ovoshchiОвощи РоссииVegetable crops of Russia2072-91462618-7132Федеральный научный центр овощеводства10.18619/2072-9146-2020-3-63-68ovoshchi-1081Research ArticleАГРОХИМИЯAGROCHEMISTRYВлияние внекорневого обогащения капусты цветной селенатом натрия на урожайность, пищевую ценность и антиоксидантный статус растенийEffect of foliar sodium selenite biofortification on cauliflower yield, nutritional value and antioxidant statushttps://orcid.org/0000-0002-5510-4873АнтошкинаМ. С.AntoshkinaM. S.

Антошкина Марина Сергеевна – кандидат с.-х. наук, ст.н.с. лабораторно-аналитического отдела

143072, Московская область, Одинцовский район, п. ВНИИССОК, ул. Селекционная, д. 14

Marina S. Antoshkina – Cand. Sci. (Agriculture), senior researcher,

14, Selectsionnaya str., VNIISSOK, Odintsovo district, Moscow region, Russia, 143072

limont_m@mail.ruhttps://orcid.org/0000-0003-1803-9168ГолубкинаН. А.GolubkinaN. A.

Голубкина Надежда Александровна – доктор с.-х. наук, гл.н.с. лабораторно-аналитического отдела

143072, Московская область, Одинцовский район, п. ВНИИССОК, ул. Селекционная, д. 14

Nadezhda A. Golubkina – Doc. Sci. (Agriculture), Chief researcher,

14, Selectsionnaya str., VNIISSOK, Odintsovo district, Moscow region, Russia, 143072

segolubkina45@gmail.comhttps://orcid.org/0000-0002-0912-5913БондареваЛ. Л.BondarevaL. L.

Бондарева Людмила Леонидовна – доктор с.-х. наук, зав. лаб. селекции и семеноводства капустных культур

143072, Московская область, Одинцовский район, п. ВНИИССОК, ул. Селекционная, д. 14

Lyudmila L. Bondareva – Doc. Sci. (Agriculture), Head of Laboratory of Cole Crop Breeding and Seed Production

14, Selectsionnaya str., VNIISSOK, Odintsovo district, Moscow region, Russia, 143072

lyuda_bondareva@mail.ruФедеральное государственное бюджетное научное учреждение "Федеральный научный центр овощеводства" (ФГБНУ ФНЦО)РоссияFederal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC)Russian Federation202030072020036368Copyright © Антошкина М.С., Голубкина Н.А., Бондарева Л.Л., 20202020Антошкина М.С., Голубкина Н.А., Бондарева Л.Л.Antoshkina M.S., Golubkina N.A., Bondareva L.L.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.vegetables.su/jour/article/view/1081Актуальность

Актуальность. Обогащение сельскохозяйственных растений селеном считается наиболее перспективным путем решения проблемы дефицита селена среди населения многих стран мира.

Материал и методика

Материал и методика. Исследовано внекорневое обогащение капусты цветной сорт Полярная звезда различными дозами селената натрия на урожайность, содержание селена и биохимические показатели органов растений.

Результаты

Результаты. Показано, что обогащение селеном растений повышает урожайность в 1,23-1,31 раз, содержание сахаров – в 1,6 раз, аскорбиновой кислоты – 1,52-2,0 раза, но не влияет достоверно на концентрацию полифенолов и уровень жирорастворимых антиоксидантов. Уровень аккумулирования селена снижался в ряду: соцветия > листья > корни. Селенат натрия в концентрации 75 мг/л увеличивал в 1,9 раза массу листьев капусты цветной и в 1,5 раза – корневой системы. Потребление 100 г обогащенной селеном капусты цветной обеспечивало 100% суточной потребности в селене при использовании раствора 50 мг селената/л, 127% – в условиях применения 75 мг селената/л раствора и 418% – при опрыскивании растений раствором селената натрия концентрации 100 мг/л. Принимая во внимание незначительные различия в содержании сахаров и антиоксидантов в соцветиях капусты, обогащенной разными дозами селена, оптимальным представляется использование раствора селената натрия в концентрации 50 мг/л.

Relevance

Relevance. Biofortification of agricultural crops with selenium is considered to be the most promising method for the human selenium status optimization.

Methods

Methods. Effect of foliar biofortification of cauliflower, Polyarnaya Zvezda cv, with sodium selenate of different concentrations on yield, selenium content and biochemical characteristics of plants were investigated.

