang="en">Ignatova S.I., Babak O.G., Bagirova S.F.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.vegetables.su/jour/article/view/1135">https://www.vegetables.su/jour/article/view/1135</self-uri><abstract><sec><title>Актуальность</title><p>Актуальность. Высокое содержание ликопина в плодах томата является важным селекционным признаком при создании новых сортов и гибридов. Использование молекулярных маркеров к аллелям, детерминирующим биосинтез ликопина, в сочетании с традиционными методами селекции на высокоесодержаниекаротиноидов,ценныетехнологические ивкусовыекачестваплодов,атакже на устойчивость к комплексу патогенов, позволяет оценить потенциал накопления антиоксиданта и более эффективно составлять программы по селекции высоколикопиновых форм для условий защищенного грунта.</p></sec><sec><title>Методика</title><p>Методика. Представлены результаты оценки селекционного материала с различными комбинациями аллелей, детерминирующих накопление каротиноидов, и создания гибридов томата с комплексом ценных признаков – высокой продуктивности, устойчивости к болезням, технологических характеристик и биохимического состава плодов. Метод интрогрессии спонтанных или индуцированных мутаций был использован для повышения уровня каротиноидов (og и hp) и улучшения технологических качеств (nor, alc, rin) плодов. Материалом для исследований являлся коллекционный, мутантный, селекционный, гибридный материал томата и внесенные в Госреестр РФ гибриды томата селекции СС Агрофирмы «Ильинична» – ВНИИО филиал ФГБНУ ФНЦО. ДНК-типирование генов качества плодов выполняли в Институте генетики и цитологии НАН Беларуси.</p></sec><sec><title>Результаты</title><p>Результаты. Созданы отечественные гибриды для промышленных теплиц с высокими вкусовыми и технологическими качествами с использованием поэтапных скрещиваний, позволивших объединить гены nor, rin, alc, приводящие к удлинению сроков хранения с генами B, og, hp1 и др., способствующими увеличению содержания каротиноидов в плодах. Установлено, что при целенаправленном отборе и гибридизации, несмотря на негативное влияние генов nor, rin, alc возможно поднять уровень каротиноидов до средних величин. Подтверждена корреляция уровня ликопина в плодах с повышением температуры и уровня инсоляции. Показано, что розовоплодные формы содержат значительно большее количество ликопина, чем красноплодные. Определены сочетания аллелей структурных генов биосинтеза коротиноидов и регуляторных генов, обеспечивающих максимальное накопление ликопина у гибридов с красными и розовыми плодами, формулы которых различались по составу аллелей генов качества плодов. Выделены гибриды с сочетанием высоких концентраций сахара (°Brix), сухого вещества и максимальных значений ликопина, совместно определяющих отличные вкусовые качества: Прекрасная леди, Оля, Кадриль, Виктория. Новые гибриды F1 для промышленных теплиц: Г950, Г956, Г960, Магистраль и розовоплодный Г12897, превосходили голландский стандарт по продуктивности до 21%, а по вкусовым качествам на 11,8 балла.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Relevance</title><p>Relevance. High lycopene fruit content has been regarded as a very important genetic trait in tomato breeding. Use lycopene molecular markers in combination with conventional breeding techniques allowed us to create hybrids with high lycopene accumulation, excellent organoleptic qualities, high yield production and resistance to pathogens, and to effectively optimize our breeding programmes for commercial greehouses production.</p></sec><sec><title>Material and Methods</title><p>Material and Methods. In this study tomato samples including selected lines and hybrids with various allelic combinations of genes determining carotene accumulation, and other genetic traits, such as disease resistance and yield production were tested. Introgression of spontaneous and induced mutations was used to increase carotenoid levels (og and hp) and improve fruit technological qualities (nor, alc, rin). The research material was tomato collection, mutants, breeding lines and hybrids listed in the State Register Russian Federation tomato hybrids of breeding SS Agrofirm "Ilyinichna" VNIIO branch of the All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center. DNA typing of fruit quality genes was performed at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus.</p></sec><sec><title>Results</title><p>Results. New domestic hybrids for industrial greenhouses, which characterised by improved organoleptic qualities and technological traits were developed with the help of phasedcross-breeding that allowed to combine the genes nor, rin, alc, leading to an extension of the shelf life with the genes B, og, hp1, etc., contributing to an increase in carotenoid content in fruits. It was established that for targeted selection and hybridization, despite the negative influence of the nor, rin, alc genes it is possible to raise the level of carotenoids to average values. Correlation between lycopene concentration in fruits and high temperature and level of insolation was confirmed. It was shown that pink-fruited forms contain significantly more lycopenethanred-fruitedones. Different all eliccombinations of structural genes involved in carotenoids biosynthesis and regulatory genes that provided maximal accumulation of lycopene in hybrid swithred and pink fruits were revealed. Hybrids with the combination of high concentrations of sugar (° Brix), dry matter and maximal lycopene values, combined defining excellent taste were selected: Prekrasnaiya lady, Olya, Quadrille, Victoria. New F1 hybrids one for industrial greenhouses: G950, G956, G960, Magistral and pink fruited G12897, surpassed the Dutch standard in productivity up to 21%, and in tastes/organoleptic qualities for 1-1.8 points.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>томат</kwd><kwd>гибриды</kwd><kwd>линии</kwd><kwd>мутанты</kwd><kwd>ДНК-маркеры</kwd><kwd>качество плодов</kwd><kwd>ликопин</kwd><kwd>каротин</kwd><kwd>устойчивость к био- и абиотическим стрессам</kwd></kwd-group><kwd-group xml:lang="en"><kwd>hybrids</kwd><kwd>lines</kwd><kwd>mutants</kwd><kwd>tomato breeding</kwd><kwd>molecular markers</kwd><kwd>fruit quality</kwd><kwd>lycopene</kwd><kwd>carotenoids</kwd><kwd>carotene</kwd><kwd>resistance to stresses</kwd><kwd>biotic stresses</kwd><kwd>abioti</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Tan H.L. et al. Tomato-based food products for prostate cancer prevention: what have we learned? Cancer Metastasis Rev. 2010;29(3):553-568. doi: 10.1007/s10555-010-9246-z.;</mixed-citation><mixed-citation xml:lang="en">Tan H.L. et al. Tomato-based food products for prostate cancer prevention: what have we learned? Cancer Metastasis Rev. 2010;29(3):553-568. doi: 10.1007/s10555-010-9246-z.;</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Sporn M.B., Liby K.T. Is lycopene an effective agent for preventing prostate cancer? Cancer Prev. Res. 2013;6(5):384-386. doi: 10.1158/1940-6207.CAPR13-0026.</mixed-citation><mixed-citation xml:lang="en">Sporn M.B., Liby K.T. Is lycopene an effective agent for preventing prostate cancer? Cancer Prev. Res. 2013;6(5):384-386. doi: 10.1158/1940-6207.CAPR13-0026.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Nisar N., Li Li, Lu S., Khin N.C., Pogson B.J. Carotenoid metabolism in plants. Mol Plant. 2015 Jan;8(1):68-82. doi: 10.1016/j.molp.2014.12.007.</mixed-citation><mixed-citation xml:lang="en">Nisar N., Li Li, Lu S., Khin N.C., Pogson B.J. Carotenoid metabolism in plants. Mol Plant. 2015 Jan;8(1):68-82. doi: 10.1016/j.molp.2014.12.007.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Sun, T., Yuan, H., Cao, H., Yazdani, M., Tadmor, Y., and Li, L. Carotenoid metabolism in plants: the role of plastids. Mol. Plant. 2018;(11):58–74. doi: 10.1016/j.molp.2017.09.010</mixed-citation><mixed-citation xml:lang="en">Sun, T., Yuan, H., Cao, H., Yazdani, M., Tadmor, Y., and Li, L. Carotenoid metabolism in plants: the role of plastids. Mol. Plant. 2018;(11):58–74. doi: 10.1016/j.molp.2017.09.010</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Ilan Levin, C.H. Ric de Vos, Yaakov Tadmor, Arnaud Bovy, Michal Lieberman, Michal OrenShamir, Orit Segev, Igor Kolotilin, Menachem Keller, Rinat Ovadia, Ayala Meir, and Raoul J. Bino High pigment tomato mutants— more than just lycopene (a review). Israel Journal of Plant Sciences. 2006;(54):179–190.</mixed-citation><mixed-citation xml:lang="en">Ilan Levin, C.H. Ric de Vos, Yaakov Tadmor, Arnaud Bovy, Michal Lieberman, Michal OrenShamir, Orit Segev, Igor Kolotilin, Menachem Keller, Rinat Ovadia, Ayala Meir, and Raoul J. Bino High pigment tomato mutants— more than just lycopene (a review). Israel Journal of Plant Sciences. 2006;(54):179–190.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Labate J.A., Grandillo S., Fulton T., Munos S. Tomato. Genome mapping and molecular breeding in plants. Vol. 5. Vegetables, ed. C. Kole. Berlin, Heidelberg, New York: Springer, 2007:1–126.