Results

Results. Enrichment of plants with selenium increased yield by 1.23-1.31 times, sugar content – 1.6 times, ascorbic acid concentration – 1.52-2 times. On the contrary, the treatment did not affect pholyphenol content and antioxidant activity of ethanolic extracts of plants. Selenium accumulation levels decreased according to inflorescences > leaves > roots. Sodium selenate solution at 75 mg/L concentration increased mass of cauliflower leaves 1.9 times and roots – 1.5 times. Consumption of 100 g of cauliflower fortified with 50 mg/L sodium selenate solution provided 100% of the daily adequate selenium consumption level. Utilization of higher sodium selenate concentrations ensured 127% and 418% of the daily adequate consumption level in case of 75 mg/L and 100 mg/L concentrations respectively. Taking into account insignificant differences between sugar content and antioxidants in cauliflower inflorescences fortified with different doses of selenium the most suitable concentration to be used was 50 mg/L.

капуста цветнаяселенсахараантиоксидантыcauliflowerseleniumcarbohydratesantioxidantsReferencesГолубкина, Н.А., Кекина, Е.Г., Молчанова, А.В., Антошкина, М.С., Надежкин, С.М., Солдатенко, А.В. Антиоксиданты растений и методы их определения. 2020.Golubkina N.A., Kekina H.G., Molchanova A.V., Antoshkina M.S., Nadezhkin S.M., Soldatenko A.V. Plants Antioxidants and Methods of their Determination. M., 2020. (In Russ.)Кидин В.В. Практикум по агрохимии. М., Колос, 2008.Kidin V.V. Workshop on agrochemistry. Moscow, Kolos, 2008. (In Russ.)Adhikari P. Biofortification of selenium in broccoli (Brassica oleracea L. var. italic) and onion (Allium cepa L.). Thesis. 2012, Norwegian University of Life Sciences.Adhikari P. Biofortification of selenium in broccoli (Brassica oleracea L. var. italic) and onion (Allium cepa L.). Thesis. 2012, Norwegian University of Life Sciences.AOAC. The Official Methods of Analysis of the Association of Official Analytical; 2012.AOAC. The Official Methods of Analysis of the Association of Official Analytical; 2012.Avila F.W., Yang Y., Faquin V., Ramos S.J., Guilherme L.R.G., Thannhauser T.W., Li L., Impact of selenium supply on Se-methylselenocysteine and glucosinolate accumulation in selenium-biofortified Brassica sprouts. Food Chem. 2014;(165):578-586.Avila F.W., Yang Y., Faquin V., Ramos S.J., Guilherme L.R.G., Thannhauser T.W., Li L., Impact of selenium supply on Se-methylselenocysteine and glucosinolate accumulation in selenium-biofortified Brassica sprouts. Food Chem. 2014;(165):578-586.Bansal A., Sharma S., Dhillon S.K., Dhillon K.S. Selenium Accumulation and Biochemical Composition of Brassica Grains Grown in Selenateor Selenite-Treated Alkaline Sandy Loam Soil. Commun. Soil Sci, Plan. 2012;43(9):1316-1331; http://dx.doi.org/10.1080/00103624.2012.666306.Bansal A., Sharma S., Dhillon S.K., Dhillon K.S. Selenium Accumulation and Biochemical Composition of Brassica Grains Grown in Selenate- or Selenite-Treated Alkaline Sandy Loam Soil. Commun. Soil Sci, Plan. 2012;43(9):1316-1331; http://dx.doi.org/10.1080/00103624.2012.666306.Bradfield C.A., Chang Y., Bjeldanes L.F. Effects of commonly consumed vegetables on hepatic xenobiotic-metabolizing enzymes in the mouse. Food Chem. Toxicol. 1985;23(10):899–904.Bradfield C.A., Chang Y., Bjeldanes L.F. Effects of commonly consumed vegetables on hepatic xenobiotic-metabolizing enzymes in the mouse. Food Chem. Toxicol. 1985;23(10):899–904.Broadley M., Brown P., Cakmak I., Ma J.F., Rengel Z., Zhao F. Function of nutrients: micronutrients. In Marschner’s Mineral Nutrition of Higher Plants. Academic Press, London. 2012. P.249–269.Broadley M., Brown P., Cakmak I., Ma J.F., Rengel Z., Zhao F. Function of nutrients: micronutrients. In Marschner’s Mineral Nutrition of Higher Plants. Academic Press, London. 2012. P.249–269.Djanaguiraman M., Prasad P.V., Seppänen M. Selenium protects sorghum leaves from oxidative damage under high temperature stress by enhancing antioxidant defense system. Plant Physiol. Biochem. 2010;(48):999–1007. doi: 10.1016/j.plaphy.2010.09.009Djanaguiraman M., Prasad P.V., Seppänen M. Selenium protects sorghum leaves from oxidative damage under high temperature stress by enhancing antioxidant defense system. Plant Physiol. Biochem. 2010;(48):999–1007. doi: 10.1016/j.plaphy.2010.09.009El-Ramady H.R., Alla N.A., Fári M., Domokos-Szabolcsy É. Selenium enriched vegetables as biofortification alternative for alleviating micronutrient malnutrition. Int.J.Hort.Sci. 2014;20(1–2):75 –81. doi: 10.31421/IJHS/20/1-2/1121.El-Ramady H.R., Alla N.A., Fári M., Domokos-Szabolcsy É. Selenium enriched vegetables as biofortification alternative for alleviating micronutrient malnutrition. Int.J.Hort.Sci. 2014;20(1–2):75 –81. doi: 10.31421/IJHS/20/1-2/1121.Fageria N.K., Moraes M.F., Ferreira E.P.B., Knupp A.M., Biofortification of trace elements in food crops for human health. Commun. Soil Sci, Plan. 2012;(43):561-570.Fageria N.K., Moraes M.F., Ferreira E.P.B., Knupp A.M., Biofortification of trace elements in food crops for human health. Commun. Soil Sci, Plan. 2012;(43):561-570.FAOSTAT, “Database,” Agricultural Data, 2004, http://www.fao.org/home/en/.FAOSTAT, “Database,” Agricultural Data, 2004, http://www.fao.org/home/en/.Feng R., Wei C., Tu S. The roles of selenium in protecting plants against abiotic stresses. Environ. Exp. Bot. 2013;(87):58–68. doi: 10.1016/j.envexp-bot.2012.09.002.Feng R., Wei C., Tu S. The roles of selenium in protecting plants against abiotic stresses. Environ. Exp. Bot. 2013;(87):58–68. doi: 10.1016/j.envexp-bot.2012.09.002.Fouad A. Ahmed and Rehab F. M. Ali Bioactive Compounds and Antioxidant Activity of Fresh and Processed White Cauliflower. BioMed. Res. Int. 2013;2013:Article ID 367819; http://dx.doi.org/10.1155/2013/367819.Fouad A. Ahmed and Rehab F. M. Ali Bioactive Compounds and Antioxidant Activity of Fresh and Processed White Cauliflower. BioMed. Res. Int. 2013;2013:Article ID 367819; http://dx.doi.org/10.1155/2013/367819.Hartikainen H., Xue T. The promotive effect of selenium on plant growth as triggered by ultraviolet irradiation. J. Environ. Qual. 1999;(28):1372-1375.Hartikainen H., Xue T. The promotive effect of selenium on plant growth as triggered by ultraviolet irradiation. J. Environ. Qual. 1999;(28):1372-1375.Hartikainen H., Xue T., Piironen V. Selenium as an anti-oxidant and pro-oxidant in ryegrass. Plant Soil. 2000;(25):193–200. doi:10.1023/A:1026512921026.Hartikainen H., Xue T., Piironen V. Selenium as an anti-oxidant and pro-oxidant in ryegrass. Plant Soil. 2000;(25):193–200. doi:10.1023/A:1026512921026.Hajiboland R., Rahmat S., Aliasgharzad N., Hartikainen H. Selenium-induced enhancement in carbohydrate metabolism in nodulated alfalfa (Medicago sativa L.) as related to the glutathione redox state. J. Soil Sci. Plant Nutr. 2015;61(4):676-687. doi: 10.1080/00380768.2015.1032181.Hajiboland R., Rahmat S., Aliasgharzad N., Hartikainen H. Selenium-induced enhancement in carbohydrate metabolism in nodulated alfalfa (Medicago sativa L.) as related to the glutathione redox state. J. Soil Sci. Plant Nutr. 2015;61(4):676-687. doi: 10.1080/00380768.2015.1032181.Heimler D., Vignolini P., Dini M.G., Vincieri F.F., Romani A., Antiradical activity and polyphenol composition of local Brassicaceae edible varieties. Food Chem. 2006;99(3):464–469.Heimler D., Vignolini P., Dini M.G., Vincieri F.F., Romani A., Antiradical activity and polyphenol composition of local Brassicaceae edible varieties. Food Chem. 