</mixed-citation><mixed-citation xml:lang="en">Labate J.A., Grandillo S., Fulton T., Munos S. Tomato. Genome mapping and molecular breeding in plants. Vol. 5. Vegetables, ed. C. Kole. Berlin, Heidelberg, New York: Springer, 2007:1–126.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">GenBank. Available at: (accessed 1 Aug 2019).</mixed-citation><mixed-citation xml:lang="en">GenBank. Available at: (accessed 1 Aug 2019).</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Solgenomics. Available at: https://solgenomics.net/ (accessed 1 Aug 2019).</mixed-citation><mixed-citation xml:lang="en">Solgenomics. Available at: https://solgenomics.net/ (accessed 1 Aug 2019).</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Кильчевский А.В., Бабак О.Г., Малышев С.В., Аджиева В.Ф., Некрашевич Н.А., Яцевич К.К., Кондратюк А.В. ДНК-типирование генов качества плодов и устойчивости к болезням томата. Методические рекомендации. Министерство сельского хозяйства и продовольствия Республики Беларусь, Национальная академия наук Беларуси, Институт генетики и цитологии Национальной академии наук Беларуси. Минск, 2016. 41 с.</mixed-citation><mixed-citation xml:lang="en">Kilchevsky A.V., Babak O.G., Malyshev S.V., Adzhieva V.F., Nekrashevich N.A., Yatsevich K.K., Kondratyuk A.V. DNA typing of genes for fruit quality and resistance to tomato diseases. Guidelines. The Ministry of Agriculture and Food of the Republic of Belarus, the National Academy of Sciences of Belarus, the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus. Minsk, 2016. 41 р. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Babak O.G., Nekrashevich N.A., Yatsevich K.K., Мalyshev S.V., Kilchevsky A. V. Genetic bases of tomatо marker-assisted selection in Belarus. Eurobiotech. J. 2018;2(2):128-135, doi:10.2478/ebtj-2018-0017</mixed-citation><mixed-citation xml:lang="en">Babak O.G., Nekrashevich N.A., Yatsevich K.K., Мalyshev S.V., Kilchevsky A. V. Genetic bases of tomatо marker-assisted selection in Belarus. Eurobiotech. J. 2018;2(2):128-135, doi:10.2478/ebtj-2018-0017</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Ronen, G., Carmel-Goren L. , Zamir D. Hirschberg J. An alternative pathway to β-carotene formation in plant chromoplasts discovered by mapbased cloning of Beta and old-gold color mutations in tomato. PNAS. 2000;97(20):11102-11107.</mixed-citation><mixed-citation xml:lang="en">Ronen, G., Carmel-Goren L. , Zamir D. Hirschberg J. An alternative pathway to β-carotene formation in plant chromoplasts discovered by mapbased cloning of Beta and old-gold color mutations in tomato. PNAS. 2000;97(20):11102-11107.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Vrebalov, J., Ruezinsky D., Padmanabhan V., White R., Medrano D., Drake R., Schuch W., Giovannoni J. A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor (Rin) locus. Science. 2002;(296):343–345.</mixed-citation><mixed-citation xml:lang="en">Vrebalov, J., Ruezinsky D., Padmanabhan V., White R., Medrano D., Drake R., Schuch W., Giovannoni J. A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor (Rin) locus. Science. 2002;(296):343–345.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Tigchelaar, E.C., M.L. Tomes, E.A. Kerr, and R.J. Barman. A new fruit ripening mutant, nonripening (nor). Rep. Tomato Genet. Coop. 1973. P.23-33.</mixed-citation><mixed-citation xml:lang="en">Tigchelaar, E.C., M.L. Tomes, E.A. Kerr, and R.J. Barman. A new fruit ripening mutant, nonripening (nor). Rep. Tomato Genet. Coop. 1973. P.23-33.</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Kolotilin, I., Koltai H., Tadmor Y., Bar-Or C., Reuveni M., Meir A., Nahon S., Shlomo H., Chen L., Levin I. Transcriptional profiling of high pigment-2dg tomato mutant links early fruit plastid biogenesis with its overproduction of phytonutrients. Plant Physiology. 2007;(145):389–401.</mixed-citation><mixed-citation xml:lang="en">Kolotilin, I., Koltai H., Tadmor Y., Bar-Or C., Reuveni M., Meir A., Nahon S., Shlomo H., Chen L., Levin I. Transcriptional profiling of high pigment-2dg tomato mutant links early fruit plastid biogenesis with its overproduction of phytonutrients. Plant Physiology. 2007;(145):389–401.</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Yen, H.C., Shelton B.A., Howard L.R., Lee S. , Vrebalov J., Giovannoni J. J. The tomato highpigment (hp) locus maps to chromosome 2 and influences plastome copy number and fruit quality. Theor Appl Genet.1997;(95):1069–1079.