2006;99(3):464–469.Jahangir M., Kim H.K., Choi Y.H., Verpoorte R. Health-affecting compounds in Brassicaceae. Compr. Rev. Food Sci. F. 2009;8(2):31–43.Jahangir M., Kim H.K., Choi Y.H., Verpoorte R. Health-affecting compounds in Brassicaceae. Compr. Rev. Food Sci. F. 2009;8(2):31–43.Kápolna, E., Laursen K.H., Husted S., Larsen E.H. Bio-fortification and isotopic labelling of Se metabolites in onions and carrots following foliar application of Se and 77 Se. Food Chem. 2012;(133):650–657.Kápolna, E., Laursen K.H., Husted S., Larsen E.H. Bio-fortification and isotopic labelling of Se metabolites in onions and carrots following foliar application of Se and 77 Se. Food Chem. 2012;(133):650–657.Kannan V.R., Bastas K.K. Sustainable Approaches to Controlling Plant Pathogenic Bacteria. CRC press. 2015. 421 p.Kannan V.R., Bastas K.K. Sustainable Approaches to Controlling Plant Pathogenic Bacteria. CRC press. 2015. 421 p.Kornaś A., Filek M., Sieprawska A., Bednarska-Kozakiewicz E., Gawrońska K., Miszalski Z. Foliar application of selenium for protection against the first stages of mycotoxin infection of crop plant leaves. J. Sci. Food Agric. 2019;99(1):482-485. doi:10.1002/jsfa.9145.Kornaś A., Filek M., Sieprawska A., Bednarska-Kozakiewicz E., Gawrońska K., Miszalski Z. Foliar application of selenium for protection against the first stages of mycotoxin infection of crop plant leaves. J. Sci. Food Agric. 2019;99(1):482-485. doi:10.1002/jsfa.9145.Oancea A., Gaspar A., Seciu A.-M., Stefan L.M., Craciunescu O., Georgescu F., Lacatusu R. Development of a new technology for protective biofortification with selenium of Brassica crops. AgroLife Scientific J. 2015;4(2):80-85.Oancea A., Gaspar A., Seciu A.-M., Stefan L.M., Craciunescu O., Georgescu F., Lacatusu R. Development of a new technology for protective biofortification with selenium of Brassica crops. AgroLife Scientific J. 2015;4(2):80-85.Owen R.F., Food Chemistry. Marcel Dekkar. New York, NY, USA, 3rd edition, 1996.Owen R.F., Food Chemistry. Marcel Dekkar. New York, NY, USA, 3rd edition, 1996.Pandey K.B., Rizvi S.I. Plant polyphenols as dietary antioxidants in human health and disease. Oxid. Med. Cell. Longev. 2009;2(5):270–278.Pandey K.B., Rizvi S.I. Plant polyphenols as dietary antioxidants in human health and disease. Oxid. Med. Cell. Longev. 2009;2(5):270–278.Park H.S., Han M.H., Kim G.-Y., Moon S.-K., Kim W.-J., Hwang H.J., Park K.Y., Choi Y.H. Sulforaphane induces reactive species-mediated mitotic arrest and subsequent apoptosis in human bladder cancer 5637 cells. Food Chem.Toxicol. 2014;(64):157-165.Park H.S., Han M.H., Kim G.-Y., Moon S.-K., Kim W.-J., Hwang H.J., Park K.Y., Choi Y.H. Sulforaphane induces reactive species-mediated mitotic arrest and subsequent apoptosis in human bladder cancer 5637 cells. Food Chem.Toxicol. 2014;(64):157-165.Raiola A, Errico A., Petruk G., Monti D.M, Barone A., Rigano M.M. Bioactive Compounds in Brassicaceae Vegetables with a Role in the Prevention of Chronic Diseases. Molecules. 2018;(23):15; doi:10.3390/molecules23010015.Raiola A, Errico A., Petruk G., Monti D.M, Barone A., Rigano M.M. Bioactive Compounds in Brassicaceae Vegetables with a Role in the Prevention of Chronic Diseases. Molecules. 2018;(23):15; doi:10.3390/molecules23010015.Sajedi N., Madani H., Naderi A. Effect of microelements and selenium on superoxide dismutase enzyme, malondialdehyde activity and grain yield maize (Zea mays L.) under water deficit stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2011;(39):153-159.Sajedi N., Madani H., Naderi A. Effect of microelements and selenium on superoxide dismutase enzyme, malondialdehyde activity and grain yield maize (Zea mays L.) under water deficit stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2011;(39):153-159.Samec D., Pavlovic I., Salopek-Sondi B. White cabbage (Brassica oleracea var. capitata f. alba): botanical, phytochemical and pharmacological review. Phytochem. Rev. 2016;(16):117-135. http://dx.doi.org/10.1007/s11101-016-9454-4.Samec D., Pavlovic I., Salopek-Sondi B. White cabbage (Brassica oleracea var. capitata f. alba): botanical, phytochemical and pharmacological review. Phytochem. Rev. 2016;(16):117-135. http://dx.doi.org/10.1007/s11101-016-9454-4.Thiruvengadam M., Chung I.-M. Selenium, putrescine, and cadmium influence health-promoting phytochemicals and molecular-level effects on turnip (Brassica rapa ssp. rapa). Food Chem. 2015;(173):1185-193. doi:10.1016/j.foodchem.2014.10.012.Thiruvengadam M., Chung I.-M. Selenium, putrescine, and cadmium influence health-promoting phytochemicals and molecular-level effects on turnip (Brassica rapa ssp. rapa). Food Chem. 2015;(173):1185-193. doi:10.1016/j.foodchem.2014.10.012.Tortorella S.M., Royce S.G., Licciardi P.V., Karagiannis T.C. Dietary sulforaphane in cancer chemoprevention: the role of epigenetic regulation and HDAC inhibition. Antioxid Redox Sign. 2015;(22):1382-1424.Tortorella S.M., Royce S.G., Licciardi P.V., Karagiannis T.C. Dietary sulforaphane in cancer chemoprevention: the role of epigenetic regulation and HDAC inhibition. Antioxid Redox Sign. 2015;(22):1382-1424.Turakainen M. Selenium and its effects on growth, yield and tuber quality in potatoes. Thesis. 2007. Helsinki.Turakainen M. Selenium and its effects on growth, yield and tuber quality in potatoes. Thesis. 2007. Helsinki.Vallejo F., Tomas-Barberan F.AGarc´ıa-Viguera., C. Phenolic compound contents in edible parts of broccoli inflorescences after domestic cooking. J. Sci. Food Agric. 2003;83(14):1511–1516.Vallejo F., Tomas-Barberan F.AGarc´ıa-Viguera., C. Phenolic compound contents in edible parts of broccoli inflorescences after domestic cooking. J. Sci. Food Agric. 2003;83(14):1511–1516.Utoiu E, Oancea A., Gaspar A., Seciu A.-M., Șrefan L.M., Coroiu V., Craciunescu O., Badiu C.D., Oancea F. Selenium biofortification treatment of cauliflower enhances their content in chemopreventive compounds and in vitro antitumoral activity. Sci. Bul., Series F. Biotechnologies. 2017;(XXI):33-40.Utoiu E, Oancea A., Gaspar A., Seciu A.-M., Șrefan L.M., Coroiu V., Craciunescu O., Badiu C.D., Oancea F. Selenium biofortification treatment of cauliflower enhances their content in chemopreventive compounds and in vitro antitumoral activity. Sci. Bul., Series F. Biotechnologies. 2017;(XXI):33-40.Wang C.Q., Water-stress mitigation by selenium in Trifolium repens L. J. Soil Sci. Plant Nutr. 2011;(174):276-282.Wang C.Q., Water-stress mitigation by selenium in Trifolium repens L. J. Soil Sci. Plant Nutr. 2011;(174):276-282.White P.J., Broadley M.R. Biofortification of crops with seven mineral elements often lacking in human diets – iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytol. 2009;(182):49-84.White P.J., Broadley M.R. Biofortification of crops with seven mineral elements often lacking in human diets – iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytol. 2009;(182):49-84.Xue T., Hartikainen H., Piironen V., Antioxidative and growth-promoting effect of selenium on senescing lettuce. Plant Soil. 2001;(237):55-61.Xue T., Hartikainen H., Piironen V., Antioxidative and growth-promoting effect of selenium on senescing lettuce. Plant Soil. 2001;(237):55-61.Zhang Y., Talalay P. Anticarcinogenic activities of organic isothiocyanates: chemistry and mechanisms. Cancer Res. 1994;54(7):1976s–1981s.Zhang Y., Talalay P. Anticarcinogenic activities of organic isothiocyanates: chemistry and mechanisms. Cancer Res. 1994;54(7):1976s–1981s.

The authors declare that there are no conflicts of interest present.