</mixed-citation><mixed-citation xml:lang="en">Yen, H.C., Shelton B.A., Howard L.R., Lee S. , Vrebalov J., Giovannoni J. J. The tomato highpigment (hp) locus maps to chromosome 2 and influences plastome copy number and fruit quality. Theor Appl Genet.1997;(95):1069–1079.</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Kim B., Kim N., Kang J., Choi Y., Sim S.-C., Min S.R., Park Y. Single Nucleotide Polymorphisms linked to the SlMYB12 Gene that Controls Fruit Peel Color in Domesticated Tomatoes (Solanum lycopersicum L.). Kor. J. Hort. Sci. Technol. 2015;33(4):566-574.</mixed-citation><mixed-citation xml:lang="en">Kim B., Kim N., Kang J., Choi Y., Sim S.-C., Min S.R., Park Y. Single Nucleotide Polymorphisms linked to the SlMYB12 Gene that Controls Fruit Peel Color in Domesticated Tomatoes (Solanum lycopersicum L.). Kor. J. Hort. Sci. Technol. 2015;33(4):566-574.</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Veerappan K., Jung H.J., Hwang I., Kho K.H., Chung M.Y., Nou I.S. Sequence Variation in SlMYB12 is Associated with Fruit Peel Color in Pink Tomato Cultivar. Hortic. Environ. Biotechnol. 2016;57(3):274-279.</mixed-citation><mixed-citation xml:lang="en">Veerappan K., Jung H.J., Hwang I., Kho K.H., Chung M.Y., Nou I.S. Sequence Variation in SlMYB12 is Associated with Fruit Peel Color in Pink Tomato Cultivar. Hortic. Environ. Biotechnol. 2016;57(3):274-279.</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Голубкина Н.А., Молчанова А.В., Тареева М.М., Бабак О.Г., Некрашевич Н.А., Кондратьева И.Ю. Количественная тонкослойная хроматография в оценке каротиноидного состава томата. Овощи России. 2017;(5):96-99. https://doi.org/10.18619/2072-9146-2017-5-96-99</mixed-citation><mixed-citation xml:lang="en">Golubkina N.A., Mоlchanova A.V., Tareeva M.M., Baback O.G., Nekrashevich N.A., Kondratyeva I.Y. Quantitative thing layer chromatography for evaluation of carotenoid composition of tomatoes Solanum lycopersicum. Vegetable crops of Russia. 2017;(5):96-99. (In Russ.) https://doi.org/10.18619/2072-91462017-5-96-99</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Методические указания по селекции сортов и гибридов томата для открытого и защищенного грунта. М.: ВАСХНИЛ, ВНИИССОК, 1986. 98 с.</mixed-citation><mixed-citation xml:lang="en">Guidelines for the selection of tomato varieties and hybrids for open and protected soil. 1986. 98 p. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Методика селекции томата на устойчивость к мучнистой росе (Oidium lycopersicum Cooke et Massee). РАСХН, ВНИИО, 2005. 28 с.</mixed-citation><mixed-citation xml:lang="en">Methods for the selection of tomato for resistance to powdery mildew (Oidium lycopersicum Cooke et Massee). 2005. 28 p. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Куземинский А.В. Селекционно-генетические исследования мутантных форм томата. Харьков, 2004. 392 с.</mixed-citation><mixed-citation xml:lang="en">Kuzeminsky A.V. Selection and genetic studies of mutant forms of tomato. Kharkov, 2004. 392 р. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Багирова С.Ф., Игнатова С.И. Молекулярные методы в селекции растений. Гавриш. 2012;(2):33-38.</mixed-citation><mixed-citation xml:lang="en">Bagirova S.F., Ignatova S.I. Molecular methods in plant breeding. Gavrish. 2012;2:33-38 (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Bramley P. Regulation of carotenoid formation during tomato fruit ripening and development (Review). Journal of Experimental Botany. 2002;53(377):2107-13 • November 2002, DOI: 10.1093/jxb/erf059</mixed-citation><mixed-citation xml:lang="en">Bramley P. Regulation of carotenoid formation during tomato fruit ripening and development (Review). Journal of Experimental Botany. 2002;53(377):2107-13 • November 2002, DOI: 10.1093/jxb/erf059</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Ilahy R., Imen T., Siddiqui M.W., Montefusco A. When color really matters: horticultural performance and functional quality of high-lycopene tomatoes. Plant Sciences. 2018, DOI: 10.1080/07352689.2018.1465631</mixed-citation><mixed-citation xml:lang="en">Ilahy R., Imen T., Siddiqui M.W., Montefusco A. When color really matters: horticultural performance and functional quality of high-lycopene tomatoes. Plant Sciences. 2018, DOI: 10.1080/07352689.2018.1465631</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
